>SHJ732 (SHJ732Q) /CSM/SH/SHJ7-B/SHJ732Q.Seq.d/
CCAAACAAGAGCAAGCAAATCATCTGTTTCATCATTNGGAGGTGCTTCATTATCAATGGG
ATCAAATTCAGTAGCATGCGGAGGTTGTGGTGGTGGTTCAAGTGGTCCAAGAGGCCCAGG
TGGTGTTGCAGTTGGCGTANCAGTAGATGTTAATATTAATTTGGGTGTTGTTGNTGGNGG
TGTATTANGTCTTGTTGGTGGAATTTTAGGCGGAGGNGGCTCAAGTGGTTCATGTGGTTG
CCATTAAATTTATTAAATATATAACCTANCATAACATTACTACAAAATGTATCATTACAA
ATTNAATAAAATAATCTTAAACTTTTATTACTTTTTATGAAAAAAAAAAXXXXXXXXXX

QTRASKSSVSSXGGASLSMGSNSVACGGCGGGSSGPRGPGGVAVGVXVDVNINLGVVXGG
VLXLVGGILGGGGSSGSCGCH*iy*iynlx*hyykmyhykxnkiilnfyyfl*kk---

Frame A:
pnkskqiicfiixrcfiingikfssmrrlwwwfkwskrprwccswrxsrc*y*fgccxwx
cixscwwnfrrrxlkwfmwlplnllni*pxitllqnvslqix*nnlkllllfmkkk---


Frame B:
QTRASKSSVSSXGGASLSMGSNSVACGGCGGGSSGPRGPGGVAVGVXVDVNINLGVVXGG
VLXLVGGILGGGGSSGSCGCH*iy*iynlx*hyykmyhykxnkiilnfyyfl*kk---


Frame C:
kqeqanhlfhhxevlhyqwdqiq*haevvvvvqvvqeaqvvlqlaxq*mliliwvllxxv
yxvllvef*aexaqvvhvvaikfikyitxhnittkciitnxik*s*tfitfyekk---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHJ732 (SHJ732Q) /CSM/SH/SHJ7-B/SHJ732Q.Seq.d/                        624   e-178
SSM416 (SSM416Q) /CSM/SS/SSM4-A/SSM416Q.Seq.d/                        601   e-171
SSD448 (SSD448Q) /CSM/SS/SSD4-B/SSD448Q.Seq.d/                        601   e-171
SFI327 (SFI327Q) /CSM/SF/SFI3-B/SFI327Q.Seq.d/                        601   e-171
SSI578 (SSI578Q) /CSM/SS/SSI5-D/SSI578Q.Seq.d/                        599   e-170
SHC707 (SHC707Q) /CSM/SH/SHC7-A/SHC707Q.Seq.d/                        595   e-169
SHC681 (SHC681Q) /CSM/SH/SHC6-D/SHC681Q.Seq.d/                        579   e-164
SSL468 (SSL468Q) /CSM/SS/SSL4-C/SSL468Q.Seq.d/                        484   e-136
SSK638 (SSK638Q) /CSM/SS/SSK6-B/SSK638Q.Seq.d/                         78   1e-13
SSF563 (SSF563Q) /CSM/SS/SSF5-C/SSF563Q.Seq.d/                         78   1e-13

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CR022830|CR022830.1 Forward strand read from insert in 3'HPRT insertion targeting andchromosome engineering clone MHPP213d13.    38   0.010  2
CR078467|CR078467.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP10i04.    38   0.010  2
CR019573|CR019573.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP10i04.    38   0.012  2
AC169021|AC169021.3 Loxodonta africana clone VMRC15-436D21, WORKING DRAFT SEQUENCE, 5 unordered pieces.    48   0.14   1
AC129769|AC129769.4 Rattus norvegicus clone CH230-10O9, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    48   0.14   1
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    40   0.97   4
AZ277635|AZ277635.1 RPCI-23-168E3.TJ RPCI-23 Mus musculus genomic clone RPCI-23-168E3, DNA sequence.    38   1.4    2
CB962477|CB962477.1 AGENCOURT_13443301 NIH_MGC_187 Homo sapiens cDNA clone IMAGE:30320226 5', mRNA sequence.    34   1.4    2
AC126952|AC126952.5 Rattus norvegicus clone CH230-15I4, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    40   1.5    3
AC097273|AC097273.3 Genomic sequence for Mus musculus, clone RP23-118N13, complete sequence.    38   2.0    2

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 245,411,177

 psg: 0.88  gvh: 0.48  alm: 0.30  top: 0.57  tms: 0.07  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: Golgi
	  4.0 %: endoplasmic reticulum

>> prediction for SHJ732 is nuc

>SHJ732F.Seq
CCAAACAAGAGCAAGCAAATCATCTGTTTCATCATTNGGAGGTGCTTCATTATCAATGGG
ATCAAATTCAGTAGCATGCGGAGGTTGTGGTGGTGGTTCAAGTGGTCCAAGAGGCCCAGG
TGGTGTTGCAGTTGGCGTANCAGTAGATGTTAATATTAATTTGGGTGTTGTTGNTGGNGG
TGTATTANGTCTTGTTGGTGGAATTTTAGGCGGAGGNGGCTCAAGTGGTTCATGTGGTTG
CCATTAAATTTATTAAATATATAACCTANCATAACATTACTACAAAATGTATCATTACAA
ATTNAATAAAATAATCTTAAACTTTTATTACTTTTTATGAAAAAAAAAANNNNNNNNNN