>SHK211 (SHK211Q) /CSM/SH/SHK2-A/SHK211Q.Seq.d/
CTGTTGGCCTACTGGNATATATTTTTTATCAATATATATTTAAAATATATAAAAAAATAA
AAAAATGCCTTTACGTTTAGATATTAAGAAAAAATTATCAACAAGATCAGATAGAGTTAA
ATCTGTTGATATTCACCCAACAGAACCATGGATTTTAGCAAGTTTATATGATGGTAATGT
TTATATTTGGAATTATGAAACTCAAAATATGGTAAAATCATTTGAAGTTTCACCAAATAA
TCCAGTTAGAACAGCAAGATTCATTGCAAAGAAACAATGGATAGTTACAGGTAGTGATGA
TACATATATTAGAGTTTATAATTATAATACAATGGAAAAGATAAAATCATTTGAAGCACA
TGCCGATTATATTAGATGTATTATAGTTCATCCAACATTACCATATATATTATCATCATC
GGATGATATGTTTATAAAGTTATGGGATTATGAAAAGGGATGGAGTAACACTCAAGTCTT
TGAAGGACATTCACATTACGTCATGTCGATTGCATGGAATCCAAAGGATACCAATCAATT
TGCAACCGCATCATTGGATAAAACTGTGAAAGTGTGGAGTATAAACTCACCACATCCTCA
CTTTACAXXXXXXXXXXATGGCACGTACCTATTTACCATCGATGGTACCTGGTATGGTTG
GGAAATGGAAAGAGAGTTTAAAGAGTACAAGCTCAAAAGCATCCGATGCACTTTCAAATC
CAATCGATTTCCCAAATTTATTCCCACAATATGATTTATCAATTAAAGCAGAGAAATATT
TCGAATCATTAAGATCTCAACCAAAATCATCTTTTGATTATTTAAAAAATGCTACTGCAC
AATCATTATCAAAGAAACAAAGAAACCTTTTAGAAGAGATTGAATCTGTTTCTCAACAAG
ATTATGATAAAGTTTTATCACAATTACAACATTTTAGTTTACATTCTGGTATTTCTGATA
TAACAACAACAACAACCAATACAAATAATAATACAAATAATACAAATAATAATAATACAG
CATCACTTTTAGATGATCTAGATTTTACTTCAACCACCACCACTACTACAACCACTACAA
ATACAAATACTACTCAAAAAGTTGCATCACCACTTTCATCACCATCACCAATTAGAAAGA
ATGATTCAATTGCTTCTCCACAAAAACAACAACAACAACAACAAGATATTAATATAACTG
CACCACCACTCATTGATCCAAGTCCAGTTGATCATGATTTAGTTTTAGAACCAACTCCAA
TTATTAATACTGTATCACCAACTAAAACTTCTCCAACTGCCGATCTCTCATTTTTAGATA
ATATACCAGTCGATCAGNANAAGAGG

cwptgiyflsiyi*ni*KNKKMPLRLDIKKKLSTRSDRVKSVDIHPTEPWILASLYDGNV
YIWNYETQNMVKSFEVSPNNPVRTARFIAKKQWIVTGSDDTYIRVYNYNTMEKIKSFEAH
ADYIRCIIVHPTLPYILSSSDDMFIKLWDYEKGWSNTQVFEGHSHYVMSIAWNPKDTNQF
ATASLDKTVKVWSINSPHPHFT---

---MARTYLPSMVPGMVGKWKESLKSTSSKASDALSNPIDFPNLFPQYDLSIKAEKYFES
LRSQPKSSFDYLKNATAQSLSKKQRNLLEEIESVSQQDYDKVLSQLQHFSLHSGISDITT
TTTNTNNNTNNTNNNNTASLLDDLDFTSTTTTTTTTTNTNTTQKVASPLSSPSPIRKNDS
IASPQKQQQQQQDINITAPPLIDPSPVDHDLVLEPTPIINTVSPTKTSPTADLSFLDNIP
VDQXKR

Frame A:
llaywxiffiniylkyikk*knaftfry*ekiinkir*s*ic*yspnrtmdfskfi*w*c
lylel*nskygkii*sftk*ss*nskihcketmdsyr***yiy*sl*l*yngkdkii*st
crly*myysssnitiyiiiig*yvykvmgl*kgme*hssl*rtftlrhvdcmeskgyqsi
cnriig*ncesveyklttssly---

---MARTYLPSMVPGMVGKWKESLKSTSSKASDALSNPIDFPNLFPQYDLSIKAEKYFES
LRSQPKSSFDYLKNATAQSLSKKQRNLLEEIESVSQQDYDKVLSQLQHFSLHSGISDITT
TTTNTNNNTNNTNNNNTASLLDDLDFTSTTTTTTTTTNTNTTQKVASPLSSPSPIRKNDS
IASPQKQQQQQQDINITAPPLIDPSPVDHDLVLEPTPIINTVSPTKTSPTADLSFLDNIP
VDQXKR

Frame B:
cwptgiyflsiyi*ni*KNKKMPLRLDIKKKLSTRSDRVKSVDIHPTEPWILASLYDGNV
YIWNYETQNMVKSFEVSPNNPVRTARFIAKKQWIVTGSDDTYIRVYNYNTMEKIKSFEAH
ADYIRCIIVHPTLPYILSSSDDMFIKLWDYEKGWSNTQVFEGHSHYVMSIAWNPKDTNQF
ATASLDKTVKVWSINSPHPHFT---

---whvpiyhrwylvwlgngkrv*rvqaqkhpmhfqiqsisqiyshnmiyqlkqrnisnh
*dlnqnhllii*kmllhnhyqrnketf*krlnlflnkimikfyhnynilvyilvfli*qq
qqpiqiiiqiiqiiiiqhhf*mi*illqpppllqplqiqillkklhhhfhhhhqlermiq
lllhknnnnnnkili*lhhhsliqvqlimi*f*nqlqllilyhqlkllqlpishf*iiyq
sixxr

Frame C:
vgllxyifyqyifkiykkikkclyv*ilrknyqqdqielnlliftqqnhgf*qvymmvmf
ifgimklkiw*nhlkfhqiiqleqqdslqrnng*lqvvmihilefiiiiqwkr*nhlkhm
piildvl*fiqhyhiyyhhrmicl*sygimkrdgvtlkslkdihitscrlhgiqripinl
qphhwikl*kcgv*thhiltl---

---gtylftidgtwygwemerefkeyklksirctfksnrfpkfipti*fin*sreifrii
kistkiif*lfkkcyctiiiketkkpfrrd*icfstrl**sfitittf*ftfwyf*ynnn
nnqyk**yk*yk***ysitfr*srfyfnhhhyynhykykyyskscittfititn*ke*fn
cfstkttttttry*yncttth*skss*s*fsfrtnsny*ycitn*nfsncrslifr*yts
rsxxe

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK211 (SHK211Q) /CSM/SH/SHK2-A/SHK211Q.Seq.d/                       1715   0.0
VHK584 (VHK584Q) /CSM/VH/VHK5-D/VHK584Q.Seq.d/                       1074   0.0
VHJ171 (VHJ171Q) /CSM/VH/VHJ1-C/VHJ171Q.Seq.d/                       1074   0.0
VHH424 (VHH424Q) /CSM/VH/VHH4-A/VHH424Q.Seq.d/                       1074   0.0
VHD704 (VHD704Q) /CSM/VH/VHD7-A/VHD704Q.Seq.d/                       1074   0.0
VHA788 (VHA788Q) /CSM/VH/VHA7-D/VHA788Q.Seq.d/                       1074   0.0
SHL384 (SHL384Q) /CSM/SH/SHL3-D/SHL384Q.Seq.d/                       1074   0.0
SHK466 (SHK466Q) /CSM/SH/SHK4-C/SHK466Q.Seq.d/                       1074   0.0
SHI247 (SHI247Q) /CSM/SH/SHI2-B/SHI247Q.Seq.d/                       1074   0.0
SHE433 (SHE433Q) /CSM/SH/SHE4-B/SHE433Q.Seq.d/                       1074   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB261051|AB261051.1 Entamoeba histolytica gene for beta prime-COP, complete cds.    66   4e-12  2
AZ684437|AZ684437.1 ENTLH77TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66   4e-12  2
CN942611|CN942611.1 010921AVBC069045PG (AVBC) Royal Gala young shoot Malus x domestica cDNA clone AVBC069045, mRNA sequence.    78   4e-12  2
CN914665|CN914665.1 030110ABNB001773HT (ABNB) Braeburn cultured fruit cells, boron exposed, phytoremediation Malus x domestica cDNA clone ABNB001773, mRNA sequence.    78   4e-12  2
CN939139|CN939139.1 010616AVBC004822HT (AVBC) Royal Gala young shoot Malus x domestica cDNA clone AVBC004822, mRNA sequence.    78   5e-12  2
AY266451|AY266451.1 Plasmodium falciparum isolate 3D7 COPI protein (Sec27) mRNA, complete cds.    84   7e-12  3
AL929355|AL929355.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 1/5.    84   1e-11  1
AP003487|AP003487.2 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, BAC clone:OSJNBa0007O20.    46   7e-11  3
AK111584|AK111584.1 Oryza sativa (japonica cultivar-group) cDNA clone:J013089O14, full insert sequence.    46   8e-11  3
CL809452|CL809452.1 OR_CBa0024E02.f OR_CBa Oryza rufipogon genomic clone OR_CBa0024E02 5', genomic survey sequence.    46   9e-11  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YD8) RecName: Full=Coatomer subunit beta'; AltName: Full=Bet...   383   e-105
(Q9C827) RecName: Full=Coatomer subunit beta'-2; AltName: Full=B...   295   3e-78
(Q9CAA0) RecName: Full=Coatomer subunit beta'-1; AltName: Full=B...   294   5e-78
(Q6H8D5) RecName: Full=Coatomer subunit beta'-2; AltName: Full=B...   293   9e-78
BT036985_1(BT036985|pid:none) Zea mays full-length cDNA clone ZM...   286   1e-75
AC011717_4(AC011717|pid:none) Arabidopsis thaliana chromosome 1 ...   280   8e-74
CP001574_321(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   278   2e-73
BC096500_1(BC096500|pid:none) Xenopus tropicalis hypothetical pr...   276   1e-72
AK304219_1(AK304219|pid:none) Homo sapiens cDNA FLJ61545 complet...   276   1e-72
(P35606) RecName: Full=Coatomer subunit beta'; AltName: Full=Bet...   276   1e-72

 psg: 0.43  gvh: 0.36  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SHK211 is cyt

>SHK211F.Seq
CTGTTGGCCTACTGGNATATATTTTTTATCAATATATATTTAAAATATATAAAAAAATAA
AAAAATGCCTTTACGTTTAGATATTAAGAAAAAATTATCAACAAGATCAGATAGAGTTAA
ATCTGTTGATATTCACCCAACAGAACCATGGATTTTAGCAAGTTTATATGATGGTAATGT
TTATATTTGGAATTATGAAACTCAAAATATGGTAAAATCATTTGAAGTTTCACCAAATAA
TCCAGTTAGAACAGCAAGATTCATTGCAAAGAAACAATGGATAGTTACAGGTAGTGATGA
TACATATATTAGAGTTTATAATTATAATACAATGGAAAAGATAAAATCATTTGAAGCACA
TGCCGATTATATTAGATGTATTATAGTTCATCCAACATTACCATATATATTATCATCATC
GGATGATATGTTTATAAAGTTATGGGATTATGAAAAGGGATGGAGTAACACTCAAGTCTT
TGAAGGACATTCACATTACGTCATGTCGATTGCATGGAATCCAAAGGATACCAATCAATT
TGCAACCGCATCATTGGATAAAACTGTGAAAGTGTGGAGTATAAACTCACCACATCCTCA
CTTTACANNNNNNNNNN

>SHK211Z.Seq
NNNNNNNNNNATGGCACGTACCTATTTACCATCGATGGTACCTGGTATGGTTGGGAAATG
GAAAGAGAGTTTAAAGAGTACAAGCTCAAAAGCATCCGATGCACTTTCAAATCCAATCGA
TTTCCCAAATTTATTCCCACAATATGATTTATCAATTAAAGCAGAGAAATATTTCGAATC
ATTAAGATCTCAACCAAAATCATCTTTTGATTATTTAAAAAATGCTACTGCACAATCATT
ATCAAAGAAACAAAGAAACCTTTTAGAAGAGATTGAATCTGTTTCTCAACAAGATTATGA
TAAAGTTTTATCACAATTACAACATTTTAGTTTACATTCTGGTATTTCTGATATAACAAC
AACAACAACCAATACAAATAATAATACAAATAATACAAATAATAATAATACAGCATCACT
TTTAGATGATCTAGATTTTACTTCAACCACCACCACTACTACAACCACTACAAATACAAA
TACTACTCAAAAAGTTGCATCACCACTTTCATCACCATCACCAATTAGAAAGAATGATTC
AATTGCTTCTCCACAAAAACAACAACAACAACAACAAGATATTAATATAACTGCACCACC
ACTCATTGATCCAAGTCCAGTTGATCATGATTTAGTTTTAGAACCAACTCCAATTATTAA
TACTGTATCACCAACTAAAACTTCTCCAACTGCCGATCTCTCATTTTTAGATAATATACC
AGTCGATCAGNANAAGAGG

>SHK211P.Seq
CTGTTGGCCTACTGGNATATATTTTTTATCAATATATATTTAAAATATATAAAAAAATAA
AAAAATGCCTTTACGTTTAGATATTAAGAAAAAATTATCAACAAGATCAGATAGAGTTAA
ATCTGTTGATATTCACCCAACAGAACCATGGATTTTAGCAAGTTTATATGATGGTAATGT
TTATATTTGGAATTATGAAACTCAAAATATGGTAAAATCATTTGAAGTTTCACCAAATAA
TCCAGTTAGAACAGCAAGATTCATTGCAAAGAAACAATGGATAGTTACAGGTAGTGATGA
TACATATATTAGAGTTTATAATTATAATACAATGGAAAAGATAAAATCATTTGAAGCACA
TGCCGATTATATTAGATGTATTATAGTTCATCCAACATTACCATATATATTATCATCATC
GGATGATATGTTTATAAAGTTATGGGATTATGAAAAGGGATGGAGTAACACTCAAGTCTT
TGAAGGACATTCACATTACGTCATGTCGATTGCATGGAATCCAAAGGATACCAATCAATT
TGCAACCGCATCATTGGATAAAACTGTGAAAGTGTGGAGTATAAACTCACCACATCCTCA
CTTTACA----------ATGGCACGTACCTATTTACCATCGATGGTACCTGGTATGGTTG
GGAAATGGAAAGAGAGTTTAAAGAGTACAAGCTCAAAAGCATCCGATGCACTTTCAAATC
CAATCGATTTCCCAAATTTATTCCCACAATATGATTTATCAATTAAAGCAGAGAAATATT
TCGAATCATTAAGATCTCAACCAAAATCATCTTTTGATTATTTAAAAAATGCTACTGCAC
AATCATTATCAAAGAAACAAAGAAACCTTTTAGAAGAGATTGAATCTGTTTCTCAACAAG
ATTATGATAAAGTTTTATCACAATTACAACATTTTAGTTTACATTCTGGTATTTCTGATA
TAACAACAACAACAACCAATACAAATAATAATACAAATAATACAAATAATAATAATACAG
CATCACTTTTAGATGATCTAGATTTTACTTCAACCACCACCACTACTACAACCACTACAA
ATACAAATACTACTCAAAAAGTTGCATCACCACTTTCATCACCATCACCAATTAGAAAGA
ATGATTCAATTGCTTCTCCACAAAAACAACAACAACAACAACAAGATATTAATATAACTG
CACCACCACTCATTGATCCAAGTCCAGTTGATCATGATTTAGTTTTAGAACCAACTCCAA
TTATTAATACTGTATCACCAACTAAAACTTCTCCAACTGCCGATCTCTCATTTTTAGATA
ATATACCAGTCGATCAGNANAAGAGG