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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK285
Library SH
(Link to library)
Clone ID SHK285
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15685-1
Original site URL
Representative seq. ID SHK285P
(Link to Original site)
Representative DNA sequence 
>SHK285 (SHK285Q) /CSM/SH/SHK2-D/SHK285Q.Seq.d/
ACTGTTGGCCTACTGGNATTTTCAAAAAACTATTTAAAAAAAAATAGGAGNAATATAGTT
GTATATATAACACACAAAAAAATTCATATCACATTATTGTTTTTCATTAATTTATAAACA
ATAATATATATATAATAAACACTCTATCATTTAATTTTAATAAAAAAAAAAXXXXXXXXX
XGTTGATCCACCATTCATTCTTCCAACCATTATCGCTATGTTCTTGTCACCAGGTGGTAC
ACCAGATGTTGTCTTCTTCTCTGGTCAAGGTGCTGTTCAAACCGCTCTCTTATTCTTGGC
TCTCATTTCCATTCCAGTTATGTTGGTCATTAAACCATTGTTCATGAAGAGATTCCACTT
CCAAGAAGTCGAAAGAAAGAAATTAGGTCACCACGAAGAGGAACATGATGATGAAGCTCT
CTACACTGGTCATCACGGTGAAGAATTTGAAATGGGTGAAGTCTTTGTTCATCAAGTCAT
TCATACCATCGAGTTTGTTTTGGGTGCTGTTTCAAATACTGCCTCTTATCTTCGTTTATG
GGCTCTTTCATTGGCTCACTCTGAGTTATCTTCTGTATTTTGGGAACGTATTCTCATTGG
TCAAGTCGAACGTGGTAATCCATTCTTGGCTTTCGTTGGTTTTGGTGCTTGGTTAGGTGC
CTCTGTTGCTGTACCTTTTANTTATGGAATCCCTTTCTGCTTTCTTGCACGCTCTTAGAC
TTCATTGGGTAGAATTCCAAAATAAATTCTATATTGGTGATGGTGTTAGATTCATTCCAT
ACAGTNCCACTCGTATTCT
sequence update 2002.10.25
Translated Amino Acid sequence 
cwptgifkklfkkk*exysciyntqknsyhiivfh*FINNNIYIINTLSFNFNKKK---

---VDPPFILPTIIAMFLSPGGTPDVVFFSGQGAVQTALLFLALISIPVMLVIKPLFMKR
FHFQEVERKKLGHHEEEHDDEALYTGHHGEEFEMGEVFVHQVIHTIEFVLGAVSNTASYL
RLWALSLAHSELSSVFWERILIGQVERGNPFLAFVGFGAWLGASVAVPFXYGIPFCFLAR
S*tslgripk*ilyw*wc*ihsiqxhsys
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxfsknylkknrxnivvyithkkihitllffinl*tiiyi**tlyhlilikkk---

---VDPPFILPTIIAMFLSPGGTPDVVFFSGQGAVQTALLFLALISIPVMLVIKPLFMKR
FHFQEVERKKLGHHEEEHDDEALYTGHHGEEFEMGEVFVHQVIHTIEFVLGAVSNTASYL
RLWALSLAHSELSSVFWERILIGQVERGNPFLAFVGFGAWLGASVAVPFXYGIPFCFLAR
S*tslgripk*ilyw*wc*ihsiqxhsys

Frame B:
llaywxfqkti*kkigxi*lyi*htkkfishycfsliykq*yiynkhsii*f**kk---

---lihhsffqplslcschqvvhqmlssslvkvlfkplsyswlsfpfqlcwslnhcs*rd
stskkskern*vttkrnmmmklstlvitvknlkwvkslfiksfipsslfwvlfqilplif
vyglfhwltlsyllyfgnvfslvksnvvihswlslvlvlg*vplllyllxmeslsaflha
lrlhwvefqnkfyigdgvrfipysxtri

Frame C:
cwptgifkklfkkk*exysciyntqknsyhiivfh*FINNNIYIINTLSFNFNKKK---

---*stihssnhyryvlvtrwytrcclllwsrccsnrslilgshfhssyvgh*tivheei
plprsrkkeirsprrgt***sslhwssr*ri*ng*slcssshsyhrvcfgccfkycllss
fmgsfigsl*vifcilgtyshwssrtw*silgfrwfwclvrclccctfxlwnpfllsctl
ldfig*nskinsilvmvldsfhtvplvf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK285 (SHK285Q) /CSM/SH/SHK2-D/SHK285Q.Seq.d/                       1213   0.0
VHN293 (VHN293Q) /CSM/VH/VHN2-D/VHN293Q.Seq.d/                       1197   0.0
CHR818 (CHR818Q) /CSM/CH/CHR8-A/CHR818Q.Seq.d/                       1197   0.0
CHR349 (CHR349Q) /CSM/CH/CHR3-C/CHR349Q.Seq.d/                       1197   0.0
CHH295 (CHH295Q) /CSM/CH/CHH2-D/CHH295Q.Seq.d/                       1197   0.0
CHF322 (CHF322Q) /CSM/CH/CHF3-A/CHF322Q.Seq.d/                       1197   0.0
CHL154 (CHL154Q) /CSM/CH/CHL1-C/CHL154Q.Seq.d/                       1195   0.0
SHG617 (SHG617Q) /CSM/SH/SHG6-A/SHG617Q.Seq.d/                       1191   0.0
SHG537 (SHG537Q) /CSM/SH/SHG5-B/SHG537Q.Seq.d/                       1191   0.0
CHO859 (CHO859Q) /CSM/CH/CHO8-C/CHO859Q.Seq.d/                       1191   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U38803|U38803.1 Dictyostelium discoideum vacuolar proton ATPase 100-kDa subunit (vatM) mRNA, complete cds.   977   0.0    2
BF272276|BF272276.2 GA__Eb0014G21f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Eb0014G21f, mRNA sequence.    72   2e-13  2
DY983049|DY983049.1 CLSS5348.b1_H18.ab1 CLS(LMS) lettuce sativa Lactuca sativa cDNA clone CLSS5348, mRNA sequence.    80   9e-11  1
BU012351|BU012351.1 QGJ1L02.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGJ1L02, mRNA sequence.    80   9e-11  1
BQ853466|BQ853466.1 QGB20J11.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGB20J11, mRNA sequence.    80   9e-11  1
AC137668|AC137668.18 Medicago truncatula clone mth2-5f8, complete sequence.    52   4e-09  4
CV164410|CV164410.1 rsmsxl_003361.y1.scf cDNA Library of Salvia miltiorrhiza Salvia miltiorrhiza cDNA 5', mRNA sequence.    54   6e-09  3
CV172465|CV172465.1 rsmsxlre_008717.z1.scf cDNA Library of Salvia miltiorrhiza Salvia miltiorrhiza cDNA 5', mRNA sequence.    54   1e-08  3
DT466948|DT466948.1 GH_CHX19K13.f GH_CHX Gossypium hirsutum cDNA clone GH_CHX19K13 5', mRNA sequence.    72   2e-08  1
DT457612|DT457612.1 GH_ON34M24.r GH_ON Gossypium hirsutum cDNA clone GH_ON34M24 3', mRNA sequence.    72   2e-08  1
dna update 2006. 6. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54E04) RecName: Full=Vacuolar proton translocating ATPase 100 ...   332   e-104
U38803_1(U38803|pid:none) Dictyostelium discoideum vacuolar prot...   332   e-104
AC093568_14(AC093568|pid:none) Oryza sativa chromosome 10 clone ...   142   7e-44
AK101151_1(AK101151|pid:none) Oryza sativa Japonica Group cDNA c...   139   1e-42
AL035679_18(AL035679|pid:none) Arabidopsis thaliana DNA chromoso...   138   8e-42
AK227321_1(AK227321|pid:none) Arabidopsis thaliana mRNA for hypo...   138   8e-42
CP000588_396(CP000588|pid:none) Ostreococcus lucimarinus CCE9901...   143   1e-41
AK230210_1(AK230210|pid:none) Arabidopsis thaliana mRNA for hypo...   137   1e-41
CR954208_391(CR954208|pid:none) Ostreococcus tauri strain OTTH05...   135   5e-41
AK221976_1(AK221976|pid:none) Arabidopsis thaliana mRNA for hypo...   135   7e-41
protein update 2009. 5.30
PSORT 

 psg: 0.85  gvh: 0.49  alm: 0.18  top: 0.77  tms: 0.20  mit: 0.30  mip: 0.13
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	 16.0 %: vacuolar
	 12.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for SHK285 is end
5' end seq. ID SHK285F
5' end seq. 
>SHK285F.Seq
ACTGTTGGCCTACTGGNATTTTCAAAAAACTATTTAAAAAAAAATAGGAGNAATATAGTT
GTATATATAACACACAAAAAAATTCATATCACATTATTGTTTTTCATTAATTTATAAACA
ATAATATATATATAATAAACACTCTATCATTTAATTTTAATAAAAAAAAAANNNNNNNNN
N
Length of 5' end seq. 181
3' end seq. ID SHK285Z
3' end seq. 
>SHK285Z.Seq
NNNNNNNNNNGTTGATCCACCATTCATTCTTCCAACCATTATCGCTATGTTCTTGTCACC
AGGTGGTACACCAGATGTTGTCTTCTTCTCTGGTCAAGGTGCTGTTCAAACCGCTCTCTT
ATTCTTGGCTCTCATTTCCATTCCAGTTATGTTGGTCATTAAACCATTGTTCATGAAGAG
ATTCCACTTCCAAGAAGTCGAAAGAAAGAAATTAGGTCACCACGAAGAGGAACATGATGA
TGAAGCTCTCTACACTGGTCATCACGGTGAAGAATTTGAAATGGGTGAAGTCTTTGTTCA
TCAAGTCATTCATACCATCGAGTTTGTTTTGGGTGCTGTTTCAAATACTGCCTCTTATCT
TCGTTTATGGGCTCTTTCATTGGCTCACTCTGAGTTATCTTCTGTATTTTGGGAACGTAT
TCTCATTGGTCAAGTCGAACGTGGTAATCCATTCTTGGCTTTCGTTGGTTTTGGTGCTTG
GTTAGGTGCCTCTGTTGCTGTACCTTTTANTTATGGAATCCCTTTCTGCTTTCTTGCACG
CTCTTAGACTTCATTGGGTAGAATTCCAAAATAAATTCTATATTGGTGATGGTGTTAGAT
TCATTCCATACAGTNCCACTCGTATTCT
Length of 3' end seq. 628
Connected seq. ID SHK285P
Connected seq. 
>SHK285P.Seq
ACTGTTGGCCTACTGGNATTTTCAAAAAACTATTTAAAAAAAAATAGGAGNAATATAGTT
GTATATATAACACACAAAAAAATTCATATCACATTATTGTTTTTCATTAATTTATAAACA
ATAATATATATATAATAAACACTCTATCATTTAATTTTAATAAAAAAAAAA---------
-GTTGATCCACCATTCATTCTTCCAACCATTATCGCTATGTTCTTGTCACCAGGTGGTAC
ACCAGATGTTGTCTTCTTCTCTGGTCAAGGTGCTGTTCAAACCGCTCTCTTATTCTTGGC
TCTCATTTCCATTCCAGTTATGTTGGTCATTAAACCATTGTTCATGAAGAGATTCCACTT
CCAAGAAGTCGAAAGAAAGAAATTAGGTCACCACGAAGAGGAACATGATGATGAAGCTCT
CTACACTGGTCATCACGGTGAAGAATTTGAAATGGGTGAAGTCTTTGTTCATCAAGTCAT
TCATACCATCGAGTTTGTTTTGGGTGCTGTTTCAAATACTGCCTCTTATCTTCGTTTATG
GGCTCTTTCATTGGCTCACTCTGAGTTATCTTCTGTATTTTGGGAACGTATTCTCATTGG
TCAAGTCGAACGTGGTAATCCATTCTTGGCTTTCGTTGGTTTTGGTGCTTGGTTAGGTGC
CTCTGTTGCTGTACCTTTTANTTATGGAATCCCTTTCTGCTTTCTTGCACGCTCTTAGAC
TTCATTGGGTAGAATTCCAAAATAAATTCTATATTGGTGATGGTGTTAGATTCATTCCAT
ACAGTNCCACTCGTATTCT
Length of connected seq. 789
Full length Seq ID -
Full length Seq. -
Length of full length seq. -