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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK247
Library SH
(Link to library)
Clone ID SHK247
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10899-1|Contig-U12190-1
Original site URL
Representative seq. ID SHK247P
(Link to Original site)
Representative DNA sequence 
>SHK247 (SHK247Q) /CSM/SH/SHK2-B/SHK247Q.Seq.d/
AAATATTCACCTCTTTCCCCACATATAAATAAAAATGTCAAATGAAGATGGTGAAATTAT
AAGCCACCAATATCAAAGNAGGCAGGGATTCTGAATATGATAGTAGAAGAGAAAGAGATT
ATAAAGATTCATATGATAATAAATCAAAAGACAGNAGAAAGAAGTAAAAGTAGAAGTAGA
AGTAGGGATAGAGATAGAAATAGATACGATAGAGATAGAGAAAGAGATTTAGAAAAAGAA
AGAGAAAGAGATAGAGACCATGACAGAAATAGAGATAGAGATAGAGACCGTGACAGAAAT
AGAGATAGAGATAGAGACCACGACAGAAATAGAGATAGAGATAGAAATAGAGACCGTGAC
CATGACCGTGATAGAGATAGGGAAAGAGAAAAAGACAGAGAAAGAGATAGAGATAGAGAT
AGATACGGTAACAAAGAAAGAGATAGGGATAGGGATAGAGAAAGAGATAGAGATAGAGAA
AGGGATAGAGAAAGAGAAAGAGAAAAAGAAAGAGAAAGAGAAAGAGAAAGAGATAGAGAT
AGATATGGTGATAGATCAACCTCAAGAXXXXXXXXXXATTTAGCAATTATGTCAAGTAAG
GATTTTGAAGAGTGTGCACATAAATTATTGAAATTAAAGATTCAAGGTCATGAAGATGAA
ATTTGTAATATGCTAATTCAATGTTGTAGTCAAGAGAGAACCTATTTACCATTCTATGGT
AATTTATCACAACGTTTCTGTATGATCAATAAGATCTATCGTGACTTGTTTGATCGTTGT
TTTGCTGAACAATACGCTGTCATTCATAGATTGGAGACTAACAGATTTAGAAATATTGCA
AAGATGTATGCTCATCTATTCTTTACCGATGCTTTACCTTGGACAACGTTACAGTATGTT
CATATCAATGAAAATGAAACTAATAGTGCAAGTCGTATCTTTATAAAAATACTCTTTCAA
GAGATGGCAGAGTTTTTAGGTATTCAAAAATTAAATGAACGTTTACAAGATCCTTCACTT
CAAGAATCTTTCACTGGTATATTTCCAAAAGATAATCAAAAGAATACAAGATATGCTATC
AACTTCTTTACTTCAATTAGTTTAGGTGCTTTAACTCATGATTTAAGAGAATTCTTAAAA
AATGCTCCAAAAGTTTCTGTAATTGAAAAGAAAAAACAAGATTCAAGTAGTGATGATAGT
AGTAGTGATGATAGTAGTGATGATAGTAGTGATGACAGTAGTGATGACAGTAGTGATAGT
AGTGATAGTAGTAGTAGTGATGATAGTAGTAGGAAGANAAAGAAGAAGAAAAAAGAGAAG
AAAGTAATAATAAAAAATTAAATTAAATTCCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
kysplsphinknvk*rw*NYKPPISKXAGILNMIVEEKEIIKIHMIINQKTXERSKSRSR
SRDRDRNRYDRDRERDLEKERERDRDHDRNRDRDRDRDRNRDRDRDHDRNRDRDRNRDRD
HDRDRDREREKDRERDRDRDRYGNKERDRDRDRERDRDRERDREREREKERERERERDRD
RYGDRSTSR---

---LAIMSSKDFEECAHKLLKLKIQGHEDEICNMLIQCCSQERTYLPFYGNLSQRFCMIN
KIYRDLFDRCFAEQYAVIHRLETNRFRNIAKMYAHLFFTDALPWTTLQYVHINENETNSA
SRIFIKILFQEMAEFLGIQKLNERLQDPSLQESFTGIFPKDNQKNTRYAINFFTSISLGA
LTHDLREFLKNAPKVSVIEKKKQDSSSDDSSSDDSSDDSSDDSSDDSSDSSDSSSSDDSS
RKXKKKKKEKKVIIKN*ikfq
Translated Amino Acid sequence (All Frames) 
Frame A:
kysplsphinknvk*rw*NYKPPISKXAGILNMIVEEKEIIKIHMIINQKTXERSKSRSR
SRDRDRNRYDRDRERDLEKERERDRDHDRNRDRDRDRDRNRDRDRDHDRNRDRDRNRDRD
HDRDRDREREKDRERDRDRDRYGNKERDRDRDRERDRDRERDREREREKERERERERDRD
RYGDRSTSR---

---i*qlcqvrilksvhiny*n*rfkvmkmkfvic*fnvvvkrepiyhsmviyhnvsv*s
irsivtclivvllnntlsfidwrltdleilqrcmliyslpmlylgqrysmfismkmkliv
qvvsl*kysfkrwqsf*vfkn*mnvykilhfknlslvyfqkiikriqdmlstsllqlv*v
l*lmi*ens*kmlqkfl*lkrknkiqvvmivvvmivvmivvmtvvmtvvivvivvvvmiv
vgrxrrrkkrrk***kiklns

Frame B:
nihlfphi*ikmsnedgeiishqyqxrqgf*i***krkrl*rfi***ikrqxkevkveve
vgieieidtieiekei*kkkekeietmteieieietvteieieiettteieieieietvt
mtvieigkekktekeieieidtvtkkeigigiekeieiekgiekekekkkekekekeiei
dmvidqpq---

---fsnyvk*gf*rvct*iieikdsrs*r*nl*yansml*srenlftilw*fittflydq
*dls*lv*slfc*tirchs*igd*qi*kyckdvcssilyrcftldnvtvcsyq*k*n**c
ksylykntlsrdgrvfryskik*tftrsftsrifhwyiskr*skeykicyqllyfn*frc
fns*fkrilkkcsksfcn*kektrfk****************q***q*************
*exkeeekreesnnkkln*ip

Frame C:
iftsfptyk*kcqmkmvkl*atnikxgrdseydsrrerdykdsydnkskdxrkk*k*k*k
*g*r*k*ir*r*rkrfrkrkrkr*rp*qk*r*r*rp*qk*r*r*rprqk*r*r*k*rp*p
*p**r*gkrkrqrkr*r*r*ir*qrkr*g*g*rkr*r*rkg*rkrkrkrkrkrkrkr*r*
iw**inlk---

---LAIMSSKDFEECAHKLLKLKIQGHEDEICNMLIQCCSQERTYLPFYGNLSQRFCMIN
KIYRDLFDRCFAEQYAVIHRLETNRFRNIAKMYAHLFFTDALPWTTLQYVHINENETNSA
SRIFIKILFQEMAEFLGIQKLNERLQDPSLQESFTGIFPKDNQKNTRYAINFFTSISLGA
LTHDLREFLKNAPKVSVIEKKKQDSSSDDSSSDDSSDDSSDDSSDDSSDSSDSSSSDDSS
RKXKKKKKEKKVIIKN*ikfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK247 (SHK247Q) /CSM/SH/SHK2-B/SHK247Q.Seq.d/                       1213   0.0
SHE516 (SHE516Q) /CSM/SH/SHE5-A/SHE516Q.Seq.d/                       1205   0.0
AHO219 (AHO219Q) /CSM/AH/AHO2-A/AHO219Q.Seq.d/                       1205   0.0
AHE848 (AHE848Q) /CSM/AH/AHE8-B/AHE848Q.Seq.d/                       1205   0.0
AHD458 (AHD458Q) /CSM/AH/AHD4-C/AHD458Q.Seq.d/                       1205   0.0
AHD241 (AHD241Q) /CSM/AH/AHD2-B/AHD241Q.Seq.d/                       1205   0.0
AHC821 (AHC821Q) /CSM/AH/AHC8-A/AHC821Q.Seq.d/                       1205   0.0
SHA246 (SHA246Q) /CSM/SH/SHA2-B/SHA246Q.Seq.d/                       1203   0.0
AHM478 (AHM478Q) /CSM/AH/AHM4-D/AHM478Q.Seq.d/                       1203   0.0
SHB812 (SHB812Q) /CSM/SH/SHB8-A/SHB812Q.Seq.d/                       1197   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CO979316|CO979316.1 GM89004B1F06.r1 Gm-r1089 Glycine max cDNA clone Gm-r1089-1427 3, mRNA sequence.    44   8e-04  2
BP505674|BP505674.1 Hydra magnipapillata cDNA, clone:hm_03511.     38   0.13   2
AC153337|AC153337.2 Loxodonta africana clone VMRC15-247J19, WORKING DRAFT SEQUENCE, 4 ordered pieces.    50   0.14   1
CZ952175|CZ952175.1 263798 Tomato EcoRI BAC Library Lycopersicon esculentum genomic clone SL_EcoRI0054G21 5, genomic survey sequence.    36   1.4    3
CW859954|CW859954.1 shS2kd34-21.b_022.ab1 Whole-genome shotgun library of the elephant shark (aka elephant fish) Callorhinchus milii genomic, genomic survey sequence.    34   1.5    2
AV551645|AV551645.1 Arabidopsis thaliana cDNA clone:RZ129e06R, 5' end.    36   1.7    2
AC090332|AC090332.5 Homo sapiens chromosome 18 clone RP11-833L9 map 18, WORKING DRAFT SEQUENCE, 9 unordered pieces.    46   2.2    1
AP001177|AP001177.3 Homo sapiens genomic DNA, chromosome 18q11.2, clone:RP11-799B12, WORKING DRAFT SEQUENCE, 16 unordered pieces.    46   2.2    1
AC166567|AC166567.1 Spironucleus vortens clone JGIBAWF-9N7, WORKING DRAFT SEQUENCE, 3 unordered pieces.    46   2.2    1
AP001164|AP001164.2 Homo sapiens genomic DNA, chromosome 18q11.2 clone:RP11-833L9, WORKING DRAFT SEQUENCE, 40 unordered pieces.    46   2.2    1
dna update 2006. 6. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116949_6(AC116949|pid:none) Oryza sativa (japonica cultivar-gr...   269   2e-70
AP008217_259(AP008217|pid:none) Oryza sativa (japonica cultivar-...   269   2e-70
AM778050_1(AM778050|pid:none) Oryza sativa (japonica cultivar-gr...   268   4e-70
AM778049_1(AM778049|pid:none) Oryza sativa Indica Group mRNA for...   268   4e-70
AK073908_1(AK073908|pid:none) Oryza sativa Japonica Group cDNA c...   268   4e-70
AM778052_1(AM778052|pid:none) Oryza sativa (indica cultivar-grou...   268   4e-70
EU970885_1(EU970885|pid:none) Zea mays clone 353096 unknown mRNA.     267   5e-70
CP001577_135(CP001577|pid:none) Micromonas sp. RCC299 chromosome...   254   6e-66
AK129404_1(AK129404|pid:none) Mus musculus premature mRNA for mK...   248   5e-64
(Q8C5N3) RecName: Full=Nucampholin homolog;  &AK077961_1(AK07796...   247   6e-64
protein update 2009. 5.29
PSORT 

 psg: 0.89  gvh: 0.17  alm: 0.42  top: 0.33  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.20  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHK247 is nuc
5' end seq. ID SHK247F
5' end seq. 
>SHK247F.Seq
AAATATTCACCTCTTTCCCCACATATAAATAAAAATGTCAAATGAAGATGGTGAAATTAT
AAGCCACCAATATCAAAGNAGGCAGGGATTCTGAATATGATAGTAGAAGAGAAAGAGATT
ATAAAGATTCATATGATAATAAATCAAAAGACAGNAGAAAGAAGTAAAAGTAGAAGTAGA
AGTAGGGATAGAGATAGAAATAGATACGATAGAGATAGAGAAAGAGATTTAGAAAAAGAA
AGAGAAAGAGATAGAGACCATGACAGAAATAGAGATAGAGATAGAGACCGTGACAGAAAT
AGAGATAGAGATAGAGACCACGACAGAAATAGAGATAGAGATAGAAATAGAGACCGTGAC
CATGACCGTGATAGAGATAGGGAAAGAGAAAAAGACAGAGAAAGAGATAGAGATAGAGAT
AGATACGGTAACAAAGAAAGAGATAGGGATAGGGATAGAGAAAGAGATAGAGATAGAGAA
AGGGATAGAGAAAGAGAAAGAGAAAAAGAAAGAGAAAGAGAAAGAGAAAGAGATAGAGAT
AGATATGGTGATAGATCAACCTCAAGANNNNNNNNNN
Length of 5' end seq. 577
3' end seq. ID SHK247Z
3' end seq. 
>SHK247Z.Seq
NNNNNNNNNNATTTAGCAATTATGTCAAGTAAGGATTTTGAAGAGTGTGCACATAAATTA
TTGAAATTAAAGATTCAAGGTCATGAAGATGAAATTTGTAATATGCTAATTCAATGTTGT
AGTCAAGAGAGAACCTATTTACCATTCTATGGTAATTTATCACAACGTTTCTGTATGATC
AATAAGATCTATCGTGACTTGTTTGATCGTTGTTTTGCTGAACAATACGCTGTCATTCAT
AGATTGGAGACTAACAGATTTAGAAATATTGCAAAGATGTATGCTCATCTATTCTTTACC
GATGCTTTACCTTGGACAACGTTACAGTATGTTCATATCAATGAAAATGAAACTAATAGT
GCAAGTCGTATCTTTATAAAAATACTCTTTCAAGAGATGGCAGAGTTTTTAGGTATTCAA
AAATTAAATGAACGTTTACAAGATCCTTCACTTCAAGAATCTTTCACTGGTATATTTCCA
AAAGATAATCAAAAGAATACAAGATATGCTATCAACTTCTTTACTTCAATTAGTTTAGGT
GCTTTAACTCATGATTTAAGAGAATTCTTAAAAAATGCTCCAAAAGTTTCTGTAATTGAA
AAGAAAAAACAAGATTCAAGTAGTGATGATAGTAGTAGTGATGATAGTAGTGATGATAGT
AGTGATGACAGTAGTGATGACAGTAGTGATAGTAGTGATAGTAGTAGTAGTGATGATAGT
AGTAGGAAGANAAAGAAGAAGAAAAAAGAGAAGAAAGTAATAATAAAAAATTAAATTAAA
TTCCAA
Length of 3' end seq. 786
Connected seq. ID SHK247P
Connected seq. 
>SHK247P.Seq
AAATATTCACCTCTTTCCCCACATATAAATAAAAATGTCAAATGAAGATGGTGAAATTAT
AAGCCACCAATATCAAAGNAGGCAGGGATTCTGAATATGATAGTAGAAGAGAAAGAGATT
ATAAAGATTCATATGATAATAAATCAAAAGACAGNAGAAAGAAGTAAAAGTAGAAGTAGA
AGTAGGGATAGAGATAGAAATAGATACGATAGAGATAGAGAAAGAGATTTAGAAAAAGAA
AGAGAAAGAGATAGAGACCATGACAGAAATAGAGATAGAGATAGAGACCGTGACAGAAAT
AGAGATAGAGATAGAGACCACGACAGAAATAGAGATAGAGATAGAAATAGAGACCGTGAC
CATGACCGTGATAGAGATAGGGAAAGAGAAAAAGACAGAGAAAGAGATAGAGATAGAGAT
AGATACGGTAACAAAGAAAGAGATAGGGATAGGGATAGAGAAAGAGATAGAGATAGAGAA
AGGGATAGAGAAAGAGAAAGAGAAAAAGAAAGAGAAAGAGAAAGAGAAAGAGATAGAGAT
AGATATGGTGATAGATCAACCTCAAGA----------ATTTAGCAATTATGTCAAGTAAG
GATTTTGAAGAGTGTGCACATAAATTATTGAAATTAAAGATTCAAGGTCATGAAGATGAA
ATTTGTAATATGCTAATTCAATGTTGTAGTCAAGAGAGAACCTATTTACCATTCTATGGT
AATTTATCACAACGTTTCTGTATGATCAATAAGATCTATCGTGACTTGTTTGATCGTTGT
TTTGCTGAACAATACGCTGTCATTCATAGATTGGAGACTAACAGATTTAGAAATATTGCA
AAGATGTATGCTCATCTATTCTTTACCGATGCTTTACCTTGGACAACGTTACAGTATGTT
CATATCAATGAAAATGAAACTAATAGTGCAAGTCGTATCTTTATAAAAATACTCTTTCAA
GAGATGGCAGAGTTTTTAGGTATTCAAAAATTAAATGAACGTTTACAAGATCCTTCACTT
CAAGAATCTTTCACTGGTATATTTCCAAAAGATAATCAAAAGAATACAAGATATGCTATC
AACTTCTTTACTTCAATTAGTTTAGGTGCTTTAACTCATGATTTAAGAGAATTCTTAAAA
AATGCTCCAAAAGTTTCTGTAATTGAAAAGAAAAAACAAGATTCAAGTAGTGATGATAGT
AGTAGTGATGATAGTAGTGATGATAGTAGTGATGACAGTAGTGATGACAGTAGTGATAGT
AGTGATAGTAGTAGTAGTGATGATAGTAGTAGGAAGANAAAGAAGAAGAAAAAAGAGAAG
AAAGTAATAATAAAAAATTAAATTAAATTCCAA
Length of connected seq. 1343
Full length Seq ID -
Full length Seq. -
Length of full length seq. -