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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ845
Library AF
(Link to library)
Clone ID AFJ845
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10892-1
Original site URL
Representative seq. ID AFJ845P
(Link to Original site)
Representative DNA sequence 
>AFJ845 (AFJ845Q) /CSM/AF/AFJ8-B/AFJ845Q.Seq.d/
CACTGTTGGCCTACTGGAAACTCTTGCACATATTAAGAAAGTTGTACATATAAAGTGATA
GTTAGTAGAATAGTAATAGTAACAGTGTAAAAGCTTTTTTTAAAAGAAAAAAAAAAATAA
TAAAAATAATAAATTTAAAACTTGTTTAAATAAAACTACAAGAAAAAATAAANCAAANCA
CCTGTTTAATATAAAGTAAAANGAAAAAAAAATXXXXXXXXXXCAAAAGTAAACCAAAAT
GGATCAGCACCTGAAAAATCATCATTATCATCACCAAGATCAAAGATATCAAATGCCAAA
TCAATCATTCCATTCTTAAGAGAAGAGGAGAGTAAAGTACTTGAATTTTGGCAAATTTGG
TCAGAATCAATTCCACCACAATCAGATTTACAAAGTGGAACAGCTATTGAATTCCATGTA
GCAACATCAATAGATATGCAAAAGTTAACATGGAGAACTGCAGGTCCACAAAATATTTTC
ATTCAAAAGATGGTAGATTTCTTCTGGAATGTAGGTGCGCCAGAAAGTGAAATCGATAGA
TTAAATGATGTAGGTGCAATCATTAACCCTGTAAAGATTGGTTCTTGGATTGATATGTCT
GATAAAGGTGGTATGGATGGTGATTGGTATTTCCCTGTTGATATTCCATTAAAATTAGCT
ATTGAAGCTTCTGATGCTGGCGAACCAACTCGTAAATTATCAGAATGGGCAGAATCAAAT
GAAGTTGTAAATTGTTATTCAATTGGTCGTGATATGGGTGCTGCACCACCAAGACAAACT
GAAACTAGATTTAAATTACCAGGTCCAGATTTTATAGCACAATTAAATTTGGCAGTTGAT
GCATTCAAAACCTTTGAATTCCCACCAATTCCATCAAATGCATTGGAAATTTTATATCAA
AGTTCAAATACTTTAGAGGCTGGTGGTTTATGTCTCAGTGTAATTACATCAAGTGAAGGT
TTCGTTAGATTAGGTCTTTTAATTCCAAAACCAACTCGTGATGTTGTTGGTCAACTTTGT
GAAATTGGTCAAGCTAACAGAGAAAGAATCTCCAAATTTGAAACAGCATTGGGTGGTTGT
GGTCCAGCATTTGTTGAATTCCAATATTTACAAAAAGGTTTTGGTTATACCGTCTATAAG
GAAGGTTTCGATATTGTTTTCCATTATATGGTTGGTGAGGATCAATCCGAATAAAGGTTT
CATCTTTGTCAATTTTCCTTTTTGATGTTTACAATTATCCCTTTTNACAAATATAAATAT
TCCCGCTTTCATCAATGTTNANAGNATCATCTCGAATTTCAAGATCTTTTCAAAATTT
sequence update 2003. 5. 2
Translated Amino Acid sequence 
hcwptgnscty*esctykvivsrivivtv*klflkekkk**k**i*nlfk*nykkk*xkx
pv*YKVKXKKN---

---KVNQNGSAPEKSSLSSPRSKISNAKSIIPFLREEESKVLEFWQIWSESIPPQSDLQS
GTAIEFHVATSIDMQKLTWRTAGPQNIFIQKMVDFFWNVGAPESEIDRLNDVGAIINPVK
IGSWIDMSDKGGMDGDWYFPVDIPLKLAIEASDAGEPTRKLSEWAESNEVVNCYSIGRDM
GAAPPRQTETRFKLPGPDFIAQLNLAVDAFKTFEFPPIPSNALEILYQSSNTLEAGGLCL
SVITSSEGFVRLGLLIPKPTRDVVGQLCEIGQANRERISKFETALGGCGPAFVEFQYLQK
GFGYTVYKEGFDIVFHYMVGEDQSE*rfhlcqfsflmftiipfxkykysrfhqcxxxhle
fqdlfki
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgnscty*esctykvivsrivivtv*klflkekkk**k**i*nlfk*nykkk*xkx
pv*YKVKXKKN---

---qk*tkmdqhlknhhyhhqdqryqmpnqsfhs*ekrrvkylnfgkfgqnqfhhnqiyk
veqllnsm*qhq*icks*hgelqvhkifsfkrw*issgm*vrqkvksid*mm*vqsltl*
rlvlgliclikvvwmvigisllifh*n*llkllmlanqlvnyqngqnqmkl*iviqlvvi
wvlhhqdklkldlnyqvqil*hn*iwqlmhskplnshqfhqmhwkfyikvqil*rlvvyv
sv*lhqvkvsld*vf*fqnqlvmllvnfvklvkltekespnlkqhwvvvvqhllnsniyk
kvlvipsirkvsilfsiiwlvrinpnkgfifvnfpf*clqlslxtninipafinvxxiis
nfkifskf

Frame B:
tvglletlahikkvvhik**lve****qcksff*kkkknnknnkfktclnkttrknkxxh
lfnik*xekk---

---kskpkwist*kiiiiitkikdikcqinhsilkrrge*st*ilanlvrinsttirftk
wnsy*ipcsninryakvnmencrstkyfhskdgrfllecrcark*nr*ik*crcnh*pck
dwfld*yv**rwygw*lvfpc*ysikisy*sf*cwrtns*iirmgrik*sckllfnws*y
gccttktn*n*i*itrsrfystikfgs*ciqnl*iptnsikcignfiskfkyfrgwwfms
qcnyik*rfr*irsfnsktns*ccwstl*nwss*qrknlqi*nsigwlwssic*ipiftk
rfwlyrl*grfrycfplygw*gsirikvsslsiflfdvynypfxqi*ifplssmxxxssr
isrsfqn

Frame C:
llaywkllhilrklyi*sds**nsnsnsvkaffkrkkkiikiinlklv*iklqekixqxt
cli*skxkkk---

---KVNQNGSAPEKSSLSSPRSKISNAKSIIPFLREEESKVLEFWQIWSESIPPQSDLQS
GTAIEFHVATSIDMQKLTWRTAGPQNIFIQKMVDFFWNVGAPESEIDRLNDVGAIINPVK
IGSWIDMSDKGGMDGDWYFPVDIPLKLAIEASDAGEPTRKLSEWAESNEVVNCYSIGRDM
GAAPPRQTETRFKLPGPDFIAQLNLAVDAFKTFEFPPIPSNALEILYQSSNTLEAGGLCL
SVITSSEGFVRLGLLIPKPTRDVVGQLCEIGQANRERISKFETALGGCGPAFVEFQYLQK
GFGYTVYKEGFDIVFHYMVGEDQSE*rfhlcqfsflmftiipfxkykysrfhqcxxxhle
fqdlfki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ845 (AFJ845Q) /CSM/AF/AFJ8-B/AFJ845Q.Seq.d/                       2147   0.0
AFG891 (AFG891Q) /CSM/AF/AFG8-D/AFG891Q.Seq.d/                       1441   0.0
CFH383 (CFH383Q) /CSM/CF/CFH3-D/CFH383Q.Seq.d/                       1340   0.0
AFL886 (AFL886Q) /CSM/AF/AFL8-D/AFL886Q.Seq.d/                       1306   0.0
SFJ839 (SFJ839Q) /CSM/SF/SFJ8-B/SFJ839Q.Seq.d/                       1265   0.0
AFL542 (AFL542Q) /CSM/AF/AFL5-B/AFL542Q.Seq.d/                       1257   0.0
AFI486 (AFI486Q) /CSM/AF/AFI4-D/AFI486Q.Seq.d/                       1229   0.0
CFA421 (CFA421Q) /CSM/CF/CFA4-A/CFA421Q.Seq.d/                       1213   0.0
SFJ828 (SFJ828Q) /CSM/SF/SFJ8-B/SFJ828Q.Seq.d/                       1156   0.0
SFE287 (SFE287Q) /CSM/SF/SFE2-D/SFE287Q.Seq.d/                       1146   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   955   0.0    17
BH162724|BH162724.1 ENTSF21TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68   9e-12  2
AZ688662|AZ688662.1 ENTJZ22TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68   9e-12  2
BH149924|BH149924.1 ENTPR18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68   9e-12  2
AZ682513|AZ682513.1 ENTLM69TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-07  2
AZ680089|AZ680089.1 ENTIB68TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-07  2
BH156423|BH156423.1 ENTRV68TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-07  2
AZ544479|AZ544479.1 ENTDG14TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-07  2
BH152801|BH152801.1 ENTPM43TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-07  2
BH153254|BH153254.1 ENTTH84TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44   4e-05  2
dna update 2004. 5.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_113(AC116986|pid:none) Dictyostelium discoideum chromos...   617   e-175
CP000250_1634(CP000250|pid:none) Rhodopseudomonas palustris HaA2...    39   0.52
CP000155_1238(CP000155|pid:none) Hahella chejuensis KCTC 2396, c...    37   1.5
A26480(A26480) knob protein - malaria parasite (Plasmodium falci...    36   2.6
A54495(A54495) knob protein precursor - malaria parasite (Plasmo...    36   2.6
(P13817) RecName: Full=Knob-associated histidine-rich protein;  ...    36   2.6
CU571250_2(CU571250|pid:none) Zebrafish DNA sequence from clone ...    34   9.7
protein update 2009. 3.28
PSORT 

