>SHL165 (SHL165Q) /CSM/SH/SHL1-C/SHL165Q.Seq.d/
ATTTTACTAACATTGTTATTAAAATTTAAAATTTTTATATTAGATGATAAGTAATAGTAT
TAATGTAACTCCAAATAAAGAGTCAAAGATGTATGTTGTAAAAAGAGATGGTACAAAAGA
AAATGTATCATTTGATAAAATTACATCAAGAATTTCATTTTTATGTGAAATGGAACCAAA
ATTAAATTCAGATATAGTTGATCCAATTGAAGTAGCACAAAAAGTAGTTAGTGGAGTATT
TCCAGGTGTAAAGACTACAGAATTGGATAATTTGGCAGCCGAGACAGCAGCATATATGTC
AACACGTCATCCAGATTATGGTATTTTGGCAGCACGTATTAGTGTATCGAATCTCCATAA
ACAAACTTCAAAGAGTTTTGTAGAGACAGTTAAGAAACAATATGAATTCATTAATCCAAA
GAGCAATTTATTGGCACCATTGGTCAGCAAGGAGCTCTATGAAATCGTAATGAAACATGG
TGAGAGATTGGAGAGTGTCATTGATTATTATCGTGATTTCGATTACGATTTCTTTGGATT
CAAAACATTGGAACGTTCCTATCTTCATCGTATCAATGGTAAAATTGTCGAGCGTCCACA
ACACATGTTGATGCGTGTCTCCATTGGTATCXXXXXXXXXXTTTACGTAATTCACTCCTT
CATTGCACCAATGCCAACCGCTTCCAACAAGTCAAATCCTTGGTAACAATGAATGCTTTG
AACCTTACACTTCCAACATTTACAGTCGTCGTGTTTTAGCAGGTGAATTCACAATTGTCA
ACAAACAATTACTCGAAGATCTCATGGAATTGGGCATTTGGTCACCAGAGATGAAGAATC
AAATCGTTGCTGCACGTGGTTCCATTCAATCCATCGGTGGCATCCCTGATGATTTGAAAG
AACTCTATAAAACAGTTTGGGAAATCCGTCAAAGAACTCTCATCGATATGGCTGCTGATC
GTGGTGCTTTCATCGATCAAAGTCAAAGTTTCAATGTTTTCATTGCTGAACCAACCTTTG
CTAAACTCACCTCAATGCATTTCTACTCATGGAAGAAAGGTTTAAAGACTGGTATGTACT
ATCTTCGTACTCGTCCTGCCGCTGATGCAATTCAATTCACTGTTGATCCAATCGTATCTC
AAACTCCAAAACCAGTTGAAAATAATATAAACTCAACTACACCACTTAAAACTCCAACTA
AAACTCCAAACTCTTCAAATCGTATCTCTACTAGTCCAACTAATAATTTAACAAGTCCAA
TTCGTTTTAATATAACCCAACAACAACAACAACAGCAACAACAACAACAACAACAAAATA
ATAAGAAGAGATTGGCTAATTA

fy*hcy*nlkfly*MISNSINVTPNKESKMYVVKRDGTKENVSFDKITSRISFLCEMEPK
LNSDIVDPIEVAQKVVSGVFPGVKTTELDNLAAETAAYMSTRHPDYGILAARISVSNLHK
QTSKSFVETVKKQYEFINPKSNLLAPLVSKELYEIVMKHGERLESVIDYYRDFDYDFFGF
KTLERSYLHRINGKIVERPQHMLMRVSIGI---

---LRNSLLHCTNANRFQQVKSLVTMNALNLTLPTFTVVVF*qvnsqlstnnyskiswnw
afghqr*riksllhvvpfnpsvaslmi*knsikqfgksvkelssiwllivvlssikvkvs
mfsllnqpllnspqcisthgrkv*rlvctifvlvlplmqfnslliqsylklqnqlkii*t
qlhhlklqlklqtlqivsllvqlii*qvqfvli*pnnnnnsnnnnnnkiirrdwli

Frame A:
illtlllkfkifilddk**y*cnsk*rvkdvcckkrwykrkcii**nyiknfifm*ngtk
ikfrys*sn*sstkss*wsisrckdyrig*fgsrdssiyvntssrlwyfgsty*ciesp*
tnfkefcrds*eti*ih*skeqfigtigqqgal*nrnetw*eigech*lls*frlrflwi
qnigtflsssyqw*ncrastthvdaclhwy---

---ft*ftpslhqcqplptsqilgnnecfepytsniysrrvlageftivnkqlledlmel
giwspemknqivaargsiqsiggipddlkelyktvweirqrtlidmaadrgafidqsqsf
nvfiaeptfakltsmhfyswkkglktgmyylrtrpaadaiqftvdpivsqtpkpvennin
sttplktptktpnssnristsptnnltspirfnitqqqqqqqqqqqqqnnkkrlan

Frame B:
fy*hcy*nlkfly*MISNSINVTPNKESKMYVVKRDGTKENVSFDKITSRISFLCEMEPK
LNSDIVDPIEVAQKVVSGVFPGVKTTELDNLAAETAAYMSTRHPDYGILAARISVSNLHK
QTSKSFVETVKKQYEFINPKSNLLAPLVSKELYEIVMKHGERLESVIDYYRDFDYDFFGF
KTLERSYLHRINGKIVERPQHMLMRVSIGI---

---LRNSLLHCTNANRFQQVKSLVTMNALNLTLPTFTVVVF*qvnsqlstnnyskiswnw
afghqr*riksllhvvpfnpsvaslmi*knsikqfgksvkelssiwllivvlssikvkvs
mfsllnqpllnspqcisthgrkv*rlvctifvlvlplmqfnslliqsylklqnqlkii*t
qlhhlklqlklqtlqivsllvqlii*qvqfvli*pnnnnnsnnnnnnkiirrdwli

Frame C:
ftniviki*nfyir**vivlm*lqiksqrcml*kemvqkkmyhliklhqefhfyvkwnqn
*iqi*liqlk*hkk*lveyfqv*rlqnwiiwqprqqhicqhviqimvfwqhvlvyrisin
klqrvl*rqlrnnmnsliqraiywhhwsarssmks**nmvrdwrvsliiivisitislds
khwnvpifivsmvklssvhntc*cvsplv---

---yvihsfiapmptasnksnpw*q*ml*tlhfqhlqsscfsr*ihncqqtitrrshgig
hlvtrdeesnrcctwfhsihrwhp**fertl*nslgnpsknshrygc*swcfhrskskfq
cfhc*tnlc*thlnafllmeerfkdwyvlssysscr*cnsihc*snrisnskts*k*ykl
nytt*nsn*nsklfksyly*sn**fnksnsf*ynptttttattttttk**eeig*l

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL165 (SHL165Q) /CSM/SH/SHL1-C/SHL165Q.Seq.d/                       2472   0.0
VHC688 (VHC688Q) /CSM/VH/VHC6-D/VHC688Q.Seq.d/                       1243   0.0
SHA878 (SHA878Q) /CSM/SH/SHA8-D/SHA878Q.Seq.d/                       1243   0.0
AHR370 (AHR370Q) /CSM/AH/AHR3-C/AHR370Q.Seq.d/                       1243   0.0
AHG718 (AHG718Q) /CSM/AH/AHG7-A/AHG718Q.Seq.d/                       1243   0.0
AHB722 (AHB722Q) /CSM/AH/AHB7-A/AHB722Q.Seq.d/                       1243   0.0
SHK512 (SHK512Q) /CSM/SH/SHK5-A/SHK512Q.Seq.d/                       1235   0.0
CHJ284 (CHJ284Q) /CSM/CH/CHJ2-D/CHJ284Q.Seq.d/                       1235   0.0
CHG573 (CHG573Q) /CSM/CH/CHG5-D/CHG573Q.Seq.d/                       1235   0.0
SHE353 (SHE353Q) /CSM/SH/SHE3-C/SHE353Q.Seq.d/                       1233   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

