>AFJ752 (AFJ752Q) /CSM/AF/AFJ7-C/AFJ752Q.Seq.d/
XXXXXXXXXXAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTG
ATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATA
CCGTCTCCAAATCGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTC
AACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAA
AGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTC
ATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAAT
CTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTA
GAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATG
AAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATNA
ATTCAAATTACCATCATCTCAAACTGANATAATGTNAATCTAAAAAAGATTCTTCTGCTA
A

---ATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKSIQDVIGGKVSPFVKSQ
PEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVES
VSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAXN
SNYHHLKLX*cxskkdssa

Frame A:
---ATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKSIQDVIGGKVSPFVKSQ
PEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVES
VSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAXN
SNYHHLKLX*cxskkdssa


Frame B:
---qliwvslvklfqpslliqlpiklvtismkkkpshsipspnrskmslvvkflhllnln
qnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnl
sqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlpxi
qitiisn*xnvnlkkilll


Frame C:
---nsygslw*scsshlc*fscq*sslqfr*krnlliryrlqidprchww*sfsic*ist
rtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*ic
lnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rchxf
klpssqtximxi*krffc*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ752 (AFJ752Q) /CSM/AF/AFJ7-C/AFJ752Q.Seq.d/                       1154   0.0
AFL143 (AFL143Q) /CSM/AF/AFL1-B/AFL143Q.Seq.d/                       1146   0.0
AFL139 (AFL139Q) /CSM/AF/AFL1-B/AFL139Q.Seq.d/                       1146   0.0
AFJ830 (AFJ830Q) /CSM/AF/AFJ8-B/AFJ830Q.Seq.d/                       1146   0.0
AFJ339 (AFJ339Q) /CSM/AF/AFJ3-B/AFJ339Q.Seq.d/                       1140   0.0
AFL121 (AFL121Q) /CSM/AF/AFL1-A/AFL121Q.Seq.d/                       1134   0.0
AFK858 (AFK858Q) /CSM/AF/AFK8-C/AFK858Q.Seq.d/                       1134   0.0
AFK136 (AFK136Q) /CSM/AF/AFK1-B/AFK136Q.Seq.d/                       1134   0.0
AFH744 (AFH744Q) /CSM/AF/AFH7-B/AFH744Q.Seq.d/                       1134   0.0
AFO713 (AFO713Q) /CSM/AF/AFO7-A/AFO713Q.Seq.d/                       1132   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  2e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  3e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  3e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  1e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  2e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  9e-06  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  3e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  3e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  3e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   164   2e-39
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   158   1e-37
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   157   2e-37
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   155   6e-37
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   153   4e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   150   3e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   147   2e-34
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   147   2e-34
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   147   2e-34
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   147   3e-34

 psg: 0.79  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFJ752 is nuc

>AFJ752Z.Seq
----------AGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTG
ATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATA
CCGTCTCCAAATCGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTC
AACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAA
AGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTC
ATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAAT
CTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTA
GAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATG
AAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATNA
ATTCAAATTACCATCATCTCAAACTGANATAATGTNAATCTAAAAAAGATTCTTCTGCTA
A