 psg: 0.67  gvh: 0.41  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFJ845 is cyt
5' end seq. ID AFJ845F
5' end seq. 
>AFJ845F.Seq
CACTGTTGGCCTACTGGAAACTCTTGCACATATTAAGAAAGTTGTACATATAAAGTGATA
GTTAGTAGAATAGTAATAGTAACAGTGTAAAAGCTTTTTTTAAAAGAAAAAAAAAAATAA
TAAAAATAATAAATTTAAAACTTGTTTAAATAAAACTACAAGAAAAAATAAANCAAANCA
CCTGTTTAATATAAAGTAAAANGAAAAAAAAAT----------
Length of 5' end seq. 213
3' end seq. ID AFJ845Z
3' end seq. 
>AFJ845Z.Seq
NNNNNNNNNNCAAAAGTAAACCAAAATGGATCAGCACCTGAAAAATCATCATTATCATCA
CCAAGATCAAAGATATCAAATGCCAAATCAATCATTCCATTCTTAAGAGAAGAGGAGAGT
AAAGTACTTGAATTTTGGCAAATTTGGTCAGAATCAATTCCACCACAATCAGATTTACAA
AGTGGAACAGCTATTGAATTCCATGTAGCAACATCAATAGATATGCAAAAGTTAACATGG
AGAACTGCAGGTCCACAAAATATTTTCATTCAAAAGATGGTAGATTTCTTCTGGAATGTA
GGTGCGCCAGAAAGTGAAATCGATAGATTAAATGATGTAGGTGCAATCATTAACCCTGTA
AAGATTGGTTCTTGGATTGATATGTCTGATAAAGGTGGTATGGATGGTGATTGGTATTTC
CCTGTTGATATTCCATTAAAATTAGCTATTGAAGCTTCTGATGCTGGCGAACCAACTCGT
AAATTATCAGAATGGGCAGAATCAAATGAAGTTGTAAATTGTTATTCAATTGGTCGTGAT
ATGGGTGCTGCACCACCAAGACAAACTGAAACTAGATTTAAATTACCAGGTCCAGATTTT
ATAGCACAATTAAATTTGGCAGTTGATGCATTCAAAACCTTTGAATTCCCACCAATTCCA
TCAAATGCATTGGAAATTTTATATCAAAGTTCAAATACTTTAGAGGCTGGTGGTTTATGT
CTCAGTGTAATTACATCAAGTGAAGGTTTCGTTAGATTAGGTCTTTTAATTCCAAAACCA
ACTCGTGATGTTGTTGGTCAACTTTGTGAAATTGGTCAAGCTAACAGAGAAAGAATCTCC
AAATTTGAAACAGCATTGGGTGGTTGTGGTCCAGCATTTGTTGAATTCCAATATTTACAA
AAAGGTTTTGGTTATACCGTCTATAAGGAAGGTTTCGATATTGTTTTCCATTATATGGTT
GGTGAGGATCAATCCGAATAAAGGTTTCATCTTTGTCAATTTTCCTTTTTGATGTTTACA
ATTATCCCTTTTNACAAATATAAATATTCCCGCTTTCATCAATGTTNANAGNATCATCTC
