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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB258
Library SL
(Link to library)
Clone ID SLB258
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SLB258Z
(Link to Original site)
Representative DNA sequence 
>SLB258 (SLB258Q) /CSM/SL/SLB2-C/SLB258Q.Seq.d/
XXXXXXXXXXGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCG
TGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCAC
TGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTT
TGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACC
AGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCA
ACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAACTCCATCAT
GAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCTGGTGGTAC
AACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATC
AACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGG
ATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGA
CGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAATAAATATGTTGT
ATTTTATTTAAACTTAA
sequence update 1998. 3.28
Translated Amino Acid sequence 
---GYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFE
AEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMK
CDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGS
ILASLSTFQQMWISKEEYDESGPSIVHRKCF*tinkyvvfylnl
Translated Amino Acid sequence (All Frames) 
Frame A:
---kvmpyhmpsyv*i*lvvillit**ks*lnvvthsppllkeksseiskkn*ptspstl
klkckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnqlvstkppttps*
nvmlisvkiytvmssylvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvd
ls*lhsqlsnkcgspkknmtnqvhqlstenvskqlinmlyfi*t*


Frame B:
---rlclttchltfrfsws*sy*lhdenln*twllihhhc*krnrqryqrkislrrprl*
s*nanccliisprkiiritrrsshhhr**tfplprsplptiilrygiswyprnhlqlhhe
m*c*yp*rfir*crliwwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwi
ylsftlnfptnvdlqrri*rirsincpqkmflnn**iccilfkl


Frame C:
---GYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFE
AEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMK
CDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGS
ILASLSTFQQMWISKEEYDESGPSIVHRKCF*tinkyvvfylnl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLH788 (SLH788Q) /CSM/SL/SLH7-D/SLH788Q.Seq.d/                       1322   0.0
SLH781 (SLH781Q) /CSM/SL/SLH7-D/SLH781Q.Seq.d/                       1322   0.0
SLG170 (SLG170Q) /CSM/SL/SLG1-C/SLG170Q.Seq.d/                       1322   0.0
SLE508 (SLE508Q) /CSM/SL/SLE5-A/SLE508Q.Seq.d/                       1322   0.0
SLE450 (SLE450Q) /CSM/SL/SLE4-C/SLE450Q.Seq.d/                       1322   0.0
SLE380 (SLE380Q) /CSM/SL/SLE3-D/SLE380Q.Seq.d/                       1322   0.0
SLE208 (SLE208Q) /CSM/SL/SLE2-A/SLE208Q.Seq.d/                       1322   0.0
SLC708 (SLC708Q) /CSM/SL/SLC7-A/SLC708Q.Seq.d/                       1322   0.0
SLB729 (SLB729Q) /CSM/SL/SLB7-B/SLB729Q.Seq.d/                       1322   0.0
SLB707 (SLB707Q) /CSM/SL/SLB7-A/SLB707Q.Seq.d/                       1322   0.0
own update 1999. 2. 6
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU052288) Dictyostelium discoideum slug cDNA, clone SLA745.     1320   0.0    1
(AU039680) Dictyostelium discoideum slug cDNA, clone SLG170.     1320   0.0    1
(AU039439) Dictyostelium discoideum slug cDNA, clone SLH788.     1320   0.0    1
(AU039434) Dictyostelium discoideum slug cDNA, clone SLH781.     1320   0.0    1
(AU035022) Dictyostelium discoideum slug cDNA, clone SLE508.     1320   0.0    1
(AU034988) Dictyostelium discoideum slug cDNA, clone SLE450.     1320   0.0    1
(AU034946) Dictyostelium discoideum slug cDNA, clone SLE380.     1320   0.0    1
(AU034701) Dictyostelium discoideum slug cDNA, clone SLE208.     1320   0.0    1
(AU034336) Dictyostelium discoideum slug cDNA, clone SLC708.     1320   0.0    1
(AU034089) Dictyostelium discoideum slug cDNA, clone SLB729.     1320   0.0    1
dna update 2009. 2. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   418   e-116
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   418   e-116
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        418   e-116
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   414   e-114
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   414   e-114
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   412   e-114
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   410   e-113
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          410   e-113
AY615868_1(AY615868|pid:none) Amastigomonas marina actin gene, p...   407   e-112
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   407   e-112
protein update 2009. 6. 1
PSORT 

 psg: 0.55  gvh: 0.41  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SLB258 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLB258Z
3' end seq. 
>SLB258Z.Seq
----------GAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCG
TGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCAC
TGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTT
TGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACC
AGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCA
ACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAACTCCATCAT
GAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCTGGTGGTAC
AACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATC
AACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGG
ATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGA
CGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAATAAATATGTTGT
ATTTTATTTAAACTTAA
Length of 3' end seq. 667
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -