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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHK132
Library SH
(Link to library)
Clone ID SHK132
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11115-1
Original site URL
Representative seq. ID SHK132P
(Link to Original site)
Representative DNA sequence 
>SHK132 (SHK132Q) /CSM/SH/SHK1-B/SHK132Q.Seq.d/
TTGGCCTACTGGNAAAGNAATGTATTATCATCATTACAAAGNAATTAATTCCGATGATTT
ACCATTTCAATCATATCTTTTGAAATGTTCAGGTCAAGAGATTATTCCAAAGTATTTCCA
AGATCATCCAGTTATTAATTTACAAACTACATTTGATAATACTGAACGTATCAATATTAG
AACATCGGAATTTCCAGAGAGATTGGGTACATTGGATCCAAGTCAAATCGAAGCATTTGA
ACATTGTTTGAAATCAGAGTTATCTTTAGTTCAAGGTCCACCAGGTACTGGTAAATCTTA
TATTGGTGTGAAATTATTCGAAGTGATTCATAGACATTTAAAAGAGAATTCAGCAAAGAA
TGCTCCAATATTGGTTTTATGTTATACCAACCATGCCTTGGATCAATTTTTGGATCATAT
CATTACAAACGTTACAAAGAAAGTAATTAGAGTCGGTTCAAGATCTCGTGATGAAAGAAT
GAAAGATTATAATATTCGTAATTTCATTCGTGCTGAAAAAAGACAGTTTGAATACTATAG
XXXXXXXXXXACTCATTCAGTTTGTAATACAGGTTGTTCAAAACTTTGTAACCCATGTAG
AGAGAAATGTAAACGTGGTTGTATTCATGTTGGTAAATGTAAAAACCTTTGTAAAGAACA
ATGTTCCATCAAGAATTGTGATCAAAGATGTACAAAAAAATTACCATGCAAACATCAATG
TATTGGTTTATGTGGTGAAAAATGTGTTTCAATTTGTAGAATATGTAATCCAGATTATTT
AGAACCAATCACTCGTATGAAGTTAAGTGAATTCGATGAAAATGATTGTTTCATTGAACT
TCATAAAGGCCATGTCTTCCTCGTTGACAACTTGGATACCTTTATGAATATTAAAGATGA
AAGTGGTAAGATAGTTCATAAAAAGTGTCCATCATGTAATATGATCATCTCTGCTAAAGA
TGCTCCAAGATATAAAGATATCATTAACAAAACTTGGAATCAAATAGATTCAATTAAAAG
AAAATTAATGGAACGTGTAACAAAAGAAGAAACTGATATGGTTGTAAAAGCAATGGGTGG
TGCTGCTGGACATTGGTTCGTTTGTGAAAATAAACATACATATTATATTGGTGAATGTGG
AGGAGCAATGCAAATAGGAAACTGTATTGAGTGTAAAGCTAAAGTTG
sequence update 2002.10.25
Translated Amino Acid sequence 
WPTGKXMYYHHYKXINSDDLPFQSYLLKCSGQEIIPKYFQDHPVINLQTTFDNTERINIR
TSEFPERLGTLDPSQIEAFEHCLKSELSLVQGPPGTGKSYIGVKLFEVIHRHLKENSAKN
APILVLCYTNHALDQFLDHIITNVTKKVIRVGSRSRDERMKDYNIRNFIRAEKRQFEYY-
--

---THSVCNTGCSKLCNPCREKCKRGCIHVGKCKNLCKEQCSIKNCDQRCTKKLPCKHQC
IGLCGEKCVSICRICNPDYLEPITRMKLSEFDENDCFIELHKGHVFLVDNLDTFMNIKDE
SGKIVHKKCPSCNMIISAKDAPRYKDIINKTWNQIDSIKRKLMERVTKEETDMVVKAMGG
AAGHWFVCENKHTYYIGECGGAMQIGNCIECKAKV
Translated Amino Acid sequence (All Frames) 
Frame A:
laywxxnvlsslqxn*fr*ftisiisfemfrsrdyskvfprsssy*ftnyi**y*tyqy*
nigisreigyigsksnrsi*tlfeirvifssrstryw*ilywceiirsds*tfkrefske
csnigfmlyqpclgsifgsyhykrykesn*srfkis**knerl*ys*fhsc*kktv*il*
---

---THSVCNTGCSKLCNPCREKCKRGCIHVGKCKNLCKEQCSIKNCDQRCTKKLPCKHQC
IGLCGEKCVSICRICNPDYLEPITRMKLSEFDENDCFIELHKGHVFLVDNLDTFMNIKDE
SGKIVHKKCPSCNMIISAKDAPRYKDIINKTWNQIDSIKRKLMERVTKEETDMVVKAMGG
AAGHWFVCENKHTYYIGECGGAMQIGNCIECKAKV

Frame B:
WPTGKXMYYHHYKXINSDDLPFQSYLLKCSGQEIIPKYFQDHPVINLQTTFDNTERINIR
TSEFPERLGTLDPSQIEAFEHCLKSELSLVQGPPGTGKSYIGVKLFEVIHRHLKENSAKN
APILVLCYTNHALDQFLDHIITNVTKKVIRVGSRSRDERMKDYNIRNFIRAEKRQFEYY-
--

---liqfviqvvqnfvthvernvnvvvfmlvnvktfvknnvpsrivikdvqknyhaninv
lvyvvknvfqfveyviqii*nqslv*s*vnsmkmivslnfikamssslttwipl*ilkmk
vvr*fiksvhhvi*ssllkmlqdikisltklgik*iqlken*wnv*qkkkliwl*kqwvv
lldigsfvkinihiilvnveeqck*etvlsvklkl

Frame C:
gllxkxciiiitkxlipmiyhfnhif*nvqvkrlfqsiskiiqlliyklhliilnvsile
hrnfqrdwvhwiqvkskhlniv*nqsyl*fkvhqvlvnlilv*nysk*fidi*kriqqrm
lqywfyviptmpwinfwiislqtlqrk*lesvqdlvmke*kiiifvisfvlkkdslnti-
--

---sfsl*yrlfktl*pm*rem*twlyscw*m*kpl*rtmfhqel*skmykkitmqtsmy
wfmw*kmcfnl*nm*srlfrtnhsyevk*ir*k*lfh*ts*rpclpr*qlgylyey*r*k
w*dss*kvsim*ydhlc*rcski*ryh*qnlesnrfn*kkingtcnkrrn*ygcksngwc
cwtlvrl*k*tyilyw*mwrsnanrkly*v*s*s
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK132 (SHK132Q) /CSM/SH/SHK1-B/SHK132Q.Seq.d/                       2234   0.0
VHA643 (VHA643Q) /CSM/VH/VHA6-B/VHA643Q.Seq.d/                       1221   0.0
SHJ862 (SHJ862Q) /CSM/SH/SHJ8-C/SHJ862Q.Seq.d/                       1221   0.0
SHE342 (SHE342Q) /CSM/SH/SHE3-B/SHE342Q.Seq.d/                       1221   0.0
SHD845 (SHD845Q) /CSM/SH/SHD8-B/SHD845Q.Seq.d/                       1221   0.0
VHO284 (VHO284Q) /CSM/VH/VHO2-D/VHO284Q.Seq.d/                       1213   0.0
AHO283 (AHO283Q) /CSM/AH/AHO2-D/AHO283Q.Seq.d/                       1213   0.0
AHC239 (AHC239Q) /CSM/AH/AHC2-B/AHC239Q.Seq.d/                       1213   0.0
AHA375 (AHA375Q) /CSM/AH/AHA3-D/AHA375Q.Seq.d/                       1146   0.0
AFD855 (AFD855Q) /CSM/AF/AFD8-C/AFD855Q.Seq.d/                       1130   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CO844087|CO844087.1 LM_GM5_001677 Locusta migratoria gregarious phase's midgut cDNAlibrary Locusta migratoria cDNA, mRNA sequence.    56   0.002  1
CO833765|CO833765.1 LM_GH5_001702 Locusta migratoria gregarious phase's head cDNA library Locusta migratoria cDNA, mRNA sequence.    56   0.002  1
AZ930665|AZ930665.1 474.dhz57c06.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz57c06.s1, DNA sequence.    56   0.002  1
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    54   0.002  11
DV990453|DV990453.1 GQ0251b.B7_F02 GQ025: Annual flush SHOOTS diurnal cycle- trees Picea glauca cDNA clone GenomeQuebec_Id:GQ0251b_F02 3', mRNA sequence.    50   0.12   1
CO244306|CO244306.1 WS0037.B21_L10 WS-X-A-3 Picea glauca cDNA clone WS0037_L10 3', mRNA sequence.    50   0.12   1
CR380959|CR380959.1 Candida glabrata strain CBS138 chromosome M complete sequence.    48   0.47   1
DY843211|DY843211.1 CTOZ9712.b1_P03.ab1 CTO(XYZ) dandelion Taraxacum officinale cDNA clone CTOZ9712, mRNA sequence.    46   1.9    1
DY621885|DY621885.1 ZM_BFb0286E10.f ZM_BFb Zea mays cDNA 3', mRNA sequence.    46   1.9    1
AC186663|AC186663.1 Zea mays chromosome 3 clone CH201-188C18; ZMMBBc0188C18, *** SEQUENCING IN PROGRESS ***, 21 unordered pieces.    46   1.9    1
dna update 2006. 5.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FJ009107_3(FJ009107|pid:none) Mucor circinelloides strain NRRL 3...   112   3e-23
AL591711_9(AL591711|pid:none) Mouse DNA sequence from clone RP23...   111   4e-23
BC025488_1(BC025488|pid:none) Mus musculus zinc finger, NFX1-typ...   111   4e-23
AX098230_1(AX098230|pid:none) Sequence 5 from Patent WO0118208.       107   6e-22
BC062347_1(BC062347|pid:none) Homo sapiens zinc finger, NFX1-typ...   107   6e-22
AL049766_4(AL049766|pid:none) Human DNA sequence from clone RP4-...   103   1e-20
AL049766_5(AL049766|pid:none) Human DNA sequence from clone RP4-...   103   1e-20
BC014041_1(BC014041|pid:none) Homo sapiens zinc finger, NFX1-typ...   103   1e-20
AK022641_1(AK022641|pid:none) Homo sapiens cDNA FLJ12579 fis, cl...   103   1e-20
AL049766_2(AL049766|pid:none) Human DNA sequence from clone RP4-...   103   1e-20
protein update 2009. 5.29
PSORT 

 psg: 0.61  gvh: 0.48  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: endoplasmic reticulum

>> prediction for SHK132 is cyt
5' end seq. ID SHK132F
5' end seq. 
>SHK132F.Seq
TTGGCCTACTGGNAAAGNAATGTATTATCATCATTACAAAGNAATTAATTCCGATGATTT
ACCATTTCAATCATATCTTTTGAAATGTTCAGGTCAAGAGATTATTCCAAAGTATTTCCA
AGATCATCCAGTTATTAATTTACAAACTACATTTGATAATACTGAACGTATCAATATTAG
AACATCGGAATTTCCAGAGAGATTGGGTACATTGGATCCAAGTCAAATCGAAGCATTTGA
ACATTGTTTGAAATCAGAGTTATCTTTAGTTCAAGGTCCACCAGGTACTGGTAAATCTTA
TATTGGTGTGAAATTATTCGAAGTGATTCATAGACATTTAAAAGAGAATTCAGCAAAGAA
TGCTCCAATATTGGTTTTATGTTATACCAACCATGCCTTGGATCAATTTTTGGATCATAT
CATTACAAACGTTACAAAGAAAGTAATTAGAGTCGGTTCAAGATCTCGTGATGAAAGAAT
GAAAGATTATAATATTCGTAATTTCATTCGTGCTGAAAAAAGACAGTTTGAATACTATAG
NNNNNNNNNN
Length of 5' end seq. 550
3' end seq. ID SHK132Z
3' end seq. 
>SHK132Z.Seq
NNNNNNNNNNACTCATTCAGTTTGTAATACAGGTTGTTCAAAACTTTGTAACCCATGTAG
AGAGAAATGTAAACGTGGTTGTATTCATGTTGGTAAATGTAAAAACCTTTGTAAAGAACA
ATGTTCCATCAAGAATTGTGATCAAAGATGTACAAAAAAATTACCATGCAAACATCAATG
TATTGGTTTATGTGGTGAAAAATGTGTTTCAATTTGTAGAATATGTAATCCAGATTATTT
AGAACCAATCACTCGTATGAAGTTAAGTGAATTCGATGAAAATGATTGTTTCATTGAACT
TCATAAAGGCCATGTCTTCCTCGTTGACAACTTGGATACCTTTATGAATATTAAAGATGA
AAGTGGTAAGATAGTTCATAAAAAGTGTCCATCATGTAATATGATCATCTCTGCTAAAGA
TGCTCCAAGATATAAAGATATCATTAACAAAACTTGGAATCAAATAGATTCAATTAAAAG
AAAATTAATGGAACGTGTAACAAAAGAAGAAACTGATATGGTTGTAAAAGCAATGGGTGG
TGCTGCTGGACATTGGTTCGTTTGTGAAAATAAACATACATATTATATTGGTGAATGTGG
AGGAGCAATGCAAATAGGAAACTGTATTGAGTGTAAAGCTAAAGTTG
Length of 3' end seq. 647
Connected seq. ID SHK132P
Connected seq. 
>SHK132P.Seq
TTGGCCTACTGGNAAAGNAATGTATTATCATCATTACAAAGNAATTAATTCCGATGATTT
ACCATTTCAATCATATCTTTTGAAATGTTCAGGTCAAGAGATTATTCCAAAGTATTTCCA
AGATCATCCAGTTATTAATTTACAAACTACATTTGATAATACTGAACGTATCAATATTAG
AACATCGGAATTTCCAGAGAGATTGGGTACATTGGATCCAAGTCAAATCGAAGCATTTGA
ACATTGTTTGAAATCAGAGTTATCTTTAGTTCAAGGTCCACCAGGTACTGGTAAATCTTA
TATTGGTGTGAAATTATTCGAAGTGATTCATAGACATTTAAAAGAGAATTCAGCAAAGAA
TGCTCCAATATTGGTTTTATGTTATACCAACCATGCCTTGGATCAATTTTTGGATCATAT
CATTACAAACGTTACAAAGAAAGTAATTAGAGTCGGTTCAAGATCTCGTGATGAAAGAAT
GAAAGATTATAATATTCGTAATTTCATTCGTGCTGAAAAAAGACAGTTTGAATACTATAG
----------ACTCATTCAGTTTGTAATACAGGTTGTTCAAAACTTTGTAACCCATGTAG
AGAGAAATGTAAACGTGGTTGTATTCATGTTGGTAAATGTAAAAACCTTTGTAAAGAACA
ATGTTCCATCAAGAATTGTGATCAAAGATGTACAAAAAAATTACCATGCAAACATCAATG
TATTGGTTTATGTGGTGAAAAATGTGTTTCAATTTGTAGAATATGTAATCCAGATTATTT
AGAACCAATCACTCGTATGAAGTTAAGTGAATTCGATGAAAATGATTGTTTCATTGAACT
TCATAAAGGCCATGTCTTCCTCGTTGACAACTTGGATACCTTTATGAATATTAAAGATGA
AAGTGGTAAGATAGTTCATAAAAAGTGTCCATCATGTAATATGATCATCTCTGCTAAAGA
TGCTCCAAGATATAAAGATATCATTAACAAAACTTGGAATCAAATAGATTCAATTAAAAG
AAAATTAATGGAACGTGTAACAAAAGAAGAAACTGATATGGTTGTAAAAGCAATGGGTGG
TGCTGCTGGACATTGGTTCGTTTGTGAAAATAAACATACATATTATATTGGTGAATGTGG
AGGAGCAATGCAAATAGGAAACTGTATTGAGTGTAAAGCTAAAGTTG
Length of connected seq. 1177
Full length Seq ID -
Full length Seq. -
Length of full length seq. -