GAATTTCAAGATCTTTTCAAAATTT
Length of 3' end seq. 1105
Connected seq. ID AFJ845P
Connected seq. 
>AFJ845P.Seq
CACTGTTGGCCTACTGGAAACTCTTGCACATATTAAGAAAGTTGTACATATAAAGTGATA
GTTAGTAGAATAGTAATAGTAACAGTGTAAAAGCTTTTTTTAAAAGAAAAAAAAAAATAA
TAAAAATAATAAATTTAAAACTTGTTTAAATAAAACTACAAGAAAAAATAAANCAAANCA
CCTGTTTAATATAAAGTAAAANGAAAAAAAAAT----------CAAAAGTAAACCAAAAT
GGATCAGCACCTGAAAAATCATCATTATCATCACCAAGATCAAAGATATCAAATGCCAAA
TCAATCATTCCATTCTTAAGAGAAGAGGAGAGTAAAGTACTTGAATTTTGGCAAATTTGG
TCAGAATCAATTCCACCACAATCAGATTTACAAAGTGGAACAGCTATTGAATTCCATGTA
GCAACATCAATAGATATGCAAAAGTTAACATGGAGAACTGCAGGTCCACAAAATATTTTC
ATTCAAAAGATGGTAGATTTCTTCTGGAATGTAGGTGCGCCAGAAAGTGAAATCGATAGA
TTAAATGATGTAGGTGCAATCATTAACCCTGTAAAGATTGGTTCTTGGATTGATATGTCT
GATAAAGGTGGTATGGATGGTGATTGGTATTTCCCTGTTGATATTCCATTAAAATTAGCT
ATTGAAGCTTCTGATGCTGGCGAACCAACTCGTAAATTATCAGAATGGGCAGAATCAAAT
GAAGTTGTAAATTGTTATTCAATTGGTCGTGATATGGGTGCTGCACCACCAAGACAAACT
GAAACTAGATTTAAATTACCAGGTCCAGATTTTATAGCACAATTAAATTTGGCAGTTGAT
GCATTCAAAACCTTTGAATTCCCACCAATTCCATCAAATGCATTGGAAATTTTATATCAA
AGTTCAAATACTTTAGAGGCTGGTGGTTTATGTCTCAGTGTAATTACATCAAGTGAAGGT
TTCGTTAGATTAGGTCTTTTAATTCCAAAACCAACTCGTGATGTTGTTGGTCAACTTTGT
GAAATTGGTCAAGCTAACAGAGAAAGAATCTCCAAATTTGAAACAGCATTGGGTGGTTGT
GGTCCAGCATTTGTTGAATTCCAATATTTACAAAAAGGTTTTGGTTATACCGTCTATAAG
GAAGGTTTCGATATTGTTTTCCATTATATGGTTGGTGAGGATCAATCCGAATAAAGGTTT
CATCTTTGTCAATTTTCCTTTTTGATGTTTACAATTATCCCTTTTNACAAATATAAATAT
TCCCGCTTTCATCAATGTTNANAGNATCATCTCGAATTTCAAGATCTTTTCAAAATTT
Length of connected seq. 1308
Full length Seq ID -
Full length Seq. -
Length of full length seq. -