>SLA124 (SLA124Q) /CSM/SL/SLA1-A/SLA124Q.Seq.d/
GTTGGATTTGAAGATCATATCGCAGCTATCACAAGAAACTATCAAGTTAACCCACGTTTA
GATTATAGAACTGTTACAGCAGTAAATGGTCCACTCGTTATTTTAGATAATATCAGAGGT
CCAAAATTCTCTGAAATTGTAACTTTAACTTTAGGTGATGGTACTCAAAGAAAAGGTCAA
GTTTTGGAAATCCAAGGTAAAAGAGCTGTTGTACAAGTTTTCGAAGGTACAATTGGTATT
GATGCCAAACACACTCGTTGTGAATTCTCTGGTGATATTTTAAAGATGCCAGTATCAGAA
GATTCATTAGGTCGTATTTTCAATGGTTCAGGTAAACCAGTTGATAAAGGTCCAAATGTT
TTAGCTGAAGAATACTTAGATATTCAAGGTCAACCAATCAATCCATCAGTTAGAGTTTAC
CCACAAGAAATGATTCAAACTGGTATCTCTGCTATTGATACTATGAATTCAATTGCTCGT
GGTCAAAAGATTCCAATTTTCTCTGCTGCAGGTTTACCACACAATGAAATTGCCGCTCAA
ATTTGTCGTCAAGCTGGTTTAGTTAAAAAATCAGGTAAAGGTGTTATTGATGATCATGAA
GATAATTTTGCTATTGTATTCGCAGCTATGGGTGTTAATATGGAAXXXXXXXXXXCCTTG
GNGGGGGNGCCCTTTNTTCTGGGGGTTACCCCNCCTTGGGNTTTTTTGGCCGTTTCGGAT
NTNGCTTTNTCGGTCNAACCCCTTTTGGAAATCGGGGTTTCTTTTATTCCTTGGGTCTTG
GNTGGNCTTTTTTTGGGNCTTTCCCCGGCNTTNNCCCCCANCGTTTCCCTGGAAAANCTT
TTTACCCATTCTTTTCCCGTTCCTCCAGGGGTTCTTTNGGTTCCCCTTNCTTATTTTTTA
AAGGGAAAAANTTTTAATCCAAAAAAAAAAANTTAAATTAAATTTTAAAATTAAATTAAA
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AA

VGFEDHIAAITRNYQVNPRLDYRTVTAVNGPLVILDNIRGPKFSEIVTLTLGDGTQRKGQ
VLEIQGKRAVVQVFEGTIGIDAKHTRCEFSGDILKMPVSEDSLGRIFNGSGKPVDKGPNV
LAEEYLDIQGQPINPSVRVYPQEMIQTGISAIDTMNSIARGQKIPIFSAAGLPHNEIAAQ
ICRQAGLVKKSGKGVIDDHEDNFAIVFAAMGVNME---

---LXGXPFXLGVTPPWXFLAVSDXAXSVXPLLEIGVSFIPWVLXGLFLGLSPAXXPXVS
LEXLFTHSFPVPPGVLXVPLXYFLKGKXFNPKKKX*ikf*n*iklkkkkkkkkkkkkkkk
kkkk

Frame A:
VGFEDHIAAITRNYQVNPRLDYRTVTAVNGPLVILDNIRGPKFSEIVTLTLGDGTQRKGQ
VLEIQGKRAVVQVFEGTIGIDAKHTRCEFSGDILKMPVSEDSLGRIFNGSGKPVDKGPNV
LAEEYLDIQGQPINPSVRVYPQEMIQTGISAIDTMNSIARGQKIPIFSAAGLPHNEIAAQ
ICRQAGLVKKSGKGVIDDHEDNFAIVFAAMGVNME---

---pwxgxpxfwglpxlgxfwpfrxxlxrsnpfwksgfllflgswxxffwxfprxxppxf
pwkxflpilfpflqgffxfpxlif*rekxliqkkkxklnfkikln*kkkkkkkkkkkkkk
kkkkk

Frame B:
ldlkiisqlsqetiklthv*iiellqq*mvhslf*iisevqnslkl*l*l*vmvlkekvk
fwkskvkellykfskvqlvlmpntlvvnslvif*rcqyqkih*vvfsmvqvnqlikvqmf
*lknt*ifkvnqsihqlefthkk*fklvslllil*iqllvvkrfqfsllqvyhtmklplk
fvvklv*lknqvkvllmimkiilllysqlwvliw---

---lgggalxsggypxlgffgrfgxxfxgxtpfgnrgffyslglgwxffgxfpgxxpxrf
pgkxfypffsrssrgsxgspxlffkgkxf*skkkxln*ilkln*ikkkkkkkkkkkkkkk
kkkkk

