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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK536
Library SH
(Link to library)
Clone ID SHK536
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13024-1|Contig-U16344-1
Original site URL
Representative seq. ID SHK536P
(Link to Original site)
Representative DNA sequence 
>SHK536 (SHK536Q) /CSM/SH/SHK5-B/SHK536Q.Seq.d/
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAAXXXX
XXXXXXTGGTTCAATTGAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGT
TGGTGCTCTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACAT
TGTTGGTAACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAAT
GTCAATCACCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGG
TAAGACCACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCC
AATGATCGTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGA
AGCCAATCAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGT
TAAAGGTAATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACA
AGCTGCCGGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGT
TTGGTCAATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGA
TTCAACTCCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCT
TTATTCTAACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAA
TTTTAAAACAATAAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
iqstnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---GSIESKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKMAATATVYANIDAL
MSITTSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVAS
EANQAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASK
VWSIVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlf
nfktiki
Translated Amino Acid sequence (All Frames) 
Frame A:
iqstnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---wfn*iksy*fhswyhsrcwcs*mwyl*lcq*drc*pqhcw*r*ngrncnclckh*cf
nvnhhlnpnlnlsiinqdcqr*dhhsiiikliiihl*tirsndrlfryc*ccw*pqcrcl
rsqss*nclwyciqihlspsc*r*clls*lshlkhccccstscrccccccpkscygccl*
slvnckftm*l*mwieeiin*fnssspmtin*nntthsyfslf*pmylsinlltf*thpf
qf*nnkn

Frame B:
fnqqtknnnlkkq*fivk*kqlknh*lffspphykfqyffln*fpaflffytittnpisl
lptlyhynliy*iiiicciilvyic*fniy*qvtnkkk---

---GSIESKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKMAATATVYANIDAL
MSITTSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVAS
EANQAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASK
VWSIVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlf
nfktiki

Frame C:
sinkqktti*knnnl**sknn*ritncffllltinsniff*infqlfcfftqsqptlyhy
yqpytitilfik*llfvvlf*cifvdlifisk*qtkkk---

---vqlnqkllislmvpfkvlvllnvvlvtlpirslltstllvtlkwpqlqlsmqtlml*
cqsppqpkpqslnhqprlstvrpplnhhqahhhpslnnsfq*sfvqvllmllvtsmslpq
kpiklklplvlysnssqpqllkvmspqlavspqalllllnklpvlllllpkkllwllplk
fgql*ihhvtvnvd*rdykliqlllsndnklk*ynsflfffiltnvfvn*linflntpfs
ilkq*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE342 (SLE342Q) /CSM/SL/SLE3-B/SLE342Q.Seq.d/                        900   0.0
SLA101 (SLA101Q) /CSM/SL/SLA1-A/SLA101Q.Seq.d/                        900   0.0
SHL563 (SHL563Q) /CSM/SH/SHL5-C/SHL563Q.Seq.d/                        900   0.0
SHK865 (SHK865Q) /CSM/SH/SHK8-C/SHK865Q.Seq.d/                        900   0.0
SHK762 (SHK762Q) /CSM/SH/SHK7-C/SHK762Q.Seq.d/                        900   0.0
SHK536 (SHK536Q) /CSM/SH/SHK5-B/SHK536Q.Seq.d/                        900   0.0
SHG713 (SHG713Q) /CSM/SH/SHG7-A/SHG713Q.Seq.d/                        900   0.0
SHG514 (SHG514Q) /CSM/SH/SHG5-A/SHG514Q.Seq.d/                        900   0.0
SHG338 (SHG338Q) /CSM/SH/SHG3-B/SHG338Q.Seq.d/                        900   0.0
SHG152 (SHG152Q) /CSM/SH/SHG1-C/SHG152Q.Seq.d/                        900   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83087|U83087.1 Dictyostelium discoideum rsc12 mRNA, partial cds.   900   0.0    2
AX344185|AX344185.1 Sequence 32 from Patent WO0200926.             40   0.081  4
AX251781|AX251781.1 Sequence 42 from Patent WO0168911.             40   0.081  4
BD460245|BD460245.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.081  4
BD452167|BD452167.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.081  4
AJ297856|AJ297856.1 Lucilia sericata partial bcd gene for bicoid protein.    50   0.11   1
AL845362|AL845362.4 Zebrafish DNA sequence from clone CH211-142H2 in linkage group 20.    38   0.32   3
AC162431|AC162431.1 Papio hamadryas clone RP41-121N17, WORKING DRAFT SEQUENCE.    48   0.42   1
AC156169|AC156169.2 Bos taurus clone CH240-50N13, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    48   0.42   1
AC127176|AC127176.3 Rattus norvegicus clone CH230-267P11, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.42   1
dna update 2006. 6.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83087_1(U83087|pid:none) Dictyostelium discoideum rsc12 mRNA, p...   269   1e-70
AY530539_1(AY530539|pid:none) Mimulus ringens deficiens (DEF) mR...    34   9.2
protein update 2009. 1.17
PSORT 

 psg: 0.69  gvh: 0.45  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHK536 is cyt
5' end seq. ID SHK536F
5' end seq. 
>SHK536F.Seq
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAANNNN
NNNNNN
Length of 5' end seq. 306
3' end seq. ID SHK536Z
3' end seq. 
>SHK536Z.Seq
NNNNNNNNNNTGGTTCAATTGAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAG
GTGTTGGTGCTCTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCA
ACATTGTTGGTAACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTT
TAATGTCAATCACCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCA
ACGGTAAGACCACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCG
TTCCAATGATCGTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCT
CAGAAGCCAATCAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAG
TTGTTAAAGGTAATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTC
AACAAGCTGCCGGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTA
AAGTTTGGTCAATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAA
TTGATTCAACTCCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTT
TTCTTTATTCTAACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTT
TCAATTTTAAAACAATAAAAATT
Length of 3' end seq. 743
Connected seq. ID SHK536P
Connected seq. 
>SHK536P.Seq
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAA----
------TGGTTCAATTGAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGT
TGGTGCTCTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACAT
TGTTGGTAACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAAT
GTCAATCACCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGG
TAAGACCACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCC
AATGATCGTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGA
AGCCAATCAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGT
TAAAGGTAATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACA
AGCTGCCGGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGT
TTGGTCAATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGA
TTCAACTCCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCT
TTATTCTAACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAA
TTTTAAAACAATAAAAATT
Length of connected seq. 1029
Full length Seq ID -
Full length Seq. -
Length of full length seq. -