>SHL153 (SHL153Q) /CSM/SH/SHL1-C/SHL153Q.Seq.d/
GAAAAATTTAAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAA
ATGGCTACACCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCAT
GTATTAGATAATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGA
CAAGATCATGAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGA
CAATATTGTATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGAT
AAATTCAATCAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCA
TTCCCATTATATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAA
ACCAATAGTGCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAA
ACCACAGTCAGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTAT
CCACGTGTTGAAGTTCXXXXXXXXXXCGATATCATTCAACAAGCCGTTTTCGGTTCAACC
GATGGTTCAAGCAATGATTGCTTATACTTTGAAACCAATGGTTTGGTCATCACAAACATT
GATGTTCAATCTGTAGAACCAGTCGATCAACGTACTCTTGATTCTTTACAAAAATCTGTT
CAATTAGCCATTGAAATTACCACCAAATCACAAGAAGCCACTGCTCGTCAAGAAGCTGAA
CGTTTAGAACAAATGGCTCGTGGTGAACTTGAACGTCAAAAGATTATCGATGAAGCCAAG
AACGAAGAATCTCGTTCCAAATTGGTCCAATTACAAGCTCAATCAGCTGCTGTCGAATCA
ACTGGTCAAGCTGTTGCTGAAGCTCGTGCTCGTGCTGAAGCCGCCATCATTGAAGCTGAA
AGTGAAATCAAACAAGCTCGTCTCTCAACCAGAGCCATTGAAATTGAAGCTCTCTCTGAA
ATTGAAAATCTTAAAGCCAAACATATTGTTGAAATCCAACACATCAAATCACTCAACAAT
CTCGAATTAATTAAAGCCAAAGAAAGTGCTACCATCGAAACTACCAAATTCGAAAACTAT
GTTGAAGCCCTCGGTTCCGATACTATCAAATCAATCGCCCAAGCTCCAGAAGAAACCAAG
GCTAAATTATTAGCTGGTTTGGGTCTCAAATCATTCATGATTACTGATGGTAAATCACCA
CTCAATCTTTTCGATACTGCCAAGGTTTAATTGCTGATGCTCAAAGATCAGAAGTCGNAN
AAGNAATAAAATTTTTA

ekfklfspnlfliyk*YIYKMATPVQASTVIRVKPHHYIHVLDNNTNVTRVEVGPQTFTR
QDHERVLNPTPLPMILIPPRQYCIIENPIIRDKEGKLVVDKFNQPKLRHGDQEIRFSQDP
FPLYPGEVVIAQPVPLQVIQTNSALRLKCLRDFVEVKADKTTVSHIAGDEWLFEGPGTYY
PRVEV---

---DIIQQAVFGSTDGSSNDCLYFETNGLVITNIDVQSVEPVDQRTLDSLQKSVQLAIEI
TTKSQEATARQEAERLEQMARGELERQKIIDEAKNEESRSKLVQLQAQSAAVESTGQAVA
EARARAEAAIIEAESEIKQARLSTRAIEIEALSEIENLKAKHIVEIQHIKSLNNLELIKA
KESATIETTKFENYVEALGSDTIKSIAQAPEETKAKLLAGLGLKSFMITDGKSPLNLFDT
AKV*llmlkdqksxkx*nf

Frame A:
ekfklfspnlfliyk*YIYKMATPVQASTVIRVKPHHYIHVLDNNTNVTRVEVGPQTFTR
QDHERVLNPTPLPMILIPPRQYCIIENPIIRDKEGKLVVDKFNQPKLRHGDQEIRFSQDP
FPLYPGEVVIAQPVPLQVIQTNSALRLKCLRDFVEVKADKTTVSHIAGDEWLFEGPGTYY
PRVEV---

---ryhstsrfrfnrwfkq*llil*nqwfghhkh*csicrtsrstys*fftkicsish*n
yhqitrshcssrs*tfrtngsw*t*tskdyr*sqerrisfqigpitssisccrinwsscc
*sscsc*srhh*s*k*nqtssslnqsh*n*ssl*n*ks*sqtyc*npthqitqqsrin*s
qrkcyhrnyqirklc*sprfryyqinrpssrrnqg*iiswfgsqiihdy*w*ittqsfry
cqgliadaqrsevxxxikfl

Frame B:
knlnyflqiyf*ytnniyikwlhqsklll*fvlnhiiifmy*iiiqmlqelkldhkhlld
kimnvy*iqlhyq*f*shqdnivllktqlleikkvn*llinsinqnsvmvikkfvslkih
shyiqvklsllnqfhyksskpivhfv*nvfvislkskltkpqsvtllvmngyskvqvpii
hvlkf---

