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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB144
Library SL
(Link to library)
Clone ID SLB144
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLB144Z
(Link to Original site)
Representative DNA sequence 
>SLB144 (SLB144Q) /CSM/SL/SLB1-B/SLB144Q.Seq.d/
XXXXXXXXXXCCATTACGTATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGTAC
TGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGC
CCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGA
AGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAA
AAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGT
TGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTT
AGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCG
TCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGA
TGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATA
CCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCAT
CAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCC
ATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTTATAATAATTTTAA
sequence update 1998. 3.27
Translated Amino Acid sequence 
---LRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEA
RPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLD
CHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYP
PLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafynnf
Translated Amino Acid sequence (All Frames) 
Frame A:
---hyvfhykmstrsvvsvlfq*vvsklvslnqvwssplpqlvsqlksnqskcitnnsqk
pvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvksmlvthqs*
ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnpslnt
hh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*iiflnk*hfiiil


Frame B:
---itysitrclqdrwyrycssrscrnwyh*trygrhlcpswsln*sqisrnasrttprs
psr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnpcwlltslr
lshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilh*ip
tirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeink*yflinsil**f*


Frame C:
---LRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEA
RPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLD
CHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYP
PLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafynnf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD740 (VSD740Q) /CSM/VS/VSD7-B/VSD740Q.Seq.d/                       1312   0.0
VSD380 (VSD380Q) /CSM/VS/VSD3-D/VSD380Q.Seq.d/                       1312   0.0
VFN666 (VFN666Q) /CSM/VF/VFN6-C/VFN666Q.Seq.d/                       1312   0.0
VFN474 (VFN474Q) /CSM/VF/VFN4-D/VFN474Q.Seq.d/                       1312   0.0
VFN376 (VFN376Q) /CSM/VF/VFN3-D/VFN376Q.Seq.d/                       1312   0.0
VFN329 (VFN329Q) /CSM/VF/VFN3-B/VFN329Q.Seq.d/                       1312   0.0
VFN318 (VFN318Q) /CSM/VF/VFN3-A/VFN318Q.Seq.d/                       1312   0.0
VFN184 (VFN184Q) /CSM/VF/VFN1-D/VFN184Q.Seq.d/                       1312   0.0
VFN174 (VFN174Q) /CSM/VF/VFN1-D/VFN174Q.Seq.d/                       1312   0.0
VFN108 (VFN108Q) /CSM/VF/VFN1-A/VFN108Q.Seq.d/                       1312   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X55973) D. discoideum EF1-I gene for elongation factor 1 al...  1294   0.0    1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...  1294   0.0    1
(AU264497) Dictyostelium discoideum vegetative cDNA clone:VS...  1294   0.0    1
(AU263935) Dictyostelium discoideum vegetative cDNA clone:VS...  1294   0.0    1
(AU052275) Dictyostelium discoideum slug cDNA, clone SLA488.     1294   0.0    1
(AU052219) Dictyostelium discoideum slug cDNA, clone SLA265.     1294   0.0    1
(AU039993) Dictyostelium discoideum slug cDNA, clone SLH395.     1294   0.0    1
(AU039527) Dictyostelium discoideum slug cDNA, clone SLE701.     1294   0.0    1
(AU039519) Dictyostelium discoideum slug cDNA, clone SLA776.     1294   0.0    1
(AU034691) Dictyostelium discoideum slug cDNA, clone SLE193.     1294   0.0    1
dna update 2009. 1.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   403   e-111
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   403   e-111
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   403   e-111
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   325   1e-87
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   323   3e-87
AY643816_1(AY643816|pid:none) Lycogala epidendrum elongation fac...   322   9e-87
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   321   1e-86
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   321   2e-86
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   320   2e-86
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   320   2e-86
protein update 2009. 5.31
PSORT 

 psg: 0.89  gvh: 0.50  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for SLB144 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLB144Z
3' end seq. 
>SLB144Z.Seq
----------CCATTACGTATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGTAC
TGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGC
CCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGA
AGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAA
AAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGT
TGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTT
AGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCG
TCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGA
TGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATA
CCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCAT
CAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCC
ATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTTATAATAATTTTAA
Length of 3' end seq. 710
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -