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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB221
Library SL
(Link to library)
Clone ID SLB221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16091-1
Original site URL
Representative seq. ID SLB221Z
(Link to Original site)
Representative DNA sequence 
>SLB221 (SLB221Q) /CSM/SL/SLB2-A/SLB221Q.Seq.d/
XXXXXXXXXXAAGATATTCGTCGTGCTCAACTCGAAATGCAACACCAACAAAATTCAAAA
CTGGTATCCGTCAACAAATGCCACCAACTACGGTAGTGGTCCAGTTTTCTTCACTCCAGC
CCCAGTCAACCCACAAGTTGTCTACCAACAAATGATGCCACGTCCACGTAACTGGAACGG
TCAACCAGTAGGTGTCCCACAAGGTCAATACGCCAATATGAACTATGCCCGTGGTCAACC
ACGTCAAAATGGTCCACGTCAAAACGGTGGTCAACCACGTCAAAACGGTCCACGTCCAGA
CGTTTCAGGTGCTCAACCAATTCCAGTTCAACAACAAACCACTTTGGATGTCGCTCAAAC
TCAACAAGCCGCCTCATCAGCTGAATCTGCTCTTAACCTCCAATCAATCATCAATCTCCC
ATCTCGTGATCAACAAACGTTGCTCTCGGTGAACACCTCTACCCATTAATCCACAACTCT
CAACCAGATTTGGCCGGTAAAATCACAGGTATGCTCTTAGACTCTTTACCAGTTGAAGAA
TTATTCACCCTTACTCAACGTCAAGACTTGTTAGCCGATAAAATCAGAGAAGCCCTTGAA
GTTTTAGGTTCAAACTAAAACCCACCACCAATTATTTAATATATAATAAAAAATATAAAT
AAAAAAACAACTCCAACAACAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
sequence update 1998. 3.28
Translated Amino Acid sequence 
---IFVVLNSKCNTNKIQNWYPSTNATNYGSGPVFFTPAPVNPQVVYQQMMPRPRNWNGQ
PVGVPQGQYANMNYARGQPRQNGPRQNGGQPRQNGPRPDVSGAQPIPVQQQTTLDVAQTQ
QAASSAESALNLQSIINLPSRDQQTLLSVNTSTH*sttlnqiwpvksqvcs*tlyqlkny
spllnvktc*piksekplkf*vqtkthhqlfni**ki*ikkqlqqqlkkkkkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
---rysscstrnatptkfktgirqqmppttvvvqfsslqpqsthklstnk*chvhvtgtv
nq*vshkvntpi*tmpvvnhvkmvhvktvvnhvktvhvqtfqvlnqfqfnnkplwmslkl
nkpphqlnllltsnqssishlvinkrcsr*tplpinpqlstrfgr*nhryalrlfts*ri
ihpystsrlvsr*nqrsp*sfrfklkpttnyliynkkyk*knnsnnn*kkkkkkkkk


Frame B:
---dirraqlemqhqqnsklvsvnkchqlr*wssflhsspsqptsclptndatst*lers
tsrcptrsirqyelcpwsttskwstskrwsttskrstsrrfrcstnsssttnhfgcrsns
tsrlis*ics*ppinhqspis*stnvalgehlyplihnsqpdlagkitgmlldslpveel
ftltqrqdlladkirealevlgsn*npppii*yiikninkkttptttkkkkkkkkk


Frame C:
---IFVVLNSKCNTNKIQNWYPSTNATNYGSGPVFFTPAPVNPQVVYQQMMPRPRNWNGQ
PVGVPQGQYANMNYARGQPRQNGPRQNGGQPRQNGPRPDVSGAQPIPVQQQTTLDVAQTQ
QAASSAESALNLQSIINLPSRDQQTLLSVNTSTH*sttlnqiwpvksqvcs*tlyqlkny
spllnvktc*piksekplkf*vqtkthhqlfni**ki*ikkqlqqqlkkkkkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB221 (SLB221Q) /CSM/SL/SLB2-A/SLB221Q.Seq.d/                       1241   0.0
SLA837 (SLA837Q) /CSM/SL/SLA8-B/SLA837Q.Seq.d/                       1213   0.0
SLE325 (SLE325Q) /CSM/SL/SLE3-B/SLE325Q.Seq.d/                       1185   0.0
SLE683 (SLE683Q) /CSM/SL/SLE6-D/SLE683Q.Seq.d/                       1124   0.0
FC-AP19 (FC-AP19Q) /CSM/FC/FC-AP/FC-AP19Q.Seq.d/                     1114   0.0
FC-269 (FC-269Q) /CSM/CG/CG-FC/FC-269Q.Seq.d/                        1114   0.0
FC-AU08 (FC-AU08Q) /CSM/FC/FC-AU/FC-AU08Q.Seq.d/                     1108   0.0
VFI195 (VFI195Q) /CSM/VF/VFI1-D/VFI195Q.Seq.d/                       1068   0.0
SLG688 (SLG688Q) /CSM/SL/SLG6-D/SLG688Q.Seq.d/                       1051   0.0
SLC747 (SLC747Q) /CSM/SL/SLC7-B/SLC747Q.Seq.d/                       1039   0.0
own update 2001.11.27
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU033604) Dictyostelium discoideum slug cDNA, clone SLB221.     1241   0.0    1
(AU034783) Dictyostelium discoideum slug cDNA, clone SLE325.      769   0.0    3
(AU033447) Dictyostelium discoideum slug cDNA, clone SLA837.      769   0.0    3
(AU034912) Dictyostelium discoideum slug cDNA, clone SLE683.      751   0.0    2
(AU284422) Dictyostelium discoideum gamete cDNA clone:FC-AU0...   728   0.0    2
(C24321) Dictyostelium discoideum gamete cDNA, clone FC-AP19.     686   0.0    3
(BJ432178) Dictyostelium discoideum cDNA clone:ddv17m23, 3' ...   694   0.0    4
(AU039896) Dictyostelium discoideum slug cDNA, clone SLG688.      670   0.0    2
(AU034363) Dictyostelium discoideum slug cDNA, clone SLC747.      658   0.0    2
(AU052196) Dictyostelium discoideum slug cDNA, clone SLA219.      642   0.0    2
dna update 2009. 1.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5KBW2) RecName: Full=Polyadenylate-binding protein, cytoplasmi...    60   2e-10
(Q2UK72) RecName: Full=Polyadenylate-binding protein, cytoplasmi...    50   6e-06
(Q0U1G2) RecName: Full=Polyadenylate-binding protein, cytoplasmi...    54   6e-06
(Q6DEY7) RecName: Full=Embryonic polyadenylate-binding protein; ...    52   1e-05
BC081553_1(BC081553|pid:none) Danio rerio ubiquitin protein liga...    52   2e-05
CR940347_278(CR940347|pid:none) Theileria annulata strain Ankara...    51   4e-05
AM468150_4(AM468150|pid:none) Vitis vinifera contig VV78X086105....    51   4e-05
AF338225_1(AF338225|pid:none) Xenopus laevis embryonic poly(A) b...    50   5e-05
BC059662_1(BC059662|pid:none) Danio rerio poly A binding protein...    50   7e-05
(A1CRM1) RecName: Full=Polyadenylate-binding protein, cytoplasmi...    50   7e-05
protein update 2009. 6. 1
PSORT 

 psg: 0.54  gvh: 0.49  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.16
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for SLB221 is mit
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLB221Z
3' end seq. 
>SLB221Z.Seq
----------AAGATATTCGTCGTGCTCAACTCGAAATGCAACACCAACAAAATTCAAAA
CTGGTATCCGTCAACAAATGCCACCAACTACGGTAGTGGTCCAGTTTTCTTCACTCCAGC
CCCAGTCAACCCACAAGTTGTCTACCAACAAATGATGCCACGTCCACGTAACTGGAACGG
TCAACCAGTAGGTGTCCCACAAGGTCAATACGCCAATATGAACTATGCCCGTGGTCAACC
ACGTCAAAATGGTCCACGTCAAAACGGTGGTCAACCACGTCAAAACGGTCCACGTCCAGA
CGTTTCAGGTGCTCAACCAATTCCAGTTCAACAACAAACCACTTTGGATGTCGCTCAAAC
TCAACAAGCCGCCTCATCAGCTGAATCTGCTCTTAACCTCCAATCAATCATCAATCTCCC
ATCTCGTGATCAACAAACGTTGCTCTCGGTGAACACCTCTACCCATTAATCCACAACTCT
CAACCAGATTTGGCCGGTAAAATCACAGGTATGCTCTTAGACTCTTTACCAGTTGAAGAA
TTATTCACCCTTACTCAACGTCAAGACTTGTTAGCCGATAAAATCAGAGAAGCCCTTGAA
GTTTTAGGTTCAAACTAAAACCCACCACCAATTATTTAATATATAATAAAAAATATAAAT
AAAAAAACAACTCCAACAACAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Length of 3' end seq. 703
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -