>SHJ574 (SHJ574Q) /CSM/SH/SHJ5-D/SHJ574Q.Seq.d/
XXXXXXXXXXCCAACAAAGGTAACGATAATGTTGGAACAATTGGAGAAGATGGATAATCA
ACAATTACCATTCGTTGGTTTCTATGTTGCAAAATCACCAACACCAAATTTAGATAAGAA
ACTTTTCTCTTTAACTCCAAAAGATATCGTTGGAAATACAGAGTTTATCAATGATTATCA
AGTTCATTTCACTTCAATTATGGAACCTAATACTTCCTACATCATTATACCCTCAACTTT
TACACCAGGCATTGAGTATCCTTTCAATTTACGTGTTATTACCAATCAACATAATGTAAT
TCAAATATCACGTTTACCAGAATGGAGTACTCGTAAACTTTCTGGTGAATGGAGAGGTCA
ATCTTGTGGTGGTCGTTATTCAAATACTAGTTCAAGTTGGACACTAAATCCACGTTTTAG
ATTTAATTTACCAAGAGGTGGTCGTTTCACTATTATATTGGCTCAAGCTGAAAAACCATC
NNATAATGGTATTGGTTTCTATTATTTTAAAACTTTACAAGATGGTACACTAAGAGAATT
TGTTTGTAAAAGTGGTTTCATTTGTGGTAAANAAATAGNNTT

---TKVTIMLEQLEKMDNQQLPFVGFYVAKSPTPNLDKKLFSLTPKDIVGNTEFINDYQV
HFTSIMEPNTSYIIIPSTFTPGIEYPFNLRVITNQHNVIQISRLPEWSTRKLSGEWRGQS
CGGRYSNTSSSWTLNPRFRFNLPRGGRFTIILAQAEKPSXNGIGFYYFKTLQDGTLREFV
CKSGFICGKXIX

Frame A:
---qqr*r*cwnnwrrwiinnyhslvsmlqnhqhqi*irnfsl*lqkisleiqslsmiik
fislqlwnlilptslypqllhqalsilsiyvllpinim*fkyhvyqngvlvnflvngevn
lvvvviqilvqvgh*ihvldliyqevvvsllywlklknhximvlvsiilklykmvh*enl
fvkvvsfvvxk*x


Frame B:
---nkgndnvgtigedg*stitirwflcckitntkfr*etflfnskryrwkyrvyq*lss
sfhfnygt*yflhhytlnfytrh*vsfqftcyyqst*cnsnitftrmeys*tfw*mersi
lwwslfky*fkldtkstf*i*ftkrwsfhyyigss*ktix*wywfllf*nftrwytkric
l*kwfhlw*xnxx


Frame C:
---TKVTIMLEQLEKMDNQQLPFVGFYVAKSPTPNLDKKLFSLTPKDIVGNTEFINDYQV
HFTSIMEPNTSYIIIPSTFTPGIEYPFNLRVITNQHNVIQISRLPEWSTRKLSGEWRGQS
CGGRYSNTSSSWTLNPRFRFNLPRGGRFTIILAQAEKPSXNGIGFYYFKTLQDGTLREFV
CKSGFICGKXIX

