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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB248
Library SL
(Link to library)
Clone ID SLB248
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11187-1
Original site URL
Representative seq. ID SLB248E
(Link to Original site)
Representative DNA sequence 
>SLB248 (SLB248Q) /CSM/SL/SLB2-B/SLB248Q.Seq.d/
GTTTAACTGCGTTAATGGATCCACCACGTCCAGGTGTACCAGAAGCCATTAGAACTTGTA
AAGAAGCAGGTATTAGAGTTATGATGGTAACTGGTGATCATCCATTGACTGGAACAGCAA
TTGCAAAGCAAGTAGGTATCATTGAAACTGATGAAACTCTTAATGATATTGCTGAAAGAG
AAGGTGTTGATGTACTTAGTTTGGACTTTTCACGTGGTACCTCTATTGCCATTACAGGCT
CAATGTTGGATGATCTCACTAGTGAACAGTGGGACAAGATACTTTCCCTAAGAGAACTAT
GCTTTTGTAGAACCAGCCCAGAACAGAAATTACAAATTGTCGCACACCTTCAAAAACGAG
GAGAAATTGTAGCAGTTACTGGTGATGGTGTCAATGATTCACCTGCTCTCAAGAAGGCTG
ATTTAGGTTGTGCTATGGGTATCACTGGTAGTGATGTAGCAAAAGAAGCAGCATCAATTG
TTTTATTGGATGATAATTTTGCATCAATTATCGCTGGTGTAGAAGAAGGTCGTATGATTT
TCGATAAATTAAAGAAATCAATTTGTTACACTCTCTCTTCAAATATTCCAGAGGCAATTC
CATTCTTTTGTTTCTTTGTACTTCAAATGCCAGTAGCTTTATCAGGTATTTTAATTCTTT
GTATAGATTTGGGTACTGATCTTATTCCTGTAATATCTTATGCATACGAAGGTAGTGAAA
CTGATCTCATGAAAAGAAAACCAAGAAATGTTAAAAAAGATAAATTGGTTAGTCTTAGAT
TGGCTATTTTCTCTTATCTCTGGTTAGGTTGTTGGCAATGTGCTGCTGGTTTCTTAAATT
ATTTCCTCCTTTTCAAGGATTATGGATATAGTGCTTCCGACCTTTACAATGTTTCCTCTA
CCTATTTCAAGAAAGATGCTCCACTTTATGGAGGTCATGATGATGCTAAACAAATTCAAA
TTTTAAATGAAGCTCAAACTGCATATTTCATCGCGATTGTAATAAGTAGAGTTGGGGCTT
GTTTCTGCGCTAAAACTAGAATCATTTCAATTTTCCAACAAGGTTTCGGTAATATGGTAT
TCAATTTCGGTGTTTGTTCTATGCTTGCCATTGCTCTATTCATTGTTCATGTACCAGGTG
TTAGAACTTTCTTTGGTTGTACTATTGTCAGTTATAAATATTGGTTAATTCCAATTCCTT
TTGCTGTATTTTTGGTTGCATCAAATGAATTGAGACTTTGGTTAATAAGAAGATATCCAA
AGTCTACATTTGCTAATCTTTGTGCTTGGTAAATTAAATTAAAATAATATAATAAATATA
AATATAAAAATATCATATAAAAAAAAAA
sequence update 1999. 7.12
Translated Amino Acid sequence 
LTALMDPPRPGVPEAIRTCKEAGIRVMMVTGDHPLTGTAIAKQVGIIETDETLNDIAERE
GVDVLSLDFSRGTSIAITGSMLDDLTSEQWDKILSLRELCFCRTSPEQKLQIVAHLQKRG
EIVAVTGDGVNDSPALKKADLGCAMGITGSDVAKEAASIVLLDDNFASIIAGVEEGRMIF
DKLKKSICYTLSSNIPEAIPFFCFFVLQMPVALSGILILCIDLGTDLIPVISYAYEGSET
DLMKRKPRNVKKDKLVSLRLAIFSYLWLGCWQCAAGFLNYFLLFKDYGYSASDLYNVSST
YFKKDAPLYGGHDDAKQIQILNEAQTAYFIAIVISRVGACFCAKTRIISIFQQGFGNMVF
NFGVCSMLAIALFIVHVPGVRTFFGCTIVSYKYWLIPIPFAVFLVASNELRLWLIRRYPK
STFANLCAW*iklk*ynkykyknii*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
v*lr*wihhvqvyqkplelvkkqvlel*w*lviih*leqqlqsk*vslklmkllmillke
kvlmylvwtfhvvpllplqaqcwmislvnsgtryfp*enyafvepaqnrnyklshtfkne
ekl*qllvmvsmihllsrrli*vvlwvslvvm*qkkqhqlfywmiilhqlslv*kkvv*f
sin*rnqfvtlslqifqrqfhsfvslyfkcq*lyqvf*ffv*iwvlilfl*ylmhtkvvk
lis*kenqemlkkinwlvldwlfslisg*vvgnvllvs*iissfsrimdivlptftmfpl
pisrkmlhfmevmmmlnkfkf*mklklhissrl**velglvsalklesfqfsnkvsviwy
sisvfvlclpllyslfmyqvlelslvvllsvinig*fqflllyfwlhqmn*dfg**ediq
slhllifvlgkln*nniininikisykkk


Frame B:
fncvngsttsrctrsh*nl*rsry*sydgnw*ssidwnsnckasryh*n**ns**yc*kr
rc*ct*fglftwylychyrlnvg*sh**tvgqdtfpkrtmll*nqprteitncrtpsktr
rncssyw*wcq*ftcsqeg*frlcygyhw**cskrssincfig**fcinyrwcrrrsydf
r*ikeinllhslfkysrgnsillflctsnassfiryfnslyrfgy*syscnilcirr**n
*shekktkkc*kr*ig*s*igyfllslvrllamccwflklfppfqglwi*cfrplqcfly
lfqercstlwrs**c*tnsnfk*ssncifhrdcnk*swglflr*n*nhfnfptrfr*ygi
qfrclfyachcsihcsctrc*nflwlyycql*ilvnsnsfccifgcik*ietlvnkkisk
vyic*slclvn*ikii**i*i*kyhikkk


Frame C:
LTALMDPPRPGVPEAIRTCKEAGIRVMMVTGDHPLTGTAIAKQVGIIETDETLNDIAERE
GVDVLSLDFSRGTSIAITGSMLDDLTSEQWDKILSLRELCFCRTSPEQKLQIVAHLQKRG
EIVAVTGDGVNDSPALKKADLGCAMGITGSDVAKEAASIVLLDDNFASIIAGVEEGRMIF
DKLKKSICYTLSSNIPEAIPFFCFFVLQMPVALSGILILCIDLGTDLIPVISYAYEGSET
DLMKRKPRNVKKDKLVSLRLAIFSYLWLGCWQCAAGFLNYFLLFKDYGYSASDLYNVSST
YFKKDAPLYGGHDDAKQIQILNEAQTAYFIAIVISRVGACFCAKTRIISIFQQGFGNMVF
NFGVCSMLAIALFIVHVPGVRTFFGCTIVSYKYWLIPIPFAVFLVASNELRLWLIRRYPK
STFANLCAW*iklk*ynkykyknii*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB248 (SLB248Q) /CSM/SL/SLB2-B/SLB248Q.Seq.d/                       2555   0.0
SHD890 (SHD890Q) /CSM/SH/SHD8-D/SHD890Q.Seq.d/                       1390   0.0
SHA628 (SHA628Q) /CSM/SH/SHA6-B/SHA628Q.Seq.d/                       1386   0.0
AHB320 (AHB320Q) /CSM/AH/AHB3-A/AHB320Q.Seq.d/                       1378   0.0
AHE453 (AHE453Q) /CSM/AH/AHE4-C/AHE453Q.Seq.d/                       1370   0.0
VFC718 (VFC718Q) /CSM/VF/VFC7-A/VFC718Q.Seq.d/                       1366   0.0
SHK293 (SHK293Q) /CSM/SH/SHK2-D/SHK293Q.Seq.d/                       1364   0.0
AHJ887 (AHJ887Q) /CSM/AH/AHJ8-D/AHJ887Q.Seq.d/                       1336   0.0
SHA284 (SHA284Q) /CSM/SH/SHA2-D/SHA284Q.Seq.d/                       1263   0.0
SHB228 (SHB228Q) /CSM/SH/SHB2-B/SHB228Q.Seq.d/                       1257   0.0
own update 2002.12.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ405467) Dictyostelium discoideum cDNA clone:dds31d24, 3' ...  1390   0.0    1
(BJ403499) Dictyostelium discoideum cDNA clone:dds25g08, 3' ...  1386   0.0    1
(BJ348394) Dictyostelium discoideum cDNA clone:dda33h05, 3' ...  1378   0.0    1
(BJ350368) Dictyostelium discoideum cDNA clone:dda39j14, 3' ...  1370   0.0    1
(BJ430087) Dictyostelium discoideum cDNA clone:ddv6d05, 3' e...  1366   0.0    1
(BJ353638) Dictyostelium discoideum cDNA clone:dda50n22, 3' ...  1336   0.0    1
(AU060727) Dictyostelium discoideum slug cDNA, clone SLB248.     1326   0.0    1
(BJ408151) Dictyostelium discoideum cDNA clone:dds44i24, 3' ...  1314   0.0    2
(AU033631) Dictyostelium discoideum slug cDNA, clone SLB248.     1265   0.0    1
(BJ403801) Dictyostelium discoideum cDNA clone:dds26g08, 3' ...  1235   0.0    2
dna update 2009. 2. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T18717(T18717) hypothetical protein B0365.3 - Caenorhabditis ele...   380   e-104
AF164349_1(AF164349|pid:none) Mus musculus strain CD-1 Na,K-ATPa...   378   e-103
(Q9WV27) RecName: Full=Sodium/potassium-transporting ATPase subu...   378   e-103
AY140650_1(AY140650|pid:none) Homarus americanus putative Na+/K+...   377   e-103
(Q13733) RecName: Full=Sodium/potassium-transporting ATPase subu...   376   e-103
U18546_1(U18546|pid:none) Caenorhabditis elegans Na,K-ATPase alp...   376   e-102
AY734484_1(AY734484|pid:none) Hirudo medicinalis Na,K ATPase alp...   375   e-102
BC087015_1(BC087015|pid:none) Rattus norvegicus ATPase, Na+/K+ t...   373   e-102
(Q64541) RecName: Full=Sodium/potassium-transporting ATPase subu...   373   e-102
U70316_1(U70316|pid:none) Dictyostelium discoideum IonA (iona) g...   373   e-101
protein update 2009. 7. 4
PSORT 

 psg: 0.62  gvh: 0.43  alm: 0.23  top: 0.27  tms: 0.27  mit: 0.22  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: endoplasmic reticulum
	 28.0 %: plasma membrane
	 12.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for SLB248 is end
5' end seq. ID SLB248F
5' end seq. 
>SLB248F.Seq
GTTTAACTGCGTTAATGGATCCACCACGTCCAGGTGTACCAGAAGCCATTAGAACTTGTA
AAGAAGCAGGTATTAGAGTTATGATGGTAACTGGTGATCATCCATTGACTGGAACAGCAA
TTGCAAAGCAAGTAGGTATCATTGAAACTGATGAAACTCTTAATGATATTGCTGAAAGAG
AAGGTGTTGATGTACTTAGTTTGGACTTTTCACGTGGTACCTCTATTGCCATTACAGGCT
CAATGTTGGATGATCTCACTAGTGAACAGTGGGACAAGATACTTTCCCTAAGAGAACTAT
GCTTTTGTAGAACCAGCCCAGAACAGAAATTACAAATTGTCGCACACCTTCAAAAACGAG
GAGAAATTGTAGCAGTTACTGGTGATGGTGTCAATGATTCACCTGCTCTCAAGAAGGCTG
ATTTAGGTTGTGCTATGGGTATCACTGGTAGTGATGTAGCAAAAGAAGCAGCATCAATTG
TTTTATTGGATGATAATTTTGCATCAATTATCGCTGGTGTAGAAGAAGGTCGTATGATTT
TCGATAAATTAAAGAAATCAATTTGTTACACTCTCTCTTCAAATATTCCAGAGGCAATTC
CATTCTTTTGTTTCTTTGTACTTCAAATGCCAGTAGCTTTATCAGGTATTTTAATTCTTT
GTATAGATT----------
Length of 5' end seq. 669
3' end seq. ID SLB248Z
3' end seq. 
>SLB248Z.Seq
----------TAATTCTTTGTATAGATTTGGGTACTGATCTTATTCCTGTAATATCTTAT
GCATACGAAGGTAGTGAAACTGATCTCATGAAAAGAAAACCAAGAAATGTTAAAAAAGAT
AAATTGGTTAGTCTTAGATTGGCTATTTTCTCTTATCTCTGGTTAGGTTGTTGGCAATGT
GCTGCTGGTTTCTTAAATTATTTCCTCCTTTTCAAGGATTATGGATATAGTGCTTCCGAC
CTTTACAATGTTTCCTCTACCTATTTCAAGAAAGATGCTCCACTTTATGGAGGTCATGAT
GATGCTAAACAAATTCAAATTTTAAATGAAGCTCAAACTGCATATTTCATCGCGATTGTA
ATAAGTAGAGTTGGGGCTTGTTTCTGCGCTAAAACTAGAATCATTTCAATTTTCCAACAA
GGTTTCGGTAATATGGTATTCAATTTCGGTGTTTGTTCTATGCTTGCCATTGCTCTATTC
ATTGTTCATGTACCAGGTGTTAGAACTTTCTTTGGTTGTACTATTGTCAGTTATAAATAT
TGGTTAATTCCAATTCCTTTTGCTGTATTTTTGGTTGCATCAAATGAATTGAGACTTTGG
TTAATAAGAAGATATCCAAAGTCTACATTTGCTAATCTTTGTGCTTGGTAAATTAAATTA
AAATAATATAATAAATATAAATATAAAAATATCATATAAAAAAAAAA
Length of 3' end seq. 697
Connected seq. ID SLB248P
Connected seq. 
>SLB248P.Seq
GTTTAACTGCGTTAATGGATCCACCACGTCCAGGTGTACCAGAAGCCATTAGAACTTGTA
AAGAAGCAGGTATTAGAGTTATGATGGTAACTGGTGATCATCCATTGACTGGAACAGCAA
TTGCAAAGCAAGTAGGTATCATTGAAACTGATGAAACTCTTAATGATATTGCTGAAAGAG
AAGGTGTTGATGTACTTAGTTTGGACTTTTCACGTGGTACCTCTATTGCCATTACAGGCT
CAATGTTGGATGATCTCACTAGTGAACAGTGGGACAAGATACTTTCCCTAAGAGAACTAT
GCTTTTGTAGAACCAGCCCAGAACAGAAATTACAAATTGTCGCACACCTTCAAAAACGAG
GAGAAATTGTAGCAGTTACTGGTGATGGTGTCAATGATTCACCTGCTCTCAAGAAGGCTG
ATTTAGGTTGTGCTATGGGTATCACTGGTAGTGATGTAGCAAAAGAAGCAGCATCAATTG
TTTTATTGGATGATAATTTTGCATCAATTATCGCTGGTGTAGAAGAAGGTCGTATGATTT
TCGATAAATTAAAGAAATCAATTTGTTACACTCTCTCTTCAAATATTCCAGAGGCAATTC
CATTCTTTTGTTTCTTTGTACTTCAAATGCCAGTAGCTTTATCAGGTATTTTAATTCTTT
GTATAGATT----------TAATTCTTTGTATAGATTTGGGTACTGATCTTATTCCTGTA
ATATCTTATGCATACGAAGGTAGTGAAACTGATCTCATGAAAAGAAAACCAAGAAATGTT
AAAAAAGATAAATTGGTTAGTCTTAGATTGGCTATTTTCTCTTATCTCTGGTTAGGTTGT
TGGCAATGTGCTGCTGGTTTCTTAAATTATTTCCTCCTTTTCAAGGATTATGGATATAGT
GCTTCCGACCTTTACAATGTTTCCTCTACCTATTTCAAGAAAGATGCTCCACTTTATGGA
GGTCATGATGATGCTAAACAAATTCAAATTTTAAATGAAGCTCAAACTGCATATTTCATC
GCGATTGTAATAAGTAGAGTTGGGGCTTGTTTCTGCGCTAAAACTAGAATCATTTCAATT
TTCCAACAAGGTTTCGGTAATATGGTATTCAATTTCGGTGTTTGTTCTATGCTTGCCATT
GCTCTATTCATTGTTCATGTACCAGGTGTTAGAACTTTCTTTGGTTGTACTATTGTCAGT
TATAAATATTGGTTAATTCCAATTCCTTTTGCTGTATTTTTGGTTGCATCAAATGAATTG
AGACTTTGGTTAATAAGAAGATATCCAAAGTCTACATTTGCTAATCTTTGTGCTTGGTAA
ATTAAATTAAAATAATATAATAAATATAAATATAAAAATATCATATAAAAAAAAAA
Length of connected seq. 1366
Full length Seq ID SLB248E
Full length Seq. 
>SLB248E.Seq
GTTTAACTGCGTTAATGGATCCACCACGTCCAGGTGTACCAGAAGCCATTAGAACTTGTA
AAGAAGCAGGTATTAGAGTTATGATGGTAACTGGTGATCATCCATTGACTGGAACAGCAA
TTGCAAAGCAAGTAGGTATCATTGAAACTGATGAAACTCTTAATGATATTGCTGAAAGAG
AAGGTGTTGATGTACTTAGTTTGGACTTTTCACGTGGTACCTCTATTGCCATTACAGGCT
CAATGTTGGATGATCTCACTAGTGAACAGTGGGACAAGATACTTTCCCTAAGAGAACTAT
GCTTTTGTAGAACCAGCCCAGAACAGAAATTACAAATTGTCGCACACCTTCAAAAACGAG
GAGAAATTGTAGCAGTTACTGGTGATGGTGTCAATGATTCACCTGCTCTCAAGAAGGCTG
ATTTAGGTTGTGCTATGGGTATCACTGGTAGTGATGTAGCAAAAGAAGCAGCATCAATTG
TTTTATTGGATGATAATTTTGCATCAATTATCGCTGGTGTAGAAGAAGGTCGTATGATTT
TCGATAAATTAAAGAAATCAATTTGTTACACTCTCTCTTCAAATATTCCAGAGGCAATTC
CATTCTTTTGTTTCTTTGTACTTCAAATGCCAGTAGCTTTATCAGGTATTTTAATTCTTT
GTATAGATTTGGGTACTGATCTTATTCCTGTAATATCTTATGCATACGAAGGTAGTGAAA
CTGATCTCATGAAAAGAAAACCAAGAAATGTTAAAAAAGATAAATTGGTTAGTCTTAGAT
TGGCTATTTTCTCTTATCTCTGGTTAGGTTGTTGGCAATGTGCTGCTGGTTTCTTAAATT
ATTTCCTCCTTTTCAAGGATTATGGATATAGTGCTTCCGACCTTTACAATGTTTCCTCTA
CCTATTTCAAGAAAGATGCTCCACTTTATGGAGGTCATGATGATGCTAAACAAATTCAAA
TTTTAAATGAAGCTCAAACTGCATATTTCATCGCGATTGTAATAAGTAGAGTTGGGGCTT
GTTTCTGCGCTAAAACTAGAATCATTTCAATTTTCCAACAAGGTTTCGGTAATATGGTAT
TCAATTTCGGTGTTTGTTCTATGCTTGCCATTGCTCTATTCATTGTTCATGTACCAGGTG
TTAGAACTTTCTTTGGTTGTACTATTGTCAGTTATAAATATTGGTTAATTCCAATTCCTT
TTGCTGTATTTTTGGTTGCATCAAATGAATTGAGACTTTGGTTAATAAGAAGATATCCAA
AGTCTACATTTGCTAATCTTTGTGCTTGGTAAATTAAATTAAAATAATATAATAAATATA
AATATAAAAATATCATATAAAAAAAAAA
Length of full length seq. 1348