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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK887
Library SH
(Link to library)
Clone ID SHK887
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12649-1|Contig-U16394-1
Original site URL
Representative seq. ID SHK887P
(Link to Original site)
Representative DNA sequence 
>SHK887 (SHK887Q) /CSM/SH/SHK8-D/SHK887Q.Seq.d/
AACACCAATAAATAATAATATTAATAATAATAATAATAATAATAATAATAATAATAAAAT
AAAAAATATAATAATAATAATAGTAATAATAGTAATAATAATAATAAAATAATAATAATA
GAAATAATTTCAATTTAAAAAATAAATAAAATAAAAAAAGGAAAAAATAAAAAAAAAAAT
AAATTTTTTTTTTAAAAAATTAAAANTTTAAAAAAAAAAXXXXXXXXXXTCTTTCAATTC
ACCATGCAAATGACATTGGGTGTACAGTGTCTTCACAATTGGACACCTCAAATTGTACAC
AGAGACTTTAAGAGTTTAAATCTTTTGGTCAATGAAGATTGGGAATGCAAAGTTTCAGAT
TTTGGTCTTAGTCGTTTTAATACCGCCGATAATTTAGAAACACTCAGTAAGATTCGTGGT
ACCTTTGCCTATTGTTCACCTGAAGTCGCCGTAGGTAATGGTAGTCTCTACACTACCAAA
TCTGATATCTACAGTATTGGTATCGTCTTTTGGGAATTAGTAACTCGTGTTATCAATGGT
GAATACTCTCGTCCCTACAGTGAATATTCTCACATCAAAATGGACTTTCAAATCATGTTA
AACTCAAAGGAAGGTTTAAGACCAACCTTACCTCAAAATACTCCACTTG
sequence update 2002.10.25
Translated Amino Acid sequence 
ntnk**y************nkkynnnnsnnsnnnnkiiiieiisi*kinkikkgknkkkn
kfff*KIKXLKKK---

---FQFTMQMTLGVQCLHNWTPQIVHRDFKSLNLLVNEDWECKVSDFGLSRFNTADNLET
LSKIRGTFAYCSPEVAVGNGSLYTTKSDIYSIGIVFWELVTRVINGEYSRPYSEYSHIKM
DFQIMLNSKEGLRPTLPQNTPL
Translated Amino Acid sequence (All Frames) 
Frame A:
ntnk**y************nkkynnnnsnnsnnnnkiiiieiisi*kinkikkgknkkkn
kfff*KIKXLKKK---

---sfnspck*hwvysvftighlklytetlrv*ifwsmkignakfqilvlvvlippii*k
hsvrfvvplpivhlksp*vmvvstlpnlistvlvssfgn**lvlsmvntlvptvniltsk
wtfksc*tqrkv*dqpylkilhl

Frame B:
tpinnninnnnnnnnnnnkikniiiiiviiviiiik****k*fqfkk*ik*kkekikkki
nfffkklkx*kk---

---lsihhandigctvssqldtsnctqrl*efksfgq*rlgmqsfrfws*sf*yrr*frn
tq*dswylcllft*srrr*w*slhyqi*ylqywyrllgisnscyqw*ilsslq*ifshqn
glsnhvklkgrfktnltskyst

Frame C:
hq*iiiliiiiiiiiiiik*ki*************nnnnrnnfnlknk*nkkrkk*kkk*
ifflkn*xfkkk---

---FQFTMQMTLGVQCLHNWTPQIVHRDFKSLNLLVNEDWECKVSDFGLSRFNTADNLET
LSKIRGTFAYCSPEVAVGNGSLYTTKSDIYSIGIVFWELVTRVINGEYSRPYSEYSHIKM
DFQIMLNSKEGLRPTLPQNTPL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK887 (SHK887Q) /CSM/SH/SHK8-D/SHK887Q.Seq.d/                        833   0.0
SHE857 (SHE857Q) /CSM/SH/SHE8-C/SHE857Q.Seq.d/                        827   0.0
CHS225 (CHS225Q) /CSM/CH/CHS2-B/CHS225Q.Seq.d/                        827   0.0
CHP295 (CHP295Q) /CSM/CH/CHP2-D/CHP295Q.Seq.d/                        827   0.0
AHF423 (AHF423Q) /CSM/AH/AHF4-A/AHF423Q.Seq.d/                        819   0.0
SHF208 (SHF208Q) /CSM/SH/SHF2-A/SHF208Q.Seq.d/                        815   0.0
AHC678 (AHC678Q) /CSM/AH/AHC6-D/AHC678Q.Seq.d/                        813   0.0
SHD120 (SHD120Q) /CSM/SH/SHD1-A/SHD120Q.Seq.d/                        807   0.0
AHJ120 (AHJ120Q) /CSM/AH/AHJ1-A/AHJ120Q.Seq.d/                        759   0.0
SHB434 (SHB434Q) /CSM/SH/SHB4-B/SHB434Q.Seq.d/                        712   0.0
own update 2002.12. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115604|AC115604.2 Dictyostelium discoideum chromosome 2 map 4354771-4414991 strainAX4, complete sequence.    50   2e-08  2
BG646682|BG646682.1 EST508301 HOGA Medicago truncatula cDNA clone pHOGA-9D1 5' end, mRNA sequence.    48   0.26   1
BG645865|BG645865.1 EST507484 KV3 Medicago truncatula cDNA clone pKV3-47F12 5' end, mRNA sequence.    48   0.26   1
BG453581|BG453581.1 NF092A07LF1F1050 Developing leaf Medicago truncatula cDNA clone NF092A07LF 5', mRNA sequence.    48   0.26   1
BG453480|BG453480.1 NF092C08LF1F1055 Developing leaf Medicago truncatula cDNA clone NF092C08LF 5', mRNA sequence.    48   0.26   1
BF647893|BF647893.1 NF038B09EC1F1076 Elicited cell culture Medicago truncatula cDNA clone NF038B09EC 5', mRNA sequence.    48   0.26   1
BF646211|BF646211.1 NF067F06EC1F1058 Elicited cell culture Medicago truncatula cDNA clone NF067F06EC 5', mRNA sequence.    48   0.26   1
BF644581|BF644581.1 NF016B08EC1F1064 Elicited cell culture Medicago truncatula cDNA clone NF016B08EC 5', mRNA sequence.    48   0.26   1
BF642554|BF642554.1 NF060G08IN1F1066 Insect herbivory Medicago truncatula cDNA clone NF060G08IN 5', mRNA sequence.    48   0.26   1
DW018299|DW018299.1 EST1227260 MTY Medicago truncatula cDNA clone MTYB479, mRNA sequence.    48   0.26   1
dna update 2006. 6.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XX5) RecName: Full=Probable serine/threonine-protein kinase ...   289   5e-77
(Q55GG4) RecName: Full=Probable serine/threonine-protein kinase ...   164   3e-39
(Q54G28) RecName: Full=Probable tyrosine-protein kinase DDB_G029...   151   1e-35
(Q54HC6) RecName: Full=Ankyrin repeat-containing protein kinase ...   141   1e-32
(Q54RR9) RecName: Full=Probable serine/threonine-protein kinase ...   136   4e-31
(Q54H46) RecName: Full=Probable serine/threonine-protein kinase ...    93   6e-18
(Q86HG9) RecName: Full=Probable serine/threonine-protein kinase ...    92   2e-17
(Q54H45) RecName: Full=Probable serine/threonine-protein kinase ...    85   2e-15
AC159538_19(AC159538|pid:none) Medicago truncatula clone mth2-48...    84   3e-15
(Q5UQG7) RecName: Full=Putative serine/threonine-protein kinase/...    84   4e-15
protein update 2009. 5.30
PSORT 

 psg: 0.88  gvh: 0.44  alm: 0.40  top: 0.33  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SHK887 is nuc
5' end seq. ID SHK887F
5' end seq. 
>SHK887F.Seq
AACACCAATAAATAATAATATTAATAATAATAATAATAATAATAATAATAATAATAAAAT
AAAAAATATAATAATAATAATAGTAATAATAGTAATAATAATAATAAAATAATAATAATA
GAAATAATTTCAATTTAAAAAATAAATAAAATAAAAAAAGGAAAAAATAAAAAAAAAAAT
AAATTTTTTTTTTAAAAAATTAAAANTTTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 229
3' end seq. ID SHK887Z
3' end seq. 
>SHK887Z.Seq
NNNNNNNNNNTCTTTCAATTCACCATGCAAATGACATTGGGTGTACAGTGTCTTCACAAT
TGGACACCTCAAATTGTACACAGAGACTTTAAGAGTTTAAATCTTTTGGTCAATGAAGAT
TGGGAATGCAAAGTTTCAGATTTTGGTCTTAGTCGTTTTAATACCGCCGATAATTTAGAA
ACACTCAGTAAGATTCGTGGTACCTTTGCCTATTGTTCACCTGAAGTCGCCGTAGGTAAT
GGTAGTCTCTACACTACCAAATCTGATATCTACAGTATTGGTATCGTCTTTTGGGAATTA
GTAACTCGTGTTATCAATGGTGAATACTCTCGTCCCTACAGTGAATATTCTCACATCAAA
ATGGACTTTCAAATCATGTTAAACTCAAAGGAAGGTTTAAGACCAACCTTACCTCAAAAT
ACTCCACTTG
Length of 3' end seq. 430
Connected seq. ID SHK887P
Connected seq. 
>SHK887P.Seq
AACACCAATAAATAATAATATTAATAATAATAATAATAATAATAATAATAATAATAAAAT
AAAAAATATAATAATAATAATAGTAATAATAGTAATAATAATAATAAAATAATAATAATA
GAAATAATTTCAATTTAAAAAATAAATAAAATAAAAAAAGGAAAAAATAAAAAAAAAAAT
AAATTTTTTTTTTAAAAAATTAAAANTTTAAAAAAAAAA----------TCTTTCAATTC
ACCATGCAAATGACATTGGGTGTACAGTGTCTTCACAATTGGACACCTCAAATTGTACAC
AGAGACTTTAAGAGTTTAAATCTTTTGGTCAATGAAGATTGGGAATGCAAAGTTTCAGAT
TTTGGTCTTAGTCGTTTTAATACCGCCGATAATTTAGAAACACTCAGTAAGATTCGTGGT
ACCTTTGCCTATTGTTCACCTGAAGTCGCCGTAGGTAATGGTAGTCTCTACACTACCAAA
TCTGATATCTACAGTATTGGTATCGTCTTTTGGGAATTAGTAACTCGTGTTATCAATGGT
GAATACTCTCGTCCCTACAGTGAATATTCTCACATCAAAATGGACTTTCAAATCATGTTA
AACTCAAAGGAAGGTTTAAGACCAACCTTACCTCAAAATACTCCACTTG
Length of connected seq. 639
Full length Seq ID -
Full length Seq. -
Length of full length seq. -