>AFJ582 (AFJ582Q) /CSM/AF/AFJ5-D/AFJ582Q.Seq.d/
TATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTT
ATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAA
AGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGA
TGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAA
AGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTT
CAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGA
TTGNGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCANCATTCGATGG
NGAATCTGAATATTCCATTATGTTTGGTCCAXXXXXXXXXX

iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
XGGSYIKLLPSKLDQSXFDGESEYSIMFG---

Frame A:
yl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rr
w*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*
lxwfihqvitiqirsixirwxi*ifhyvws---


Frame B:
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
XGGSYIKLLPSKLDQSXFDGESEYSIMFG---


Frame C:
fiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkm
vnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkli
xvvhtssyyhpn*inxhsmxnlniplclv---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO703 (VFO703Q) /CSM/VF/VFO7-A/VFO703Q.Seq.d/                        876   0.0
VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        876   0.0
VFM885 (VFM885Q) /CSM/VF/VFM8-D/VFM885Q.Seq.d/                        876   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        876   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        876   0.0
VFL283 (VFL283Q) /CSM/VF/VFL2-D/VFL283Q.Seq.d/                        876   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        876   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        876   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        876   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        876   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   868  0.0  1
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    86  6e-18  2
AL270631|AL270631.1 Tetraodon nigroviridis genome survey sequence PUC-Ori end of clone  075I07 of library G from Tetraodon nigroviridis.    44  0.093  2
BX548066|BX548066.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-226F1.    48  0.097  1
AF543476|AF543476.1 Uncultured bacterium plasmid pAK112 YjhP (yjhP) gene, partial cds; putative transmembrane protein and probable integral membrane protein genes, complete cds; and unknown gene.    48  0.097  1
AC124971|AC124971.14 Medicago truncatula clone mth2-22h8, complete sequence.    36  0.21  6
AZ529588|AZ529588.1 ENTCG34TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40  0.32  2
AC122073|AC122073.3 Rattus norvegicus clone CH230-439L24, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    46  0.38  1
AL483276|AL483276.1 T. brucei sheared genomic DNA clone 257b04, forward sequence.    46  0.38  1
AL489451|AL489451.1 T. brucei sheared genomic DNA clone 306e10, reverse sequence.    46  0.38  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   244   8e-64
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   157   1e-37
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   156   2e-37
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   155   4e-37
EF084103_1(EF084103|pid:none) Picea sitchensis clone WS0274_F04 ...   155   4e-37
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   155   5e-37
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   155   5e-37
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   155   5e-37
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   155   5e-37
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   155   5e-37

 psg: 1.01  gvh: 0.69  alm: 0.60  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFJ582 is exc

>AFJ582F.Seq
TATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTT
ATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAA
AGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGA
TGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAA
AGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTT
CAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGA
TTGNGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCANCATTCGATGG
NGAATCTGAATATTCCATTATGTTTGGTCCA----------