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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ532
Library AF
(Link to library)
Clone ID AFJ532
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFJ532P
(Link to Original site)
Representative DNA sequence 
>AFJ532 (AFJ532Q) /CSM/AF/AFJ5-B/AFJ532Q.Seq.d/
AAATTAATTATTTTTTTTATCTTCATTATATACAATTTAATTTCATTAATTTTTTAATGG
CAAAACTAAAAATAATTATTTATATTGTAATAATTTTTTGTAGTAGTATCCAATTTTCTT
TTGGATACGAAAAGGCGATTGCTTCATTTTATCCACCTCCAGGTGATTTGCATCCCACTA
CTCATAATTTTTGGGCATGTAATAGTGTCATTAACCAAAACGGTGGATTCGGTCCACTTG
AATGCAATAATGCCGGTATCTATGCAATTTTCTCGACCGCCAATGAGATCATCGACGGTA
ATGAATACATGCTTCCACTATTTTATATTGGAAGATCCACTATAGATGTTCGAGATAGAA
TTGCTGATCACAGTAGACGAAACCATGTTGATAATGATTTCATTGTGTGTTATATGCGTT
TCCCAGCATTGGATGATTATGGCTACAGTAACAAAGTAACACCCGTTGAACAAGCTCTCC
TTCACCAATTCAAAACTCAATTCAACTCATGGGATAACCTCGGTTTTGATCCACAGNATG
ATACTGCTTATCCAGATGAAAATGGTTTGGGATGGGATATXXXXXXXXXXAGCTGGCAAC
GGNGCACGTAATTATGCTAATNAAGGTTACAATTCCGTNAAANATGGTGCACAAAAAGCT
GGCAACGGNGCNCGTAATTATGCTCAACAAGGTTNTCAAGCATGGANANAAGGNGTCAAT
NANATTGGTGATGATATCGCCAATACTGCNCATNATGTAGGTGATGGTTTNGCCAATACT
GCACGTTAAGTAGGTGATGGAATTANAATCCCTGCANAGGAGGGATATNAACTCATTAAA
NATGGAGCTGAANAAACTTGGGTGAANAAATATTAAAGGTTATCCTTNATTAAAATTNAA
AAAGCCCAACAAAGAATGGCTNAACAANAANAAANAANAAGGTTTATGCAANAANAAAGT
NAAANAAGACGATTGCAAANAAACANANAAAGACNAGAGCC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
INYFFYLHYIQFNFINFLMAKLKIIIYIVIIFCSSIQFSFGYEKAIASFYPPPGDLHPTT
HNFWACNSVINQNGGFGPLECNNAGIYAIFSTANEIIDGNEYMLPLFYIGRSTIDVRDRI
ADHSRRNHVDNDFIVCYMRFPALDDYGYSNKVTPVEQALLHQFKTQFNSWDNLGFDPQXD
TAYPDENGLGWD---

---AGNGARNYANXGYNSVKXGAQKAGNGARNYAQQGXQAWXXGVNXIGDDIANTAHXVG
DGXANTAR*vgdgixipaxegyxlikxgaextwvxky*rlsxikixkaqqrmaxqxxxxr
fmqxxsxxrrlqxnxxrxe
Translated Amino Acid sequence (All Frames) 
Frame A:
kliiffifiiynlislif*wqn*k*lfil**ffvvvsnflldtkrrllhfihlqvicipl
liifghvivsltktvdsvhlnaimpvsmqfsrppmrsstvmntcfhyfiledpl*mfeie
llitvdetmlimislcvicvsqhwmimatvtk*hplnklsftnsklnsthgitsvlihxm
illiqmkmvwdgi---

---swqrxt*lc*xrlqfrxxwctkswqrxx*lcstrxssmxxrxqxxw**yrqycxxcr
*wfxqyctlsr*wnxnpcxggixth*xws*xnlgexilkvilx*nxksptkngxtxxxxk
vyaxxkxxktiaxkxxktra

Frame B:
n*lfflsslyti*fh*ffngktknnylycnnfl**ypiffwirkgdcfilstsr*fashy
s*flgm**ch*pkrwirst*mq*crylcnfldrq*dhrr**ihastilywkihyrcsr*n
c*sq*tkpc***fhcvlyafpsig*lwlq*qsntr*tsspspiqnsiqlmg*prf*stx*
yclsr*kwfgmgy---

