>SHK209 (SHK209Q) /CSM/SH/SHK2-A/SHK209Q.Seq.d/
CTGTTGGCCTACTGGNGNATAGAGNGTTTGAATTACTCTTGGNTAAATTAGCAATTGAAG
NATCCAATAAAGAGAAAACAAATGCAAAGTTTACCAGATATATCAAAACGTACATTATTA
GAACAAAACAAAGCGGATATCTACAGAACAGTTAAACACAAAGGACCAATTGAATCATTT
GCAGATGTTAAATCAGTAATCAGTTCAATCAATACTAAACATGTTCCAATTGATATCATT
AAAACATTACGTATTCATTTAAACACTGCAGATAGAGATTGGATTCAATCATTTTTAGAT
AACGATGGTGTTCAACCAATTTTAAATATTTTAAAGAGACTCGAAAGAAATAAAAACCGA
AAAAGAAAAGAGCATTCAATTTTACAATGGGAATGTACAAGATGTATTGCAGCATTAATG
AAAATTAAAATTGGTATGGAATATATTGCATCATTTCCTCAAACTACAAATCTTATGGTA
TTATGTTTGGATACCCCATTAATTAAAGCTAAAACTTTGGTTTTAGAACTTTTAGCAGCA
ATTGCAGTTACTGATAGAGGTCATGGCGCCGTTTTAACTTCAATGATTTATCATAAAGAA
GTTAAAAAAGAAATXXXXXXXXXXGTTTCAAGGTGTTGGAAACTTTACCCAAAGATGAGA
GATGCTCGTAGTAACGATAACAAACTTTCACTCTTACATTTCTTGGCTAAAACTCTTCAA
GATCGTATTCCAGAGATTTGGAATATTGGTGCAGAGTTACCACATATCGAACATGCTTCA
GAGGTAAGTTTAAATAATATCATCAGTGATAGTAGTGAAATTAAACGTTCCATCGATTTG
ATTGAACGTGATTTCGTACCAATGATCAATGATCCATTGTTTGCTCATGATAAACATTGG
ATTCACAAAATCACCGAGTTCCAAAAGATCGCCAAAGTACAATATCAAAGAATTGAAAAG
GAAATCGATGAAATGAATAAAGCTTTCGAAGAAATCACCTCTTACTTTGGTGAACCAAAA
TCAACTCAACCCGATGTTTTCTTTTCGACAATTAATAACTTTTTAGAAGACCTTGAAAAA
GCCTACGGTGAATATCAAGCTATGATTAGAAAGGCTGAATTGGAAAATTCAAAAATGGAA
GATCCAGAAAAAGGTGGTCTTCAAGATCTTTCCTCTCAAATTAGATCTGGTCAATTATTC
AAAGATAGAAGAGTTGGTGATTCTGTCATTGCTCAAATGCAAAATGTTGATAGTTTAAGA
AAAAATTTAGAATCAACTTCAACCACAACTCCAAATACTCCACCAACTATTAAAATTGAA
TTACCTTCTCAATCAATTTTAAAACCATCAGGCCAATTAAAAAAATA

llaywxiexlnyswxn*QLKXPIKRKQMQSLPDISKRTLLEQNKADIYRTVKHKGPIESF
ADVKSVISSINTKHVPIDIIKTLRIHLNTADRDWIQSFLDNDGVQPILNILKRLERNKNR
KRKEHSILQWECTRCIAALMKIKIGMEYIASFPQTTNLMVLCLDTPLIKAKTLVLELLAA
IAVTDRGHGAVLTSMIYHKEVKKE---

---VSRCWKLYPKMRDARSNDNKLSLLHFLAKTLQDRIPEIWNIGAELPHIEHASEVSLN
NIISDSSEIKRSIDLIERDFVPMINDPLFAHDKHWIHKITEFQKIAKVQYQRIEKEIDEM
NKAFEEITSYFGEPKSTQPDVFFSTINNFLEDLEKAYGEYQAMIRKAELENSKMEDPEKG
GLQDLSSQIRSGQLFKDRRVGDSVIAQMQNVDSLRKNLESTSTTTPNTPPTIKIELPSQS
ILKPSGQLKK

Frame A:
llaywxiexlnyswxn*QLKXPIKRKQMQSLPDISKRTLLEQNKADIYRTVKHKGPIESF
ADVKSVISSINTKHVPIDIIKTLRIHLNTADRDWIQSFLDNDGVQPILNILKRLERNKNR
KRKEHSILQWECTRCIAALMKIKIGMEYIASFPQTTNLMVLCLDTPLIKAKTLVLELLAA
IAVTDRGHGAVLTSMIYHKEVKKE---

---VSRCWKLYPKMRDARSNDNKLSLLHFLAKTLQDRIPEIWNIGAELPHIEHASEVSLN
NIISDSSEIKRSIDLIERDFVPMINDPLFAHDKHWIHKITEFQKIAKVQYQRIEKEIDEM
NKAFEEITSYFGEPKSTQPDVFFSTINNFLEDLEKAYGEYQAMIRKAELENSKMEDPEKG
GLQDLSSQIRSGQLFKDRRVGDSVIAQMQNVDSLRKNLESTSTTTPNTPPTIKIELPSQS
ILKPSGQLKK

Frame B:
cwptgx*xv*itlg*isn*xiq*renkckvyqiyqnvhy*nktkristeqlntkdqlnhl
qmlnq*svqsilnmfqlislkhyvfi*tlqieigfnhf*itmvfnqf*if*rdskeikte
keksiqfyngnvqdvlqh**klklvwnilhhflklqilwyyvwiph*lklklwf*nf*qq
lqllievmapf*lq*fiikklkkk---

---fqgvgnftqr*emlvvtitnfhsyiswlklfkivfqrfgilvqsyhisnmlqr*v*i
issvivvklnvpsi*lnvisyq*smihcllminigftkspsskrspkynikelkrksmk*
iklskkspltlvnqnqlnpmfsfrqlitf*ktlkkptvnikl*lerlnwkiqkwkiqkkv
vfkifplkldlvnyskieelvilsllkckmliv*eki*nqlqpqlqilhqllklnyllnq
f*nhqan*kn

Frame C:
vgllxxrxfelllxklaiexsnkektnakftryiktyiirtkqsgylqns*tqrtn*iic
rc*isnqfnqy*tcsn*yh*nitysfkhcr*rldsiifr*rwcstnfkyfketrkk*kpk
kkrafnftmgmykmycsinen*nwygiyciissnyksygimfgypin*s*nfgfrtfssn
csy**rswrrfnfndls*rs*krn---

