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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ593
Library AF
(Link to library)
Clone ID AFJ593
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFJ593P
(Link to Original site)
Representative DNA sequence 
>AFJ593 (AFJ593Q) /CSM/AF/AFJ5-D/AFJ593Q.Seq.d/
GGATCATGANCATGATCATGANGGATCATTTGTTAAAATCTTANATTCTGATAATTTCCA
TAATTCAGTTTNTGAACATGACGTANCTNTTGTTNTGTTTTATGCCCCANGGGGCGGNCA
TTGTAAAACTTTAAAGCCNTTXXXXXXXXXXGANCCAGAATCAAATGATGCTCCAGTTAA
GGTTACCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCNAAGGATGTTTTGGT
TGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATT
AGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAA
TGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGA
TGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGA
ATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATAA
TGTTGAATCTAAAAAANANTCTTCGGCTAAACA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
DHXHDHXGSFVKILXSDNFHNSVXEHDVXXVXFYAPXGGHCKTLKP---

---XPESNDAPVKVTVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDV
ESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAA
IEFKLPSSQTDDNVESKKXSSAK
Translated Amino Acid sequence (All Frames) 
Frame A:
gs*x*s*xiic*nlxf**fp*fsx*t*rxxcxvlcpxgrxl*nfkax---

---XPESNDAPVKVTVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDV
ESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAA
IEFKLPSSQTDDNVESKKXSSAK

Frame B:
DHXHDHXGSFVKILXSDNFHNSVXEHDVXXVXFYAPXGGHCKTLKP---

---xqnqmmlqlrlplvplskswf*thxrmfwlnstlhgaviarillqstin*vnt*ktl
nlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlp
lnsnyhhlklmimlnlkxxlrln

Frame C:
imxmimxdhllksxiliisiiqfxnmtxllxcfmpxgaxivkl*sx---

---xrik*css*gyrwyhfqkvgfrltxgcfg*ilrsmvrslqescsnlr*ir*ilkrr*
iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch
*iqitiisn***c*i*kxxfg*t
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ593 (AFJ593Q) /CSM/AF/AFJ5-D/AFJ593Q.Seq.d/                        993   0.0
VSI423 (VSI423Q) /CSM/VS/VSI4-A/VSI423Q.Seq.d/                        797   0.0
VSG470 (VSG470Q) /CSM/VS/VSG4-C/VSG470Q.Seq.d/                        797   0.0
VFM432 (VFM432Q) /CSM/VF/VFM4-B/VFM432Q.Seq.d/                        797   0.0
SSK356 (SSK356Q) /CSM/SS/SSK3-C/SSK356Q.Seq.d/                        797   0.0
SLI330 (SLI330Q) /CSM/SL/SLI3-B/SLI330Q.Seq.d/                        797   0.0
SLH760 (SLH760Q) /CSM/SL/SLH7-C/SLH760Q.Seq.d/                        797   0.0
SLF318 (SLF318Q) /CSM/SL/SLF3-A/SLF318Q.Seq.d/                        797   0.0
SLA686 (SLA686Q) /CSM/SL/SLA6-D/SLA686Q.Seq.d/                        797   0.0
SFJ472 (SFJ472Q) /CSM/SF/SFJ4-C/SFJ472Q.Seq.d/                        797   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   509  e-140  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  2e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  3e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  3e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  1e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    62  8e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  8e-06  1
AJ243958|AJ243958.1 Kluyveromyces lactis partial YDR517W, PDI1 and YCL044C genes.    60  3e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  3e-05  1
BM595365|BM595365.1 17000687448324 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449712032 5', mRNA sequence.    58  1e-04  1
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   141   1e-32
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   135   6e-31
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   134   2e-30
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   132   8e-30
BC064163_1(BC064163|pid:none) Xenopus tropicalis hypothetical pr...   129   7e-29
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   128   1e-28
EF215324_1(EF215324|pid:none) Callithrix jacchus clone M22-40.3_...   127   2e-28
(P38657) RecName: Full=Protein disulfide-isomerase A3;          ...   127   2e-28
BC140529_1(BC140529|pid:none) Bos taurus protein disulfide isome...   127   2e-28
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   126   3e-28
protein update 2009. 4.24
PSORT 

 psg: 0.75  gvh: 0.46  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ593 is cyt
5' end seq. ID AFJ593F
5' end seq. 
>AFJ593F.Seq
GGATCATGANCATGATCATGANGGATCATTTGTTAAAATCTTANATTCTGATAATTTCCA
TAATTCAGTTTNTGAACATGACGTANCTNTTGTTNTGTTTTATGCCCCANGGGGCGGNCA
TTGTAAAACTTTAAAGCCNTT----------
Length of 5' end seq. 141
3' end seq. ID AFJ593Z
3' end seq. 
>AFJ593Z.Seq
----------GANCCAGAATCAAATGATGCTCCAGTTAAGGTTACCGTTGGTACCACTTT
CAAAAAGTTGGTTTTAGACTCACCNAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTG
CGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGT
TGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGA
AATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTC
TTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGC
CATTGAATTCAAATTACCATCATCTCAAACTGATGATAATGTTGAATCTAAAAAANANTC
TTCGGCTAAACA
Length of 3' end seq. 422
Connected seq. ID AFJ593P
Connected seq. 
>AFJ593P.Seq
GGATCATGANCATGATCATGANGGATCATTTGTTAAAATCTTANATTCTGATAATTTCCA
TAATTCAGTTTNTGAACATGACGTANCTNTTGTTNTGTTTTATGCCCCANGGGGCGGNCA
TTGTAAAACTTTAAAGCCNTT----------GANCCAGAATCAAATGATGCTCCAGTTAA
GGTTACCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCNAAGGATGTTTTGGT
TGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATT
AGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAA
TGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGA
TGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGA
ATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATAA
TGTTGAATCTAAAAAANANTCTTCGGCTAAACA
Length of connected seq. 563
Full length Seq ID -
Full length Seq. -
Length of full length seq. -