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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK230
Library SH
(Link to library)
Clone ID SHK230
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16475-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>SHK230 (SHK230Q) /CSM/SH/SHK2-B/SHK230Q.Seq.d/
XXXXXXXXXXTGTGTAGCTCAAGATTCTCATTGTGATGATGGTAATCCATGTACTGATAA
TGCTTGTGTCGCTTCAGCTAATAGTACCGCCCAATAGCCAAGTTGGTCAATGCACTCAAA
CTCCAACTGCTAAATGTGATTTGGGTGATAAATGTATGGTTTACAGTTGTACCTTGGAAG
GTGGTTGTAATGCAACTCAACGTGTTTGTCCAAACTCTGGTGCTTGTAAAATTGGTTATT
GTGAACCTGGTTTCGGTTGTAAATTAAAGGATAGAGTTTGTTCTTCCGATTGGTATTGTA
TTGAAGCTCAATGTGATGCAAAATATGGTTGCATTCAATTCGATAGAAGATGTGCTCCTG
ATAATAGTAAATGTCAAGAAGGTGTCTGTGTAAACAATACTGCTATCGGTAAAAATGATG
GAAAATGTATTTCTGAAGATTATGATCCAAAACCATTTGTTTGTCAAAAAGCTGCTTTAG
TTTCAACTGCTGTTATTGCAAGTGTTGTTGTAGTTGGTGCTGTTGTTTTAGGTGCTGCTA
TATTTGCGGGTAAGAAAGGTTATGATGCTTGGAAAACAAGTCAAGGAAATGTTATGGCAG
CTTCACAAGCTAATCCATTATATACTCAATCAAGTAATGGAGGTGAAAATCCACTCTATA
ACTCACC
sequence update 2002. 9.10
Translated Amino Acid sequence 
---v*LKILIVMMVIHVLIMLVSLQLIVPPNSQVGQCTQTPTAKCDLGDKCMVYSCTLEG
GCNATQRVCPNSGACKIGYCEPGFGCKLKDRVCSSDWYCIEAQCDAKYGCIQFDRRCAPD
NSKCQEGVCVNNTAIGKNDGKCISEDYDPKPFVCQKAALVSTAVIASVVVVGAVVLGAAI
FAGKKGYDAWKTSQGNVMAASQANPLYTQSSNGGENPLYNS
Translated Amino Acid sequence (All Frames) 
Frame A:
---v*LKILIVMMVIHVLIMLVSLQLIVPPNSQVGQCTQTPTAKCDLGDKCMVYSCTLEG
GCNATQRVCPNSGACKIGYCEPGFGCKLKDRVCSSDWYCIEAQCDAKYGCIQFDRRCAPD
NSKCQEGVCVNNTAIGKNDGKCISEDYDPKPFVCQKAALVSTAVIASVVVVGAVVLGAAI
FAGKKGYDAWKTSQGNVMAASQANPLYTQSSNGGENPLYNS


Frame B:
---cssrfsl**w*smy**clcrfs**yrpiaklvnalklqllnviwvinvwftvvpwkv
vvmqlnvfvqtlvlvklvivnlvsvvn*riefvlpigivlklnvmqnmvafnsiedvlli
ivnvkkvsv*tillsvkmmenvflkimiqnhlfvkkll*fqllllqvll*lvllf*vlly
lrvrkvmmlgkqvkemlwqlhklihyilnqvmevkihsith


Frame C:
---vaqdshcddgnpctdnacvasanstaq*pswsmhsnsnc*m*fg**myglqlylgrw
l*cnstclsklwcl*nwll*twfrl*ikg*slffrlvly*ssm*ckiwlhsir*kmcs**
**msrrclckqycyr*k*wkmyf*rl*skticlskscfsfnccyckcccswcccfrccyi
cg*erl*clenksrkcygsfts*siiysik*wr*kstl*lt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK230 (SHK230Q) /CSM/SH/SHK2-B/SHK230Q.Seq.d/                       1302   0.0
SHL876 (SHL876Q) /CSM/SH/SHL8-D/SHL876Q.Seq.d/                       1287   0.0
SHG146 (SHG146Q) /CSM/SH/SHG1-B/SHG146Q.Seq.d/                       1287   0.0
SHG135 (SHG135Q) /CSM/SH/SHG1-B/SHG135Q.Seq.d/                       1287   0.0
SHF677 (SHF677Q) /CSM/SH/SHF6-D/SHF677Q.Seq.d/                       1287   0.0
SHF156 (SHF156Q) /CSM/SH/SHF1-C/SHF156Q.Seq.d/                       1287   0.0
SHE102 (SHE102Q) /CSM/SH/SHE1-A/SHE102Q.Seq.d/                       1287   0.0
SHC233 (SHC233Q) /CSM/SH/SHC2-B/SHC233Q.Seq.d/                       1287   0.0
SFJ316 (SFJ316Q) /CSM/SF/SFJ3-A/SFJ316Q.Seq.d/                       1287   0.0
SFI733 (SFI733Q) /CSM/SF/SFI7-B/SFI733Q.Seq.d/                       1287   0.0
own update 2002.12. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strainAX4, complete sequence.    66   1e-06  1
AJ548837|AJ548837.1 Dictyostelium discoideum mRNA for extracellular signalling molecule  DicA (dicA gene).    60   4e-05  2
AJ548838|AJ548838.1 Dictyostelium discoideum dicA gene for extracellular signalling  molecule DicA, exons 1-2.    60   5e-05  2
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    42   0.002  4
CX053834|CX053834.1 UCRCS09_8E08_g Ruby Orange Developing Seed cDNA Library UCRCS09 Citrus sinensis cDNA clone UCRCS09-8E08-I15-1-5.g, mRNA sequence.    50   0.067  1
CC946427|CC946427.1 BOIFV20TF BO_1.4_1.6_KB_nuc Brassica oleracea genomic clone BOIFV20, genomic survey sequence.    50   0.067  1
CC075688|CC075688.1 CSU-K33r.25G4.SP6 CSU-K33r Aedes aegypti genomic clone CSU-K33r.25G4, DNA sequence.    50   0.067  1
BH960705|BH960705.1 odd88f07.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    50   0.067  1
AZ541534|AZ541534.1 ENTGE45TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.067  1
AR549621|AR549621.1 Sequence 4752 from patent US 6747137.          46   0.27   2
dna update 2006. 6. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                357   e-110
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   169   1e-42
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   162   2e-41
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   154   7e-39
(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                146   1e-34
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                118   3e-26
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                118   1e-25
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    59   6e-10
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...    54   4e-09
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...    52   1e-07
protein update 2009. 5.29
PSORT 

 psg: 1.00  gvh: 0.58  alm: 0.13  top: 0.40  tms: 0.13  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 28.0 %: endoplasmic reticulum
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: cytoplasmic
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for SHK230 is mit
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SHK230Z
3' end seq. 
>SHK230Z.Seq
NNNNNNNNNNTGTGTAGCTCAAGATTCTCATTGTGATGATGGTAATCCATGTACTGATAA
TGCTTGTGTCGCTTCAGCTAATAGTACCGCCCAATAGCCAAGTTGGTCAATGCACTCAAA
CTCCAACTGCTAAATGTGATTTGGGTGATAAATGTATGGTTTACAGTTGTACCTTGGAAG
GTGGTTGTAATGCAACTCAACGTGTTTGTCCAAACTCTGGTGCTTGTAAAATTGGTTATT
GTGAACCTGGTTTCGGTTGTAAATTAAAGGATAGAGTTTGTTCTTCCGATTGGTATTGTA
TTGAAGCTCAATGTGATGCAAAATATGGTTGCATTCAATTCGATAGAAGATGTGCTCCTG
ATAATAGTAAATGTCAAGAAGGTGTCTGTGTAAACAATACTGCTATCGGTAAAAATGATG
GAAAATGTATTTCTGAAGATTATGATCCAAAACCATTTGTTTGTCAAAAAGCTGCTTTAG
TTTCAACTGCTGTTATTGCAAGTGTTGTTGTAGTTGGTGCTGTTGTTTTAGGTGCTGCTA
TATTTGCGGGTAAGAAAGGTTATGATGCTTGGAAAACAAGTCAAGGAAATGTTATGGCAG
CTTCACAAGCTAATCCATTATATACTCAATCAAGTAATGGAGGTGAAAATCCACTCTATA
ACTCACC
Length of 3' end seq. 667
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -