>SHL173 (SHL173Q) /CSM/SH/SHL1-D/SHL173Q.Seq.d/
ATTATTAATATCAAGTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATGA
TCAACGTATTTTGGCATTAAAAGGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTA
TGATGAAGGTGAACCAGGATTTGGAAATGTTGAAATTTCTTTAGGAAATGCAACTGATTA
TAAGGGAAATAAATATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATGG
ATTAACTCAACGTACAACTTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTGA
AACCTATGAAAAGACATTGGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTC
AGCTGATAATTTGGCAGCAGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATT
GATTAAACAAGTTGGTGTTTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGA
TTTGGGTTATGTTGAGCAAACTCAACCACCAACTCAACCACCTACTCAACCACCTACTCA
ACCACCAACACCTCCACCATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATAT
CAATGGTAATGGAATTTTTGATGGAXXXXXXXXXX

LLISSIILNTVLNISSQVYDQRILALKGKCYLDSNKNALYDEGEPGFGNVEISLGNATDY
KGNKYPIRYSSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVS
ADNLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQ
PPTPPPFTGSAVAGLLFTDINGNGIFD---

Frame A:
iinikynfkycikyfitsi*styfgikrkmlfrfk*kciv**r*triwkc*nffrkcn*l
*gk*isntlfic*wyiyi*winstynl*iyiyqskrlll*nl*kdiglstrctmyssngf
s**fgsrsfsckylcl*hsid*tswcfirliytys*n*c*fglc*ansttnsttysttys
ttntstiyw*crrwfiiyryqw*wnf*w---


Frame B:
LLISSIILNTVLNISSQVYDQRILALKGKCYLDSNKNALYDEGEPGFGNVEISLGNATDY
KGNKYPIRYSSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVS
ADNLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQ
PPTPPPFTGSAVAGLLFTDINGNGIFD---


Frame C:
y*yqv*f*ily*ifhhkyminvfwh*kenvi*iqikmhcmmkvnqdlemlkfl*emqlii
reiniqyvihllmvhlymmd*lnvqlmnlhlpiqkiitlkpmkrhwlvhqvyhvfvqrfq
liiwqqif*lqiplpitfh*lnklvfhqahlylqlelvliwvmlsklnhqlnhllnhlln
hqhlhhllvvpslvyylpismvmeflm---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL696 (SHL696Q) /CSM/SH/SHL6-D/SHL696Q.Seq.d/                       1239   0.0
SHL536 (SHL536Q) /CSM/SH/SHL5-B/SHL536Q.Seq.d/                       1239   0.0
SHL313 (SHL313Q) /CSM/SH/SHL3-A/SHL313Q.Seq.d/                       1239   0.0
SHL215 (SHL215Q) /CSM/SH/SHL2-A/SHL215Q.Seq.d/                       1239   0.0
SHL173 (SHL173Q) /CSM/SH/SHL1-D/SHL173Q.Seq.d/                       1239   0.0
SHK534 (SHK534Q) /CSM/SH/SHK5-B/SHK534Q.Seq.d/                       1239   0.0
SHK516 (SHK516Q) /CSM/SH/SHK5-A/SHK516Q.Seq.d/                       1239   0.0
SHJ472 (SHJ472Q) /CSM/SH/SHJ4-C/SHJ472Q.Seq.d/                       1239   0.0
SHJ459 (SHJ459Q) /CSM/SH/SHJ4-C/SHJ459Q.Seq.d/                       1239   0.0
SHJ418 (SHJ418Q) /CSM/SH/SHJ4-A/SHJ418Q.Seq.d/                       1239   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   2e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   2e-05  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   2e-05  1
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   2e-05  3
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   3e-04  1
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   3e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   3e-04  1
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   3e-04  3
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.001  3
DT775356|DT775356.1 124719991 TH1 Tribolium castaneum cDNA clone 3C7 3', mRNA sequence.    54   0.004  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000284_2387(CP000284|pid:none) Methylobacillus flagellatus KT,...    49   9e-05
CP000686_1034(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    46   0.001
CP001337_286(CP001337|pid:none) Chloroflexus aggregans DSM 9485,...    42   0.018
BX294149_114(BX294149|pid:none) Rhodopirellula baltica SH 1 comp...    42   0.018
CP000830_3576(CP000830|pid:none) Dinoroseobacter shibae DFL 12, ...    40   0.041
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    38   0.27
CP000804_904(CP000804|pid:none) Roseiflexus castenholzii DSM 139...    37   0.59
CP000909_3116(CP000909|pid:none) Chloroflexus aurantiacus J-10-f...    37   0.59
CP000686_4190(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    36   0.78
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    36   0.78

 psg: 0.95  gvh: 0.46  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SHL173 is cyt

>SHL173F.Seq
ATTATTAATATCAAGTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATGA
TCAACGTATTTTGGCATTAAAAGGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTA
TGATGAAGGTGAACCAGGATTTGGAAATGTTGAAATTTCTTTAGGAAATGCAACTGATTA
TAAGGGAAATAAATATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATGG
ATTAACTCAACGTACAACTTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTGA
AACCTATGAAAAGACATTGGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTC
AGCTGATAATTTGGCAGCAGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATT
GATTAAACAAGTTGGTGTTTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGA
TTTGGGTTATGTTGAGCAAACTCAACCACCAACTCAACCACCTACTCAACCACCTACTCA
ACCACCAACACCTCCACCATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATAT
CAATGGTAATGGAATTTTTGATGGANNNNNNNNNN