>SHI546 (SHI546Q) /CSM/SH/SHI5-B/SHI546Q.Seq.d/
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGATXXXXXXXXXX

VNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHL
KCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDS
IADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEY
QCKGWISGFTKCDWKGDSIER---

Frame A:
VNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHL
KCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDS
IADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEY
QCKGWISGFTKCDWKGDSIER---


Frame B:
liakekwqqkihllmrlnmqrvidqhvqpvkevlikkqfvlvikqnqntlmewmyhgii*
nvnvhkyhhlqi*ftgntfvgktnyqlkqlifhlksmiqnqhqrykerni*rhygklkiv
lpit*rdqqlktsfniikvmliryhqfiyfilsligc*kvvqvdvqlvrisiyfsmvsni
nvkvgfqvsqnvigkviqlk---


Frame C:
**qkkngnkkyisl*d*icke**inmfnlskry**rsssywl*nkiktl*wngcimasfk
m*mststiiyrfnslgipslgrpiin*nnlffi*kv*skisirgtkreifkgimgs*r*y
cr*legtsn*khhsi**rlc**gitssftsysl*ldvkrssr*msnl*efrytfqwyris
m*rldfrfhkm*ler*fn*k---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHM584 (VHM584Q) /CSM/VH/VHM5-D/VHM584Q.Seq.d/                       1197   0.0
VHH215 (VHH215Q) /CSM/VH/VHH2-A/VHH215Q.Seq.d/                       1197   0.0
VFM520 (VFM520Q) /CSM/VF/VFM5-A/VFM520Q.Seq.d/                       1197   0.0
SHJ436 (SHJ436Q) /CSM/SH/SHJ4-B/SHJ436Q.Seq.d/                       1197   0.0
SHI546 (SHI546Q) /CSM/SH/SHI5-B/SHI546Q.Seq.d/                       1197   0.0
SHI485 (SHI485Q) /CSM/SH/SHI4-D/SHI485Q.Seq.d/                       1197   0.0
SHG666 (SHG666Q) /CSM/SH/SHG6-C/SHG666Q.Seq.d/                       1197   0.0
SHF645 (SHF645Q) /CSM/SH/SHF6-B/SHF645Q.Seq.d/                       1197   0.0
SHF241 (SHF241Q) /CSM/SH/SHF2-B/SHF241Q.Seq.d/                       1197   0.0
CHQ835 (CHQ835Q) /CSM/CH/CHQ8-B/CHQ835Q.Seq.d/                       1197   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).  1197   0.0    1
AC025807|AC025807.2 Homo sapiens clone RP11-657G2, WORKING DRAFT SEQUENCE, 15 unordered pieces.    50   0.061  1
AM113503|AM113503.1 Streptococcus sp. 2410 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 2410.    50   0.061  1
AM113502|AM113502.1 Streptococcus sp. 3137 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 3137.    50   0.061  1
AM113501|AM113501.1 Streptococcus sp. 1956 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1956.    50   0.061  1
AM113500|AM113500.1 Streptococcus sp. 1504 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1504.    50   0.061  1
AM113497|AM113497.1 Streptococcus sp. 578 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 578.    50   0.061  1
AM113496|AM113496.1 Streptococcus pseudopneumoniae lytA gene for  N-acetylmuramoyl-L-alanine amidase, strain CCUG 48465.    50   0.061  1
AM113495|AM113495.1 Streptococcus pseudopneumoniae lytA gene for  N-acetylmuramoyl-L-alanine amidase, type strain CCUG 49455.    50   0.061  1
AC107307|AC107307.3 Homo sapiens 3 BAC RP11-657G2 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    50   0.061  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9ZP54) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...    75   2e-12
(P26446) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...    72   2e-11
(Q9ZSV1) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...    68   2e-10
AM427768_1(AM427768|pid:none) Vitis vinifera contig VV78X161143....    68   2e-10
(P18493) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...    68   2e-10
(Q7EYV7) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...    68   2e-10
GM894209_1(GM894209|pid:none) Sequence 10 from Patent EP1997899.       67   3e-10
AJ222589_1(AJ222589|pid:none) Zea mays mRNA for poly(ADP-ribose)...    67   3e-10
(P35875) RecName: Full=Poly [ADP-ribose] polymerase;          Sh...    66   6e-10
(Q11208) RecName: Full=Poly [ADP-ribose] polymerase;          Sh...    62   2e-08

 psg: 0.58  gvh: 0.23  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 32.0 %: nuclear
	 20.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for SHI546 is mit

>SHI546F.Seq
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGATNNNNNNNNNN