>SLB115 (SLB115Q) /CSM/SL/SLB1-A/SLB115Q.Seq.d/
XXXXXXXXXXAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGAC
CAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGC
TGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGT
CCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGC
TCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGT
CGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGA
ATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCG
TTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAA
GAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATA
AATAAATAATATTTCTTAATAAATAGCATTTTATAATAAA

---QSKCITNNSQKPVQVTNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVL
NHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVEL
TPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*i
nnis**iafynk

Frame A:
---isrnasrttprspsr*pm*vstlktfqskklkevwslvtpktihhkklknsllklss
stiqvksmlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn
*phlvqcvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk
*iiflnk*hfii


Frame B:
---svemhheqlpearpgdqcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrp
qpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygri
npissnvc*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkein
k*yflinsil**


Frame C:
---QSKCITNNSQKPVQVTNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVL
NHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVEL
TPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*i
nnis**iafynk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB115 (SLB115Q) /CSM/SL/SLB1-A/SLB115Q.Seq.d/                       1130   0.0
SLH395 (SLH395Q) /CSM/SL/SLH3-D/SLH395Q.Seq.d/                       1114   0.0
SLB736 (SLB736Q) /CSM/SL/SLB7-B/SLB736Q.Seq.d/                       1114   0.0
SLB518 (SLB518Q) /CSM/SL/SLB5-A/SLB518Q.Seq.d/                       1114   0.0
SLB314 (SLB314Q) /CSM/SL/SLB3-A/SLB314Q.Seq.d/                       1114   0.0
SLB257 (SLB257Q) /CSM/SL/SLB2-C/SLB257Q.Seq.d/                       1114   0.0
SLB195 (SLB195Q) /CSM/SL/SLB1-D/SLB195Q.Seq.d/                       1114   0.0
SLB165 (SLB165Q) /CSM/SL/SLB1-C/SLB165Q.Seq.d/                       1114   0.0
SLA739 (SLA739Q) /CSM/SL/SLA7-B/SLA739Q.Seq.d/                       1114   0.0
VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                       1112   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU033510) Dictyostelium discoideum slug cDNA, clone SLB115.     1027   0.0    1
(X55973) D. discoideum EF1-I gene for elongation factor 1 al...   928   0.0    2
(AU033632) Dictyostelium discoideum slug cDNA, clone SLB249.      928   0.0    2
(AU052219) Dictyostelium discoideum slug cDNA, clone SLA265.      928   0.0    2
(AU033453) Dictyostelium discoideum slug cDNA, clone SLA844.      928   0.0    2
(BJ430284) Dictyostelium discoideum cDNA clone:ddv6o16, 3' e...   928   0.0    2
(BJ434165) Dictyostelium discoideum cDNA clone:ddv16g05, 3' ...   928   0.0    2
(BJ428296) Dictyostelium discoideum cDNA clone:ddv11m12, 3' ...   928   0.0    2
(BJ398777) Dictyostelium discoideum cDNA clone:dds2o04, 3' e...   928   0.0    2
(AU033412) Dictyostelium discoideum slug cDNA, clone SLA739.      928   0.0    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   280   1e-79
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   280   1e-79
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   280   1e-79
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   213   4e-58
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   214   2e-57
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   213   3e-57
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   211   3e-57
AB326305_1(AB326305|pid:none) Solea senegalensis SseEF1A4 mRNA f...   211   1e-56
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   207   1e-56
AY445082_1(AY445082|pid:none) Metarhizium anisopliae strain E6 t...   210   2e-56

 psg: 0.80  gvh: 0.13  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SLB115 is nuc

>SLB115Z.Seq
----------AATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGAC
CAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGC
TGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGT
CCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGC
TCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGT
CGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGA
ATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCG
TTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAA
GAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATA
AATAAATAATATTTCTTAATAAATAGCATTTTATAATAAA