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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB134
Library SL
(Link to library)
Clone ID SLB134
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLB134E
(Link to Original site)
Representative DNA sequence 
>SLB134 (SLB134Q) /CSM/SL/SLB1-B/SLB134Q.Seq.d/
CTGGTTTAATGATACCAGGTATGGTCGTCACCTTTGCCCTAGCTGGTCTCTCAACTGAAG
TCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAG
GTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACT
CCNNNTACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACC
ATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTG
CCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCA
AAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCC
CATCTCGTCCAANGTGTGTTGAATCNTTCACTGAATACCCACCATTAGGTCGTTTCGCCG
TCAGNGNTNTGAGACAANCCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCC
CAGNTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAANGAANTAANTAANTA
ATATTTCTTAATAAATAGCATTTTNTAATAAAAAAAAAAAAAAAAAAAAAAAAA
sequence update 1999. 6.18
Translated Amino Acid sequence 
GLMIPGMVVTFALAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDS
XYDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAK
EGTAAVVLKNGDAAMVELTPSRPXCVESFTEYPPLGRFAVXXXRQXVAVGVIKSTVKKAP
XKAGDKKGAAAPSKXX*xxnis**iafxnkkkkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
lv**yqvwssplp*lvsqlksnqskcitnnsqkpvqvtm*vstlktfqskklkevwslvt
pxtihhkklknsllklssstiqvksmlvthqs*ivtlltlpvnslkssiklivvlvpslp
kkvlppss*rmvmplwsn*phlvqxvlnxslnthh*vvspsxx*dxpspsvssnqpsrkp
qxkpvirkvllphqkxxx*xiflnk*hfxikkkkkkkk


Frame B:
wfndtrygrhlcpswsln*sqisrnasrttprspsr*qcrfqr*krfsqrn*krygrw*l
xxrsttrn*kircssyrpqpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcq
rrycrrrlkew*crygrinpissxvc*ixh*iptirsfrrqxxetxrrrrchqinrqesp
x*sr**ercccpikxexxx*yflinsix**kkkkkkk


Frame C:
GLMIPGMVVTFALAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDS
XYDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAK
EGTAAVVLKNGDAAMVELTPSRPXCVESFTEYPPLGRFAVXXXRQXVAVGVIKSTVKKAP
XKAGDKKGAAAPSKXX*xxnis**iafxnkkkkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB134 (SLB134Q) /CSM/SL/SLB1-B/SLB134Q.Seq.d/                       1158   0.0
VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                       1116   0.0
VFN472 (VFN472Q) /CSM/VF/VFN4-C/VFN472Q.Seq.d/                       1116   0.0
SLH395 (SLH395Q) /CSM/SL/SLH3-D/SLH395Q.Seq.d/                       1116   0.0
SLE701 (SLE701Q) /CSM/SL/SLE7-A/SLE701Q.Seq.d/                       1116   0.0
SLE555 (SLE555Q) /CSM/SL/SLE5-C/SLE555Q.Seq.d/                       1116   0.0
SLE403 (SLE403Q) /CSM/SL/SLE4-A/SLE403Q.Seq.d/                       1116   0.0
SLE193 (SLE193Q) /CSM/SL/SLE1-D/SLE193Q.Seq.d/                       1116   0.0
SLE138 (SLE138Q) /CSM/SL/SLE1-B/SLE138Q.Seq.d/                       1116   0.0
SLC291 (SLC291Q) /CSM/SL/SLC2-D/SLC291Q.Seq.d/                       1116   0.0
own update 2004. 5.31
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU060692) Dictyostelium discoideum slug cDNA, clone SLB134.     1158   0.0    1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...  1116   0.0    1
(AU284342) Dictyostelium discoideum gamete cDNA clone:FC-AN0...  1116   0.0    1
(AU263066) Dictyostelium discoideum vegetative cDNA clone:VS...  1116   0.0    1
(AU052273) Dictyostelium discoideum slug cDNA, clone SLA482.     1116   0.0    1
(AU052253) Dictyostelium discoideum slug cDNA, clone SLA434.     1116   0.0    1
(AU052252) Dictyostelium discoideum slug cDNA, clone SLA430.     1116   0.0    1
(AU052235) Dictyostelium discoideum slug cDNA, clone SLA290.     1116   0.0    1
(AU052232) Dictyostelium discoideum slug cDNA, clone SLA286.     1116   0.0    1
(AU052219) Dictyostelium discoideum slug cDNA, clone SLA265.     1116   0.0    1
dna update 2009. 1.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   327   1e-88
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   327   1e-88
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   327   1e-88
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   250   2e-65
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   249   4e-65
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   248   1e-64
AJ549292_1(AJ549292|pid:none) Podocoryne carnea mRNA for elongat...   247   2e-64
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   247   2e-64
AY643816_1(AY643816|pid:none) Lycogala epidendrum elongation fac...   247   2e-64
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   246   3e-64
protein update 2009. 7. 4
PSORT 