---DIIQQAVFGSTDGSSNDCLYFETNGLVITNIDVQSVEPVDQRTLDSLQKSVQLAIEI
TTKSQEATARQEAERLEQMARGELERQKIIDEAKNEESRSKLVQLQAQSAAVESTGQAVA
EARARAEAAIIEAESEIKQARLSTRAIEIEALSEIENLKAKHIVEIQHIKSLNNLELIKA
KESATIETTKFENYVEALGSDTIKSIAQAPEETKAKLLAGLGLKSFMITDGKSPLNLFDT
AKV*llmlkdqksxkx*nf

Frame C:
ki*iifskfifniqiiyi*ngytspsfycnsc*ttslyscir**ykcyks*swttniy*t
rs*tciksnsitndfdptktilyy*kpny*r*rr*isc**iqstktpsw*srnsflsrsi
piisr*schcstssitshpnq*ctsfemss*fr*sqs*qnhsqshcw**mvirrsrylls
tc*s---

---isfnkpfsvqpmvqamiaytlkpmvwssqtlmfnl*nqsinvllilyknlfn*plkl
ppnhkkpllvkklnv*nkwlvvnlnvkrlsmkprtknlvpnwsnyklnqllsnqlvklll
klvlvlkppslklkvksnklvsqpeplklklslklkilkpnillksntsnhstisn*lkp
kkvlpsklpnsktmlkpsvpilsnqspklqkkprlny*lvwvsnhs*llmvnhhsifsil
prfnc*cskirsrxxnkif

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL153 (SHL153Q) /CSM/SH/SHL1-C/SHL153Q.Seq.d/                       2450   0.0
AFK544 (AFK544Q) /CSM/AF/AFK5-B/AFK544Q.Seq.d/                       1481   0.0
AHL591 (AHL591Q) /CSM/AH/AHL5-D/AHL591Q.Seq.d/                       1475   0.0
VHJ686 (VHJ686Q) /CSM/VH/VHJ6-D/VHJ686Q.Seq.d/                       1429   0.0
SLB219 (SLB219Q) /CSM/SL/SLB2-A/SLB219Q.Seq.d/                       1421   0.0
AHL354 (AHL354Q) /CSM/AH/AHL3-C/AHL354Q.Seq.d/                       1407   0.0
VHI718 (VHI718Q) /CSM/VH/VHI7-A/VHI718Q.Seq.d/                       1400   0.0
VHI266 (VHI266Q) /CSM/VH/VHI2-C/VHI266Q.Seq.d/                       1400   0.0
AHC287 (AHC287Q) /CSM/AH/AHC2-D/AHC287Q.Seq.d/                       1394   0.0
VHM426 (VHM426Q) /CSM/VH/VHM4-B/VHM426Q.Seq.d/                       1380   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Z37109|Z37109.1 D.discoideum mRNA for major vault protein B.     1421   0.0    2
L08646|L08646.1 Dictyostelium discoideum major vault protein alpha (MVPalpha) mRNA, complete cds.   196   e-133  6
EC132986|EC132986.1 HVE00006496 Hartmannella vermiformis Regular Large Hartmannella vermiformis cDNA, mRNA sequence.    76   2e-14  2
CX835130|AF255664_1 major vault protein [Ictalurus punctatus], mRNA sequence.    52   6e-08  2
CK740021|CK740021.1 USDA-FP_6515 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE64E02 5', mRNA sequence.    68   6e-07  1
DN137794| MVP100 [Torpedo marmorata], mRNA sequence.    44   1e-05  2
CV681753|CV681753.1 sjs3-011_F10_sjs3-011F10-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    48   3e-05  3
CV464582|CV464582.1 taj22f05.y1 Hydra EST UCI 5 ALP Hydra magnipapillata cDNA 5' similar to SW:MVP_HUMAN Q14764 MAJOR VAULT PROTEIN ;, mRNA sequence.    54   5e-05  2
DN137231| unnamed protein product [Mus musculus], mRNA sequence.    54   0.009  1
CV566925|CV566925.1 tak02h03.y1 Hydra EST UCI 6 Hydra magnipapillata cDNA 5' similar to SW:MVP_HUMAN Q14764 MAJOR VAULT PROTEIN ;, mRNA sequence.    54   0.009  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54659) RecName: Full=Major vault protein beta;          Short=...   387   e-107
AC116668_48(AC116668|pid:none) Trypanosoma brucei chromosome 5 c...   197   5e-49
(Q62667) RecName: Full=Major vault protein;          Short=MVP; ...   192   2e-47
AK167899_1(AK167899|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   192   2e-47
AK088046_1(AK088046|pid:none) Mus musculus 2 days neonate thymus...   192   2e-47
(Q9EQK5) RecName: Full=Major vault protein;          Short=MVP; ...   192   2e-47
(Q90405) RecName: Full=Major vault protein; AltName: Full=MVP100...   192   3e-47
(Q6PF69) RecName: Full=Major vault protein;          Short=MVP; ...   191   4e-47
BC049344_1(BC049344|pid:none) Danio rerio major vault protein, m...   190   1e-46
AK152534_1(AK152534|pid:none) Mus musculus bone marrow macrophag...   189   2e-46

 psg: 0.83  gvh: 0.28  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for SHL153 is cyt

>SHL153F.Seq
GAAAAATTTAAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAA
ATGGCTACACCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCAT
GTATTAGATAATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGA
CAAGATCATGAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGA
CAATATTGTATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGAT
AAATTCAATCAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCA
TTCCCATTATATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAA
ACCAATAGTGCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAA
ACCACAGTCAGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTAT
CCACGTGTTGAAGTTCNNNNNNNNNN

>SHL153Z.Seq
NNNNNNNNNNCGATATCATTCAACAAGCCGTTTTCGGTTCAACCGATGGTTCAAGCAATG
ATTGCTTATACTTTGAAACCAATGGTTTGGTCATCACAAACATTGATGTTCAATCTGTAG
AACCAGTCGATCAACGTACTCTTGATTCTTTACAAAAATCTGTTCAATTAGCCATTGAAA
TTACCACCAAATCACAAGAAGCCACTGCTCGTCAAGAAGCTGAACGTTTAGAACAAATGG
CTCGTGGTGAACTTGAACGTCAAAAGATTATCGATGAAGCCAAGAACGAAGAATCTCGTT
CCAAATTGGTCCAATTACAAGCTCAATCAGCTGCTGTCGAATCAACTGGTCAAGCTGTTG
CTGAAGCTCGTGCTCGTGCTGAAGCCGCCATCATTGAAGCTGAAAGTGAAATCAAACAAG
CTCGTCTCTCAACCAGAGCCATTGAAATTGAAGCTCTCTCTGAAATTGAAAATCTTAAAG
CCAAACATATTGTTGAAATCCAACACATCAAATCACTCAACAATCTCGAATTAATTAAAG
CCAAAGAAAGTGCTACCATCGAAACTACCAAATTCGAAAACTATGTTGAAGCCCTCGGTT
CCGATACTATCAAATCAATCGCCCAAGCTCCAGAAGAAACCAAGGCTAAATTATTAGCTG
GTTTGGGTCTCAAATCATTCATGATTACTGATGGTAAATCACCACTCAATCTTTTCGATA
CTGCCAAGGTTTAATTGCTGATGCTCAAAGATCAGAAGTCGNANAAGNAATAAAATTTTT
A

>SHL153P.Seq
GAAAAATTTAAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAA
ATGGCTACACCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCAT
GTATTAGATAATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGA
CAAGATCATGAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGA
CAATATTGTATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGAT
AAATTCAATCAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCA
TTCCCATTATATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAA
ACCAATAGTGCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAA
ACCACAGTCAGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTAT
CCACGTGTTGAAGTTC----------CGATATCATTCAACAAGCCGTTTTCGGTTCAACC
GATGGTTCAAGCAATGATTGCTTATACTTTGAAACCAATGGTTTGGTCATCACAAACATT
GATGTTCAATCTGTAGAACCAGTCGATCAACGTACTCTTGATTCTTTACAAAAATCTGTT
CAATTAGCCATTGAAATTACCACCAAATCACAAGAAGCCACTGCTCGTCAAGAAGCTGAA
CGTTTAGAACAAATGGCTCGTGGTGAACTTGAACGTCAAAAGATTATCGATGAAGCCAAG
AACGAAGAATCTCGTTCCAAATTGGTCCAATTACAAGCTCAATCAGCTGCTGTCGAATCA
ACTGGTCAAGCTGTTGCTGAAGCTCGTGCTCGTGCTGAAGCCGCCATCATTGAAGCTGAA
AGTGAAATCAAACAAGCTCGTCTCTCAACCAGAGCCATTGAAATTGAAGCTCTCTCTGAA
ATTGAAAATCTTAAAGCCAAACATATTGTTGAAATCCAACACATCAAATCACTCAACAAT
CTCGAATTAATTAAAGCCAAAGAAAGTGCTACCATCGAAACTACCAAATTCGAAAACTAT
GTTGAAGCCCTCGGTTCCGATACTATCAAATCAATCGCCCAAGCTCCAGAAGAAACCAAG
GCTAAATTATTAGCTGGTTTGGGTCTCAAATCATTCATGATTACTGATGGTAAATCACCA
CTCAATCTTTTCGATACTGCCAAGGTTTAATTGCTGATGCTCAAAGATCAGAAGTCGNAN
AAGNAATAAAATTTTTA