>SHI254 (SHI254Q) /CSM/SH/SHI2-C/SHI254Q.Seq.d/
TCAAGATTACTCTATTCAATTCGTTGTCCTTGTACAACCATTCAAGAGAAACAAGCAGGT
TTGCCAACTAGTGGTGGTTGGTTTGGTGGTTCGTTCATTGATAATAGTATGGTTTTAATT
TGGAATAAAGAAGGCAGAGGTTTCCTTTATCGTATCGATCAAACCGATTTAAAATCTTTC
CCTTTAGGTGACCCTGTACCAATTGAAGGTACCTCACCATTATTATCAAGATCATATGGT
TCATCTCTATCCTCCTCTTCAAATTTAAATAATAGTTCTTTTAATATTAATAATAGCAAT
AGCAATAATAATAATAATAATAGTAATGGTAAAATTGGTCAACCAGATTATTCAATAATT
AATTTCACTTATCAATCAGGTATACCACCACCACAATTGTTAATAACTTTTACTTCAAAT
GATAATAATAATAGTCAACAAACACCATTATTAAAAATTGGAGCAGCAATGTGTTTTGGT
AATATATTTATGATTGGTGATGTTTATGGTCATATTAGAATTTGGGTAATACCAGTTAAT
AGTGAACAATTGTTAAATCCAACTAATCAGACAGCAACAACTAGTGGTAAGAGAATATTA
AGAGAACCAAATTTACAAGGATCAATATCTGATGGTTGGAAGXXXXXXXXXX

SRLLYSIRCPCTTIQEKQAGLPTSGGWFGGSFIDNSMVLIWNKEGRGFLYRIDQTDLKSF
PLGDPVPIEGTSPLLSRSYGSSLSSSSNLNNSSFNINNSNSNNNNNNSNGKIGQPDYSII
NFTYQSGIPPPQLLITFTSNDNNNSQQTPLLKIGAAMCFGNIFMIGDVYGHIRIWVIPVN
SEQLLNPTNQTATTSGKRILREPNLQGSISDGW---

Frame A:
SRLLYSIRCPCTTIQEKQAGLPTSGGWFGGSFIDNSMVLIWNKEGRGFLYRIDQTDLKSF
PLGDPVPIEGTSPLLSRSYGSSLSSSSNLNNSSFNINNSNSNNNNNNSNGKIGQPDYSII
NFTYQSGIPPPQLLITFTSNDNNNSQQTPLLKIGAAMCFGNIFMIGDVYGHIRIWVIPVN
SEQLLNPTNQTATTSGKRILREPNLQGSISDGW---


Frame B:
qdysiqfvvlvqpfkrnkqvcqlvvvglvvrsliivwf*fgikkaevsfivsikpi*nls
l*vtlyqlkvphhyyqdhmvhlypplqi*iivlliliiaiaiiiiiivmvklvnqiiq*l
islinqvyhhhnc**lllqmiiiivnkhhy*kleqqcvlviyl*lvmfmvilefg*yqli
vnnc*iqlirqqqlvvrey*enqiykdqylmvg---


Frame C:
kitlfnslslynhsretsrfan*wwlvwwfvh***ygfnle*rrqrfplsyrsnrfkifp
fr*pctn*ryltiiikiiwfisillfkfk**ff*y***q*q*******w*nwstrlfnn*
fhlsiryttttivnnfyfk*****stntiiknwssnvfw*yiydw*clwsy*nlgnts**
*tivksn*sdsnn*w*enikrtkftrini*wle---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHI289 (SHI289Q) /CSM/SH/SHI2-D/SHI289Q.Seq.d/                        624   e-178
SHI254 (SHI254Q) /CSM/SH/SHI2-C/SHI254Q.Seq.d/                        624   e-178
CHF594 (CHF594Q) /CSM/CH/CHF5-D/CHF594Q.Seq.d/                        230   2e-59
SSG887 (SSG887Q) /CSM/SS/SSG8-D/SSG887Q.Seq.d/                         40   0.041
SSG183 (SSG183Q) /CSM/SS/SSG1-D/SSG183Q.Seq.d/                         40   0.041
SLE582 (SLE582Q) /CSM/SL/SLE5-D/SLE582Q.Seq.d/                         40   0.041
CHQ285 (CHQ285Q) /CSM/CH/CHQ2-D/CHQ285Q.Seq.d/                         40   0.041
AFM867 (AFM867Q) /CSM/AF/AFM8-C/AFM867Q.Seq.d/                         40   0.041
VFI759 (VFI759Q) /CSM/VF/VFI7-C/VFI759Q.Seq.d/                         38   0.16
VFH618 (VFH618Q) /CSM/VF/VFH6-A/VFH618Q.Seq.d/                         38   0.16

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strainAX4, complete sequence.   624   0.0    2
AC160068|AC160068.2 Bos taurus clone CH240-87H4, *** SEQUENCING IN PROGRESS ***, 24 unordered pieces.    50   0.065  1
AC064852|AC064852.4 Homo sapiens BAC clone RP11-174L18 from 2, complete sequence.    50   0.065  1
CD074001|CD074001.1 MA3-0001U-M278-A11-U.G MA3-0001 Schistosoma mansoni cDNA clone MA3-0001U-M278-A11.G, mRNA sequence.    48   0.26   1
AB084781|AB084781.1 Mycoplasma mobile genes for P123, Gli349, Gli521, P42, complete cds.    36   0.43   5
AC173120|AC173120.2 Bos taurus clone CH240-493D3, *** SEQUENCING IN PROGRESS ***, 41 unordered pieces.    46   1.0    1
AY077832|AY077832.1 Sheeppox virus strain TU-V02127, complete genome.    46   1.0    1
AY077833|AY077833.1 Sheeppox virus A, complete genome.             46   1.0    1
AY077834|AY077834.1 Sheeppox virus NISKHI, complete genome.        46   1.0    1
AF325528|AF325528.1 Lumpy skin disease virus isolate Neethling 2490, complete genome.    46   1.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ138920_1(DQ138920|pid:none) Drosophila melanogaster strain Ral...    38   0.28
AL021086_3(AL021086|pid:none) Drosophila melanogaster cosmid clo...    38   0.28
DQ138923_1(DQ138923|pid:none) Drosophila yakuba strain Tai18 CG1...    38   0.28
DQ138925_1(DQ138925|pid:none) Drosophila erecta voucher stock #1...    38   0.28
DQ138922_1(DQ138922|pid:none) Drosophila simulans strain Ky-b CG...    38   0.28
BC129011_1(BC129011|pid:none) Xenopus tropicalis hypothetical pr...    34   3.1
AX244640_1(AX244640|pid:none) Sequence 3 from Patent WO0166739.        34   4.0
(Q9Y4E6) RecName: Full=WD repeat-containing protein 7; AltName: ...    34   4.0
AF188125_1(AF188125|pid:none) Homo sapiens TGF-beta resistance-a...    34   4.0
AB011113_1(AB011113|pid:none) Homo sapiens mRNA for KIAA0541 pro...    34   4.0

 psg: 0.72  gvh: 0.46  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHI254 is mit

>SHI254F.Seq
TCAAGATTACTCTATTCAATTCGTTGTCCTTGTACAACCATTCAAGAGAAACAAGCAGGT
TTGCCAACTAGTGGTGGTTGGTTTGGTGGTTCGTTCATTGATAATAGTATGGTTTTAATT
TGGAATAAAGAAGGCAGAGGTTTCCTTTATCGTATCGATCAAACCGATTTAAAATCTTTC
CCTTTAGGTGACCCTGTACCAATTGAAGGTACCTCACCATTATTATCAAGATCATATGGT
TCATCTCTATCCTCCTCTTCAAATTTAAATAATAGTTCTTTTAATATTAATAATAGCAAT
AGCAATAATAATAATAATAATAGTAATGGTAAAATTGGTCAACCAGATTATTCAATAATT
AATTTCACTTATCAATCAGGTATACCACCACCACAATTGTTAATAACTTTTACTTCAAAT
GATAATAATAATAGTCAACAAACACCATTATTAAAAATTGGAGCAGCAATGTGTTTTGGT
AATATATTTATGATTGGTGATGTTTATGGTCATATTAGAATTTGGGTAATACCAGTTAAT
AGTGAACAATTGTTAAATCCAACTAATCAGACAGCAACAACTAGTGGTAAGAGAATATTA
AGAGAACCAAATTTACAAGGATCAATATCTGATGGTTGGAAGNNNNNNNNNN