>SHI270 (SHI270Q) /CSM/SH/SHI2-C/SHI270Q.Seq.d/
ATTAATATCAAGTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATGATCA
ACGTATTTTGGCATTAAAAGGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGA
TGAAGGTGAACCAGGATTTGGAAATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAA
GGGAAATAAATATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATGGATT
AACTCAACGTACAACTTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAAC
CTATGAAAAGACATTGGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGC
TGATAGTTTGGCAGCAGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGAT
TAAACAAGTTGGTGTTTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTT
GGGTTATGTTGAGCAAACTCAACCACCAACTCAACCACCTACTCAACCACCTACTCAACC
ACCAACACCTCCACCATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAA
TGGTAATGGAATTTTTGATGGAAGXXXXXXXXXX

LISSIILNTVLNISSQVYDQRILALKGKCYLDSNKNALYDEGEPGFGNVEISLGNATDYK
GNKYPIRYSSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSA
DSLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQP
PTPPPFTGSAVAGLLFTDINGNGIFDG---

Frame A:
inikynfkycikyfitsi*styfgikrkmlfrfk*kciv**r*triwkc*nffrkcn*l*
gk*isntlfic*wyiyi*winstynl*iyiyqskrlll*nl*kdiglstrctmyssngfs
**fgsrsfsckylcl*hsid*tswcfirliytys*n*c*fglc*ansttnsttysttyst
tntstiyw*crrwfiiyryqw*wnf*w---


Frame B:
LISSIILNTVLNISSQVYDQRILALKGKCYLDSNKNALYDEGEPGFGNVEISLGNATDYK
GNKYPIRYSSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSA
DSLAADLLVANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQP
PTPPPFTGSAVAGLLFTDINGNGIFDG---


Frame C:
*yqv*f*ily*ifhhkyminvfwh*kenvi*iqikmhcmmkvnqdlemlkfl*emqliir
einiqyvihllmvhlymmd*lnvqlmnlhlpiqkiitlkpmkrhwlvhqvyhvfvqrfql
ivwqqif*lqiplpitfh*lnklvfhqahlylqlelvliwvmlsklnhqlnhllnhllnh
qhlhhllvvpslvyylpismvmeflme---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHI270 (SHI270Q) /CSM/SH/SHI2-C/SHI270Q.Seq.d/                       1237   0.0
SHL883 (SHL883Q) /CSM/SH/SHL8-D/SHL883Q.Seq.d/                       1229   0.0
SHL845 (SHL845Q) /CSM/SH/SHL8-B/SHL845Q.Seq.d/                       1229   0.0
SHL696 (SHL696Q) /CSM/SH/SHL6-D/SHL696Q.Seq.d/                       1229   0.0
SHL313 (SHL313Q) /CSM/SH/SHL3-A/SHL313Q.Seq.d/                       1229   0.0
SHK516 (SHK516Q) /CSM/SH/SHK5-A/SHK516Q.Seq.d/                       1229   0.0
SHJ418 (SHJ418Q) /CSM/SH/SHJ4-A/SHJ418Q.Seq.d/                       1229   0.0
SHJ245 (SHJ245Q) /CSM/SH/SHJ2-B/SHJ245Q.Seq.d/                       1229   0.0
SHI817 (SHI817Q) /CSM/SH/SHI8-A/SHI817Q.Seq.d/                       1229   0.0
SHI661 (SHI661Q) /CSM/SH/SHI6-C/SHI661Q.Seq.d/                       1229   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   2e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   2e-05  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   2e-05  1
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   2e-05  3
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   3e-04  1
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   3e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   3e-04  1
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   3e-04  3
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.001  3
DT775356|DT775356.1 124719991 TH1 Tribolium castaneum cDNA clone 3C7 3', mRNA sequence.    54   0.004  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000284_2387(CP000284|pid:none) Methylobacillus flagellatus KT,...    49   9e-05
CP000686_1034(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    46   0.001
CP001337_286(CP001337|pid:none) Chloroflexus aggregans DSM 9485,...    42   0.018
BX294149_114(BX294149|pid:none) Rhodopirellula baltica SH 1 comp...    42   0.018
CP000830_3576(CP000830|pid:none) Dinoroseobacter shibae DFL 12, ...    40   0.040
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    38   0.26
CP000804_904(CP000804|pid:none) Roseiflexus castenholzii DSM 139...    37   0.58
CP000909_3116(CP000909|pid:none) Chloroflexus aurantiacus J-10-f...    37   0.58
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    36   0.76
AM263198_431(AM263198|pid:none) Listeria welshimeri serovar 6b s...    35   1.3

 psg: 0.87  gvh: 0.46  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SHI270 is nuc

>SHI270F.Seq
ATTAATATCAAGTATAATTTTAAATACTGTATTAAATATTTCATCACAAGTATATGATCA
ACGTATTTTGGCATTAAAAGGAAAATGTTATTTAGATTCAAATAAAAATGCATTGTATGA
TGAAGGTGAACCAGGATTTGGAAATGTTGAAATTTCTTTAGGAAATGCAACTGATTATAA
GGGAAATAAATATCCAATACGTTATTCATCTGCTGATGGTACATTTATATATGATGGATT
AACTCAACGTACAACTTATGAATTTACATTTACCAATCCAAAAGATTATTACTTTGAAAC
CTATGAAAAGACATTGGCTTGTCCACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGC
TGATAGTTTGGCAGCAGATCTTTTAGTTGCAAATACCTTTGCCTATAACATTCCATTGAT
TAAACAAGTTGGTGTTTCATCAGGCTCATCTATACTTACAGTTAGAATTAGTGTTGATTT
GGGTTATGTTGAGCAAACTCAACCACCAACTCAACCACCTACTCAACCACCTACTCAACC
ACCAACACCTCCACCATTTACTGGTAGTGCCGTCGCTGGTTTATTATTTACCGATATCAA
TGGTAATGGAATTTTTGATGGAAGNNNNNNNNNN