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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK869
Library SH
(Link to library)
Clone ID SHK869
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16313-1
Original site URL
Representative seq. ID SHK869P
(Link to Original site)
Representative DNA sequence 
>SHK869 (SHK869Q) /CSM/SH/SHK8-C/SHK869Q.Seq.d/
TAAAATAATAACAATAAAATAGATTTTTACATTTATTTTTATTTATTTCATATTTATTTT
TGTTGGAAAAAAAAGAAAAAAAAAGAAAAAAAGAAAAAAAAAAXXXXXXXXXXTTTTAAA
TTGTGATGATAATAACGATTGTACTGAAGATAAGTGTTTAAATACTGGTATTTGTCAAAA
TAATCCAATTGACGGTTGTGTTACACCATCACCAGTTTCAATTGGTGGAACTATCCCTCC
AACCACTACTGGTGTATCACAAGTTGAGTGTGATTGTTGTCCAATTGGTCAAAAATGTTT
ATTAGTCAATGGTCATGAAATTTGTATTAAACCAGCAACCACTGGTGGTATTCCAGAATT
AACTACTGGTTGTGCTGGTACAACAACTGGCAGGGCTACAGGTCATTACACAGAATCAGG
TACAGGTAATCCACATTTATGTGATAGATATCATTGTAAAAGAGGAATGGAATGTCATGT
TGTTAATGGTGTTCCAGAATGTTTACCATCAAACTACAAATGTTTAGATTGTTTAGATCT
TCATTGTGAGAGACAAGGTGATTTCACCTGTTTTATGGTTAAAAATCCAAATTTCATTTC
TAGTAAACATAGTTGTTATGGTGAATCATGTTGTAAATTCACACCA
sequence update 2002.10.25
Translated Amino Acid sequence 
*NNNNKIDFYIYFYLFHIYFCWKKKKKKEKKKKK---

---LNCDDNNDCTEDKCLNTGICQNNPIDGCVTPSPVSIGGTIPPTTTGVSQVECDCCPI
GQKCLLVNGHEICIKPATTGGIPELTTGCAGTTTGRATGHYTESGTGNPHLCDRYHCKRG
MECHVVNGVPECLPSNYKCLDCLDLHCERQGDFTCFMVKNPNFISSKHSCYGESCCKFTP
Translated Amino Acid sequence (All Frames) 
Frame A:
*NNNNKIDFYIYFYLFHIYFCWKKKKKKEKKKKK---

---f*ivmiitivlkisv*ilvfvkiiqltvvlhhhqfqlvelslqpllvyhklsvivvq
lvknvy*smvmkfvlnqqplvvfqn*llvvlvqqlaglqvitqnqvqvihiyvidiivke
ewnvmllmvfqnvyhqttnv*iv*ifivrdkvispvlwlkiqisflvnivvmvnhvvnsh

Frame B:
kiitik*iftfifiyfififvgkkrkkkkkrkkk---

---fkl****rly*r*vfkywylsk*sn*rlcytitsfnwwnypsnhywcits*v*llsn
wskmfisqws*nly*tsnhwwysrinywlcwynnwqgyrslhriryr*stfm**isl*kr
ngmscc*wcsrmftiklqmfrlfrssl*etr*fhlfyg*kskfhf**t*llw*iml*iht

Frame C:
k**q*nrflhlflfisylfllekkekkrkkekk---

---LNCDDNNDCTEDKCLNTGICQNNPIDGCVTPSPVSIGGTIPPTTTGVSQVECDCCPI
GQKCLLVNGHEICIKPATTGGIPELTTGCAGTTTGRATGHYTESGTGNPHLCDRYHCKRG
MECHVVNGVPECLPSNYKCLDCLDLHCERQGDFTCFMVKNPNFISSKHSCYGESCCKFTP
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHL803 (SHL803Q) /CSM/SH/SHL8-A/SHL803Q.Seq.d/                       1057   0.0
SHL457 (SHL457Q) /CSM/SH/SHL4-C/SHL457Q.Seq.d/                       1057   0.0
SHL385 (SHL385Q) /CSM/SH/SHL3-D/SHL385Q.Seq.d/                       1057   0.0
SHL169 (SHL169Q) /CSM/SH/SHL1-C/SHL169Q.Seq.d/                       1057   0.0
SHK884 (SHK884Q) /CSM/SH/SHK8-D/SHK884Q.Seq.d/                       1057   0.0
SHK869 (SHK869Q) /CSM/SH/SHK8-C/SHK869Q.Seq.d/                       1057   0.0
SHJ893 (SHJ893Q) /CSM/SH/SHJ8-D/SHJ893Q.Seq.d/                       1057   0.0
SHJ886 (SHJ886Q) /CSM/SH/SHJ8-D/SHJ886Q.Seq.d/                       1057   0.0
SHJ707 (SHJ707Q) /CSM/SH/SHJ7-A/SHJ707Q.Seq.d/                       1057   0.0
SHJ668 (SHJ668Q) /CSM/SH/SHJ6-C/SHJ668Q.Seq.d/                       1057   0.0
own update 2002.12. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC020547|AC020547.7 Homo sapiens BAC clone RP11-134F3 from 2, complete sequence.    46   1.0    1
AL161506|AL161506.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 18.    42   1.4    3
AC168497|AC168497.2 Strongylocentrotus purpuratus clone R3-3117D9, WORKING DRAFT SEQUENCE, 23 unordered pieces.    34   2.0    2
CV752175|CV752175.1 SJL6-009_A06_SJL6-009A06-T3_039.ab1 SJL Schistosoma japonicum cDNA, mRNA sequence.    44   4.0    1
CV752026|CV752026.1 SJL6-007_B08_SJL6-007B08-T3_063.ab1 SJL Schistosoma japonicum cDNA, mRNA sequence.    44   4.0    1
CV739281|CV739281.1 SJAL_019_73.T3 SJL Schistosoma japonicum cDNA, mRNA sequence.    44   4.0    1
CV739266|CV739266.1 SJAL_019_64.T3 SJL Schistosoma japonicum cDNA, mRNA sequence.    44   4.0    1
CV682259|CV682259.1 sjs6-009_H10_sjs6-009H10-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    44   4.0    1
CV581768|CV581768.1 sjmi-1-002_E12_sjmi-1-002E12-T3 Schistosoma japonicum miracidium cDNA Schistosoma japonicum cDNA, mRNA sequence.    44   4.0    1
BU725144|BU725144.1 SJMBXF01 SJM Schistosoma japonicum cDNA, mRNA sequence.    44   4.0    1
dna update 2006. 6.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                307   2e-82
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                182   9e-45
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    52   1e-05
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    42   0.015
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    41   0.033
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    41   0.033
AB011475_1(AB011475|pid:none) Arabidopsis thaliana genomic DNA, ...    37   0.36
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    37   0.48
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    37   0.62
(Q54FD8) RecName: Full=Protein psiD; Flags: Precursor;                 35   1.4
protein update 2009. 1.17
PSORT 

 psg: 0.91  gvh: 0.37  alm: 0.47  top: 0.43  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.20  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SHK869 is nuc
5' end seq. ID SHK869F
5' end seq. 
>SHK869F.Seq
TAAAATAATAACAATAAAATAGATTTTTACATTTATTTTTATTTATTTCATATTTATTTT
TGTTGGAAAAAAAAGAAAAAAAAAGAAAAAAAGAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 113
3' end seq. ID SHK869Z
3' end seq. 
>SHK869Z.Seq
NNNNNNNNNNTTTTAAATTGTGATGATAATAACGATTGTACTGAAGATAAGTGTTTAAAT
ACTGGTATTTGTCAAAATAATCCAATTGACGGTTGTGTTACACCATCACCAGTTTCAATT
GGTGGAACTATCCCTCCAACCACTACTGGTGTATCACAAGTTGAGTGTGATTGTTGTCCA
ATTGGTCAAAAATGTTTATTAGTCAATGGTCATGAAATTTGTATTAAACCAGCAACCACT
GGTGGTATTCCAGAATTAACTACTGGTTGTGCTGGTACAACAACTGGCAGGGCTACAGGT
CATTACACAGAATCAGGTACAGGTAATCCACATTTATGTGATAGATATCATTGTAAAAGA
GGAATGGAATGTCATGTTGTTAATGGTGTTCCAGAATGTTTACCATCAAACTACAAATGT
TTAGATTGTTTAGATCTTCATTGTGAGAGACAAGGTGATTTCACCTGTTTTATGGTTAAA
AATCCAAATTTCATTTCTAGTAAACATAGTTGTTATGGTGAATCATGTTGTAAATTCACA
CCA
Length of 3' end seq. 543
Connected seq. ID SHK869P
Connected seq. 
>SHK869P.Seq
TAAAATAATAACAATAAAATAGATTTTTACATTTATTTTTATTTATTTCATATTTATTTT
TGTTGGAAAAAAAAGAAAAAAAAAGAAAAAAAGAAAAAAAAAA----------TTTTAAA
TTGTGATGATAATAACGATTGTACTGAAGATAAGTGTTTAAATACTGGTATTTGTCAAAA
TAATCCAATTGACGGTTGTGTTACACCATCACCAGTTTCAATTGGTGGAACTATCCCTCC
AACCACTACTGGTGTATCACAAGTTGAGTGTGATTGTTGTCCAATTGGTCAAAAATGTTT
ATTAGTCAATGGTCATGAAATTTGTATTAAACCAGCAACCACTGGTGGTATTCCAGAATT
AACTACTGGTTGTGCTGGTACAACAACTGGCAGGGCTACAGGTCATTACACAGAATCAGG
TACAGGTAATCCACATTTATGTGATAGATATCATTGTAAAAGAGGAATGGAATGTCATGT
TGTTAATGGTGTTCCAGAATGTTTACCATCAAACTACAAATGTTTAGATTGTTTAGATCT
TCATTGTGAGAGACAAGGTGATTTCACCTGTTTTATGGTTAAAAATCCAAATTTCATTTC
TAGTAAACATAGTTGTTATGGTGAATCATGTTGTAAATTCACACCA
Length of connected seq. 636
Full length Seq ID -
Full length Seq. -
Length of full length seq. -