---fkvletlpkdercs**r*qtftltflg*nssrsysrdleywcrvttyrtcfrgkfk*
yhq****n*tfhrfd*t*frtndq*sivcs**tldsqnhrvpkdrqstiskn*kgnr*ne
*sfrrnhlllw*tkinstrcflfdn**lfrrp*kslr*issyd*kg*igkfkngrsrkrw
ssrsflsn*iwsiiqr*ksw*fchcsnakc**fkkkfrinfnhnskystny*n*itfsin
fktirpikki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK209 (SHK209Q) /CSM/SH/SHK2-A/SHK209Q.Seq.d/                       1901   0.0
VHD279 (VHD279Q) /CSM/VH/VHD2-D/VHD279Q.Seq.d/                       1431   0.0
AHN833 (AHN833Q) /CSM/AH/AHN8-B/AHN833Q.Seq.d/                       1431   0.0
AFK767 (AFK767Q) /CSM/AF/AFK7-C/AFK767Q.Seq.d/                       1431   0.0
SHB171 (SHB171Q) /CSM/SH/SHB1-C/SHB171Q.Seq.d/                       1372   0.0
AHI806 (AHI806Q) /CSM/AH/AHI8-A/AHI806Q.Seq.d/                       1372   0.0
AHI721 (AHI721Q) /CSM/AH/AHI7-A/AHI721Q.Seq.d/                       1372   0.0
SHA320 (SHA320Q) /CSM/SH/SHA3-A/SHA320Q.Seq.d/                       1292   0.0
AHN328 (AHN328Q) /CSM/AH/AHN3-B/AHN328Q.Seq.d/                       1255   0.0
CHL483 (CHL483Q) /CSM/CH/CHL4-D/CHL483Q.Seq.d/                       1251   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ748258|AJ748258.1 Dictyostelium discoideum mRNA for diaphanous-related formin dDia2  (drf2 gene).  1400   0.0    2
AC106872|AC106872.5 Homo sapiens BAC clone RP11-366M4 from 4, complete sequence.    36   0.022  2
AC053514|AC053514.4 Homo sapiens chromosome 12 clone RP11-408B11, WORKING DRAFT SEQUENCE, 7 unordered pieces.    48   0.55   1
CT573495|CT573495.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    48   0.55   1
BX547935|BX547935.12 Zebrafish DNA sequence from clone CH211-207C1 in linkage group 16.    48   0.55   1
AC126471|AC126471.4 Homo sapiens 12 BAC RP11-629O19 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    48   0.55   1
CV880073|CV880073.1 Mdst6017g16.y3 Mdst Malus x domestica cDNA clone Mdst6017g16 5', mRNA sequence.    46   2.2    1
CV656445|CV656445.1 Mdst6014g23.y1 Mdst Malus x domestica cDNA clone Mdst6014g23 5', mRNA sequence.    46   2.2    1
CO755649|CO755649.1 Mdfrt3044m02.y1 Mdfrt Malus x domestica cDNA clone Mdfrt3044m02 5', mRNA sequence.    46   2.2    1
CT657990|CT657990.1 Danio rerio EST, clone ZF_mu_148n23 5'.        46   2.2    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54N00) RecName: Full=Formin-H; AltName: Full=Diaphanous-relate...   444   e-123
BC007411_1(BC007411|pid:none) Homo sapiens diaphanous homolog 1 ...    90   2e-16
BC117257_1(BC117257|pid:none) Homo sapiens diaphanous homolog 1 ...    90   2e-16
AB209482_1(AB209482|pid:none) Homo sapiens mRNA for Diaphanous 1...    90   2e-16
DQ067453_1(DQ067453|pid:none) Homo sapiens diaphanous-1 (DIAPH1)...    90   2e-16
(O60610) RecName: Full=Protein diaphanous homolog 1; AltName: Fu...    90   2e-16
AY363395_1(AY363395|pid:none) Homo sapiens diaphanous 1 mRNA, co...    89   3e-16
(O08808) RecName: Full=Protein diaphanous homolog 1; AltName: Fu...    88   6e-16
AK156391_1(AK156391|pid:none) Mus musculus activated spleen cDNA...    88   6e-16
AB168412_1(AB168412|pid:none) Macaca fascicularis testis cDNA cl...    87   1e-15

 psg: 0.67  gvh: 0.34  alm: 0.31  top: 0.43  tms: 0.07  mit: 0.39  mip: 0.03
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 24.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	  4.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SHK209 is mit

>SHK209F.Seq
CTGTTGGCCTACTGGNGNATAGAGNGTTTGAATTACTCTTGGNTAAATTAGCAATTGAAG
NATCCAATAAAGAGAAAACAAATGCAAAGTTTACCAGATATATCAAAACGTACATTATTA
GAACAAAACAAAGCGGATATCTACAGAACAGTTAAACACAAAGGACCAATTGAATCATTT
GCAGATGTTAAATCAGTAATCAGTTCAATCAATACTAAACATGTTCCAATTGATATCATT
AAAACATTACGTATTCATTTAAACACTGCAGATAGAGATTGGATTCAATCATTTTTAGAT
AACGATGGTGTTCAACCAATTTTAAATATTTTAAAGAGACTCGAAAGAAATAAAAACCGA
AAAAGAAAAGAGCATTCAATTTTACAATGGGAATGTACAAGATGTATTGCAGCATTAATG
AAAATTAAAATTGGTATGGAATATATTGCATCATTTCCTCAAACTACAAATCTTATGGTA
TTATGTTTGGATACCCCATTAATTAAAGCTAAAACTTTGGTTTTAGAACTTTTAGCAGCA
ATTGCAGTTACTGATAGAGGTCATGGCGCCGTTTTAACTTCAATGATTTATCATAAAGAA
GTTAAAAAAGAAATNNNNNNNNNN

>SHK209Z.Seq
NNNNNNNNNNGTTTCAAGGTGTTGGAAACTTTACCCAAAGATGAGAGATGCTCGTAGTAA
CGATAACAAACTTTCACTCTTACATTTCTTGGCTAAAACTCTTCAAGATCGTATTCCAGA
GATTTGGAATATTGGTGCAGAGTTACCACATATCGAACATGCTTCAGAGGTAAGTTTAAA
TAATATCATCAGTGATAGTAGTGAAATTAAACGTTCCATCGATTTGATTGAACGTGATTT
CGTACCAATGATCAATGATCCATTGTTTGCTCATGATAAACATTGGATTCACAAAATCAC
CGAGTTCCAAAAGATCGCCAAAGTACAATATCAAAGAATTGAAAAGGAAATCGATGAAAT
GAATAAAGCTTTCGAAGAAATCACCTCTTACTTTGGTGAACCAAAATCAACTCAACCCGA
TGTTTTCTTTTCGACAATTAATAACTTTTTAGAAGACCTTGAAAAAGCCTACGGTGAATA
TCAAGCTATGATTAGAAAGGCTGAATTGGAAAATTCAAAAATGGAAGATCCAGAAAAAGG
TGGTCTTCAAGATCTTTCCTCTCAAATTAGATCTGGTCAATTATTCAAAGATAGAAGAGT
TGGTGATTCTGTCATTGCTCAAATGCAAAATGTTGATAGTTTAAGAAAAAATTTAGAATC
AACTTCAACCACAACTCCAAATACTCCACCAACTATTAAAATTGAATTACCTTCTCAATC
AATTTTAAAACCATCAGGCCAATTAAAAAAATA

>SHK209P.Seq
CTGTTGGCCTACTGGNGNATAGAGNGTTTGAATTACTCTTGGNTAAATTAGCAATTGAAG
NATCCAATAAAGAGAAAACAAATGCAAAGTTTACCAGATATATCAAAACGTACATTATTA
GAACAAAACAAAGCGGATATCTACAGAACAGTTAAACACAAAGGACCAATTGAATCATTT
GCAGATGTTAAATCAGTAATCAGTTCAATCAATACTAAACATGTTCCAATTGATATCATT
AAAACATTACGTATTCATTTAAACACTGCAGATAGAGATTGGATTCAATCATTTTTAGAT
AACGATGGTGTTCAACCAATTTTAAATATTTTAAAGAGACTCGAAAGAAATAAAAACCGA
AAAAGAAAAGAGCATTCAATTTTACAATGGGAATGTACAAGATGTATTGCAGCATTAATG
AAAATTAAAATTGGTATGGAATATATTGCATCATTTCCTCAAACTACAAATCTTATGGTA
TTATGTTTGGATACCCCATTAATTAAAGCTAAAACTTTGGTTTTAGAACTTTTAGCAGCA
ATTGCAGTTACTGATAGAGGTCATGGCGCCGTTTTAACTTCAATGATTTATCATAAAGAA
GTTAAAAAAGAAAT----------GTTTCAAGGTGTTGGAAACTTTACCCAAAGATGAGA
GATGCTCGTAGTAACGATAACAAACTTTCACTCTTACATTTCTTGGCTAAAACTCTTCAA
GATCGTATTCCAGAGATTTGGAATATTGGTGCAGAGTTACCACATATCGAACATGCTTCA
GAGGTAAGTTTAAATAATATCATCAGTGATAGTAGTGAAATTAAACGTTCCATCGATTTG
ATTGAACGTGATTTCGTACCAATGATCAATGATCCATTGTTTGCTCATGATAAACATTGG
ATTCACAAAATCACCGAGTTCCAAAAGATCGCCAAAGTACAATATCAAAGAATTGAAAAG
GAAATCGATGAAATGAATAAAGCTTTCGAAGAAATCACCTCTTACTTTGGTGAACCAAAA
TCAACTCAACCCGATGTTTTCTTTTCGACAATTAATAACTTTTTAGAAGACCTTGAAAAA
GCCTACGGTGAATATCAAGCTATGATTAGAAAGGCTGAATTGGAAAATTCAAAAATGGAA
GATCCAGAAAAAGGTGGTCTTCAAGATCTTTCCTCTCAAATTAGATCTGGTCAATTATTC
AAAGATAGAAGAGTTGGTGATTCTGTCATTGCTCAAATGCAAAATGTTGATAGTTTAAGA
AAAAATTTAGAATCAACTTCAACCACAACTCCAAATACTCCACCAACTATTAAAATTGAA
TTACCTTCTCAATCAATTTTAAAACCATCAGGCCAATTAAAAAAATA