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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB118
Library SL
(Link to library)
Clone ID SLB118
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLB118Z
(Link to Original site)
Representative DNA sequence 
>SLB118 (SLB118Q) /CSM/SL/SLB1-A/SLB118Q.Seq.d/
XXXXXXXXXXAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGA
CAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGC
TGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGT
CCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGC
TCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGT
CGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGA
ATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCG
TTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAA
GAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATA
AATAAATAATATTTCTTAATAAATAGCATTTTATAATAATTTT
sequence update 1998. 3.20
Translated Amino Acid sequence 
---SVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVL
NHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVEL
TPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*i
nnis**iafynnf
Translated Amino Acid sequence (All Frames) 
Frame A:
---nqskcitnnsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklss
stiqvksmlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn
*phlvqcvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk
*iiflnk*hfiii


Frame B:
---isrnasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrp
qpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygri
npissnvc*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkein
k*yflinsil**f


Frame C:
---SVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVL
NHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVEL
TPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*i
nnis**iafynnf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                       1136   0.0
SLE701 (SLE701Q) /CSM/SL/SLE7-A/SLE701Q.Seq.d/                       1136   0.0
SLE555 (SLE555Q) /CSM/SL/SLE5-C/SLE555Q.Seq.d/                       1136   0.0
SLE403 (SLE403Q) /CSM/SL/SLE4-A/SLE403Q.Seq.d/                       1136   0.0
SLE239 (SLE239Q) /CSM/SL/SLE2-B/SLE239Q.Seq.d/                       1136   0.0
SLE193 (SLE193Q) /CSM/SL/SLE1-D/SLE193Q.Seq.d/                       1136   0.0
SLE138 (SLE138Q) /CSM/SL/SLE1-B/SLE138Q.Seq.d/                       1136   0.0
SLC291 (SLC291Q) /CSM/SL/SLC2-D/SLC291Q.Seq.d/                       1136   0.0
SLC219 (SLC219Q) /CSM/SL/SLC2-A/SLC219Q.Seq.d/                       1136   0.0
SLC166 (SLC166Q) /CSM/SL/SLC1-C/SLC166Q.Seq.d/                       1136   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X55973) D. discoideum EF1-I gene for elongation factor 1 al...  1027   0.0    1
(AU285021) Dictyostelium discoideum gamete cDNA clone:FCL-AA...  1027   0.0    1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...  1027   0.0    1
(AU284420) Dictyostelium discoideum gamete cDNA clone:FC-AT2...  1027   0.0    1
(AU284342) Dictyostelium discoideum gamete cDNA clone:FC-AN0...  1027   0.0    1
(AU284225) Dictyostelium discoideum gamete cDNA clone:FC-AA2...  1027   0.0    1
(AU264497) Dictyostelium discoideum vegetative cDNA clone:VS...  1027   0.0    1
(AU263935) Dictyostelium discoideum vegetative cDNA clone:VS...  1027   0.0    1
(AU263821) Dictyostelium discoideum vegetative cDNA clone:VS...  1027   0.0    1
(AU263066) Dictyostelium discoideum vegetative cDNA clone:VS...  1027   0.0    1
dna update 2009. 1.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   315   4e-85
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   315   4e-85
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   315   4e-85
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   244   2e-63
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   242   6e-63
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   241   1e-62
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   241   1e-62
AB326305_1(AB326305|pid:none) Solea senegalensis SseEF1A4 mRNA f...   239   4e-62
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   239   4e-62
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   239   5e-62
protein update 2009. 5.31
PSORT 

 psg: 0.75  gvh: 0.29  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLB118 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLB118Z
3' end seq. 
>SLB118Z.Seq
----------AAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGA
CAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGC
TGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGT
CCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGC
TCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGT
CGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGA
ATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCG
TTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAA
GAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATA
AATAAATAATATTTCTTAATAAATAGCATTTTATAATAATTTT
Length of 3' end seq. 573
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -