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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK515
Library SH
(Link to library)
Clone ID SHK515
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13024-1|Contig-U16344-1
Original site URL
Representative seq. ID SHK515P
(Link to Original site)
Representative DNA sequence 
>SHK515 (SHK515Q) /CSM/SH/SHK5-A/SHK515Q.Seq.d/
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAAXXXX
XXXXXXAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTA
AATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACG
TTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCA
CCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCA
CTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTT
GTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAG
CTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATG
TCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTG
CTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTG
TAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTC
CTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACC
AATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAA
TAAAAATTTACATAT
sequence update 2002.10.25
Translated Amino Acid sequence 
iqstnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---SKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKMAATATVYANIDALMSIT
TSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVASEANQ
AKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASKVWSI
VNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlfnfkt
ikiyi
Translated Amino Acid sequence (All Frames) 
Frame A:
iqstnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---nqkllislmvpfkvlvllnvvlvtlpirslltstllvtlkwpqlqlsmqtlml*cqs
ppqpkpqslnhqprlstvrpplnhhqahhhpslnnsfq*sfvqvllmllvtsmslpqkpi
klklplvlysnssqpqllkvmspqlavspqalllllnklpvlllllpkkllwllplkfgq
l*ihhvtvnvd*rdykliqlllsndnklk*ynsflfffiltnvfvn*linflntpfsilk
q*kfty

Frame B:
fnqqtknnnlkkq*fivk*kqlknh*lffspphykfqyffln*fpaflffytittnpisl
lptlyhynliy*iiiicciilvyic*fniy*qvtnkkk---

---iksy*fhswyhsrcwcs*mwyl*lcq*drc*pqhcw*r*ngrncnclckh*cfnvnh
hlnpnlnlsiinqdcqr*dhhsiiikliiihl*tirsndrlfryc*ccw*pqcrclrsqs
s*nclwyciqihlspsc*r*clls*lshlkhccccstscrccccccpkscygccl*slvn
ckftm*l*mwieeiin*fnssspmtin*nntthsyfslf*pmylsinlltf*thpfqf*n
nknlh

Frame C:
sinkqktti*knnnl**sknn*ritncffllltinsniff*infqlfcfftqsqptlyhy
yqpytitilfik*llfvvlf*cifvdlifisk*qtkkk---

---SKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKMAATATVYANIDALMSIT
TSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVASEANQ
AKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASKVWSI
VNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlfnfkt
ikiyi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE342 (SLE342Q) /CSM/SL/SLE3-B/SLE342Q.Seq.d/                        878   0.0
SLA101 (SLA101Q) /CSM/SL/SLA1-A/SLA101Q.Seq.d/                        878   0.0
SHL563 (SHL563Q) /CSM/SH/SHL5-C/SHL563Q.Seq.d/                        878   0.0
SHL307 (SHL307Q) /CSM/SH/SHL3-A/SHL307Q.Seq.d/                        878   0.0
SHL203 (SHL203Q) /CSM/SH/SHL2-A/SHL203Q.Seq.d/                        878   0.0
SHK865 (SHK865Q) /CSM/SH/SHK8-C/SHK865Q.Seq.d/                        878   0.0
SHK762 (SHK762Q) /CSM/SH/SHK7-C/SHK762Q.Seq.d/                        878   0.0
SHK650 (SHK650Q) /CSM/SH/SHK6-C/SHK650Q.Seq.d/                        878   0.0
SHK536 (SHK536Q) /CSM/SH/SHK5-B/SHK536Q.Seq.d/                        878   0.0
SHK515 (SHK515Q) /CSM/SH/SHK5-A/SHK515Q.Seq.d/                        878   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83087|U83087.1 Dictyostelium discoideum rsc12 mRNA, partial cds.   878   0.0    2
AX344185|AX344185.1 Sequence 32 from Patent WO0200926.             40   0.080  4
AX251781|AX251781.1 Sequence 42 from Patent WO0168911.             40   0.080  4
BD460245|BD460245.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.080  4
BD452167|BD452167.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.080  4
AJ297856|AJ297856.1 Lucilia sericata partial bcd gene for bicoid protein.    50   0.11   1
AC022143|AC022143.7 Homo sapiens chromosome 19 clone CTD-2378H7, complete sequence.    48   0.18   2
AL845362|AL845362.4 Zebrafish DNA sequence from clone CH211-142H2 in linkage group 20.    38   0.32   3
AC170957|AC170957.2 Bos taurus clone CH240-254I20, WORKING DRAFT SEQUENCE, 13 unordered pieces.    48   0.42   1
AC162431|AC162431.1 Papio hamadryas clone RP41-121N17, WORKING DRAFT SEQUENCE.    48   0.42   1
dna update 2006. 6.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83087_1(U83087|pid:none) Dictyostelium discoideum rsc12 mRNA, p...   262   2e-68
AY530539_1(AY530539|pid:none) Mimulus ringens deficiens (DEF) mR...    34   9.2
protein update 2009. 1.17
PSORT 

 psg: 0.65  gvh: 0.45  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHK515 is cyt
5' end seq. ID SHK515F
5' end seq. 
>SHK515F.Seq
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAANNNN
NNNNNN
Length of 5' end seq. 306
3' end seq. ID SHK515Z
3' end seq. 
>SHK515Z.Seq
NNNNNNNNNNAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCT
CTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGT
AACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATC
ACCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACC
ACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATC
GTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAAT
CAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGT
AATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCC
GGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCA
ATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACT
CCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCT
AACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAA
ACAATAAAAATTTACATAT
Length of 3' end seq. 739
Connected seq. ID SHK515P
Connected seq. 
>SHK515P.Seq
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAA----
------AATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTA
AATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACG
TTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCA
CCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCA
CTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTT
GTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAG
CTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATG
TCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTG
CTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTG
TAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTC
CTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACC
AATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAA
TAAAAATTTACATAT
Length of connected seq. 1025
Full length Seq ID -
Full length Seq. -
Length of full length seq. -