 psg: 0.96  gvh: 0.55  alm: 0.33  top: 0.33  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 24.0 %: extracellular, including cell wall
	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SLB134 is exc
5' end seq. ID SLB134F
5' end seq. 
>SLB134F.Seq
CTGGTTTAATGATACCAGGTATGGTCGTCACCTTTGCCCTAGCTGGTCTCTCAACTGAAG
TCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAG
GTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACT
CCNNNTACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACC
ATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTG
CCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCA
AAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCC
CATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCG
TCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCC
CAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATA
ATATTTCTTAATAAATAGCATTTTATAATAAAAAAAAA----------
Length of 5' end seq. 638
3' end seq. ID SLB134Z
3' end seq. 
>SLB134Z.Seq
----------ATCTCGTCCAAGGTGTGTTGAATCTTTCACTGAATACCCACCATTAGGTC
GTTTCGCCGTCAGGGTTTTGAGACAACCCGTCGCCGTCGGTGTCATCAAATCAACCGTCA
AGAAAGCCCCAGTTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAGGAATT
AATTAATTAATATTTCTTAATAAATAGCATTTTTTAATAAAAAAAAAAAAAAAAAAAAAA
AAA
Length of 3' end seq. 233
Connected seq. ID SLB134P
Connected seq. 
>SLB134P.Seq
CTGGTTTAATGATACCAGGTATGGTCGTCACCTTTGCCCTAGCTGGTCTCTCAACTGAAG
TCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAG
GTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACT
CCNNNTACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACC
ATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTG
CCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCA
AAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCC
CATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCG
TCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCC
CAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATA
ATATTTCTTAATAAATAGCATTTTATAATAAAAAAAAA----------ATCTCGTCCAAG
GTGTGTTGAATCTTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGGGTTTTGAG
ACAACCCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGTTAAAGCCGG
TGATAAGAAAGGTGCTGCTGCCCCATCAAAAAGGAATTAATTAATTAATATTTCTTAATA
AATAGCATTTTTTAATAAAAAAAAAAAAAAAAAAAAAAAAA
Length of connected seq. 871
Full length Seq ID SLB134E
Full length Seq. 
>SLB134E.Seq
CTGGTTTAATGATACCAGGTATGGTCGTCACCTTTGCCCTAGCTGGTCTCTCAACTGAAG
TCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAG
GTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACT
CCNNNTACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACC
ATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTG
CCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCA
AAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCC
CATCTCGTCCAANGTGTGTTGAATCNTTCACTGAATACCCACCATTAGGTCGTTTCGCCG
TCAGNGNTNTGAGACAANCCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCC
CAGNTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAANGAANTAANTAANTA
ATATTTCTTAATAAATAGCATTTTNTAATAAAAAAAAAAAAAAAAAAAAAAAAA
Length of full length seq. 654