>SLA253 (SLA253Q) /CSM/SL/SLA2-C/SLA253Q.Seq.d/
XXXXXXXXXXTCCAACTGTTAATATGGGTGATTGCCACAGATACAATGTTGATTTAGTAT
TCAAGAAAGATAAATCTGGTAATGTTATGCCAAAGAAAGAGTTAAGAGAAAGTGCATACG
TTCCACATGGACCAATTGACCCAGCCACTTGGAAATATTATACTTTTGTTCAAGGTAAAT
GGACTGGTTTTGGTTGTGACCCTCAAAATGTCGTCTTTTCCGGTGCTGAAGGTGGTATGC
CATTACAACTCGGTTATGGTGCAAATGGTAAAAATGGTGATAATGGTATCTCTGTTTGGT
TAATTTATGGTTACACTATTGTTGATATCAATTGTAACATTCGTCCAATCATTACAGCTG
CAACCATAGCTACAACCACAGCTGCAACTACAACTGCAACTACAACCGCAACCACAGCTA
CAACCACAGCTACAACCACAGCTACCTCTTAAATTTAATCAAAAAACATTTTTTATCAAG
TATCACTACAATAATAATTTTAATAATAATAGTATCAAAAAATGATATTAAAAATCCCCT
TTTTTTCTCTATAATTTTTAAATTAAATATGAAATAAATAAAAAAAAAAAAAAAATCTCC
A

---PTVNMGDCHRYNVDLVFKKDKSGNVMPKKELRESAYVPHGPIDPATWKYYTFVQGKW
TGFGCDPQNVVFSGAEGGMPLQLGYGANGKNGDNGISVWLIYGYTIVDINCNIRPIITAA
TIATTTAATTTATTTATTATTTATTTATS*i*sknifyqvslq**f****yqkmilkipf
fsl*fln*i*nk*kkkknl

Frame A:
---PTVNMGDCHRYNVDLVFKKDKSGNVMPKKELRESAYVPHGPIDPATWKYYTFVQGKW
TGFGCDPQNVVFSGAEGGMPLQLGYGANGKNGDNGISVWLIYGYTIVDINCNIRPIITAA
TIATTTAATTTATTTATTATTTATTTATS*i*sknifyqvslq**f****yqkmilkipf
fsl*fln*i*nk*kkkknl


Frame B:
---qlliwviatdtmli*ysrkinlvmlcqrks*ekvhtfhmdqltqplgniillfkvng
lvlvvtlkmssfpvlkvvchynsvmvqmvkmvimvslfg*fmvtlllisivtfvqslqlq
p*lqpqlqlqlqlqpqpqlqpqlqpqlplkfnqktffikyhynnnfnnnsikk*y*kspf
flynf*ikyeinkkkkkis


Frame C:
---nc*yg*lpqiqc*fsiqer*iw*cyakervkrkcirstwtn*pshleilyfcsr*md
wfwl*pskcrlfrc*rwyaittrlwckw*kw**wylclvnlwlhyc*yql*hssnhyscn
hsynhscnyncnynrnhsynhsynhsyllnlikkhflssittiiiliiivskndiknplf
fsiifklnmk*ikkkkksp

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLA253 (SLA253Q) /CSM/SL/SLA2-C/SLA253Q.Seq.d/                       1086   0.0
SSB820 (SSB820Q) /CSM/SS/SSB8-A/SSB820Q.Seq.d/                        398   e-110
SLK165 (SLK165Q) /CSM/SL/SLK1-C/SLK165Q.Seq.d/                        398   e-110
SLJ763 (SLJ763Q) /CSM/SL/SLJ7-C/SLJ763Q.Seq.d/                        398   e-110
SLI565 (SLI565Q) /CSM/SL/SLI5-C/SLI565Q.Seq.d/                        398   e-110
SLI557 (SLI557Q) /CSM/SL/SLI5-C/SLI557Q.Seq.d/                        398   e-110
SLI431 (SLI431Q) /CSM/SL/SLI4-B/SLI431Q.Seq.d/                        398   e-110
SLI364 (SLI364Q) /CSM/SL/SLI3-C/SLI364Q.Seq.d/                        398   e-110
SLI141 (SLI141Q) /CSM/SL/SLI1-B/SLI141Q.Seq.d/                        398   e-110
SLH738 (SLH738Q) /CSM/SL/SLH7-B/SLH738Q.Seq.d/                        398   e-110

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.   678   0.0    6
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].   678   0.0    1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.   678   0.0    1
X16491|X16491.1 Dictyostelium discoideum sp96 gene for spore coat protein SP96.   353   e-104  2
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    54   3e-13  9
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    72   1e-08  1
AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    72   1e-08  1
AC122076|AC122076.4 Rattus norvegicus clone CH230-277E22, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    72   1e-08  2
AL928941|AL928941.12 Zebrafish DNA sequence from clone CH211-236L14 in linkage group 25.    50   2e-07  7
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            42   2e-07  11

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...   248   1e-64
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...   248   1e-64
AM942444_1000(AM942444|pid:none) Corynebacterium urealyticum DSM...    34   2.6
CR382132_389(CR382132|pid:none) Yarrowia lipolytica strain CLIB1...    33   4.4
CP001097_1660(CP001097|pid:none) Chlorobium limicola DSM 245, co...    33   4.4
(Q39CQ5) RecName: Full=Protoheme IX farnesyltransferase;        ...    33   7.5
(B1YN86) RecName: Full=Protoheme IX farnesyltransferase;        ...    33   7.5
(A1UZV8) RecName: Full=Protoheme IX farnesyltransferase;        ...    33   7.5
(Q0BBM3) RecName: Full=Protoheme IX farnesyltransferase;        ...    33   7.5
(A3N5D1) RecName: Full=Protoheme IX farnesyltransferase;        ...    33   7.5

 psg: 0.63  gvh: 0.22  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for SLA253 is nuc

>SLA253Z.Seq
----------TCCAACTGTTAATATGGGTGATTGCCACAGATACAATGTTGATTTAGTAT
TCAAGAAAGATAAATCTGGTAATGTTATGCCAAAGAAAGAGTTAAGAGAAAGTGCATACG
TTCCACATGGACCAATTGACCCAGCCACTTGGAAATATTATACTTTTGTTCAAGGTAAAT
GGACTGGTTTTGGTTGTGACCCTCAAAATGTCGTCTTTTCCGGTGCTGAAGGTGGTATGC
CATTACAACTCGGTTATGGTGCAAATGGTAAAAATGGTGATAATGGTATCTCTGTTTGGT
TAATTTATGGTTACACTATTGTTGATATCAATTGTAACATTCGTCCAATCATTACAGCTG
CAACCATAGCTACAACCACAGCTGCAACTACAACTGCAACTACAACCGCAACCACAGCTA
CAACCACAGCTACAACCACAGCTACCTCTTAAATTTAATCAAAAAACATTTTTTATCAAG
TATCACTACAATAATAATTTTAATAATAATAGTATCAAAAAATGATATTAAAAATCCCCT
TTTTTTCTCTATAATTTTTAAATTAAATATGAAATAAATAAAAAAAAAAAAAAAATCTCC
A