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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK686
Library SH
(Link to library)
Clone ID SHK686
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14926-1
Original site URL
Representative seq. ID SHK686P
(Link to Original site)
Representative DNA sequence 
>SHK686 (SHK686Q) /CSM/SH/SHK6-D/SHK686Q.Seq.d/
ATTACAACTCAAACATCCACCATCACTTTTTATAGTATTACTACTTTAAAATTTGACCTA
TTTTTTATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAAGGGGATTTAAAAAA
GGAATCATTTTTTTTCACTCTTTATTTTGTACACTATAAAAAAAAAAXXXXXXXXXXGAT
TCAGGTGATGGTGTCACCCAATATCATTCCAGTCTATGAGGGTTACTCAATCCCACATTT
GACTCGTCGTCTCGATGTTGCCGGTCGTGATGTAACTCGTTACCTCATCAAATTATTATT
ACTCCGTGGTTACGCTTTCAATCGTACCGCCGATTTCGAAACCATCCGTCAAATCAAAGA
GAAACTCTGTTATGTCGCATACGATGTTCAACAAGAGATGAAATTGGCCTCAGAGACCAC
TGTTCTCGTCGAAAACTACACCCTCCCAGACGGTCGTGTAATCAAAGTTGGCCAAGAGAG
ATTCCAAGCCTCTGAAGCCCTCTTCAATCCATCTTTGGTCGATGTCGAAGGTGGTGGTGT
CCATGAACAATTGTTTGATTGTATCACCAAGGCAGACCGTGATCTTCAACAAGGTTTCTA
TCAACATATCGTCCTTTCTGGTGGTTCCTCTATGTATCCAGGTTTACCATCTCGTCTCGA
AAAGGAAATTAGATCTCTCTATA
sequence update 2002.10.25
Translated Amino Acid sequence 
lqlkhppslfivlll*nltyflfiklnnsl*ir**GDLKKESFFFTLYFVHYKKK---

---IQVMVSPNIIPVYEGYSIPHLTRRLDVAGRDVTRYLIKLLLLRGYAFNRTADFETIR
QIKEKLCYVAYDVQQEMKLASETTVLVENYTLPDGRVIKVGQERFQASEALFNPSLVDVE
GGGVHEQLFDCITKADRDLQQGFYQHIVLSGGSSMYPGLPSRLEKEIRSLY
Translated Amino Acid sequence (All Frames) 
Frame A:
ittqtstitfysittlkfdlffiy*ik*fivnsiirgfkkgiiffhslfctl*kk---

---dsgdgvtqyhssl*gllnptfdsssrccrs*cnslphqiiitpwlrfqsyrrfrnhp
snqretllcrircstrdeiglrdhcsrrklhpprrscnqswpreipsl*splqsifgrcr
rwwcp*tiv*lyhqgrp*sstrflstyrpfwwflyvsrftissrkgn*isl

Frame B:
lqlkhppslfivlll*nltyflfiklnnsl*ir**GDLKKESFFFTLYFVHYKKK---

---IQVMVSPNIIPVYEGYSIPHLTRRLDVAGRDVTRYLIKLLLLRGYAFNRTADFETIR
QIKEKLCYVAYDVQQEMKLASETTVLVENYTLPDGRVIKVGQERFQASEALFNPSLVDVE
GGGVHEQLFDCITKADRDLQQGFYQHIVLSGGSSMYPGLPSRLEKEIRSLY

Frame C:
ynsnihhhfl*yyyfki*pifylln*iihckfdnkgi*krnhffslfilytikkk---

---fr*wchpisfqsmrvtqshi*lvvsmlpvvm*lvtssnyyysvvtlsivppiskpsv
kskrnsvmshtmfnkr*nwpqrplfsskttpsqtvv*sklakrdskplkpssihlwsmsk
vvvsmnnclivsprqtvifnkvsinissflvvplciqvyhlvskrkldlsi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK686 (SHK686Q) /CSM/SH/SHK6-D/SHK686Q.Seq.d/                       1003   0.0
SLB194 (SLB194Q) /CSM/SL/SLB1-D/SLB194Q.Seq.d/                        985   0.0
FC-AH01 (FC-AH01Q) /CSM/FC/FC-AH/FC-AH01Q.Seq.d/                      985   0.0
FC-131 (FC-131Q) /CSM/CG/CG-FC/FC-131Q.Seq.d/                         985   0.0
AFC587 (AFC587Q) /CSM/AF/AFC5-D/AFC587Q.Seq.d/                        985   0.0
AFM162 (AFM162Q) /CSM/AF/AFM1-C/AFM162Q.Seq.d/                        948   0.0
VFD448 (VFD448Q) /CSM/VF/VFD4-B/VFD448Q.Seq.d/                        934   0.0
AFJ309 (AFJ309Q) /CSM/AF/AFJ3-A/AFJ309Q.Seq.d/                        934   0.0
CFH233 (CFH233Q) /CSM/CF/CFH2-B/CFH233Q.Seq.d/                        930   0.0
AFJ315 (AFJ315Q) /CSM/AF/AFJ3-A/AFJ315Q.Seq.d/                        926   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF095929|AF095929.1 Dictyostelium discoideum actin related protein 2 (arpB) mRNA, complete cds.   955   0.0    4
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   955   0.0    4
DR701670|DR701670.1 Asn_01165 Aspergillus niger pBluescript (EcoRI-XhoI) Aspergillus niger cDNA clone Asn_01165, mRNA sequence.    50   2e-08  2
U29609|U29609.1 Acanthamoeba castellanii actin-related protein 2 Arp2 mRNA, complete cds.    50   9e-08  2
EC110707|EC110707.1 ACE00019710 1:1 combination of non-normalized and normalized-small libraries Acanthamoeba castellanii cDNA similar to actin-related protein 2 Arp2 - highly similar to published Acanthamoeba castellanii sequence, mRNA sequence.    50   9e-08  2
EC108807|EC108807.1 ACE00016870 1:1 combination of non-normalized and normalized-small libraries Acanthamoeba castellanii cDNA similar to actin-related protein 2 Arp2 - highly similar to published Acanthamoeba castellanii sequence, mRNA sequence.    50   9e-08  2
EB027420|EB027420.1 LscoSEQ7816 Leucosporidium scottii pBluescript (EcoRI-XhoI) Leucosporidium scottii cDNA clone LscoSEQ7816, mRNA sequence.    54   3e-07  2
EB021565|EB021565.1 LscoSEQ13808 Leucosporidium scottii pBluescript (EcoRI-XhoI) Leucosporidium scottii cDNA clone LscoSEQ13808, mRNA sequence.    54   3e-07  2
EB020998|EB020998.1 LscoSEQ13129 Leucosporidium scottii pBluescript (EcoRI-XhoI) Leucosporidium scottii cDNA clone LscoSEQ13129, mRNA sequence.    54   3e-07  2
EB018390|EB018390.1 LscoSEQ10064 Leucosporidium scottii pBluescript (EcoRI-XhoI) Leucosporidium scottii cDNA clone LscoSEQ10064, mRNA sequence.    54   3e-07  2
dna update 2006. 6.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O96621) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   320   2e-86
(P53487) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   249   7e-65
BC047530_1(BC047530|pid:none) Mus musculus ARP2 actin-related pr...   246   3e-64
(Q5BL41) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   246   3e-64
AY891099_1(AY891099|pid:none) Synthetic construct Homo sapiens c...   246   3e-64
(Q5M7U6) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   246   3e-64
BC036253_1(BC036253|pid:none) Homo sapiens ARP2 actin-related pr...   246   3e-64
(P53488) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   246   3e-64
(Q7ZTP2) RecName: Full=Actin-related protein 2-A; AltName: Full=...   246   3e-64
(Q7ZXV3) RecName: Full=Actin-related protein 2-B; AltName: Full=...   245   8e-64
protein update 2009. 5.30
PSORT 

 psg: 0.97  gvh: 0.33  alm: 0.49  top: 0.70  tms: 0.00  mit: 0.17  mip: 0.10
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for SHK686 is nuc
5' end seq. ID SHK686F
5' end seq. 
>SHK686F.Seq
ATTACAACTCAAACATCCACCATCACTTTTTATAGTATTACTACTTTAAAATTTGACCTA
TTTTTTATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAAGGGGATTTAAAAAA
GGAATCATTTTTTTTCACTCTTTATTTTGTACACTATAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 177
3' end seq. ID SHK686Z
3' end seq. 
>SHK686Z.Seq
NNNNNNNNNNGATTCAGGTGATGGTGTCACCCAATATCATTCCAGTCTATGAGGGTTACT
CAATCCCACATTTGACTCGTCGTCTCGATGTTGCCGGTCGTGATGTAACTCGTTACCTCA
TCAAATTATTATTACTCCGTGGTTACGCTTTCAATCGTACCGCCGATTTCGAAACCATCC
GTCAAATCAAAGAGAAACTCTGTTATGTCGCATACGATGTTCAACAAGAGATGAAATTGG
CCTCAGAGACCACTGTTCTCGTCGAAAACTACACCCTCCCAGACGGTCGTGTAATCAAAG
TTGGCCAAGAGAGATTCCAAGCCTCTGAAGCCCTCTTCAATCCATCTTTGGTCGATGTCG
AAGGTGGTGGTGTCCATGAACAATTGTTTGATTGTATCACCAAGGCAGACCGTGATCTTC
AACAAGGTTTCTATCAACATATCGTCCTTTCTGGTGGTTCCTCTATGTATCCAGGTTTAC
CATCTCGTCTCGAAAAGGAAATTAGATCTCTCTATA
Length of 3' end seq. 516
Connected seq. ID SHK686P
Connected seq. 
>SHK686P.Seq
ATTACAACTCAAACATCCACCATCACTTTTTATAGTATTACTACTTTAAAATTTGACCTA
TTTTTTATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAAGGGGATTTAAAAAA
GGAATCATTTTTTTTCACTCTTTATTTTGTACACTATAAAAAAAAAA----------GAT
TCAGGTGATGGTGTCACCCAATATCATTCCAGTCTATGAGGGTTACTCAATCCCACATTT
GACTCGTCGTCTCGATGTTGCCGGTCGTGATGTAACTCGTTACCTCATCAAATTATTATT
ACTCCGTGGTTACGCTTTCAATCGTACCGCCGATTTCGAAACCATCCGTCAAATCAAAGA
GAAACTCTGTTATGTCGCATACGATGTTCAACAAGAGATGAAATTGGCCTCAGAGACCAC
TGTTCTCGTCGAAAACTACACCCTCCCAGACGGTCGTGTAATCAAAGTTGGCCAAGAGAG
ATTCCAAGCCTCTGAAGCCCTCTTCAATCCATCTTTGGTCGATGTCGAAGGTGGTGGTGT
CCATGAACAATTGTTTGATTGTATCACCAAGGCAGACCGTGATCTTCAACAAGGTTTCTA
TCAACATATCGTCCTTTCTGGTGGTTCCTCTATGTATCCAGGTTTACCATCTCGTCTCGA
AAAGGAAATTAGATCTCTCTATA
Length of connected seq. 673
Full length Seq ID -
Full length Seq. -
Length of full length seq. -