Frame C:
wi*rsyrsyhkklss*ptfrl*ncysskwstryfr*yqrskil*ncnfnfr*wyskkrss
fgnpr*kscctsfrrynwy*cqthsl*ilw*yfkdasirrfirsyfqwfr*ts**rskcf
s*rilrysrstnqsis*slptrndsnwylcy*yyefncswskdsnflccrfttq*ncrsn
lssswfs*kir*rcy**s*r*fcycirsygc*yg---

---LXGXPFXLGVTPPWXFLAVSDXAXSVXPLLEIGVSFIPWVLXGLFLGLSPAXXPXVS
LEXLFTHSFPVPPGVLXVPLXYFLKGKXFNPKKKX*ikf*n*iklkkkkkkkkkkkkkkk
kkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLA124 (SLA124Q) /CSM/SL/SLA1-A/SLA124Q.Seq.d/                       1279   0.0
AFO705 (AFO705Q) /CSM/AF/AFO7-A/AFO705Q.Seq.d/                       1247   0.0
AFH533 (AFH533Q) /CSM/AF/AFH5-B/AFH533Q.Seq.d/                       1239   0.0
CFD847 (CFD847Q) /CSM/CF/CFD8-B/CFD847Q.Seq.d/                       1215   0.0
CFF864 (CFF864Q) /CSM/CF/CFF8-C/CFF864Q.Seq.d/                       1207   0.0
AFF375 (AFF375Q) /CSM/AF/AFF3-D/AFF375Q.Seq.d/                       1197   0.0
AFB239 (AFB239Q) /CSM/AF/AFB2-B/AFB239Q.Seq.d/                       1187   0.0
AFM386 (AFM386Q) /CSM/AF/AFM3-D/AFM386Q.Seq.d/                       1148   0.0
VFI477 (VFI477Q) /CSM/VF/VFI4-D/VFI477Q.Seq.d/                       1144   0.0
AFC267 (AFC267Q) /CSM/AF/AFC2-C/AFC267Q.Seq.d/                       1138   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.   618   0.0    8
CN582638|CN582638.1 USDA-FP_125701 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP020_G09, mRNA sequence.   202   1e-49  2
CN758647|CN758647.1 ID0AAA22DB11RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA22DB11 5', mRNA sequence.   202   2e-49  2
CF588057|CF588057.1 USDA-FP_121200-058 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP-009_F06 5', mRNA sequence.   194   3e-47  2
CN586814|CN586814.1 USDA-FP_129886 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP090_B10, mRNA sequence.   194   3e-47  2
CN764382|CN764382.1 ID0AAA9DA11RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA9DA11 5', mRNA sequence.   194   4e-47  2
BE581425|BE581425.1 kq45f06.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to gb:L35249 VACUOLAR ATP SYNTHASE SUBUNIT B, BRAIN ISOFORM (HUMAN);, mRNA sequence.    64   2e-42  5
X54875|X54875.1 C. tropicalis gene for vacuolar ATPase subunit b.   155   3e-42  3
BI509187|BI509187.1 BB170005B20H04 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170005B20H04 5', mRNA sequence.   155   5e-42  3
CD523888|CD523888.1 kw18c01.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:VATB_CAEEL Q19626 PROBABLE VACUOLAR ATP SYNTHASE SUBUNIT B ;, mRNA sequence.    80   4e-39  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NU1) RecName: Full=V-type proton ATPase subunit B;          ...   428   e-118
AY641405_1(AY641405|pid:none) Entamoeba histolytica vacuolar ATP...   354   3e-96
AF189779_1(AF189779|pid:none) Carcinus maenas vacuolar ATP synth...   335   1e-90
EU727173_1(EU727173|pid:none) Bombyx mori strain NB vacuolar ATP...   335   2e-90
(P31410) RecName: Full=V-type proton ATPase subunit B;          ...   333   4e-90
AF037468_1(AF037468|pid:none) Culex pipiens quinquefasciatus B s...   332   9e-90
AY197611_1(AY197611|pid:none) Artemia franciscana vacuolar proto...   331   2e-89
BC062202_1(BC062202|pid:none) Mus musculus ATPase, H+ transporti...   324   3e-87
AF435091_1(AF435091|pid:none) Mus musculus vacuolar proton trans...   324   3e-87
AF472615_1(AF472615|pid:none) Danio rerio vacuolar-type H+ trans...   323   6e-87

 psg: 0.75  gvh: 0.36  alm: 0.30  top: 0.63  tms: 0.07  mit: 0.19  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vesicles of secretory system
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SLA124 is cyt

>SLA124F.Seq
GTTGGATTTGAAGATCATATCGCAGCTATCACAAGAAACTATCAAGTTAACCCACGTTTA
GATTATAGAACTGTTACAGCAGTAAATGGTCCACTCGTTATTTTAGATAATATCAGAGGT
CCAAAATTCTCTGAAATTGTAACTTTAACTTTAGGTGATGGTACTCAAAGAAAAGGTCAA
GTTTTGGAAATCCAAGGTAAAAGAGCTGTTGTACAAGTTTTCGAAGGTACAATTGGTATT
GATGCCAAACACACTCGTTGTGAATTCTCTGGTGATATTTTAAAGATGCCAGTATCAGAA
GATTCATTAGGTCGTATTTTCAATGGTTCAGGTAAACCAGTTGATAAAGGTCCAAATGTT
TTAGCTGAAGAATACTTAGATATTCAAGGTCAACCAATCAATCCATCAGTTAGAGTTTAC
CCACAAGAAATGATTCAAACTGGTATCTCTGCTATTGATACTATGAATTCAATTGCTCGT
GGTCAAAAGATTCCAATTTTCTCTGCTGCAGGTTTACCACACAATGAAATTGCCGCTCAA
ATTTGTCGTCAAGCTGGTTTAGTTAAAAAATCAGGTAAAGGTGTTATTGATGATCATGAA
GATAATTTTGCTATTGTATTCGCAGCTATGGGTGTTAATATGGAANNNNNNNNNN

>SLA124Z.Seq
NNNNNNNNNNCCTTGGNGGGGGNGCCCTTTNTTCTGGGGGTTACCCCNCCTTGGGNTTTT
TTGGCCGTTTCGGATNTNGCTTTNTCGGTCNAACCCCTTTTGGAAATCGGGGTTTCTTTT
ATTCCTTGGGTCTTGGNTGGNCTTTTTTTGGGNCTTTCCCCGGCNTTNNCCCCCANCGTT
TCCCTGGAAAANCTTTTTACCCATTCTTTTCCCGTTCCTCCAGGGGTTCTTTNGGTTCCC
CTTNCTTATTTTTTAAAGGGAAAAANTTTTAATCCAAAAAAAAAAANTTAAATTAAATTT
TAAAATTAAATTAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAA

>SLA124P.Seq
GTTGGATTTGAAGATCATATCGCAGCTATCACAAGAAACTATCAAGTTAACCCACGTTTA
GATTATAGAACTGTTACAGCAGTAAATGGTCCACTCGTTATTTTAGATAATATCAGAGGT
CCAAAATTCTCTGAAATTGTAACTTTAACTTTAGGTGATGGTACTCAAAGAAAAGGTCAA
GTTTTGGAAATCCAAGGTAAAAGAGCTGTTGTACAAGTTTTCGAAGGTACAATTGGTATT
GATGCCAAACACACTCGTTGTGAATTCTCTGGTGATATTTTAAAGATGCCAGTATCAGAA
GATTCATTAGGTCGTATTTTCAATGGTTCAGGTAAACCAGTTGATAAAGGTCCAAATGTT
TTAGCTGAAGAATACTTAGATATTCAAGGTCAACCAATCAATCCATCAGTTAGAGTTTAC
CCACAAGAAATGATTCAAACTGGTATCTCTGCTATTGATACTATGAATTCAATTGCTCGT
GGTCAAAAGATTCCAATTTTCTCTGCTGCAGGTTTACCACACAATGAAATTGCCGCTCAA
ATTTGTCGTCAAGCTGGTTTAGTTAAAAAATCAGGTAAAGGTGTTATTGATGATCATGAA
GATAATTTTGCTATTGTATTCGCAGCTATGGGTGTTAATATGGAA----------CCTTG
GNGGGGGNGCCCTTTNTTCTGGGGGTTACCCCNCCTTGGGNTTTTTTGGCCGTTTCGGAT
NTNGCTTTNTCGGTCNAACCCCTTTTGGAAATCGGGGTTTCTTTTATTCCTTGGGTCTTG
GNTGGNCTTTTTTTGGGNCTTTCCCCGGCNTTNNCCCCCANCGTTTCCCTGGAAAANCTT
TTTACCCATTCTTTTCCCGTTCCTCCAGGGGTTCTTTNGGTTCCCCTTNCTTATTTTTTA
AAGGGAAAAANTTTTAATCCAAAAAAAAAAANTTAAATTAAATTTTAAAATTAAATTAAA
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AA