>SHL684 (SHL684Q) /CSM/SH/SHL6-D/SHL684Q.Seq.d/
XXXXXXXXXXTCCATCATGGAATTTANCCGTGGTTCAACCAACCAGTGGTAATTATGTAC
CTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCA
CTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATC
GTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAA
TCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATC
GTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAAT
CTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCAC
CTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCA
TACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAA
CTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAA
TGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTG
ATGGTAAA

---PSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQS
SNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGK

Frame A:
---PSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHR
RTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQS
SNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQT
ITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGK


Frame B:
---hhgixpwfnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**civ
vl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnl
qtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihkl
sl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmv


Frame C:
---imefxrgstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddass
yfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstif
krlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstny
hfrsfnylfnnynhfcn*nefnwcskiikfikikmenc*w*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1298   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1298   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1298   0.0
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                       1298   0.0
VHO790 (VHO790Q) /CSM/VH/VHO7-D/VHO790Q.Seq.d/                       1298   0.0
VHO689 (VHO689Q) /CSM/VH/VHO6-D/VHO689Q.Seq.d/                       1298   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1298   0.0
VHO223 (VHO223Q) /CSM/VH/VHO2-A/VHO223Q.Seq.d/                       1298   0.0
VHM790 (VHM790Q) /CSM/VH/VHM7-D/VHM790Q.Seq.d/                       1298   0.0
VHK690 (VHK690Q) /CSM/VH/VHK6-D/VHK690Q.Seq.d/                       1298   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1275   0.0    1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.083  8
AP007341|AP007341.1 Lotus japonicus genomic DNA, chromosome 2, clone:LjT35G04, TM0425.    40   0.10   2
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   1.2    1
CT485998|CT485998.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-104K5.    32   2.8    6
AM025232|AM025232.1 Bos indicus 3' EST, clone C0005915l21.         34   3.0    2
BH703301|BH703301.1 BOMNG55TR BO_2_3_KB Brassica oleracea genomic clone BOMNG55, DNA sequence.    40   3.2    2
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   3.8    2
AL162752|AL162752.2 Neisseria meningitidis serogroup A strain Z2491 complete genome;  segment 1/7.    44   4.3    3
EH243429|EH243429.1 JGI_ACAC13551.fwd ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark) Phakopsora pachyrhizi cDNA clone ACAC13551 5', mRNA sequence.    44   4.6    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   370   e-101
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   370   e-101
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...    99   1e-19
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...    96   7e-19
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...    96   1e-18
EF146940_1(EF146940|pid:none) Populus trichocarpa clone WS01225_...    93   8e-18
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...    92   1e-17
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...    91   2e-17
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...    91   2e-17
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...    91   3e-17

 psg: 0.82  gvh: 0.50  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SHL684 is nuc

>SHL684Z.Seq
NNNNNNNNNNTCCATCATGGAATTTANCCGTGGTTCAACCAACCAGTGGTAATTATGTAC
CTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCA
CTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATC
GTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAA
TCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATC
GTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAAT
CTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCAC
CTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCA
TACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAA
CTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAA
TGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTG
ATGGTAAA