>AFJ691 (AFJ691Q) /CSM/AF/AFJ6-D/AFJ691Q.Seq.d/
CACTGTTGGCCTACTGGATTTTTCATATTTATATATAATATTACACCCCATAACTAATAA
AATGTCATTCAATTCAAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTT
AGAATCAAATCCAACTTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACC
ATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGA
TTTATTAGCACCAGTTATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAAT
TGGAGATGTTGTTATGGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAG
AGTTGCCACATTATTAGCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACA
ATGTTCATCAGGTTTACAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTA
TGACATTGGTTTAGCAGGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGG
TGGTTTCAACGAGGTTGCXXXXXXXXXXTCTGAAAATGTCGCAGAGCGTTTTGGTGTCAC
CAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAA
AGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAAC
CATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAA
ATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAG
TGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAA
GGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTAT
TGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATAT
CGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTT
GGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATT
AGGTGCTACTGGTGCCCGTCAAAGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGT
AAATATGGTGTATCTCTAT

TVGLLDFSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAP
FRTAICKAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVR
VATLLAGLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDG
GFNEV---

---SENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKD
EGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFV
AFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNK
VNPNGSGIALGHPLGATGARQRLPFQENYIVVKVNMVYLY

Frame A:
hcwptgffifiynitphn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncct
isysnl*s*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*
schiisrftkgstmlysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefksngmgw
wfqrgc---

---SENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKD
EGIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFV
AFAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNK
VNPNGSGIALGHPLGATGARQRLPFQENYIVVKVNMVYLY

Frame B:
TVGLLDFSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAP
FRTAICKAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVR
VATLLAGLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDG
GFNEV---

---lkmsqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssikt
kvsvrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*
llllsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tk
liqmalvsl*vih*vllvpvkgyhfkritss*r*iwcis

Frame C:
llaywifhiyi*yytp*likchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhh
fvqqfvklkeehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpkle
lphy*qvyqrkyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*iqwhgmv
vstrl---

---*kcrrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rr
ryp*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrs
fcccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs
*skwlwyrfrssircywcpskatisrelhrrkgkygvsl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ691 (AFJ691Q) /CSM/AF/AFJ6-D/AFJ691Q.Seq.d/                       2357   0.0
SFL240 (SFL240Q) /CSM/SF/SFL2-B/SFL240Q.Seq.d/                       1273   0.0
VFL606 (VFL606Q) /CSM/VF/VFL6-A/VFL606Q.Seq.d/                       1263   0.0
VFJ317 (VFJ317Q) /CSM/VF/VFJ3-A/VFJ317Q.Seq.d/                       1263   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1263   0.0
SLG762 (SLG762Q) /CSM/SL/SLG7-C/SLG762Q.Seq.d/                       1263   0.0
SFK756 (SFK756Q) /CSM/SF/SFK7-C/SFK756Q.Seq.d/                       1263   0.0
SFH501 (SFH501Q) /CSM/SF/SFH5-A/SFH501Q.Seq.d/                       1263   0.0
SFG758 (SFG758Q) /CSM/SF/SFG7-C/SFG758Q.Seq.d/                       1263   0.0
SFG482 (SFG482Q) /CSM/SF/SFG4-D/SFG482Q.Seq.d/                       1263   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1180  0.0  4
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60  1e-13  5
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60  6e-12  4
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44  2e-10  4
CF213585|CF213585.1 CGF1000794_F07 Vitis vinifera cv. cabernet sauvignon Stem - CAST Vitis vinifera cDNA clone CAST0006_IF_F07 5', mRNA sequence.    60  3e-10  3
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68  5e-10  3
CD007738|CD007738.1 VVB078G11_334604 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB078G11 5, mRNA sequence.    60  1e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68  1e-08  2
CD716621|CD716621.1 VVB129E11_405541 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB129E11 5, mRNA sequence.    60  1e-08  2
CD008871|CD008871.1 VVB097G11_339261 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB097G11 5, mRNA sequence.    60  1e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   675   0.0
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   388   e-106
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   387   e-106
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   385   e-105
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   385   e-105
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   381   e-104
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   381   e-104
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   381   e-104
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   381   e-104
BT070433_1(BT070433|pid:none) Picea sitchensis clone WS02729_F15...   380   e-104

 psg: 0.75  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.26  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFJ691 is nuc

>AFJ691F.Seq
CACTGTTGGCCTACTGGATTTTTCATATTTATATATAATATTACACCCCATAACTAATAA
AATGTCATTCAATTCAAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTT
AGAATCAAATCCAACTTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACC
ATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGA
TTTATTAGCACCAGTTATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAAT
TGGAGATGTTGTTATGGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAG
AGTTGCCACATTATTAGCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACA
ATGTTCATCAGGTTTACAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTA
TGACATTGGTTTAGCAGGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGG
TGGTTTCAACGAGGTTGC----------

>AFJ691Z.Seq
----------TCTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGA
ATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGA
TGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGA
CGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAA
AGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTG
TTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGT
AGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAAT
TCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAA
TGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAA
AGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCG
TCAAAGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTATCTCTA
T

>AFJ691P.Seq
CACTGTTGGCCTACTGGATTTTTCATATTTATATATAATATTACACCCCATAACTAATAA
AATGTCATTCAATTCAAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTT
AGAATCAAATCCAACTTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACC
ATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGA
TTTATTAGCACCAGTTATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAAT
TGGAGATGTTGTTATGGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAG
AGTTGCCACATTATTAGCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACA
ATGTTCATCAGGTTTACAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTA
TGACATTGGTTTAGCAGGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGG
TGGTTTCAACGAGGTTGC----------TCTGAAAATGTCGCAGAGCGTTTTGGTGTCAC
CAGAAAAGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAA
AGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAAC
CATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAA
ATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAG
TGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAA
GGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTAT
TGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATAT
CGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTT
GGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATT
AGGTGCTACTGGTGCCCGTCAAAGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGT
AAATATGGTGTATCTCTAT