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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ548
Library AF
(Link to library)
Clone ID AFJ548
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U02855-1
Original site URL
Representative seq. ID AFJ548Z
(Link to Original site)
Representative DNA sequence 
>AFJ548 (AFJ548Q) /CSM/AF/AFJ5-B/AFJ548Q.Seq.d/
XXXXXXXXXXANGNNNCNGNANTTAAAAAAAGTTTTTTTTTTTNAAAGAATTNNNCNNNN
TNNNCNNCAAAANAANAANGGNTTTNGTTNNNANTTTCNGATAAAGAANNGATAAAGTNT
GTTTTNTTNCCNATNGAATTAAATAATAAATTCNATTTANAAGGTAATCAATTTTNCTAT
TGGNTGATGCNACAACNACAGGTAGNTCTTTNAAAATATATGAAAAAAATTTAAAAAAAT
TATGTAAAAATAATAATATTAATGTTAAAATTATAAAATTAGCAATTAAAAACCCAATAA
AAATAAAAAAACCAAAAATAAAAAAAATAAAATTAAAATAAAAATAAAAAAAAAATGATT
TATACTTGTTTTTCATATAACCATAATTCAAAAGGAAAAGAAAATCTAGAAATTGATAAT
TATAATAAATTATTAATATTAAAAGGTTATTCTAATATTAAAACTACTATTGAAAAACGA
AATAAAGCAATTTCTACAATAAAATCGTTATTTGAAGAAAAATTAAAAGAAATTGAATCA
ATAAATGGAAACATTGATTACATATTAGTATTACCAGGTCATCGTATTGGTTATTCAACT
TTTCAATATAGTATAAATAAATTCTATCAAAACGATATCGAACAACAACAACAACCACAA
CAACAACAACAACAACAACAACAACAACAACAAGTACCAGAAATTAAAAAAAAGTTTTTA
TATGTTAAAAGAATTAAACAAACTGAACAACAAAAGAAAGAAGGATTTCGTTCGAAATTA
TCAGATAAAGAAGAGATAAAGTCTGTTTTATTCCAATCGAATTAAATAATAAATTCAATT
TAGAAGGTAATCAATTTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---xxxxkksfffxknxxxxxxkxxxxxxxxsdkexikxvxxpxelnnkfxlxgnqfxyw
xmxqxqvxlxkymkki*knyvkiiilmlkl*n*qlktq*k*knqk*kk*n*NKNKKKMIY
TCFSYNHNSKGKENLEIDNYNKLLILKGYSNIKTTIEKRNKAISTIKSLFEEKLKEIESI
NGNIDYILVLPGHRIGYSTFQYSINKFYQNDIEQQQQPQQQQQQQQQQQQVPEIKKKFLY
VKRIKQTEQQKKEGFRSKLSDKEEIKSVLFQSN*iinsi*kvinl
Translated Amino Acid sequence (All Frames) 
Frame A:
---xxxx*kkfffxkexxxxxxkxxxxxvxxfx*rxdkvcfxxxxik**ixfxr*sixll
xdatttgxsxkiyeknlkklcknnninvkiiklaiknpikikkpkikkiklk*k*kkndl
ylffi*p*fkrkrksrn**l**iinikrlf*y*nyy*ktk*snfynkivi*rkikrn*in
kwkh*lhisitrssywlfnfsi*yk*ilskryrtttttttttttttttttstrn*kkvfi
c*kn*tn*ttkerrisfeiir*rrdkvcfipielnnkfnlegnqf


Frame B:
---xxxxkksfffxknxxxxxxkxxxxxxxxsdkexikxvxxpxelnnkfxlxgnqfxyw
xmxqxqvxlxkymkki*knyvkiiilmlkl*n*qlktq*k*knqk*kk*n*NKNKKKMIY
TCFSYNHNSKGKENLEIDNYNKLLILKGYSNIKTTIEKRNKAISTIKSLFEEKLKEIESI
NGNIDYILVLPGHRIGYSTFQYSINKFYQNDIEQQQQPQQQQQQQQQQQQVPEIKKKFLY
VKRIKQTEQQKKEGFRSKLSDKEEIKSVLFQSN*iinsi*kvinl


Frame C:
---xxxlkkvfffxrixxxxxqxxxgfxxxfxikxx*sxfxxxxn*iinsixkvinfxig
*cxnxr*xfxni*kkfkkim*k**y*c*nykisn*kpnknkktknkknkikikikkk*fi
lvfhitiiqkekki*kliiiiny*y*kvililklllkneikqflq*nrylkkn*kklnq*
metlity*yyqvivlviqlfniv*insiktisnnnnnhnnnnnnnnnnnkyqklkksfym
lkelnklnnkrkkdfvrnyqikkr*slfysnrik**iqfrr*si
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ548 (AFJ548Q) /CSM/AF/AFJ5-B/AFJ548Q.Seq.d/                        565   e-160
SSK646 (SSK646Q) /CSM/SS/SSK6-B/SSK646Q.Seq.d/                         46   9e-04
SSJ425 (SSJ425Q) /CSM/SS/SSJ4-B/SSJ425Q.Seq.d/                         46   9e-04
SSH608 (SSH608Q) /CSM/SS/SSH6-A/SSH608Q.Seq.d/                         46   9e-04
SSD344 (SSD344Q) /CSM/SS/SSD3-B/SSD344Q.Seq.d/                         46   9e-04
VHH455 (VHH455Q) /CSM/VH/VHH4-C/VHH455Q.Seq.d/                         44   0.003
VHE636 (VHE636Q) /CSM/VH/VHE6-B/VHE636Q.Seq.d/                         44   0.003
SHJ690 (SHJ690Q) /CSM/SH/SHJ6-D/SHJ690Q.Seq.d/                         44   0.003
SHI194 (SHI194Q) /CSM/SH/SHI1-D/SHI194Q.Seq.d/                         44   0.003
SHF508 (SHF508Q) /CSM/SH/SHF5-A/SHF508Q.Seq.d/                         44   0.003
own update 2002.10.29
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   565  0.0  9
AE014837|AE014837.1 Plasmodium falciparum 3D7 chromosome 11 section 2 of 8 of the complete sequence.    38  3e-04  11
AZ691084|AZ691084.1 ENTLH12TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44  7e-04  3
AE014828|AE014828.1 Plasmodium falciparum 3D7 chromosome 14 section 13 of 13 of the complete sequence.    44  0.003  11
AC006279|AC006279.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    34  0.013  9
AE014823|AE014823.1 Plasmodium falciparum 3D7 chromosome 14 section 8 of 13 of the complete sequence.    32  0.015  10
AL929354|AL929354.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 4/4.    34  0.015  11
BQ836536|BQ836536.1 rf47b06.y1 Meloidogyne hapla J2 pAMP1 v1 Meloidogyne hapla cDNA 5', mRNA sequence.    40  0.017  3
AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    42  0.023  10
L36900|L36900.1 Saccharomyces cerevisiae mitochondrion transfer RNA-Ser1 (tRNA-Ser) gene and varI gene, complete cds.    40  0.026  4
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116305_71(AC116305|pid:none) Dictyostelium discoideum chromoso...   263   1e-71
CP000141_646(CP000141|pid:none) Carboxydothermus hydrogenoforman...    35   3.0
(Q99109) RecName: Full=Repellent protein 1; Contains:   RecName:...    34   5.1
AE015927_481(AE015927|pid:none) Clostridium tetani E88, complete...    34   5.1
CR382135_99(CR382135|pid:none) Debaryomyces hansenii strain CBS7...    34   5.1
CP001205_570(CP001205|pid:none) Borrelia burgdorferi ZS7, comple...    34   5.1
AP008983_184(AP008983|pid:none) Clostridium phage c-st genomic D...    34   6.7
CP000939_2344(CP000939|pid:none) Clostridium botulinum B1 str. O...    33   8.7
protein update 2009. 4.23
PSORT 

 psg: 0.89  gvh: 0.31  alm: 0.51  top: 0.17  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ548 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFJ548Z
3' end seq. 
>AFJ548Z.Seq
----------ANGNNNCNGNANTTAAAAAAAGTTTTTTTTTTTNAAAGAATTNNNCNNNN
TNNNCNNCAAAANAANAANGGNTTTNGTTNNNANTTTCNGATAAAGAANNGATAAAGTNT
GTTTTNTTNCCNATNGAATTAAATAATAAATTCNATTTANAAGGTAATCAATTTTNCTAT
TGGNTGATGCNACAACNACAGGTAGNTCTTTNAAAATATATGAAAAAAATTTAAAAAAAT
TATGTAAAAATAATAATATTAATGTTAAAATTATAAAATTAGCAATTAAAAACCCAATAA
AAATAAAAAAACCAAAAATAAAAAAAATAAAATTAAAATAAAAATAAAAAAAAAATGATT
TATACTTGTTTTTCATATAACCATAATTCAAAAGGAAAAGAAAATCTAGAAATTGATAAT
TATAATAAATTATTAATATTAAAAGGTTATTCTAATATTAAAACTACTATTGAAAAACGA
AATAAAGCAATTTCTACAATAAAATCGTTATTTGAAGAAAAATTAAAAGAAATTGAATCA
ATAAATGGAAACATTGATTACATATTAGTATTACCAGGTCATCGTATTGGTTATTCAACT
TTTCAATATAGTATAAATAAATTCTATCAAAACGATATCGAACAACAACAACAACCACAA
CAACAACAACAACAACAACAACAACAACAACAAGTACCAGAAATTAAAAAAAAGTTTTTA
TATGTTAAAAGAATTAAACAAACTGAACAACAAAAGAAAGAAGGATTTCGTTCGAAATTA
TCAGATAAAGAAGAGATAAAGTCTGTTTTATTCCAATCGAATTAAATAATAAATTCAATT
TAGAAGGTAATCAATTTA
Length of 3' end seq. 848
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -