>AFJ656 (AFJ656Q) /CSM/AF/AFJ6-C/AFJ656Q.Seq.d/
XXXXXXXXXXTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGT
AAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGAT
GAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGT
AAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAG
GTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTT
GAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTA
GGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAAT
GATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGAT
GACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAA
TTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGAGATAAT

---DMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGK
VSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLG
EYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEF
IQDNAAIEFKLPSSQTEI

Frame A:
---ryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*
sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir
*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*i
hsr*rch*iqitiisn*dn


Frame B:
---DMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGK
VSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLG
EYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEF
IQDNAAIEFKLPSSQTEI


Frame C:
---i*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslvvk
flhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*v
nt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllns
fkitlplnsnyhhlklr*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ656 (AFJ656Q) /CSM/AF/AFJ6-C/AFJ656Q.Seq.d/                       1168   0.0
AFI313 (AFI313Q) /CSM/AF/AFI3-A/AFI313Q.Seq.d/                       1168   0.0
AFO247 (AFO247Q) /CSM/AF/AFO2-B/AFO247Q.Seq.d/                       1162   0.0
AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1162   0.0
AFH196 (AFH196Q) /CSM/AF/AFH1-D/AFH196Q.Seq.d/                       1162   0.0
VFN727 (VFN727Q) /CSM/VF/VFN7-B/VFN727Q.Seq.d/                       1156   0.0
VFM432 (VFM432Q) /CSM/VF/VFM4-B/VFM432Q.Seq.d/                       1156   0.0
VFK816 (VFK816Q) /CSM/VF/VFK8-A/VFK816Q.Seq.d/                       1156   0.0
SFL246 (SFL246Q) /CSM/SF/SFL2-B/SFL246Q.Seq.d/                       1156   0.0
SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                       1156   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  2e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  3e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  3e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  1e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  2e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  8e-06  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  3e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  3e-05  1
BU791776|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  3e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   168   1e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   162   5e-39
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   162   8e-39
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   160   3e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   157   3e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   1e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   1e-35
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   151   1e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   3e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   3e-35

 psg: 0.84  gvh: 0.38  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ656 is cyt

>AFJ656Z.Seq
----------TCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGT
AAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGAT
GAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGT
AAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAG
GTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTT
GAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTA
GGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAAT
GATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGAT
GACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAA
TTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGAGATAAT