>SHK254 (SHK254Q) /CSM/SH/SHK2-C/SHK254Q.Seq.d/
ACTGTTGGCCTACTGGNATTGTATATAATATTTTTAAATATATATATTAATATATATTAA
TATATTAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATT
TTAAATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAA
GGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTT
GGAAATGTTGAAATTTCTTTAGGAAATGCAACTGACTATAAGGGAAATAAATATCCAATA
CGTTATTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTAT
GAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCT
TGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGAT
CTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCA
TCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACT
CAACCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCAXXXXXX
XXXXAGTGGAGGTAAATGTGGTAGTCTTGTTGGTGCCGNAGTTGATCAAAAATCACTCAC
TGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCA
TGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGG
TTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAA
ACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAAC
CTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACA
ATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGA
ACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAG
TCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTA
TTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGC
TGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTTA
TGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATTATTTTAATGGTTGTTCATTGGTA
TTATTCCAAAAAAATAAAATAATAAAAAAATATAAATA

tvgllxlyiiflniyiniy*YININKMKILYSLLLISSIILNTVLNISSQVYDQRILALK
GKCYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRYSSADGTFIYDGLTQRTTY
EFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVS
SGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPP---

---SGGKCGSLVGAXVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVT
TGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSL
PQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSS
NYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIILMVVH
WYYSKKIK**knin

Frame A:
tvgllxlyiiflniyiniy*YININKMKILYSLLLISSIILNTVLNISSQVYDQRILALK
GKCYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRYSSADGTFIYDGLTQRTTY
EFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVS
SGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPP---

---SGGKCGSLVGAXVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVT
TGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSL
PQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSS
NYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIILMVVH
WYYSKKIK**knin

Frame B:
llaywxci*yf*iyiliyinililik*kyyihyy*yqv*f*ily*ifhhkyminvfwh*k
envi*iqikmhcmmkvnqdlemlkfl*emqltireiniqyvihllmvhlymmd*lnvqlm
nlhlpiqkiitlkpmkrhwlvhqvyhvfvqrfqliiwqqif*lqiplpitfh*lnklvfh
qahlylqlelvliwvmlsklnhqlnhllnhllnhqhl---

---vevnvvvllvpxliknhslvldmvqvmvisliiikqvfmtipmllvqlkvvvilllq
lvsitmkhlkvqfvgimqplvnn*kvtlyi*plqmvvphlqplvnkmvlvillqcmillf
hnivlvefgli*mvmvfkmlvnlvfqesqys*smvnnqlqqvklypiqmvisnlklisvq
iiiv*fllhssklvlplq*emliqliqiqivmlsyqllimfmsllllllmlgllf*wlfi
giipkk*nnkki*i

Frame C:
cwptgivynifkyiy*yiliy*y**neniifiiinikynfkycikyfitsi*styfgikr
kmlfrfk*kciv**r*triwkc*nffrkcn*l*gk*isntlfic*wyiyi*winstynl*
iyiyqskrlll*nl*kdiglstrctmyssngfs**fgsrsfsckylcl*hsid*tswcfi
rliytys*n*c*fglc*ansttnsttysttysttnts---

---wr*mw*scwcrs*skithws*iwsr*w*fl**s*nrss*lyqcfwcn*rws*yycyn
wfrll*si*rfnslv*cnlw*ttkrllfiynlfkwsypisnlw*tkwsw*yyfsv*ffss
tiscw*sla*ykw*wysrcw*twyfrnhsilnlwstinsnksncirfkwlfqi*s*fqfk
lllcnsccilpswffhynekc*snsskyk**cflinc**clchffyff*cwgyyfngcsl
vlfqknkiikkyk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK254 (SHK254Q) /CSM/SH/SHK2-C/SHK254Q.Seq.d/                       2432   0.0
SHL582 (SHL582Q) /CSM/SH/SHL5-D/SHL582Q.Seq.d/                       1427   0.0
SHL103 (SHL103Q) /CSM/SH/SHL1-A/SHL103Q.Seq.d/                       1427   0.0
SHD494 (SHD494Q) /CSM/SH/SHD4-D/SHD494Q.Seq.d/                       1427   0.0
SHA857 (SHA857Q) /CSM/SH/SHA8-C/SHA857Q.Seq.d/                       1427   0.0
SHA769 (SHA769Q) /CSM/SH/SHA7-C/SHA769Q.Seq.d/                       1427   0.0
SHA116 (SHA116Q) /CSM/SH/SHA1-A/SHA116Q.Seq.d/                       1427   0.0
CHM487 (CHM487Q) /CSM/CH/CHM4-D/CHM487Q.Seq.d/                       1427   0.0
CHM456 (CHM456Q) /CSM/CH/CHM4-C/CHM456Q.Seq.d/                       1427   0.0
CHG647 (CHG647Q) /CSM/CH/CHG6-B/CHG647Q.Seq.d/                       1427   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   4e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   4e-05  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   4e-05  1
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   4e-05  3
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   6e-04  3
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   6e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   6e-04  1
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   6e-04  1
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.002  3
DT775356|DT775356.1 124719991 TH1 Tribolium castaneum cDNA clone 3C7 3', mRNA sequence.    54   0.009  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    78   9e-13
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    63   2e-08
CP000910_1528(CP000910|pid:none) Renibacterium salmoninarum ATCC...    61   1e-07
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    59   4e-07
(Q7A780) RecName: Full=Serine-aspartate repeat-containing protei...    53   3e-05
AM076203_1(AM076203|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076200_1(AM076200|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076196_1(AM076196|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076198_1(AM076198|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
CP000703_575(CP000703|pid:none) Staphylococcus aureus subsp. aur...    53   3e-05

 psg: 0.95  gvh: 0.59  alm: 0.26  top: 0.40  tms: 0.07  mit: 0.31  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.56  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 12.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for SHK254 is mit

>SHK254F.Seq
ACTGTTGGCCTACTGGNATTGTATATAATATTTTTAAATATATATATTAATATATATTAA
TATATTAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATT
TTAAATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAA
GGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTT
GGAAATGTTGAAATTTCTTTAGGAAATGCAACTGACTATAAGGGAAATAAATATCCAATA
CGTTATTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTAT
GAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCT
TGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGAT
CTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCA
TCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACT
CAACCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCANNNNNN
NNNN

>SHK254Z.Seq
NNNNNNNNNNAGTGGAGGTAAATGTGGTAGTCTTGTTGGTGCCGNAGTTGATCAAAAATC
ACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGG
TCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTAC
AACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTC
TGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATC
TCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCT
TCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGC
TGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCC
AACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTC
AAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAG
AAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAA
TGTTTATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATTATTTTAATGGTTGTTCA
TTGGTATTATTCCAAAAAAATAAAATAATAAAAAAATATAAATA

>SHK254P.Seq
ACTGTTGGCCTACTGGNATTGTATATAATATTTTTAAATATATATATTAATATATATTAA
TATATTAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATT
TTAAATACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAA
GGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTT
GGAAATGTTGAAATTTCTTTAGGAAATGCAACTGACTATAAGGGAAATAAATATCCAATA
CGTTATTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTAT
GAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCT
TGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGAT
CTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCA
TCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACT
CAACCACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCA------
----AGTGGAGGTAAATGTGGTAGTCTTGTTGGTGCCGNAGTTGATCAAAAATCACTCAC
TGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCA
TGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGG
TTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAA
ACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAAC
CTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACA
ATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGA
ACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAG
TCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTA
TTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGC
TGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTTA
TGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATTATTTTAATGGTTGTTCATTGGTA
TTATTCCAAAAAAATAAAATAATAAAAAAATATAAATA