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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLA256
Library SL
(Link to library)
Clone ID SLA256
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SLA256Z
(Link to Original site)
Representative DNA sequence 
>SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/
XXXXXXXXXXCGTATGTCACTCCATTCCAAACTCGTTCATGGTATCATAAATATCAACAC
ATTTACGCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAA
GCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGAT
AAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTC
CGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGT
TGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCT
ACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTT
AAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTA
AATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTT
GTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAAC
TTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGAT
TATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAATAACTTAAAAACCAATTA
TTTACTTTTGACAAACAGTAATATTAATAAATACAA
sequence update 1997. 6. 5
Translated Amino Acid sequence 
---YVTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDK
AAYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFAT
PERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLV
PIVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKPLASKDD*mk*lknqlf
tfdkq*y**iq
Translated Amino Acid sequence (All Frames) 
Frame A:
---vchsipnsfmvs*isthlrstplwylhtqisysrlgsfckgw*kwcnsc*crhkfr*
grlrhw*ivfcflpfhpstpls*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcy
p*ktr*tisnq*rlgnpst*nysrlwswftplylf*wffkssscssfipincsrflpttc
tnckrsl*rt*hylph*tklh*syyvth*lplqng**srlc*ktislkr*lneit*kpii
yf*qtvilint


Frame B:
---YVTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDK
AAYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFAT
PERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLV
PIVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKPLASKDD*mk*lknqlf
tfdkq*y**iq


Frame C:
---mslhsklvhgiinintftlhssmvfthsnivlkigkll*rmvkmvqfvlvspqisir
pltslvncllfssvssfhsviialqi*fvissllnsslvgisqlisklvmslkisnsllp
lkdqmnhlksmkigqsfnlkllkimvmvhsfvpflvvl*iiklfiiyshqllkisthnly
ql*kkfvknitllttlnqtslkllchtlitftkwvmiqimlknh*pqkmik*nnlktnyl
lltnsninky
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1479   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1471   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1471   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1350   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1346   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1338   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1326   0.0
VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       1322   0.0
CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                       1322   0.0
SLE621 (SLE621Q) /CSM/SL/SLE6-A/SLE621Q.Seq.d/                       1320   0.0
own update 2004. 8.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of longfatty acids in plants.  1479   0.0    1
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1479   0.0    1
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1479   0.0    1
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   971   0.0    2
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.    90   1e-54  7
AE017155|AE017155.1 Haemophilus ducreyi strain 35000HP section 5 of 6 of the complete genome.    34   0.081  12
AL591476|AL591476.7 Zebrafish DNA sequence from clone XX-263J20 on chromosome 7.    36   0.17   7
AP001511|AP001511.1 Bacillus halodurans genomic DNA, section 5/14.    40   0.65   6
BZ067998|BZ067998.1 lki76e10.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46   0.71   1
BZ033449|BZ033449.1 oej70g10.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46   0.71   1
dna update 2004. 1. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   478   e-134
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   325   9e-88
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   267   2e-70
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   170   5e-41
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   162   1e-38
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   157   3e-37
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   156   6e-37
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   146   8e-34
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   145   1e-33
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    142   1e-32
protein update 2009. 5.31
PSORT 

 psg: 0.57  gvh: 0.32  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for SLA256 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLA256Z
3' end seq. 
>SLA256Z.Seq
----------CGTATGTCACTCCATTCCAAACTCGTTCATGGTATCATAAATATCAACAC
ATTTACGCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAA
GCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGAT
AAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTC
CGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGT
TGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCT
ACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTT
AAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTA
AATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTT
GTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAAC
TTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGAT
TATGTTAAAAAACCATTAGCCTCAAAAGATGATTAAATGAAATAACTTAAAAACCAATTA
TTTACTTTTGACAAACAGTAATATTAATAAATACAA
Length of 3' end seq. 746
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -