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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK720
Library SH
(Link to library)
Clone ID SHK720
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12076-1
Original site URL
Representative seq. ID SHK720P
(Link to Original site)
Representative DNA sequence 
>SHK720 (SHK720Q) /CSM/SH/SHK7-A/SHK720Q.Seq.d/
TGAAAAAAGTAAATATAATAAACGTTACAATGAACCAAATGATCCTTTAGAAATCGATTA
TATGAGAGGTTATCAAACAACCACACTCTCAAATATTTTACATTATGGTTTAATTATATT
ATCAGCAGGTTTATTATTATTAGTTTATCATTGGGTACCAAGATGGGGTATTAAAATGAG
ATTTAAAGAATGTAAATTAAATGATGCAAATTATGTTTATGTTAAAGCAAAAXXXXXXXX
XXCTATTGTATTTCGATGGTTCCGTATTGAATAACTATCAATATCTCTATCAAGATCTTT
GGGTAATTTTCCCATTGGTAATTTTCATGGGTCGTACTGAACCATGTAGTAAACTCTCCA
TCAAAAGACCATCCTCTCGTTTAATTAGTTCAACAGTTATTAGTAGTTTACTCAGTCATG
TCACATTAACATTTGTTTTCCAATTATTGGTTTACATTTTCGTACGTCGTAATTCATGGT
ATAAGGATTTACCCGACACAAGCTCCAATGAAAATGAACCAAGATTAGATCATTTATTGG
CATCCCTTTTCATCCTATGGTAATATGCAATACTCTGTCATGTGTTTCATTTACTCTTGG
GGTAAACCTTTCCTTAGACCAATTTATACCAATCGTGCATTATTCATCTTCTTTTGTGTA
ACACTTACAACTTCTTCATTATTATTATTGTTGCCTGTTTCCATTAGATTCTTTGGTACA
ACATGGTTAATGGATTTACCAGTACGTTGGAGAGTTGAACTCTTAATTCTCTTTATTATC
AATGCTGCTCTCGCTATGGCTGCCGAATGGATTATCATTGTCTATCGTGAAATCAGATCA
AAATCAAAGAGAAAATTAAAACAAAAACAAAATTCCGATCCAAATATTATTGCAAAAAAT
ACTGTTAATGAAAGATATACTAGTTTAAATTAAATTTTAGTATTTAAAAAATTATTAAAC
AGTATAATAATAAATAAATAAAATAAAATAAAAAAAAACAATAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
EKSKYNKRYNEPNDPLEIDYMRGYQTTTLSNILHYGLIILSAGLLLLVYHWVPRWGIKMR
FKECKLNDANYVYVKAK---

---LLYFDGSVLNNYQYLYQDLWVIFPLVIFMGRTEPCSKLSIKRPSSRLISSTVISSLL
SHVTLTFVFQLLVYIFVRRNSWYKDLPDTSSNENEPRLDHLLASLFILW*yailchvfhl
llg*tfp*tnlyqsciihlllcntynffiiiivacfh*ilwynmvngftstles*tlnsl
yyqccsrygcrmdyhcls*nqikikekiktktkfrskyyckkyc**kiy*fklnfsi*ki
ikqynnk*ik*nkkkq*
Translated Amino Acid sequence (All Frames) 
Frame A:
*kk*i**tlq*tk*sfrnrlyerlsnnhtlkyftlwfnyiisrfiiislslgtkmgy*ne
i*rm*ik*cklclc*sk---

---LLYFDGSVLNNYQYLYQDLWVIFPLVIFMGRTEPCSKLSIKRPSSRLISSTVISSLL
SHVTLTFVFQLLVYIFVRRNSWYKDLPDTSSNENEPRLDHLLASLFILW*yailchvfhl
llg*tfp*tnlyqsciihlllcntynffiiiivacfh*ilwynmvngftstles*tlnsl
yyqccsrygcrmdyhcls*nqikikekiktktkfrskyyckkyc**kiy*fklnfsi*ki
ikqynnk*ik*nkkkq*

Frame B:
EKSKYNKRYNEPNDPLEIDYMRGYQTTTLSNILHYGLIILSAGLLLLVYHWVPRWGIKMR
FKECKLNDANYVYVKAK---

---ycismvpy*itinisikifg*fshw*fswvvlnhvvnspskdhplv*lvqqllvvys
vmsh*hlfsnywftfsyvvihgiriyptqapmkmnqd*iiywhpfssygnmqysvmcfiy
swgkpflrpiytnralfiffcvtlttsslllllpvsirffgttwlmdlpvrwrvellilf
iinaalamaaewiiivyreirskskrklkqkqnsdpniiakntvnerytsln*ilvfkkl
lnsiiink*nkikknnk

Frame C:
kkvniinvtmnqmil*ksii*evikqphsqifyimv*lyyqqvyyy*fiigyqdgvlk*d
lknvn*mmqimfmlkq---

---ivfrwfrie*lsislsrslgnfpignfhgsy*tm**tlhqktilsfn*fnsy**ftq
schinicfpiiglhfrts*fmv*gftrhklq*k*tkirsfigipfhpmvicntlscvsft
lgvnlsldqfipivhysssfv*hlqllhyyycclfpldslvqhg*wiyqyvgelns*fsl
lsmllslwlpnglslsivksdqnqren*nknkipiqillqkillmkdilv*ikf*ylkny
*tv***inkik*kktik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK720 (SHK720Q) /CSM/SH/SHK7-A/SHK720Q.Seq.d/                       1600   0.0
VHL407 (VHL407Q) /CSM/VH/VHL4-A/VHL407Q.Seq.d/                       1166   0.0
VHB236 (VHB236Q) /CSM/VH/VHB2-B/VHB236Q.Seq.d/                       1166   0.0
VHA610 (VHA610Q) /CSM/VH/VHA6-A/VHA610Q.Seq.d/                       1166   0.0
SHL369 (SHL369Q) /CSM/SH/SHL3-C/SHL369Q.Seq.d/                       1142   0.0
SHF178 (SHF178Q) /CSM/SH/SHF1-D/SHF178Q.Seq.d/                       1138   0.0
VHA543 (VHA543Q) /CSM/VH/VHA5-B/VHA543Q.Seq.d/                       1094   0.0
AFK790 (AFK790Q) /CSM/AF/AFK7-D/AFK790Q.Seq.d/                         42   0.016
VSE612 (VSE612Q) /CSM/VS/VSE6-A/VSE612Q.Seq.d/                         40   0.064
AFL661 (AFL661Q) /CSM/AF/AFL6-C/AFL661Q.Seq.d/                         40   0.064
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CZ538514|CZ538514.1 SRAA-aad20b09.b1 Strongyloides ratti whole genome shotgun library(SRAAGSS 004) Strongyloides ratti genomic, genomic survey sequence.    36   0.058  3
BX546461|BX546461.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-274O20.    40   0.20   6
AE014841|AE014841.1 Plasmodium falciparum 3D7 chromosome 11 section 6 of 8 of the complete sequence.    38   0.20   7
AC179856|AC179856.1 Strongylocentrotus purpuratus clone R3-1007I16, WORKING DRAFT SEQUENCE, 22 unordered pieces.    36   0.38   5
EC313449|EC313449.1 REPROTRACTF081141I8 POSSUM_01-POSSUM-C-REPROTRACT-2KB Trichosurus vulpecula cDNA clone 1061023012758, mRNA sequence.    48   0.41   1
AC131622|AC131622.5 Rattus norvegicus clone CH230-117E17, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48   0.41   1
AC110324|AC110324.7 Rattus norvegicus clone CH230-252M1, WORKING DRAFT SEQUENCE, 1 ordered piece.    48   0.41   1
AC105652|AC105652.4 Rattus norvegicus clone CH230-116K15, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48   0.41   1
CT573147|CT573147.7 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-240H12.    34   0.50   6
CR626903|CR626903.5 Zebrafish DNA sequence from clone CH211-262A22 in linkage group 16.    36   0.82   6
dna update 2006. 6.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FM992689_210(FM992689|pid:none) Candida dubliniensis CD36 chromo...    59   3e-07
AE016819_800(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...    55   1e-06
CR380959_492(CR380959|pid:none) Candida glabrata strain CBS138 c...    50   2e-05
(Q21286) RecName: Full=Probable cation-transporting ATPase K07E3...    45   8e-05
U41552_7(U41552|pid:none) Caenorhabditis elegans cosmid K07E3, c...    45   8e-05
CR382130_932(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...    47   0.001
AE014135_23(AE014135|pid:none) Drosophila melanogaster chromosom...    32   0.008
CU640366_567(CU640366|pid:none) Podospora anserina genomic DNA c...    39   0.21
(Q4VNC0) RecName: Full=Probable cation-transporting ATPase 13A5;...    39   0.27
AB072766_1(AB072766|pid:none) Macaca fascicularis testis cDNA cl...    39   0.35
protein update 2009. 5.30
PSORT 

 psg: 0.49  gvh: 0.55  alm: 0.24  top: 0.67  tms: 0.13  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: plasma membrane
	 16.0 %: nuclear
	 16.0 %: vacuolar
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for SHK720 is end
5' end seq. ID SHK720F
5' end seq. 
>SHK720F.Seq
TGAAAAAAGTAAATATAATAAACGTTACAATGAACCAAATGATCCTTTAGAAATCGATTA
TATGAGAGGTTATCAAACAACCACACTCTCAAATATTTTACATTATGGTTTAATTATATT
ATCAGCAGGTTTATTATTATTAGTTTATCATTGGGTACCAAGATGGGGTATTAAAATGAG
ATTTAAAGAATGTAAATTAAATGATGCAAATTATGTTTATGTTAAAGCAAAANNNNNNNN
NN
Length of 5' end seq. 242
3' end seq. ID SHK720Z
3' end seq. 
>SHK720Z.Seq
NNNNNNNNNNCTATTGTATTTCGATGGTTCCGTATTGAATAACTATCAATATCTCTATCA
AGATCTTTGGGTAATTTTCCCATTGGTAATTTTCATGGGTCGTACTGAACCATGTAGTAA
ACTCTCCATCAAAAGACCATCCTCTCGTTTAATTAGTTCAACAGTTATTAGTAGTTTACT
CAGTCATGTCACATTAACATTTGTTTTCCAATTATTGGTTTACATTTTCGTACGTCGTAA
TTCATGGTATAAGGATTTACCCGACACAAGCTCCAATGAAAATGAACCAAGATTAGATCA
TTTATTGGCATCCCTTTTCATCCTATGGTAATATGCAATACTCTGTCATGTGTTTCATTT
ACTCTTGGGGTAAACCTTTCCTTAGACCAATTTATACCAATCGTGCATTATTCATCTTCT
TTTGTGTAACACTTACAACTTCTTCATTATTATTATTGTTGCCTGTTTCCATTAGATTCT
TTGGTACAACATGGTTAATGGATTTACCAGTACGTTGGAGAGTTGAACTCTTAATTCTCT
TTATTATCAATGCTGCTCTCGCTATGGCTGCCGAATGGATTATCATTGTCTATCGTGAAA
TCAGATCAAAATCAAAGAGAAAATTAAAACAAAAACAAAATTCCGATCCAAATATTATTG
CAAAAAATACTGTTAATGAAAGATATACTAGTTTAAATTAAATTTTAGTATTTAAAAAAT
TATTAAACAGTATAATAATAAATAAATAAAATAAAATAAAAAAAAACAATAAAA
Length of 3' end seq. 774
Connected seq. ID SHK720P
Connected seq. 
>SHK720P.Seq
TGAAAAAAGTAAATATAATAAACGTTACAATGAACCAAATGATCCTTTAGAAATCGATTA
TATGAGAGGTTATCAAACAACCACACTCTCAAATATTTTACATTATGGTTTAATTATATT
ATCAGCAGGTTTATTATTATTAGTTTATCATTGGGTACCAAGATGGGGTATTAAAATGAG
ATTTAAAGAATGTAAATTAAATGATGCAAATTATGTTTATGTTAAAGCAAAA--------
--CTATTGTATTTCGATGGTTCCGTATTGAATAACTATCAATATCTCTATCAAGATCTTT
GGGTAATTTTCCCATTGGTAATTTTCATGGGTCGTACTGAACCATGTAGTAAACTCTCCA
TCAAAAGACCATCCTCTCGTTTAATTAGTTCAACAGTTATTAGTAGTTTACTCAGTCATG
TCACATTAACATTTGTTTTCCAATTATTGGTTTACATTTTCGTACGTCGTAATTCATGGT
ATAAGGATTTACCCGACACAAGCTCCAATGAAAATGAACCAAGATTAGATCATTTATTGG
CATCCCTTTTCATCCTATGGTAATATGCAATACTCTGTCATGTGTTTCATTTACTCTTGG
GGTAAACCTTTCCTTAGACCAATTTATACCAATCGTGCATTATTCATCTTCTTTTGTGTA
ACACTTACAACTTCTTCATTATTATTATTGTTGCCTGTTTCCATTAGATTCTTTGGTACA
ACATGGTTAATGGATTTACCAGTACGTTGGAGAGTTGAACTCTTAATTCTCTTTATTATC
AATGCTGCTCTCGCTATGGCTGCCGAATGGATTATCATTGTCTATCGTGAAATCAGATCA
AAATCAAAGAGAAAATTAAAACAAAAACAAAATTCCGATCCAAATATTATTGCAAAAAAT
ACTGTTAATGAAAGATATACTAGTTTAAATTAAATTTTAGTATTTAAAAAATTATTAAAC
AGTATAATAATAAATAAATAAAATAAAATAAAAAAAAACAATAAAA
Length of connected seq. 996
Full length Seq ID -
Full length Seq. -
Length of full length seq. -