DY893053|DY893053.1 CeleSEQ12221 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ12221, mRNA sequence.    96   6e-35  2
AL440930|AL440930.1 T7 end of clone BD0AA015G01 of library BD0AA from strain CBS 94 of  Candida tropicalis.    70   2e-21  4
AJ390500|AJ390500.1 Candida albicans partial mRNA for ribonucleotide reductase large subunit (rnr1 gene).    64   3e-19  4
AX489294|AX489294.1 Sequence 6594 from Patent WO02053728.          64   3e-19  4
AR548770|AR548770.1 Sequence 3901 from patent US 6747137.          64   4e-19  4
Z19158|Z19158.1 Caenorhabditis elegans Cosmid T23G5.               48   4e-16  8
AW334328|AW334328.1 S33D5 AGS-1 Pneumocystis carinii f. sp. carinii cDNA 3', mRNA sequence.    52   7e-16  4
AW333566|AW333566.1 S23D1 AGS-1 Pneumocystis carinii f. sp. carinii cDNA 3', mRNA sequence.    48   1e-13  4
AL403114|AL403114.1 T3 end of clone AT0AA006F08 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    54   2e-11  6
DQ332881|DQ332881.1 Synthetic construct Saccharomyces cerevisiae clone FLH115353.01X RNR3 gene, complete sequence.    54   3e-11  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54Q71) RecName: Full=Ribonucleoside-diphosphate reductase larg...   359   1e-97
CR954201_10(CR954201|pid:none) Ostreococcus tauri strain OTTH059...   236   1e-60
CP001574_753(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   236   2e-60
AF092841_1(AF092841|pid:none) Arabidopsis thaliana ribonucleosid...   234   7e-60
AP004240_6(AP004240|pid:none) Oryza sativa Japonica Group genomi...   231   3e-59
CP000581_13(CP000581|pid:none) Ostreococcus lucimarinus CCE9901 ...   231   3e-59
AF118785_1(AF118785|pid:none) Glycine max ribonucleotide reducta...   231   6e-59
(P07742) RecName: Full=Ribonucleoside-diphosphate reductase larg...   230   1e-58
BC109486_1(BC109486|pid:none) Bos taurus ribonucleotide reductas...   230   1e-58
AM437010_1(AM437010|pid:none) Vitis vinifera contig VV78X108426....   230   1e-58

 psg: 0.79  gvh: 0.38  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SHL165 is cyt

>SHL165F.Seq
ATTTTACTAACATTGTTATTAAAATTTAAAATTTTTATATTAGATGATAAGTAATAGTAT
TAATGTAACTCCAAATAAAGAGTCAAAGATGTATGTTGTAAAAAGAGATGGTACAAAAGA
AAATGTATCATTTGATAAAATTACATCAAGAATTTCATTTTTATGTGAAATGGAACCAAA
ATTAAATTCAGATATAGTTGATCCAATTGAAGTAGCACAAAAAGTAGTTAGTGGAGTATT
TCCAGGTGTAAAGACTACAGAATTGGATAATTTGGCAGCCGAGACAGCAGCATATATGTC
AACACGTCATCCAGATTATGGTATTTTGGCAGCACGTATTAGTGTATCGAATCTCCATAA
ACAAACTTCAAAGAGTTTTGTAGAGACAGTTAAGAAACAATATGAATTCATTAATCCAAA
GAGCAATTTATTGGCACCATTGGTCAGCAAGGAGCTCTATGAAATCGTAATGAAACATGG
TGAGAGATTGGAGAGTGTCATTGATTATTATCGTGATTTCGATTACGATTTCTTTGGATT
CAAAACATTGGAACGTTCCTATCTTCATCGTATCAATGGTAAAATTGTCGAGCGTCCACA
ACACATGTTGATGCGTGTCTCCATTGGTATCNNNNNNNNNN

>SHL165Z.Seq
NNNNNNNNNNTTTACGTAATTCACTCCTTCATTGCACCAATGCCAACCGCTTCCAACAAG
TCAAATCCTTGGTAACAATGAATGCTTTGAACCTTACACTTCCAACATTTACAGTCGTCG
TGTTTTAGCAGGTGAATTCACAATTGTCAACAAACAATTACTCGAAGATCTCATGGAATT
GGGCATTTGGTCACCAGAGATGAAGAATCAAATCGTTGCTGCACGTGGTTCCATTCAATC
CATCGGTGGCATCCCTGATGATTTGAAAGAACTCTATAAAACAGTTTGGGAAATCCGTCA
AAGAACTCTCATCGATATGGCTGCTGATCGTGGTGCTTTCATCGATCAAAGTCAAAGTTT
CAATGTTTTCATTGCTGAACCAACCTTTGCTAAACTCACCTCAATGCATTTCTACTCATG
GAAGAAAGGTTTAAAGACTGGTATGTACTATCTTCGTACTCGTCCTGCCGCTGATGCAAT
TCAATTCACTGTTGATCCAATCGTATCTCAAACTCCAAAACCAGTTGAAAATAATATAAA
CTCAACTACACCACTTAAAACTCCAACTAAAACTCCAAACTCTTCAAATCGTATCTCTAC
TAGTCCAACTAATAATTTAACAAGTCCAATTCGTTTTAATATAACCCAACAACAACAACA
ACAGCAACAACAACAACAACAACAAAATAATAAGAAGAGATTGGCTAATTA

>SHL165P.Seq
ATTTTACTAACATTGTTATTAAAATTTAAAATTTTTATATTAGATGATAAGTAATAGTAT
TAATGTAACTCCAAATAAAGAGTCAAAGATGTATGTTGTAAAAAGAGATGGTACAAAAGA
AAATGTATCATTTGATAAAATTACATCAAGAATTTCATTTTTATGTGAAATGGAACCAAA
ATTAAATTCAGATATAGTTGATCCAATTGAAGTAGCACAAAAAGTAGTTAGTGGAGTATT
TCCAGGTGTAAAGACTACAGAATTGGATAATTTGGCAGCCGAGACAGCAGCATATATGTC
AACACGTCATCCAGATTATGGTATTTTGGCAGCACGTATTAGTGTATCGAATCTCCATAA
ACAAACTTCAAAGAGTTTTGTAGAGACAGTTAAGAAACAATATGAATTCATTAATCCAAA
GAGCAATTTATTGGCACCATTGGTCAGCAAGGAGCTCTATGAAATCGTAATGAAACATGG
TGAGAGATTGGAGAGTGTCATTGATTATTATCGTGATTTCGATTACGATTTCTTTGGATT
CAAAACATTGGAACGTTCCTATCTTCATCGTATCAATGGTAAAATTGTCGAGCGTCCACA
ACACATGTTGATGCGTGTCTCCATTGGTATC----------TTTACGTAATTCACTCCTT
CATTGCACCAATGCCAACCGCTTCCAACAAGTCAAATCCTTGGTAACAATGAATGCTTTG
AACCTTACACTTCCAACATTTACAGTCGTCGTGTTTTAGCAGGTGAATTCACAATTGTCA
ACAAACAATTACTCGAAGATCTCATGGAATTGGGCATTTGGTCACCAGAGATGAAGAATC
AAATCGTTGCTGCACGTGGTTCCATTCAATCCATCGGTGGCATCCCTGATGATTTGAAAG
AACTCTATAAAACAGTTTGGGAAATCCGTCAAAGAACTCTCATCGATATGGCTGCTGATC
GTGGTGCTTTCATCGATCAAAGTCAAAGTTTCAATGTTTTCATTGCTGAACCAACCTTTG
CTAAACTCACCTCAATGCATTTCTACTCATGGAAGAAAGGTTTAAAGACTGGTATGTACT
ATCTTCGTACTCGTCCTGCCGCTGATGCAATTCAATTCACTGTTGATCCAATCGTATCTC
AAACTCCAAAACCAGTTGAAAATAATATAAACTCAACTACACCACTTAAAACTCCAACTA
AAACTCCAAACTCTTCAAATCGTATCTCTACTAGTCCAACTAATAATTTAACAAGTCCAA
TTCGTTTTAATATAACCCAACAACAACAACAACAGCAACAACAACAACAACAACAAAATA
ATAAGAAGAGATTGGCTAATTA