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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ562
Library AF
(Link to library)
Clone ID AFJ562
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16513-1
Original site URL
Representative seq. ID AFJ562P
(Link to Original site)
Representative DNA sequence 
>AFJ562 (AFJ562Q) /CSM/AF/AFJ5-C/AFJ562Q.Seq.d/
TTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTG
GCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTT
GATNCAGTTGTTGATGGTTCAAAAACTGTNTTTGTAAAATTNTATGCNCCATGGNGNGGC
CATTGTAAAAAACTTGCNCCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCC
AACAAAGTTGTAATTGCCAAAGTTGATTGNGACCAAGCTGACAACAAAGCTNTNTGTAGT
AAATATGATGTTTCTGGTTATCCAACCCTCAAAATNTTTGACAAATCAACCACTGCCAAA
GATTACAACGGNGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACNCCAAA
ACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTNTATGCACCATGGNGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGNGTCACTGGTTTCCCAACACTCAAAXXXXXXXXXXAGGTTCATACTACGTCA
AAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCTCGTA
TCACCAAATTAGTCTCTGGTTCNATGTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAAC
TCAATATTTTAGAATC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDXVVDGSKTVFVKXYAPWXG
HCKKLAPDFEILADTFAPVSNKVVIAKVDXDQADNKAXCSKYDVSGYPTLKXFDKSTTAK
DYNGARSVDELLTYINNHXKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVLVEXYAPWX
GHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLK---

---GSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEFAKKLNILE
Translated Amino Acid sequence (All Frames) 
Frame A:
LLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDXVVDGSKTVFVKXYAPWXG
HCKKLAPDFEILADTFAPVSNKVVIAKVDXDQADNKAXCSKYDVSGYPTLKXFDKSTTAK
DYNGARSVDELLTYINNHXKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVLVEXYAPWX
GHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLK---

---rfilrqshenhc*kinrfcnh*nrsyhqislwfxvwkes**icqetqyfri

Frame B:
y**yihke*kyyyllh*lhwhllpyaqqkemllfyhqttlxqllmvqklxl*nxmxhgxa
ivknlxqilksllthshqfptkl*lpklixtklttklxvvnmmflviqpskxltnqplpk
ittxldqlmny*lisiitpkptlk*rkhhqmlliyhhqiliqlf*inqrmf*lnxmhhgv
viaks*cqimkslvtptpmkkmlsspkliamllitkpsapnmxslvsqhs---

---GSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEFAKKLNILE

Frame C:
inntstkneniiicyincigicclmlsrrkcccfitrql*xsc*wfkncxckixcxmxxp
l*ktcxrf*npc*hirtsfqqscncqs*lxps*qqsxx**i*cfwlsnpqnx*qinhcqr
lqrx*is**iinlyq*sxqnqr*skestikcc*fitikf*fscfr*ikecfs*ixctmxw
slqkvnarl*npw*hlrq*krcchrqn*lrcc**qshllqiwxhwfpntq---

---vhttsks*kpllknqsil*plkslvspn*slvxclerklmnlprnsif*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO241 (VFO241Q) /CSM/VF/VFO2-B/VFO241Q.Seq.d/                       1279   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                       1279   0.0
VFM308 (VFM308Q) /CSM/VF/VFM3-A/VFM308Q.Seq.d/                       1279   0.0
VFL370 (VFL370Q) /CSM/VF/VFL3-C/VFL370Q.Seq.d/                       1279   0.0
VFL332 (VFL332Q) /CSM/VF/VFL3-B/VFL332Q.Seq.d/                       1279   0.0
VFI769 (VFI769Q) /CSM/VF/VFI7-C/VFI769Q.Seq.d/                       1279   0.0
VFG408 (VFG408Q) /CSM/VF/VFG4-A/VFG408Q.Seq.d/                       1279   0.0
VFF762 (VFF762Q) /CSM/VF/VFF7-C/VFF762Q.Seq.d/                       1279   0.0
VFF615 (VFF615Q) /CSM/VF/VFF6-A/VFF615Q.Seq.d/                       1279   0.0
VFF572 (VFF572Q) /CSM/VF/VFF5-C/VFF572Q.Seq.d/                       1279   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  1273  0.0  3
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   626  0.0  8
BX252003|BX252003.1 Pinus pinaster xylem EST, clone PP058D07.     46  5e-13  4
BQ695627|BQ695627.1 NXPV_030_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_030_G06 5', mRNA sequence.    54  9e-13  3
AU299541|AU299541.1 Cryptomeria japonica mRNA, clone: CM1065, 5' end, expressed in male cone.    60  5e-10  2
CB090389|CB090389.1 gy74e10.g1 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone gy74e10, mRNA sequence.    68  2e-09  2
AW290132|AW290132.1 NXNV012E07F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone NXNV012E07 5', mRNA sequence.    54  4e-09  2
CD026805|CD026805.1 NXNV012E07 Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone NXNV012E07 5' similar to Arabidopsis thaliana sequence At2g47470 putative protein disulfide-isomerase see http://mips.gsf.de/proj/thal/db/index.html, mRNA sequence.    54  4e-09  2
CB091549|CB091549.1 he93f09.g1 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone he93f09, mRNA sequence.    68  2e-07  1
BQ118738|BQ118738.1 EST604327 mixed potato tissues Solanum tuberosum cDNA clone STMEE06 3' end, mRNA sequence.    62  3e-07  2
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   405   e-111
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   405   e-111
AY730725_1(AY730725|pid:none) Entamoeba histolytica protein disu...   198   2e-49
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   198   2e-49
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   190   4e-47
(O22263) RecName: Full=Probable protein disulfide-isomerase A6; ...   189   7e-47
AK316899_1(AK316899|pid:none) Arabidopsis thaliana AT2G47470 mRN...   189   7e-47
AB182629_1(AB182629|pid:none) Glycine max Gm pdis-1a mRNA for pr...   189   7e-47
AY570240_1(AY570240|pid:none) Brassica napus var. napus clone BN...   189   9e-47
T03644(T03644) probable protein disulfide-isomerase (EC 5.3.4.1)...   186   1e-45
protein update 2009. 4.24
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.45  top: 0.10  tms: 0.00  mit: 0.34  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for AFJ562 is exc
5' end seq. ID AFJ562F
5' end seq. 
>AFJ562F.Seq
TTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTG
GCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTT
GATNCAGTTGTTGATGGTTCAAAAACTGTNTTTGTAAAATTNTATGCNCCATGGNGNGGC
CATTGTAAAAAACTTGCNCCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCC
AACAAAGTTGTAATTGCCAAAGTTGATTGNGACCAAGCTGACAACAAAGCTNTNTGTAGT
AAATATGATGTTTCTGGTTATCCAACCCTCAAAATNTTTGACAAATCAACCACTGCCAAA
GATTACAACGGNGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACNCCAAA
ACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTNTATGCACCATGGNGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGNGTCACTGGTTTCCCAACACTCAAA----------
Length of 5' end seq. 693
3' end seq. ID AFJ562Z
3' end seq. 
>AFJ562Z.Seq
----------AGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCG
ATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCNATGTCTGGAA
AGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATC
Length of 3' end seq. 153
Connected seq. ID AFJ562P
Connected seq. 
>AFJ562P.Seq
TTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTG
GCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTT
GATNCAGTTGTTGATGGTTCAAAAACTGTNTTTGTAAAATTNTATGCNCCATGGNGNGGC
CATTGTAAAAAACTTGCNCCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCC
AACAAAGTTGTAATTGCCAAAGTTGATTGNGACCAAGCTGACAACAAAGCTNTNTGTAGT
AAATATGATGTTTCTGGTTATCCAACCCTCAAAATNTTTGACAAATCAACCACTGCCAAA
GATTACAACGGNGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACNCCAAA
ACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTNTATGCACCATGGNGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGNGTCACTGGTTTCCCAACACTCAAA----------AGGTTCATACTACGTCA
AAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCTCGTA
TCACCAAATTAGTCTCTGGTTCNATGTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAAC
TCAATATTTTAGAATC
Length of connected seq. 846
Full length Seq ID -
Full length Seq. -
Length of full length seq. -