>SHK244 (SHK244Q) /CSM/SH/SHK2-B/SHK244Q.Seq.d/
ACTGTTGGCCTACTGGGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATC
TCTTCTCCAGTTGATACTTCNAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACT
TTACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTT
ACATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGA
ATCACTTCAAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAACCACCACAA
GATTCAACTCCAGTGGTAGATATTAAAGCTCCAAATGTTTTAGATTCATGTAGTCCATTG
GTATTAGATGCTAGTCATTCAAAAGGTGGTAATGGAAAAGCATTAACCTATGATTGGTCG
GTTAGTGGGGCAGATACAAACACTCAATTAAATAATCTATTGGATCAAAATAGTGCAACC
ATAACAATTCCAAATGATCTTTTGTCATCTGGTCAAACTTATACTTTTACACTTAAAATC
ACAAATTGGTTAAAGAAATCTGATAGCGCAAGTGTTTCAGTAACAAAGAAAGAGGGTGAA
GTTTTAGATGTAACAATXXXXXXXXXXCTACATATGGAATTAAGTAAGAGTGGTCAATCG
ATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACT
CCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAA
ATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAA
ACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATC
AATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAA
GAATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCA
ACCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTA
ATTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGT
GTTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAA
AACAAAAAGAACAAATCAAAGAAAACCAAAGAACAAATCAACAAAGAAAAAGAAGACAAA
AAGAAACAAGCAAAAGAAAAGAAAGAAG

TVGLLGALGANNGNFFSCDISSPVDTSNRPSCSSLFSSATLQTLGSSPRCEYTASNILMV
TLGSGESVSVGQQLEFSQGGITSKDGSKNNYQTITLEPPQDSTPVVDIKAPNVLDSCSPL
VLDASHSKGGNGKALTYDWSVSGADTNTQLNNLLDQNSATITIPNDLLSSGQTYTFTLKI
TNWLKKSDSASVSVTKKEGEVLDVT---

---LHMELSKSGQSIGFKKDDEGQSLDGEKLAFTPKFSIPSSVLSKNKLKQTSEITGKLI
TVPSNIHFNHKSDQTIFPKTVSLELFNGGSQLSINGLKDQIIIDMGKHSPPKDKEFKCGW
WSSNKKEWLTDGCSTHKVDGNIQCKCNHLTEFGLIEFAKSSQNIWIIIGPVVGGVVFAAA
MVGTVVLVKRKKNKNKKNKSKKTKEQINKEKEDKKKQAKEKKE

Frame A:
TVGLLGALGANNGNFFSCDISSPVDTSNRPSCSSLFSSATLQTLGSSPRCEYTASNILMV
TLGSGESVSVGQQLEFSQGGITSKDGSKNNYQTITLEPPQDSTPVVDIKAPNVLDSCSPL
VLDASHSKGGNGKALTYDWSVSGADTNTQLNNLLDQNSATITIPNDLLSSGQTYTFTLKI
TNWLKKSDSASVSVTKKEGEVLDVT---

---LHMELSKSGQSIGFKKDDEGQSLDGEKLAFTPKFSIPSSVLSKNKLKQTSEITGKLI
TVPSNIHFNHKSDQTIFPKTVSLELFNGGSQLSINGLKDQIIIDMGKHSPPKDKEFKCGW
WSSNKKEWLTDGCSTHKVDGNIQCKCNHLTEFGLIEFAKSSQNIWIIIGPVVGGVVFAAA
MVGTVVLVKRKKNKNKKNKSKKTKEQINKEKEDKKKQAKEKKE

Frame B:
llaywvhsvqtmvislvvisllqlilxiglhvvlfsllqlyklwvvlqdvnillqtf*wl
hwvvvkvyqlannwnflkveslqkmvvriiikl*hwnhhkiqlqw*ilklqmf*ihvvhw
y*mlviqkvvmekh*pmigrlvgqiqtln*iiywikivqp*qfqmifchlvklillhlks
qig*rnliaqvfq*qrkrvkf*m*q---

---yiwn*vrvvnrlvskrmmkvnllmvknllslqnsvfhqvfyqrin*nkplkslvn*l
qfhqifisiinlikpfsqklfhlnfsmvalnyqsmalkiklsliwenihhqkiknlnvvg
gapikkng*qmvvqpik*mvifnvnvtilqslv*lnlqnplktfgslldq*lvvlylqlq
wlvllyw*rerktktkrtnqrkpknkstkkkktkrnkqkkrkk

Frame C:
cwptgctrckqw*fl*l*ylfss*yfx*afm*fsflfcnftnfg*fskm*iycfkhsngy
ig*w*kciswptigifsrwnhfkrw**e*lsnynigtttrfnssgry*sskcfrfm*sig
irc*sfkrw*wksinl*lvg*wgrykhsik*sigsk*cnhnnsk*sfviwsnlyfyt*nh
klvkei**rkcfsnkerg*sfrcnn---

---tygik*ewsidwfqkg**rsis*w*ktcfhskiqysikcfike*iktnl*nhw*tny
ssikysfqs*i*snhfpkncft*tfqwwlsiinqwp*rsnyh*ygktfttkr*ri*mwlv
elq*krmvnrwlfnp*srw*ysm*m*psyrvwfn*ickilskhldhywtsswwccicscn
gwyccigkekekqkqkeqikenqrtnqqrkrrqketskrker

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK244 (SHK244Q) /CSM/SH/SHK2-B/SHK244Q.Seq.d/                       2276   0.0
SHH523 (SHH523Q) /CSM/SH/SHH5-A/SHH523Q.Seq.d/                       1162   0.0
CHG850 (CHG850Q) /CSM/CH/CHG8-C/CHG850Q.Seq.d/                       1154   0.0
CHG594 (CHG594Q) /CSM/CH/CHG5-D/CHG594Q.Seq.d/                       1152   0.0
SHC748 (SHC748Q) /CSM/SH/SHC7-B/SHC748Q.Seq.d/                       1146   0.0
CHP795 (CHP795Q) /CSM/CH/CHP7-D/CHP795Q.Seq.d/                       1146   0.0
SHK142 (SHK142Q) /CSM/SH/SHK1-B/SHK142Q.Seq.d/                       1144   0.0
SHA470 (SHA470Q) /CSM/SH/SHA4-C/SHA470Q.Seq.d/                       1140   0.0
SHB693 (SHB693Q) /CSM/SH/SHB6-D/SHB693Q.Seq.d/                       1138   0.0
SHF373 (SHF373Q) /CSM/SH/SHF3-D/SHF373Q.Seq.d/                       1134   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CJ484472|CJ484472.1 Macaca fascicularis mRNA, clone: QtsA-12015, 5' end, expressed intestis.    48   0.52   1
CJ477353|CJ477353.1 Macaca fascicularis mRNA, clone: QtrA-12165, 5' end, expressed in brain temporal lobe.    48   0.52   1
CJ470588|CJ470588.1 Macaca fascicularis mRNA, clone: QorA-10120, 5' end, expressed in brain occipital lobe.    48   0.52   1
AC024453|AC024453.2 Homo sapiens chromosome 4 clone RP11-2B17 map 4, WORKING DRAFT SEQUENCE, 51 unordered pieces.    48   0.52   1
AC013774|AC013774.3 Homo sapiens clone RP11-10P1, WORKING DRAFT SEQUENCE, 11 unordered pieces.    48   0.52   1
AC103541|AC103541.6 Homo sapiens BAC clone RP11-818M13 from 4, complete sequence.    48   0.52   1
AC097518|AC097518.2 Homo sapiens BAC clone RP11-487E13 from 4, complete sequence.    48   0.52   1
AJ410884|AJ410884.1 Influenza A virus (swine/Cotes d'Armor/1515/99 (H1N1)) partial N1NA  gene for Neuraminidase, genomic RNA.    42   0.95   2
AJ410883|AJ410883.1 Influenza A virus (swine/Cotes d'Armor/1482/99 (H1N1)) partial N1NA  gene for Neuraminidase, genomic RNA.    42   0.97   2
CJ477883|CJ477883.1 Macaca fascicularis mRNA, clone: QtrA-12820, 5' end, expressed in brain temporal lobe.    46   2.1    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001332_186(CP001332|pid:none) Micromonas sp. RCC299 chromosome...    67   2e-09
EF203419_1(EF203419|pid:none) Strongylocentrotus purpuratus rece...    66   3e-09
AK154648_1(AK154648|pid:none) Mus musculus NOD-derived CD11c +ve...    50   2e-04
(Q8R0T6) RecName: Full=Probable G-protein coupled receptor 97; F...    50   2e-04
AK037149_1(AK037149|pid:none) Mus musculus 6 days neonate skin c...    50   2e-04
AB041939_1(AB041939|pid:none) Homo sapiens KPG_006 mRNA for G-pr...    50   2e-04
AF216967_1(AF216967|pid:none) Homo sapiens putative vascular ind...    48   6e-04
(Q8K3V3) RecName: Full=G-protein coupled receptor 56; Flags: Pre...    47   0.002
AK295345_1(AK295345|pid:none) Homo sapiens cDNA FLJ55998 complet...    46   0.003
AF166382_1(AF166382|pid:none) Mus musculus serpentine receptor (...    45   0.004

 psg: 0.92  gvh: 0.59  alm: 0.22  top: 1.17  tms: 0.07  mit: 0.17  mip: 0.00
 nuc: 0.17  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vesicles of secretory system
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for SHK244 is cyt

>SHK244F.Seq
ACTGTTGGCCTACTGGGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATC
TCTTCTCCAGTTGATACTTCNAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACT
TTACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTT
ACATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGA
ATCACTTCAAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAACCACCACAA
GATTCAACTCCAGTGGTAGATATTAAAGCTCCAAATGTTTTAGATTCATGTAGTCCATTG
GTATTAGATGCTAGTCATTCAAAAGGTGGTAATGGAAAAGCATTAACCTATGATTGGTCG
GTTAGTGGGGCAGATACAAACACTCAATTAAATAATCTATTGGATCAAAATAGTGCAACC
ATAACAATTCCAAATGATCTTTTGTCATCTGGTCAAACTTATACTTTTACACTTAAAATC
ACAAATTGGTTAAAGAAATCTGATAGCGCAAGTGTTTCAGTAACAAAGAAAGAGGGTGAA
GTTTTAGATGTAACAATNNNNNNNNNN

>SHK244Z.Seq
NNNNNNNNNNCTACATATGGAATTAAGTAAGAGTGGTCAATCGATTGGTTTCAAAAAGGA
TGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACTCCAAAATTCAGTATTCC
ATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAAATCACTGGTAAACTAAT
TACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAAACCATTTTCCCAAAAAC
TGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATCAATGGCCTTAAAGATCA
AATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAAGAATTTAAATGTGGTTG
GTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCAACCCATAAAGTAGATGG
TAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTAATTGAATTTGCAAAATC
CTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGC
AATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAAAACAAAAAGAACAAATC
AAAGAAAACCAAAGAACAAATCAACAAAGAAAAAGAAGACAAAAAGAAACAAGCAAAAGA
AAAGAAAGAAG

>SHK244P.Seq
ACTGTTGGCCTACTGGGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATC
TCTTCTCCAGTTGATACTTCNAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACT
TTACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTT
ACATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGA
ATCACTTCAAAAGATGGTAGTAAGAATAATTATCAAACTATAACATTGGAACCACCACAA
GATTCAACTCCAGTGGTAGATATTAAAGCTCCAAATGTTTTAGATTCATGTAGTCCATTG
GTATTAGATGCTAGTCATTCAAAAGGTGGTAATGGAAAAGCATTAACCTATGATTGGTCG
GTTAGTGGGGCAGATACAAACACTCAATTAAATAATCTATTGGATCAAAATAGTGCAACC
ATAACAATTCCAAATGATCTTTTGTCATCTGGTCAAACTTATACTTTTACACTTAAAATC
ACAAATTGGTTAAAGAAATCTGATAGCGCAAGTGTTTCAGTAACAAAGAAAGAGGGTGAA
GTTTTAGATGTAACAAT----------CTACATATGGAATTAAGTAAGAGTGGTCAATCG
ATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAAAACTTGCTTTCACT
CCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAAAACAAACCTCTGAA
ATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATCATAAATCTGATCAA
ACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTGGCTCTCAATTATCAATC
AATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATTCACCACCAAAAGATAAA
GAATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGTTAACAGATGGTTGTTCA
ACCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATCTTACAGAGTTTGGTTTA
ATTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTGGACCAGTAGTTGGTGGT
GTTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAAAGAGAAAGAAAAACAAA
AACAAAAAGAACAAATCAAAGAAAACCAAAGAACAAATCAACAAAGAAAAAGAAGACAAA
AAGAAACAAGCAAAAGAAAAGAAAGAAG