>SLA321 (SLA321Q) /CSM/SL/SLA3-A/SLA321Q.Seq.d/
AAAGAAAGAAAACAATTGTAAATGAATTTACAAATGGACCACAATCAAAATTATCAAAAC
AACAAAGAAATATAGATTATTCAATTGATCATTCAATAAATAATAATAATAATAATAATA
ATAATGCTTTTAATAATTTTAATAATAATAATAATAATAATAATACAAATAATAGTAATG
TTTCAAGTATTGATAATAAAACAATTGATAAACAACAAACTATGATACTTCATTTACTTC
GTTTACTTTGTCAAAATGATTCAAATATTACAXXXXXXXXXX

RKKTIVNEFTNGPQSKLSKQQRNIDYSIDHSINNNNNNNNNAFNNFNNNNNNNNTNNSNV
SSIDNKTIDKQQTMILHLLRLLCQNDSNI---

Frame A:
kerkql*mnlqmdhnqnyqnnkei*iiqliiq*iiiiiiiimlliiliiiiiiiiqiivm
fqvliikqlinnkl*yfiyfvyfvkmiqil---


Frame B:
kkennck*iykwttikiikttkkyrlfn*sfnk********cf**f********yk***c
fky***nn**ttnydtsftsftlsk*fkyy---


Frame C:
RKKTIVNEFTNGPQSKLSKQQRNIDYSIDHSINNNNNNNNNAFNNFNNNNNNNNTNNSNV
SSIDNKTIDKQQTMILHLLRLLCQNDSNI---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLA321 (SLA321Q) /CSM/SL/SLA3-A/SLA321Q.Seq.d/                        194   5e-49
VHG755 (VHG755Q) /CSM/VH/VHG7-C/VHG755Q.Seq.d/                         40   0.017
SHK279 (SHK279Q) /CSM/SH/SHK2-D/SHK279Q.Seq.d/                         40   0.017
SHK261 (SHK261Q) /CSM/SH/SHK2-C/SHK261Q.Seq.d/                         40   0.017
SHH226 (SHH226Q) /CSM/SH/SHH2-B/SHH226Q.Seq.d/                         40   0.017
SHF878 (SHF878Q) /CSM/SH/SHF8-D/SHF878Q.Seq.d/                         40   0.017
SHE555 (SHE555Q) /CSM/SH/SHE5-C/SHE555Q.Seq.d/                         40   0.017
SHC765 (SHC765Q) /CSM/SH/SHC7-C/SHC765Q.Seq.d/                         40   0.017
SHB726 (SHB726Q) /CSM/SH/SHB7-B/SHB726Q.Seq.d/                         40   0.017
SHB453 (SHB453Q) /CSM/SH/SHB4-C/SHB453Q.Seq.d/                         40   0.017

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   194   4e-91  7
BX510660|BX510660.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-236A3.    38   0.005  5
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    38   0.006  3
AC135233|AC135233.19 Medicago truncatula clone mth2-14c14, complete sequence.    36   0.013  4
AC115609|AC115609.1 Dictyostelium discoideum chromosome 2 map 4874582-4895202 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    38   0.024  2
AC147491|AC147491.2 Otolemur garnettii clone CH256-355F7, WORKING DRAFT SEQUENCE, 3 ordered pieces.    34   0.068  5
BX537134|BX537134.5 Zebrafish DNA sequence from clone CH211-205J6.    38   0.072  5
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           34   0.092  17
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    30   0.13   10
AC079631|AC079631.16 Homo sapiens 12 BAC RP11-389G16 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    32   0.14   7

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 181,721,127

 psg: 0.64  gvh: 0.14  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SLA321 is nuc

>SLA321F.Seq
AAAGAAAGAAAACAATTGTAAATGAATTTACAAATGGACCACAATCAAAATTATCAAAAC
AACAAAGAAATATAGATTATTCAATTGATCATTCAATAAATAATAATAATAATAATAATA
ATAATGCTTTTAATAATTTTAATAATAATAATAATAATAATAATACAAATAATAGTAATG
TTTCAAGTATTGATAATAAAACAATTGATAAACAACAAACTATGATACTTCATTTACTTC
GTTTACTTTGTCAAAATGATTCAAATATTACA----------