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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ723
Library AF
(Link to library)
Clone ID AFJ723
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10768-1
Original site URL
Representative seq. ID AFJ723P
(Link to Original site)
Representative DNA sequence 
>AFJ723 (AFJ723Q) /CSM/AF/AFJ7-A/AFJ723Q.Seq.d/
ATAATTTTATATATTTGAATACATAATTTATATTTATTATTTTATAAATATATATTTTTA
ATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAGAAGAAG
AAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAGTTACAG
TTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTATTAATG
CTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTTATGATC
CAGGCTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTGATAAAG
GAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACTTTTTAG
AGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATGGTTGGA
GTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGAAAACAT
TTAGATATGATGCTCATCCAATXXXXXXXXXXCCAGTTTTATGTCGTGCATTGGAAATTT
TATTCATTCTTCATGCAGATCATGAATTAAATTGTTCAACCGCTGCAATGAGACATATTT
CATCATCAGGTACCGATCCATACACAAGTGTTGCTGGTGCCGCTGGTGCATTATATGGTC
CATCTCATGGTGGTGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTCCAAAG
AGAATATTCCACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCG
GTCATAGAATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATG
AAGTTTTCGAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAAC
AAGCCTTGGAAGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACT
CTGGTCTCATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTA
TTCCACGTGCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTA
AAATCTATCGTCCACGTCAAGTTTACAAAGGTGAAGGTTTAGAAACTATGTCCCAATCGA
TGGTAGACCACCAGCTAAAGTTCGTTCTCAGGATAGTTATTCGTCTGCTACCACTAAAAG
A
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nfiylnt*fifiil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETVTV
TDNRNGKSYDFKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGDKG
ILRYRGYPIEELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENLVGLMKTF
RYDAHP---

---PVLCRALEILFILHADHELNCSTAAMRHISSSGTDPYTSVAGAAGALYGPSHGGANE
AVLDMLIHIGSKENIPQFISDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEVFESLGK
EPLIEVATELEKQALEDEYFVSRKLYPNVDFYSGLIYKAMGFPTDMFPVLFTIPRAVGWL
AHWVEHLEDPETKIYRPRQVYKGEGLETMSQSMVDHQLKFVLRIVIRLLPLK
Translated Amino Acid sequence (All Frames) 
Frame A:
iilyi*ihnlyllfykyifliwhilivliylpiifqrkkkkkmny*lhqfllikkkrqlq
lqiiemvkamisklkmtllml*tlknyn*pkvmvv**fmiqaskiqql*hhtlhilmvik
ef*dteviqlkn*qkdqtf*rllif*smvtyqinhn*mvgvikl*lilffmkiwlvl*kh
ldmmliq---

---PVLCRALEILFILHADHELNCSTAAMRHISSSGTDPYTSVAGAAGALYGPSHGGANE
AVLDMLIHIGSKENIPQFISDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEVFESLGK
EPLIEVATELEKQALEDEYFVSRKLYPNVDFYSGLIYKAMGFPTDMFPVLFTIPRAVGWL
AHWVEHLEDPETKIYRPRQVYKGEGLETMSQSMVDHQLKFVLRIVIRLLPLK

Frame B:
*fyifeyiiyiyyfiniyf*ygty*sy*ytcqssfkgrrrrk*iinftsfc**kkrdsys
yr**kw*kl*fqn*k*hy*cfkl*ritisqr*wwfndl*srlpkysscniihyiy*w**r
nfkiqrlsn*risrkiklfrgclsfnqw*ltk*itirwle**nydsyfss*kfgwsyeni
*i*cssn---

---qfyvvhwkfysffmqimn*ivqplq*difhhqvpihtqvllvplvhymvhlmvvpmr
pywic*fisvpkrifhnsfqm*rakrrn*wvsviestktmiqeprsfve*lmkfskv*vk
nh*lk*plswrnkpwkmntl*aenfiqmlistlvssikqwvsqlicsqfyslfhvplvg*
lig*ni*kiqklksivhvkftkvkv*klcpnrw*tts*ssfsg*lfvcyh*k

Frame C:
nfiylnt*fifiil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETVTV
TDNRNGKSYDFKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGDKG
ILRYRGYPIEELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENLVGLMKTF
RYDAHP---

---sfmscignfihsscrs*iklfnrcnetyfiiryrsihkccwcrwciiwsiswwcq*g
rigyvnsyrfqreystihfrckeqkeeingfrs*nlqkl*sksqdhs*ssl*sfrkfr*r
tid*ssh*vgetslgr*ilckqktlskc*fllwshl*sngfpn*yvpsfihystcrwlvs
slgrtfrrsrn*nlsstsslqr*rfrnyvpidgrppakvrsqdsyssattkr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ723 (AFJ723Q) /CSM/AF/AFJ7-A/AFJ723Q.Seq.d/                       2210   0.0
AFN717 (AFN717Q) /CSM/AF/AFN7-A/AFN717Q.Seq.d/                       1352   0.0
AFM803 (AFM803Q) /CSM/AF/AFM8-A/AFM803Q.Seq.d/                       1338   0.0
AFJ471 (AFJ471Q) /CSM/AF/AFJ4-C/AFJ471Q.Seq.d/                       1336   0.0
AFK171 (AFK171Q) /CSM/AF/AFK1-C/AFK171Q.Seq.d/                       1326   0.0
AFJ440 (AFJ440Q) /CSM/AF/AFJ4-B/AFJ440Q.Seq.d/                       1314   0.0
AFI655 (AFI655Q) /CSM/AF/AFI6-C/AFI655Q.Seq.d/                       1308   0.0
SSF821 (SSF821Q) /CSM/SS/SSF8-A/SSF821Q.Seq.d/                       1291   0.0
SSK530 (SSK530Q) /CSM/SS/SSK5-B/SSK530Q.Seq.d/                       1271   0.0
SFE393 (SFE393Q) /CSM/SF/SFE3-D/SFE393Q.Seq.d/                       1203   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AY124377|AY124377.1 Dictyostelium discoideum citrate synthase (cshA) mRNA, complete cds.  1195  0.0  4
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    80  2e-57  8
AF310892|AF310892.1 Dictyostelium discoideum citrate synthase (gltA) gene, partial cds; RacF2 (racF2) gene, complete cds; and unknown gene.    76  9e-19  2
Z70012|Z70012.1 Bartonella sp. gltA gene (strain N40).            52  2e-12  3
BU044708|BU044708.1 PP_LEa0020E04f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0020E04f, mRNA sequence.    62  2e-10  2
BU040616|BU040616.1 PP_LEa0006L02f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0006L02f, mRNA sequence.    62  3e-10  2
AI895330|AI895330.1 EST264773 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC7M24, mRNA sequence.    46  1e-09  3
Z70011|Z70011.1 Bartonella sp. gltA gene (strain R-phy2).         52  2e-09  3
Z70022|Z70022.1 Bartonella sp. gltA gene (strain C1phy).          52  2e-09  3
BE437000|BE437000.1 EST408118 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG35K15, mRNA sequence.    46  2e-09  3
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB109907_1(AB109907|pid:none) Tetrahymena thermophila tgCS mRNA ...   307   4e-82
AK120755_1(AK120755|pid:none) Oryza sativa Japonica Group cDNA c...   306   1e-81
AB334779_1(AB334779|pid:none) Glycine max mRNA for peroxisomal c...   303   6e-81
(Q9SJH7) RecName: Full=Citrate synthase 3, peroxisomal;         ...   298   2e-79
CP000607_394(CP000607|pid:none) Chlorobium phaeovibrioides DSM 2...   297   4e-79
(P49299) RecName: Full=Citrate synthase, glyoxysomal;          E...   297   4e-79
CP000096_332(CP000096|pid:none) Pelodictyon luteolum DSM 273, co...   297   6e-79
AB167269_1(AB167269|pid:none) Chlorobium limicola cit gene for c...   294   4e-78
CP001099_363(CP001099|pid:none) Chlorobaculum parvum NCIB 8327, ...   294   4e-78
CP001110_2246(CP001110|pid:none) Pelodictyon phaeoclathratiforme...   294   5e-78
protein update 2009. 4.24
PSORT 

 psg: 0.76  gvh: 0.30  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ723 is nuc
5' end seq. ID AFJ723F
5' end seq. 
>AFJ723F.Seq
ATAATTTTATATATTTGAATACATAATTTATATTTATTATTTTATAAATATATATTTTTA
ATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAGAAGAAG
AAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAGTTACAG
TTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTATTAATG
CTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTTATGATC
CAGGCTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTGATAAAG
GAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACTTTTTAG
AGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATGGTTGGA
GTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGAAAACAT
TTAGATATGATGCTCATCCAAT----------
Length of 5' end seq. 562
3' end seq. ID AFJ723Z
3' end seq. 
>AFJ723Z.Seq
----------CCAGTTTTATGTCGTGCATTGGAAATTTTATTCATTCTTCATGCAGATCA
TGAATTAAATTGTTCAACCGCTGCAATGAGACATATTTCATCATCAGGTACCGATCCATA
CACAAGTGTTGCTGGTGCCGCTGGTGCATTATATGGTCCATCTCATGGTGGTGCCAATGA
GGCCGTATTGGATATGTTAATTCATATCGGTTCCAAAGAGAATATTCCACAATTCATTTC
AGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGAATCTACAAAAACTA
TGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTCGAAAGTTTAGGTAA
AGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTGGAAGATGAATACTT
TGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACTCTGGTCTCATCTATAAAGCAAT
GGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTCCACGTGCCGTTGGTTGGTT
AGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAATCTATCGTCCACGTCAAGT
TTACAAAGGTGAAGGTTTAGAAACTATGTCCCAATCGATGGTAGACCACCAGCTAAAGTT
CGTTCTCAGGATAGTTATTCGTCTGCTACCACTAAAAGA
Length of 3' end seq. 689
Connected seq. ID AFJ723P
Connected seq. 
>AFJ723P.Seq
ATAATTTTATATATTTGAATACATAATTTATATTTATTATTTTATAAATATATATTTTTA
ATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAGAAGAAG
AAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAGTTACAG
TTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTATTAATG
CTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTTATGATC
CAGGCTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTGATAAAG
GAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACTTTTTAG
AGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATGGTTGGA
GTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGAAAACAT
TTAGATATGATGCTCATCCAAT----------CCAGTTTTATGTCGTGCATTGGAAATTT
TATTCATTCTTCATGCAGATCATGAATTAAATTGTTCAACCGCTGCAATGAGACATATTT
CATCATCAGGTACCGATCCATACACAAGTGTTGCTGGTGCCGCTGGTGCATTATATGGTC
CATCTCATGGTGGTGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTCCAAAG
AGAATATTCCACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCG
GTCATAGAATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATG
AAGTTTTCGAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAAC
AAGCCTTGGAAGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACT
CTGGTCTCATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTA
TTCCACGTGCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTA
AAATCTATCGTCCACGTCAAGTTTACAAAGGTGAAGGTTTAGAAACTATGTCCCAATCGA
TGGTAGACCACCAGCTAAAGTTCGTTCTCAGGATAGTTATTCGTCTGCTACCACTAAAAG
A
Length of connected seq. 1251
Full length Seq ID -
Full length Seq. -
Length of full length seq. -