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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLA160
Library SL
(Link to library)
Clone ID SLA160
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16453-1
Original site URL
Representative seq. ID SLA160Z
(Link to Original site)
Representative DNA sequence 
>SLA160 (SLA160Q) /CSM/SL/SLA1-C/SLA160Q.Seq.d/
XXXXXXXXXXGTATCACAAGCAAATTCATGCAAAACCCAGTCAGTATCCTCGTCAAAAAG
GATGAATTAACCCTTGAAGGTATTAGACAATTCTACGTCAACGTTGGTGTTGAACAAGGT
AAATTTGAAGTTTTAACCGATCTCTACGAAACCCTCTCTATCACTCAATCCGTTATTTTC
TGTAACACCCGTAGAAAGGTCGATTGGTTAACAAGCAAAATGACCGAACAAAAATTCACC
GTCTCATCAACTCACGGTGACCAAAAGGATCGTGATGGTATCTTACAAGCTTTCCGTTCT
GGTGCTACTCGTGTCCTCATCACCACCGATCTCTTAGCTCGTGGTATTGATGTCCAACAA
GTATCACTCGTTATCAACTTTGATTTACCAACCAACATTGAAAATTACATTCATCGTATC
GGTCGTTCAGGTCGTTTCGGTCGTAAGGGTGTTGCCATCAACTTTATCACTGACGCTGAA
AGAGGTCAAATCGATGAATTATCTCGTCATTACAATACCTACATTGCCGAAATGCCACAA
GACATTGGAAGTTTCCTCTAAACGAGTTCCAAAAATGGTTTATAGTTCAGTTGGTCCCCA
TTTGGTGAGGGCCATAGGGTTTATAAATCGACCGCCCCCCTCCCTATCCAATACTCACAC
CACAACAACATATTTCATCCGCTCATTATAAAAACAACAAAAACTTCAACTCTTTTACAC
CTCGTTTAAT
sequence update 1997. 5.20
Translated Amino Acid sequence 
---ITSKFMQNPVSILVKKDELTLEGIRQFYVNVGVEQGKFEVLTDLYETLSITQSVIFC
NTRRKVDWLTSKMTEQKFTVSSTHGDQKDRDGILQAFRSGATRVLITTDLLARGIDVQQV
SLVINFDLPTNIENYIHRIGRSGRFGRKGVAINFITDAERGQIDELSRHYNTYIAEMPQD
IGSFL*tsskngl*fswspfgeghrvykstaplpiqyshhnnifhpliikttktstllhl
v*
Translated Amino Acid sequence (All Frames) 
Frame A:
---yhkqihakpsqyprqkg*inp*ry*tilrqrwc*tr*i*sfnrslrnplyhsiryfl
*hp*kgrlvnkqndrtkihrlinsr*pkgs*wyltsfpfwcyscphhhrslsswy*cpts
itryql*ftnqh*klhssyrsfrsfrs*gcchqlyh*r*krsnr*iisslqylhcrnatr
hwkfplnefqkwfivqlvpiw*gp*gl*idrpppypiltpqqhissahyknnknfnsftp
rl


Frame B:
---ITSKFMQNPVSILVKKDELTLEGIRQFYVNVGVEQGKFEVLTDLYETLSITQSVIFC
NTRRKVDWLTSKMTEQKFTVSSTHGDQKDRDGILQAFRSGATRVLITTDLLARGIDVQQV
SLVINFDLPTNIENYIHRIGRSGRFGRKGVAINFITDAERGQIDELSRHYNTYIAEMPQD
IGSFL*tsskngl*fswspfgeghrvykstaplpiqyshhnnifhpliikttktstllhl
v*


Frame C:
---sqanscktqsvssskrmn*plkvldnststlvlnkvnlkf*pistkpslslnplfsv
tpversig*qak*pnknspshqltvtkrivmvsyklsvlvllvsssppis*lvvlmsnky
hslstliyqptlkitfivsvvqvvsvvrvlpstlsltlkevksmnylvitiptlpkchkt
levsskrvpkmvyssvgphlvraigfinrpppslsnthttttyfirsl*kqqklqlfyts
fn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLA160 (SLA160Q) /CSM/SL/SLA1-C/SLA160Q.Seq.d/                       1427   0.0
CHC333 (CHC333Q) /CSM/CH/CHC3-B/CHC333Q.Seq.d/                       1419   0.0
VFI417 (VFI417Q) /CSM/VF/VFI4-A/VFI417Q.Seq.d/                       1415   0.0
VFB309 (VFB309Q) /CSM/VF/VFB3-A/VFB309Q.Seq.d/                       1415   0.0
SFK695 (SFK695Q) /CSM/SF/SFK6-D/SFK695Q.Seq.d/                       1415   0.0
SFI627 (SFI627Q) /CSM/SF/SFI6-B/SFI627Q.Seq.d/                       1415   0.0
SFC409 (SFC409Q) /CSM/SF/SFC4-A/SFC409Q.Seq.d/                       1415   0.0
CFH334 (CFH334Q) /CSM/CF/CFH3-B/CFH334Q.Seq.d/                       1415   0.0
CFG694 (CFG694Q) /CSM/CF/CFG6-D/CFG694Q.Seq.d/                       1415   0.0
CFC512 (CFC512Q) /CSM/CF/CFC5-A/CFC512Q.Seq.d/                       1415   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U78759|U78759.1 Dictyostelium discoideum IfdA (ifdA) mRNA, partial cds.  1400   0.0    1
CF245450|CF245450.1 Fcylcold195 Fragilariopsis cylindrus SMART cDNA library (Clontech) Fragilariopsis cylindrus cDNA clone Antarctic 5', mRNA sequence.   113   1e-38  3
X80796|X80796.1 S.pombe eIF-4A mRNA for translation initiation factor.   115   1e-36  2
L40627|L40627.1 Schizosaccharomyces pombe cell cycle control protein eIF-4A (tif1) mRNA, complete cds.   115   1e-36  2
AL132828|AL132828.1 S.pombe chromosome I cosmid c1006.            115   2e-35  2
CB403814|CB403814.1 OSTR012H9_1 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.   117   6e-33  3
Z12116|Z12116.1 C.elegans mRNA for eIF-4A homologue.              117   5e-32  3
CB028184|CB028184.1 TgESTzyd49b10.y1 TgRH Tachyzoite Norm 7 cDNA Library Toxoplasma gondii cDNA clone TgESTzyd49b10.y1 5' similar to TR:O02494 O02494 TRANSLATION INITIATION FACTOR. ;, mRNA sequence.    80   8e-30  4
CB025494|CB025494.1 TgESTzyc78c04.y1 TgRH Tachyzoite Norm 5 cDNA Library Toxoplasma gondii cDNA clone TgESTzyc78c04.y1 5' similar to TR:O02494 O02494 TRANSLATION INITIATION FACTOR. ;, mRNA sequence.    80   2e-29  4
AJ320155|AJ320155.1 Toxoplasma gondii mRNA for eukaryotic translation initiation factor  4A (toif4A gene).    80   5e-28  4
dna update 2004. 1. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U78759_1(U78759|pid:none) Dictyostelium discoideum IfdA (ifdA) m...   360   2e-98
DQ667140_1(DQ667140|pid:none) Callinectes sapidus eukaryotic ini...   255   8e-67
AJ320155_1(AJ320155|pid:none) Toxoplasma gondii mRNA for eukaryo...   255   8e-67
(A5DVM3) RecName: Full=ATP-dependent RNA helicase eIF4A;        ...   253   4e-66
(P27639) RecName: Full=Eukaryotic initiation factor 4A;         ...   251   2e-65
CP001142_274(CP001142|pid:none) Phaeodactylum tricornutum CCAP 1...   249   6e-65
BC049427_1(BC049427|pid:none) Danio rerio eukaryotic translation...   248   1e-64
(Q5KN60) RecName: Full=ATP-dependent RNA helicase eIF4A;        ...   248   1e-64
BC048899_1(BC048899|pid:none) Danio rerio eukaryotic translation...   248   1e-64
(A7TK55) RecName: Full=ATP-dependent RNA helicase eIF4A;        ...   248   1e-64
protein update 2009. 5.31
PSORT 

 psg: 0.80  gvh: 0.50  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for SLA160 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLA160Z
3' end seq. 
>SLA160Z.Seq
----------GTATCACAAGCAAATTCATGCAAAACCCAGTCAGTATCCTCGTCAAAAAG
GATGAATTAACCCTTGAAGGTATTAGACAATTCTACGTCAACGTTGGTGTTGAACAAGGT
AAATTTGAAGTTTTAACCGATCTCTACGAAACCCTCTCTATCACTCAATCCGTTATTTTC
TGTAACACCCGTAGAAAGGTCGATTGGTTAACAAGCAAAATGACCGAACAAAAATTCACC
GTCTCATCAACTCACGGTGACCAAAAGGATCGTGATGGTATCTTACAAGCTTTCCGTTCT
GGTGCTACTCGTGTCCTCATCACCACCGATCTCTTAGCTCGTGGTATTGATGTCCAACAA
GTATCACTCGTTATCAACTTTGATTTACCAACCAACATTGAAAATTACATTCATCGTATC
GGTCGTTCAGGTCGTTTCGGTCGTAAGGGTGTTGCCATCAACTTTATCACTGACGCTGAA
AGAGGTCAAATCGATGAATTATCTCGTCATTACAATACCTACATTGCCGAAATGCCACAA
GACATTGGAAGTTTCCTCTAAACGAGTTCCAAAAATGGTTTATAGTTCAGTTGGTCCCCA
TTTGGTGAGGGCCATAGGGTTTATAAATCGACCGCCCCCCTCCCTATCCAATACTCACAC
CACAACAACATATTTCATCCGCTCATTATAAAAACAACAAAAACTTCAACTCTTTTACAC
CTCGTTTAAT
Length of 3' end seq. 720
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -