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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK214
Library SH
(Link to library)
Clone ID SHK214
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SHK214P
(Link to Original site)
Representative DNA sequence 
>SHK214 (SHK214Q) /CSM/SH/SHK2-A/SHK214Q.Seq.d/
TTATTATTTTTATTAATATTAATAATTTTCTTTTATTAATTATTATAATAGTTTTCTTTT
TTTTTTTTNATAATTTTTTATTTATAANGTTNAATAATAATAATAATAATAATAATAATA
ATAATATNCNTTTTNCCAXXXXXXXXXXTAAAAGACATATCAAATTAATCAACTCTGTTG
CCGAGGTTATCAACCGTGATAAAGAGGTCGATCAATACCTCAAGGGTTGTTTTCATTGCA
AATTACANCGTTTCAATCGCTCAAGTCATCGTTCCAGCCTCTGATATCAATCAACAAATT
TCAACCGCTGGGTACCGAAGCCTNTGGTANTAGTAACATGAAATTCACTATGAATGGTAG
TTTAATCATTGGTACTTTGGATGGTGCAAATGTTGAAATCGCCGAAGAAGTTGGTCAAGA
GAATATGTTTATCTTTGGTTTACGTACAAGTGAAGTTGAAGCTGNTCGTGAAAAAATGAC
AAACAAAGAAGTCAACATTGACCCACGTNTTCAAGAGGTATTCCTTAATATTGAATTAGG
TACATTTGGTCCACCAGATGTTTTCAGACCAATCTTGGACTCTCCTAATCTTTAGTGATT
TCTACTTAAGCATTCAAGATTTCCCACTCTACCTTGACTCTCAAGCTTCAGTTGATGAAT
TATGGAAAGATCAAAGTGCTTGGGTTAAAAAATCAATCATTAACAGTGCTTCAACTTATT
TCTTCTCTTCTGATAGAGCTATGAATGAATATGCTGAACAAATTTGGGATATTAAACCAT
GGAAGTNAAACTACTTTAAATAGAAGATATTAANAAAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
IIFININNFLLLIIIIVFFFFFXNFLFIXXNNNNNNNNNNNXXFX---

---KRHIKLINSVAEVINRDKEVDQYLKGCFHCKLXRFNRSSHRSSL*yqstnfnrwvpk
pxvxvt*nsl*mvv*slvlwmvqmlkspkklvkriclslvyvqvklklxvkk*qtkkstl
thvfkrysliln*vhlvhqmfsdqswtllifsdfylsiqdfplyldsqasvdelwkdqsa
wvkksiinsastyffssdramneyaeqiwdikpwkxnyfk*kilxk*
Translated Amino Acid sequence (All Frames) 
Frame A:
llflliliiffy*ll**fsfffxiifyl*xxiiiiiiiiiiixxxp---

---*ktyqinqlccrgyqp**rgrsipqglfslqitxfqslkssfqplisinkfqplgte
axgxsnmkftmngsliigtldganveiaeevgqenmfifglrtseveaxrekmtnkevni
dprxqevflnielgtfgppdvfrpildspnl**fllkhsrfptlp*lssfs**imerskc
lg*kinh*qcfnlfllf**sye*ic*tnlgy*tmevkll*iedixki

Frame B:
yyfy*y**fsfinyynsflfffx*ffiyxvx**********yxfx---

---KRHIKLINSVAEVINRDKEVDQYLKGCFHCKLXRFNRSSHRSSL*yqstnfnrwvpk
pxvxvt*nsl*mvv*slvlwmvqmlkspkklvkriclslvyvqvklklxvkk*qtkkstl
thvfkrysliln*vhlvhqmfsdqswtllifsdfylsiqdfplyldsqasvdelwkdqsa
wvkksiinsastyffssdramneyaeqiwdikpwkxnyfk*kilxk*

Frame C:
IIFININNFLLLIIIIVFFFFFXNFLFIXXNNNNNNNNNNNXXFX---

---kdisn*stllprlstvikrsintsrvvfianyxvsiaqvivpasdinqqistagyrs
lwx**heihyew*fnhwyfgwckc*nrrrswsreyvylwftyk*s*sxs*kndkqrsqh*
ptxsrgip*y*iryiwstrcfqtnlgls*slvist*afkishstltlklqlmnygkikvl
glknqsltvlqlisslliel*mnmlnkfgilnhgsxttlnrry*xn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK214 (SHK214Q) /CSM/SH/SHK2-A/SHK214Q.Seq.d/                       1292   0.0
SHG719 (SHG719Q) /CSM/SH/SHG7-A/SHG719Q.Seq.d/                       1231   0.0
CHQ714 (CHQ714Q) /CSM/CH/CHQ7-A/CHQ714Q.Seq.d/                       1223   0.0
SHK596 (SHK596Q) /CSM/SH/SHK5-D/SHK596Q.Seq.d/                       1219   0.0
CHL803 (CHL803Q) /CSM/CH/CHL8-A/CHL803Q.Seq.d/                       1217   0.0
CHH266 (CHH266Q) /CSM/CH/CHH2-C/CHH266Q.Seq.d/                       1215   0.0
SHL775 (SHL775Q) /CSM/SH/SHL7-D/SHL775Q.Seq.d/                       1213   0.0
CHL725 (CHL725Q) /CSM/CH/CHL7-B/CHL725Q.Seq.d/                       1213   0.0
SHF274 (SHF274Q) /CSM/SH/SHF2-D/SHF274Q.Seq.d/                       1209   0.0
SHJ739 (SHJ739Q) /CSM/SH/SHJ7-B/SHJ739Q.Seq.d/                       1207   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X62142|X62142.1 D. discoideum glpV gene for glycogen phosphorylase 1.   517   0.0    5
M77492|M77492.1 Dictyostelium discoideum glycoprotein phosphorylase 2 (glpD) gene, complete cds.    86   4e-27  5
AZ550934|AZ550934.1 ENTEY06TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68   2e-13  2
AY050311|AY050311.1 Entamoeba histolytica glycogen phosphorylase mRNA, complete cds.    68   1e-12  3
AZ693437|AZ693437.1 ENTKO14TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68   1e-11  2
BH149199|BH149199.1 ENTQG01TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    68   1e-11  2
AZ668559|AZ668559.1 ENTLD89TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    64   1e-10  2
CX589990|CX589990.1 TTE00019959 Amplicon Express - Conjugative Form Tetrahymena thermophila cDNA, mRNA sequence.    78   4e-10  1
AF542095|AF542095.1 Entamoeba histolytica glycogen phosphorylase-2 mRNA, partial cds.    70   9e-08  1
DN200044|DN200044.1 USDA-FP_138108 Citrus root weevil whole body adult Diaprepes abbreviatus cDNA clone WHDRWA009_E01 5', mRNA sequence.    36   1e-07  3
dna update 2006. 6. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK103367_1(AK103367|pid:none) Oryza sativa Japonica Group cDNA c...    92   4e-43
(Q9LKJ3) RecName: Full=Alpha-glucan phosphorylase, H isozyme;   ...    91   4e-43
AF326977_1(AF326977|pid:none) Oryza sativa alpha 1,4-glucan phos...    92   4e-43
EU857639_1(EU857639|pid:none) Zea mays starch phosphorylase 2 pr...    92   1e-42
X62142_1(X62142|pid:none) D. discoideum glpV gene for glycogen p...   175   1e-42
(Q00766) RecName: Full=Glycogen phosphorylase 1;          Short=...   175   1e-42
(Q9SD76) RecName: Full=Alpha-glucan phosphorylase, H isozyme;   ...    89   1e-41
CR382138_967(CR382138|pid:none) Debaryomyces hansenii strain CBS...   100   6e-41
CP001048_3930(CP001048|pid:none) Yersinia pseudotuberculosis PB1...    92   8e-41
AD0479(AD0479) glycogen phosphorylase (EC 2.4.1.1) [imported] - ...    92   8e-41
protein update 2009. 5.29
PSORT 

 psg: 1.04  gvh: 0.48  alm: 0.14  top: 0.63  tms: 0.07  mit: 0.30  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SHK214 is nuc
5' end seq. ID SHK214F
5' end seq. 
>SHK214F.Seq
TTATTATTTTTATTAATATTAATAATTTTCTTTTATTAATTATTATAATAGTTTTCTTTT
TTTTTTTTNATAATTTTTTATTTATAANGTTNAATAATAATAATAATAATAATAATAATA
ATAATATNCNTTTTNCCANNNNNNNNNN
Length of 5' end seq. 148
3' end seq. ID SHK214Z
3' end seq. 
>SHK214Z.Seq
NNNNNNNNNNTAAAAGACATATCAAATTAATCAACTCTGTTGCCGAGGTTATCAACCGTG
ATAAAGAGGTCGATCAATACCTCAAGGGTTGTTTTCATTGCAAATTACANCGTTTCAATC
GCTCAAGTCATCGTTCCAGCCTCTGATATCAATCAACAAATTTCAACCGCTGGGTACCGA
AGCCTNTGGTANTAGTAACATGAAATTCACTATGAATGGTAGTTTAATCATTGGTACTTT
GGATGGTGCAAATGTTGAAATCGCCGAAGAAGTTGGTCAAGAGAATATGTTTATCTTTGG
TTTACGTACAAGTGAAGTTGAAGCTGNTCGTGAAAAAATGACAAACAAAGAAGTCAACAT
TGACCCACGTNTTCAAGAGGTATTCCTTAATATTGAATTAGGTACATTTGGTCCACCAGA
TGTTTTCAGACCAATCTTGGACTCTCCTAATCTTTAGTGATTTCTACTTAAGCATTCAAG
ATTTCCCACTCTACCTTGACTCTCAAGCTTCAGTTGATGAATTATGGAAAGATCAAAGTG
CTTGGGTTAAAAAATCAATCATTAACAGTGCTTCAACTTATTTCTTCTCTTCTGATAGAG
CTATGAATGAATATGCTGAACAAATTTGGGATATTAAACCATGGAAGTNAAACTACTTTA
AATAGAAGATATTAANAAAATAA
Length of 3' end seq. 683
Connected seq. ID SHK214P
Connected seq. 
>SHK214P.Seq
TTATTATTTTTATTAATATTAATAATTTTCTTTTATTAATTATTATAATAGTTTTCTTTT
TTTTTTTTNATAATTTTTTATTTATAANGTTNAATAATAATAATAATAATAATAATAATA
ATAATATNCNTTTTNCCA----------TAAAAGACATATCAAATTAATCAACTCTGTTG
CCGAGGTTATCAACCGTGATAAAGAGGTCGATCAATACCTCAAGGGTTGTTTTCATTGCA
AATTACANCGTTTCAATCGCTCAAGTCATCGTTCCAGCCTCTGATATCAATCAACAAATT
TCAACCGCTGGGTACCGAAGCCTNTGGTANTAGTAACATGAAATTCACTATGAATGGTAG
TTTAATCATTGGTACTTTGGATGGTGCAAATGTTGAAATCGCCGAAGAAGTTGGTCAAGA
GAATATGTTTATCTTTGGTTTACGTACAAGTGAAGTTGAAGCTGNTCGTGAAAAAATGAC
AAACAAAGAAGTCAACATTGACCCACGTNTTCAAGAGGTATTCCTTAATATTGAATTAGG
TACATTTGGTCCACCAGATGTTTTCAGACCAATCTTGGACTCTCCTAATCTTTAGTGATT
TCTACTTAAGCATTCAAGATTTCCCACTCTACCTTGACTCTCAAGCTTCAGTTGATGAAT
TATGGAAAGATCAAAGTGCTTGGGTTAAAAAATCAATCATTAACAGTGCTTCAACTTATT
TCTTCTCTTCTGATAGAGCTATGAATGAATATGCTGAACAAATTTGGGATATTAAACCAT
GGAAGTNAAACTACTTTAAATAGAAGATATTAANAAAATAA
Length of connected seq. 811
Full length Seq ID -
Full length Seq. -
Length of full length seq. -