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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLA259
Library SL
(Link to library)
Clone ID SLA259
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SLA259E
(Link to Original site)
Representative DNA sequence 
>SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/
AAATTGGATATATTTCATCATATGAACATCCAAAATTTGTTCAAAAATCAGATTTCTACA
AGACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAACCGATCAAGATCCACAAATGG
CAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTTTAGTGTTTGTAACTTACTATT
TAGCACATTATACCAGTAACAATTTTTATTTAAATTGTTTCTTGGCAATCGTTTACGCAC
TTTGCAACTCATTATTCTCAATGCATATGATGCATGATTCATGTCATGCTGCCATTTCAC
ATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTGATTTCGTCACTGGTGCTTCAT
TCCTTTCATGGTGTCATCAACATGTAATTGGTCATCATATTTACACCAATGTAAGAAATG
CCGATCCAGATCTTGGTCAAGGTGAGGTCGATTTCCGTATTGTCACTCCATTCCAAACTC
GTTCATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGGTATTTACACAC
TCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTC
GTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTG
TTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATT
TCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATG
TCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCA
ATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCC
TTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAA
TTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATA
ACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACC
TTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATT
AAATGAAATAACTTAAAAACCAATTATTTACTTTTGACAAACAGTAATATTAATAAATAC
AA
sequence update 1999. 6.24
Translated Amino Acid sequence 
IGYISSYEHPKFVQKSDFYKTLKERVRKHFKATDQDPQMAVSIFSRLALVYLLVFVTYYL
AHYTSNNFYLNCFLAIVYALCNSLFSMHMMHDSCHAAISHYPGVWKWMGASFDFVTGASF
LSWCHQHVIGHHIYTNVRNADPDLGQGEVDFRIVTPFQTRSWYHKYQHIYAPLLYGIYTL
KYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYHSFTDLICYF
LIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTTQDYGHGSLL
CTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTEAIMSHINYL
YKMGNDPDYVKKPLASKDD*mk*lknqlftfdkq*y**iq
Translated Amino Acid sequence (All Frames) 
Frame A:
kldifhhmniqnlfknqistrh*krelenilkqpikihkwqlvfshv*hwfif*cl*lti
*hiipvtifi*ivswqsfthfathysqci*cmihvmlpfhiiqaygngwahhlisslvlh
sfhgvinm*lviiftpm*empiqilvkvrsisvlslhsklvhgiinintftlhssmvfth
snivlkigkll*rmvkmvqfvlvspqisirpltslvncllfssvssfhsviialqi*fvi
ssllnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlkllkimvmvhs
fvpflvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnqtslkllchtlit
ftkwvmiqimlknh*pqkmik*nnlktnyllltnsninky


Frame B:
nwiyfii*tskicskirflqdikres*ktf*snrsrstngs*yfltfsiglsfsvcnllf
stlyq*qflfklflgnrlrtlqliilnayda*fmscchftlsrrmemdgrii*frhwcfi
pfmvsstcnwssylhqckkcrsrswsr*grfpychsipnsfmvs*isthlrstplwylht
qisysrlgsfckgw*kwcnsc*crhkfr*grlrhw*ivfcflpfhpstpls*lyrfnllf
phc*irlwlvshn*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*nysrlwswftp
lylf*wffkssscssfipincsrflpttctnckrsl*rt*hylph*tklh*syyvth*lp
lqng**srlc*ktislkr*lneit*kpiiyf*qtvilint


Frame C:
IGYISSYEHPKFVQKSDFYKTLKERVRKHFKATDQDPQMAVSIFSRLALVYLLVFVTYYL
AHYTSNNFYLNCFLAIVYALCNSLFSMHMMHDSCHAAISHYPGVWKWMGASFDFVTGASF
LSWCHQHVIGHHIYTNVRNADPDLGQGEVDFRIVTPFQTRSWYHKYQHIYAPLLYGIYTL
KYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYHSFTDLICYF
LIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTTQDYGHGSLL
CTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTEAIMSHINYL
YKMGNDPDYVKKPLASKDD*mk*lknqlftfdkq*y**iq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       2383   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       2383   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1471   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1350   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1346   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1340   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1326   0.0
VFD240 (VFD240Q) /CSM/VF/VFD2-B/VFD240Q.Seq.d/                       1324   0.0
VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       1324   0.0
CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                       1322   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB029311|AB029311.1 Dictyostelium discoideum FadA gene for fatty acid desaturase, complete cds.  1883   0.0    1
BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.  1471   0.0    1
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1471   0.0    1
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1471   0.0    1
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.    98   2e-95  11
AL589727|AL589727.4 Human DNA sequence from clone RP11-104N13 on chromosome 6.    34   0.030  8
BX511178|BX511178.6 Zebrafish DNA sequence from clone DKEY-221H15 in linkage group 20.    36   0.083  7
AX348716|AX348716.1 Sequence 174 from Patent WO0202807.            40   0.14   4
AX346129|AX346129.1 Sequence 1200 from Patent WO0200928.           40   0.14   4
AX281446|AX281446.1 Sequence 110 from Patent WO0177376.            40   0.14   4
dna update 2004. 1. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   769   0.0
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   533   e-150
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   470   e-131
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   263   7e-69
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   256   9e-67
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   253   1e-65
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   216   1e-54
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    214   4e-54
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   210   9e-53
AM949596_1(AM949596|pid:none) Mantoniella squamata d5 gene for A...   203   1e-50
protein update 2009. 7. 4
PSORT 

 psg: 0.63  gvh: 0.63  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for SLA259 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLA259Z
3' end seq. 
>SLA259Z.Seq
----------TACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTA
ACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCA
CCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCA
CCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCG
TTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATC
AAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATTTCAGCCCCAGTTGTTAAAGGTA
ATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCG
GTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAA
TTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTC
CTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTA
ACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAA
CAATAAAAATTTACATATTAAAATTTAAAAAAAAAAAA
Length of 3' end seq. 688
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID SLA259E
Full length Seq. 
>SLA259E.Seq
AAATTGGATATATTTCATCATATGAACATCCAAAATTTGTTCAAAAATCAGATTTCTACA
AGACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAACCGATCAAGATCCACAAATGG
CAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTTTAGTGTTTGTAACTTACTATT
TAGCACATTATACCAGTAACAATTTTTATTTAAATTGTTTCTTGGCAATCGTTTACGCAC
TTTGCAACTCATTATTCTCAATGCATATGATGCATGATTCATGTCATGCTGCCATTTCAC
ATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTGATTTCGTCACTGGTGCTTCAT
TCCTTTCATGGTGTCATCAACATGTAATTGGTCATCATATTTACACCAATGTAAGAAATG
CCGATCCAGATCTTGGTCAAGGTGAGGTCGATTTCCGTATTGTCACTCCATTCCAAACTC
GTTCATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGGTATTTACACAC
TCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTC
GTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTG
TTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATT
TCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATG
TCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCA
ATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCC
TTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAA
TTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATA
ACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACC
TTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGATGATT
AAATGAAATAACTTAAAAACCAATTATTTACTTTTGACAAACAGTAATATTAATAAATAC
AA
Length of full length seq. 1202