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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHL258
Library SH
(Link to library)
Clone ID SHL258
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SHL258P
(Link to Original site)
Representative DNA sequence 
>SHL258 (SHL258Q) /CSM/SH/SHL2-C/SHL258Q.Seq.d/
TTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATT
GGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGC
AGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGT
TTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCA
AACTCAACCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACC
ATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTT
TGATGGAAGAGATGTTTGGACATCAAATGTATCGGTTGGATTATATAATTTCAATGATAA
AACCAGAGCACTCGATGTAAATGGTAATGTTATTTCATCAATTACTACCGATGCTAATGG
TAGATATGCATTTGAAAATGTTGCCAATGGTGTTTATTGTTTATGGATGGAAGGTACAAA
ATATTTCAATCCAGGTTGCGTTGGTAGAATTGAAATCAACTCTGCAACAATGCCAXXXXX
XXXXXCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCATGACTATACCAA
TGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGGTTTCGATTACTA
TGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAAACAACTAAAAGG
TTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAACCTTTGGTAAACA
AAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACAATATCGTGTTGG
TAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGAACCTGGTATTTC
AGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAGTCAAACTGTATC
CGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTATTATTGTGTAAT
TCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGCTGATCCAACTCA
TCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTTATGTCACTTCTTT
TACTTCTTCTGATGCTGGTGGTTATTATTTTAATGGTTGTTCATTTGGTATTATTCCAAA
AAAATAAAGTAATAAAAAAATATAAATAAATAAAAAAATAAAAAAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
YEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGV
SSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPPPFTGSAVAGLLFTDINGNGIF
DGRDVWTSNVSVGLYNFNDKTRALDVNGNVISSITTDANGRYAFENVANGVYCLWMEGTK
YFNPGCVGRIEINSATMP---

---GNGNFFDNHKTGLHDYTNAFGATKGGRDIIVTTGFDYYEAFEGSIRWYNATSGKQLK
GYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSLPQYRVGRVWLDINGNGIQDAGEPGI
SGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSSNYYCVIPVAFFQAGSSITMRNADPT
HPNTNSDAFLSTVDNVYVTSFTSSDAGGYYFNGCSFGIIPKK*snkki*inkkikk*
Translated Amino Acid sequence (All Frames) 
Frame A:
l*iyiyqskrlll*nl*kdiglstrctmyssngfs**fgsrsfsckylcl*hsid*tswc
firliytys*n*c*fglc*ansttnsttysttysttntstiyw*crrwfiiyryqw*wnf
*wkrcldikcigwii*fq**nqstrckw*cyfinyyrc*w*ici*kccqwcllfmdgryk
ifqsrlrw*n*nqlcnna---

---qvmvisliiikqvfmtipmllvqlkvvvilllqlvsitmkhlkvqfvgimqplvnn*
kvtlyi*plqmvvphlqplvnkmvlvillqcmillfhnivlvefgli*mvmvfkmlvnlv
fqesqys*smvnnqlqqvklypiqmvisnlklisvqiiiv*fllhssklvlplq*emliq
liqiqivmlsyqllimfmsllllllmlvviilmvvhlvlfqknkvikkyk*ikk*kn

Frame B:
YEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGV
SSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPPPFTGSAVAGLLFTDINGNGIF
DGRDVWTSNVSVGLYNFNDKTRALDVNGNVISSITTDANGRYAFENVANGVYCLWMEGTK
YFNPGCVGRIEINSATMP---

---r*w*fl**s*nrss*lyqcfwcn*rws*yycynwfrll*si*rfnslv*cnlw*ttk
rllfiynlfkwsypisnlw*tkwsw*yyfsv*ffsstiscw*sla*ykw*wysrcw*twy
frnhsilnlwstinsnksncirfkwlfqi*s*fqfklllcnsccilpswffhynekc*sn
sskyk**cflinc**clchffyff*cwwllf*wlfiwyyskkik**knink*knkki

Frame C:
mnlhlpiqkiitlkpmkrhwlvhqvyhvfvqrfqliiwqqif*lqiplpitfh*lnklvf
hqahlylqlelvliwvmlsklnhqlnhllnhllnhqhlhhllvvpslvyylpismvmefl
meemfghqmyrldyiismikpehsm*mvmlfhqllpmlmvdmhlkmlpmvfivygwkvqn
isiqvalvelkstlqqc---

---GNGNFFDNHKTGLHDYTNAFGATKGGRDIIVTTGFDYYEAFEGSIRWYNATSGKQLK
GYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSLPQYRVGRVWLDINGNGIQDAGEPGI
SGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSSNYYCVIPVAFFQAGSSITMRNADPT
HPNTNSDAFLSTVDNVYVTSFTSSDAGGYYFNGCSFGIIPKK*snkki*inkkikk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL258 (SHL258Q) /CSM/SH/SHL2-C/SHL258Q.Seq.d/                       2432   0.0
SHJ874 (SHJ874Q) /CSM/SH/SHJ8-D/SHJ874Q.Seq.d/                       1292   0.0
SSG727 (SSG727Q) /CSM/SS/SSG7-B/SSG727Q.Seq.d/                       1292   0.0
SHL852 (SHL852Q) /CSM/SH/SHL8-C/SHL852Q.Seq.d/                       1292   0.0
SHL833 (SHL833Q) /CSM/SH/SHL8-B/SHL833Q.Seq.d/                       1292   0.0
SHL807 (SHL807Q) /CSM/SH/SHL8-A/SHL807Q.Seq.d/                       1292   0.0
SHL804 (SHL804Q) /CSM/SH/SHL8-A/SHL804Q.Seq.d/                       1292   0.0
SHL785 (SHL785Q) /CSM/SH/SHL7-D/SHL785Q.Seq.d/                       1292   0.0
SHL727 (SHL727Q) /CSM/SH/SHL7-B/SHL727Q.Seq.d/                       1292   0.0
SHL696 (SHL696Q) /CSM/SH/SHL6-D/SHL696Q.Seq.d/                       1292   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   4e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   4e-05  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   4e-05  1
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   4e-05  3
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   6e-04  3
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   6e-04  1
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   6e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   6e-04  1
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.002  3
DT775356|DT775356.1 124719991 TH1 Tribolium castaneum cDNA clone 3C7 3', mRNA sequence.    54   0.009  1
dna update 2006. 7. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    75   4e-12
(Q9KI14) RecName: Full=Serine-aspartate repeat-containing protei...    73   2e-11
(Q8NXX6) RecName: Full=Serine-aspartate repeat-containing protei...    69   5e-10
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    68   6e-10
(Q7A780) RecName: Full=Serine-aspartate repeat-containing protei...    67   1e-09
CP000703_575(CP000703|pid:none) Staphylococcus aureus subsp. aur...    67   1e-09
CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    67   1e-09
AM076203_1(AM076203|pid:none) Staphylococcus aureus partial sdrD...    67   1e-09
AM076200_1(AM076200|pid:none) Staphylococcus aureus partial sdrD...    67   1e-09
CP000686_1034(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    67   1e-09
protein update 2009. 5.30
PSORT 

 psg: 0.75  gvh: 0.54  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHL258 is cyt
5' end seq. ID SHL258F
5' end seq. 
>SHL258F.Seq
TTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATT
GGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGC
AGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGT
TTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCA
AACTCAACCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACC
ATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTT
TGATGGAAGAGATGTTTGGACATCAAATGTATCGGTTGGATTATATAATTTCAATGATAA
AACCAGAGCACTCGATGTAAATGGTAATGTTATTTCATCAATTACTACCGATGCTAATGG
TAGATATGCATTTGAAAATGTTGCCAATGGTGTTTATTGTTTATGGATGGAAGGTACAAA
ATATTTCAATCCAGGTTGCGTTGGTAGAATTGAAATCAACTCTGCAACAATGCCANNNNN
NNNNN
Length of 5' end seq. 605
3' end seq. ID SHL258Z
3' end seq. 
>SHL258Z.Seq
NNNNNNNNNNCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCATGACTAT
ACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGGTTTCGAT
TACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAAACAACTA
AAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAACCTTTGGT
AAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACAATATCGT
GTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGAACCTGGT
ATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAGTCAAACT
GTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTATTATTGT
GTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGCTGATCCA
ACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTTATGTCACT
TCTTTTACTTCTTCTGATGCTGGTGGTTATTATTTTAATGGTTGTTCATTTGGTATTATT
CCAAAAAAATAAAGTAATAAAAAAATATAAATAAATAAAAAAATAAAAAAATAA
Length of 3' end seq. 714
Connected seq. ID SHL258P
Connected seq. 
>SHL258P.Seq
TTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATT
GGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGC
AGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGT
TTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCA
AACTCAACCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACC
ATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTT
TGATGGAAGAGATGTTTGGACATCAAATGTATCGGTTGGATTATATAATTTCAATGATAA
AACCAGAGCACTCGATGTAAATGGTAATGTTATTTCATCAATTACTACCGATGCTAATGG
TAGATATGCATTTGAAAATGTTGCCAATGGTGTTTATTGTTTATGGATGGAAGGTACAAA
ATATTTCAATCCAGGTTGCGTTGGTAGAATTGAAATCAACTCTGCAACAATGCCA-----
-----CAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCATGACTATACCAA
TGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGGTTTCGATTACTA
TGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAAACAACTAAAAGG
TTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAACCTTTGGTAAACA
AAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACAATATCGTGTTGG
TAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGAACCTGGTATTTC
AGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAGTCAAACTGTATC
CGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTATTATTGTGTAAT
TCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGCTGATCCAACTCA
TCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTTATGTCACTTCTTT
TACTTCTTCTGATGCTGGTGGTTATTATTTTAATGGTTGTTCATTTGGTATTATTCCAAA
AAAATAAAGTAATAAAAAAATATAAATAAATAAAAAAATAAAAAAATAA
Length of connected seq. 1299
Full length Seq ID -
Full length Seq. -
Length of full length seq. -