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN536 (VHN536Q) /CSM/VH/VHN5-B/VHN536Q.Seq.d/                       1094   0.0
SHJ574 (SHJ574Q) /CSM/SH/SHJ5-D/SHJ574Q.Seq.d/                       1094   0.0
SHC342 (SHC342Q) /CSM/SH/SHC3-B/SHC342Q.Seq.d/                       1094   0.0
AHQ839 (AHQ839Q) /CSM/AH/AHQ8-B/AHQ839Q.Seq.d/                       1094   0.0
AHO860 (AHO860Q) /CSM/AH/AHO8-C/AHO860Q.Seq.d/                       1094   0.0
AHI542 (AHI542Q) /CSM/AH/AHI5-B/AHI542Q.Seq.d/                       1094   0.0
AHB644 (AHB644Q) /CSM/AH/AHB6-B/AHB644Q.Seq.d/                       1094   0.0
AHH266 (AHH266Q) /CSM/AH/AHH2-C/AHH266Q.Seq.d/                       1086   0.0
AHL696 (AHL696Q) /CSM/AH/AHL6-D/AHL696Q.Seq.d/                       1072   0.0
AHJ814 (AHJ814Q) /CSM/AH/AHJ8-A/AHJ814Q.Seq.d/                       1045   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AG086971|AG086971.1 Pan troglodytes DNA, clone: PTB-085H18.F.      46   0.91   1
AC164524|AC164524.5 Mus musculus  BAC RP23-438F8 (Roswell Park Cancer Institute (C57BL/6J Female) Mouse BAC Library) complete sequence.    34   1.8    2
AC156288|AC156288.5 Mus musculus 6 BAC RP24-246G10 (Roswell Park Cancer Institute (C57BL/6J Male) Mouse BAC Library) complete sequence.    34   1.8    2
U43375|U43375.1 Caenorhabditis elegans cosmid K09C4, complete sequence.    42   2.4    3
BB238588|BB238588.2 Mus musculus 3 days neonate thymus cDNA, RIKEN full-length enriched library, clone:A630072I12, 3' end partial sequence.    44   3.6    1
CV123066|CV123066.1 OSTF30005B10 pDONR201 Entry vector Caenorhabditis elegans cDNA, mRNA sequence.    44   3.6    1
CR186364|CR186364.1 Reverse strand read from insert in 5'HPRT insertion targeting and chromosome engineering clone MHPN46d24.    44   3.6    1
CR250482|CR250482.1 Reverse strand read from insert in 5'HPRT insertion targeting and chromosome engineering clone MHPN408l04.    44   3.6    1
CR115379|CR115379.1 Reverse strand read from insert in 5'HPRT insertion targeting and chromosome engineering clone MHPN111m08.    44   3.6    1
DU965366|DU965366.1 312103 Tomato MboI BAC Library Lycopersicon esculentum genomic clone SL_MboI0034J21 3, genomic survey sequence.    44   3.6    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9HC91) RecName: Full=Calpain-10;          EC=3.4.22.-; AltName...    51   3e-05
AK074807_1(AK074807|pid:none) Homo sapiens cDNA FLJ90326 fis, cl...    51   3e-05
(Q9ES66) RecName: Full=Calpain-10;          EC=3.4.22.-; AltName...    51   3e-05
AB113362_1(AB113362|pid:none) Rattus norvegicus Capn10 mRNA for ...    50   4e-05
AK088207_1(AK088207|pid:none) Mus musculus 2 days neonate thymus...    49   7e-05
(Q95LP4) RecName: Full=Calpain-10;          EC=3.4.22.-; AltName...    49   7e-05
(Q9ESK3) RecName: Full=Calpain-10;          EC=3.4.22.-; AltName...    49   7e-05
AF089089_1(AF089089|pid:none) Mus musculus calpain-like protease...    48   2e-04
BC108604_1(BC108604|pid:none) Xenopus laevis hypothetical protei...    47   4e-04
EU328307_1(EU328307|pid:none) Sus scrofa ussuricus calpain 10 is...    45   0.001

 psg: 0.75  gvh: 0.32  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SHJ574 is nuc

>SHJ574Z.Seq
NNNNNNNNNNCCAACAAAGGTAACGATAATGTTGGAACAATTGGAGAAGATGGATAATCA
ACAATTACCATTCGTTGGTTTCTATGTTGCAAAATCACCAACACCAAATTTAGATAAGAA
ACTTTTCTCTTTAACTCCAAAAGATATCGTTGGAAATACAGAGTTTATCAATGATTATCA
AGTTCATTTCACTTCAATTATGGAACCTAATACTTCCTACATCATTATACCCTCAACTTT
TACACCAGGCATTGAGTATCCTTTCAATTTACGTGTTATTACCAATCAACATAATGTAAT
TCAAATATCACGTTTACCAGAATGGAGTACTCGTAAACTTTCTGGTGAATGGAGAGGTCA
ATCTTGTGGTGGTCGTTATTCAAATACTAGTTCAAGTTGGACACTAAATCCACGTTTTAG
ATTTAATTTACCAAGAGGTGGTCGTTTCACTATTATATTGGCTCAAGCTGAAAAACCATC
NNATAATGGTATTGGTTTCTATTATTTTAAAACTTTACAAGATGGTACACTAAGAGAATT
TGTTTGTAAAAGTGGTTTCATTTGTGGTAAANAAATAGNNTT