---AGNGARNYANXGYNSVKXGAQKAGNGARNYAQQGXQAWXXGVNXIGDDIANTAHXVG
DGXANTAR*vgdgixipaxegyxlikxgaextwvxky*rlsxikixkaqqrmaxqxxxxr
fmqxxsxxrrlqxnxxrxe

Frame C:
INYFFYLHYIQFNFINFLMAKLKIIIYIVIIFCSSIQFSFGYEKAIASFYPPPGDLHPTT
HNFWACNSVINQNGGFGPLECNNAGIYAIFSTANEIIDGNEYMLPLFYIGRSTIDVRDRI
ADHSRRNHVDNDFIVCYMRFPALDDYGYSNKVTPVEQALLHQFKTQFNSWDNLGFDPQXD
TAYPDENGLGWD---

---latxhvimlxkvtipxxmvhkklatxxvimlnkvxkhgxkxsxxlvmispilxxm*v
mvxpilhvk*vmelxslxrrdxnslxmelxklg*xnikgypxlkxkkpnkewlnxxkxxg
lcxxkvkxddckxtxkdxs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ532 (AFJ532Q) /CSM/AF/AFJ5-B/AFJ532Q.Seq.d/                       1532   0.0
SFH254 (SFH254Q) /CSM/SF/SFH2-C/SFH254Q.Seq.d/                       1100   0.0
SFF507 (SFF507Q) /CSM/SF/SFF5-A/SFF507Q.Seq.d/                       1100   0.0
CHR880 (CHR880Q) /CSM/CH/CHR8-D/CHR880Q.Seq.d/                       1100   0.0
CHG883 (CHG883Q) /CSM/CH/CHG8-D/CHG883Q.Seq.d/                       1100   0.0
CHD331 (CHD331Q) /CSM/CH/CHD3-B/CHD331Q.Seq.d/                       1100   0.0
CHC680 (CHC680Q) /CSM/CH/CHC6-D/CHC680Q.Seq.d/                       1100   0.0
CHC117 (CHC117Q) /CSM/CH/CHC1-A/CHC117Q.Seq.d/                       1100   0.0
CHA432 (CHA432Q) /CSM/CH/CHA4-B/CHA432Q.Seq.d/                       1100   0.0
CFF683 (CFF683Q) /CSM/CF/CFF6-D/CFF683Q.Seq.d/                       1100   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117081|AC117081.1 Dictyostelium discoideum chromosome 2 map 4903137-4960091 strain AX4, *** SEQUENCING IN PROGRESS ***.    50  0.046  3
AW076871|AW076871.1 fj03a01.y1 Zebrafish adult olfactory Danio rerio cDNA 5', mRNA sequence.    50  0.055  1
BH689643|BH689643.1 BOMDR69TF BO_2_3_KB Brassica oleracea genomic clone BOMDR69, DNA sequence.    50  0.055  1
BH530565|BH530565.1 BOGJR47TF BOGJ Brassica oleracea genomic clone BOGJR47, DNA sequence.    50  0.055  1
AC021820|AC021820.7 Homo sapiens chromosome , clone RP11-40H19, complete sequence.    46  0.23  2
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    50  0.81  2
AC009640|AC009640.3 Homo sapiens clone RP11-71B15, WORKING DRAFT SEQUENCE, 17 unordered pieces.    46  0.86  1
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    50  0.95  2
AC116979|AC116979.2 Dictyostelium discoideum chromosome 2 map 6357117-6445670 strain AX4, complete sequence.    40  1.1  4
AX344740|AX344740.1 Sequence 165 from Patent WO0200927.           38  2.3  2
dna update 2003. 9. 5
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 1,328,145,687
protein update 2009. 4.23
PSORT 

 psg: 0.87  gvh: 0.62  alm: 0.22  top: 0.37  tms: 0.07  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.88  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFJ532 is cyt
5' end seq. ID AFJ532F
5' end seq. 
>AFJ532F.Seq
AAATTAATTATTTTTTTTATCTTCATTATATACAATTTAATTTCATTAATTTTTTAATGG
CAAAACTAAAAATAATTATTTATATTGTAATAATTTTTTGTAGTAGTATCCAATTTTCTT
TTGGATACGAAAAGGCGATTGCTTCATTTTATCCACCTCCAGGTGATTTGCATCCCACTA
CTCATAATTTTTGGGCATGTAATAGTGTCATTAACCAAAACGGTGGATTCGGTCCACTTG
AATGCAATAATGCCGGTATCTATGCAATTTTCTCGACCGCCAATGAGATCATCGACGGTA
ATGAATACATGCTTCCACTATTTTATATTGGAAGATCCACTATAGATGTTCGAGATAGAA
TTGCTGATCACAGTAGACGAAACCATGTTGATAATGATTTCATTGTGTGTTATATGCGTT
TCCCAGCATTGGATGATTATGGCTACAGTAACAAAGTAACACCCGTTGAACAAGCTCTCC
TTCACCAATTCAAAACTCAATTCAACTCATGGGATAACCTCGGTTTTGATCCACAGNATG
ATACTGCTTATCCAGATGAAAATGGTTTGGGATGGGATAT----------
Length of 5' end seq. 580
3' end seq. ID AFJ532Z
3' end seq. 
>AFJ532Z.Seq
----------AGCTGGCAACGGNGCACGTAATTATGCTAATNAAGGTTACAATTCCGTNA
AANATGGTGCACAAAAAGCTGGCAACGGNGCNCGTAATTATGCTCAACAAGGTTNTCAAG
CATGGANANAAGGNGTCAATNANATTGGTGATGATATCGCCAATACTGCNCATNATGTAG
GTGATGGTTTNGCCAATACTGCACGTTAAGTAGGTGATGGAATTANAATCCCTGCANAGG
AGGGATATNAACTCATTAAANATGGAGCTGAANAAACTTGGGTGAANAAATATTAAAGGT
TATCCTTNATTAAAATTNAAAAAGCCCAACAAAGAATGGCTNAACAANAANAAANAANAA
GGTTTATGCAANAANAAAGTNAAANAAGACGATTGCAAANAAACANANAAAGACNAGAGC
C
Length of 3' end seq. 411
Connected seq. ID AFJ532P
Connected seq. 
>AFJ532P.Seq
AAATTAATTATTTTTTTTATCTTCATTATATACAATTTAATTTCATTAATTTTTTAATGG
CAAAACTAAAAATAATTATTTATATTGTAATAATTTTTTGTAGTAGTATCCAATTTTCTT
TTGGATACGAAAAGGCGATTGCTTCATTTTATCCACCTCCAGGTGATTTGCATCCCACTA
CTCATAATTTTTGGGCATGTAATAGTGTCATTAACCAAAACGGTGGATTCGGTCCACTTG
AATGCAATAATGCCGGTATCTATGCAATTTTCTCGACCGCCAATGAGATCATCGACGGTA
ATGAATACATGCTTCCACTATTTTATATTGGAAGATCCACTATAGATGTTCGAGATAGAA
TTGCTGATCACAGTAGACGAAACCATGTTGATAATGATTTCATTGTGTGTTATATGCGTT
TCCCAGCATTGGATGATTATGGCTACAGTAACAAAGTAACACCCGTTGAACAAGCTCTCC
TTCACCAATTCAAAACTCAATTCAACTCATGGGATAACCTCGGTTTTGATCCACAGNATG
ATACTGCTTATCCAGATGAAAATGGTTTGGGATGGGATAT----------AGCTGGCAAC
GGNGCACGTAATTATGCTAATNAAGGTTACAATTCCGTNAAANATGGTGCACAAAAAGCT
GGCAACGGNGCNCGTAATTATGCTCAACAAGGTTNTCAAGCATGGANANAAGGNGTCAAT
NANATTGGTGATGATATCGCCAATACTGCNCATNATGTAGGTGATGGTTTNGCCAATACT
GCACGTTAAGTAGGTGATGGAATTANAATCCCTGCANAGGAGGGATATNAACTCATTAAA
NATGGAGCTGAANAAACTTGGGTGAANAAATATTAAAGGTTATCCTTNATTAAAATTNAA
AAAGCCCAACAAAGAATGGCTNAACAANAANAAANAANAAGGTTTATGCAANAANAAAGT
NAAANAAGACGATTGCAAANAAACANANAAAGACNAGAGCC
Length of connected seq. 991
Full length Seq ID -
Full length Seq. -
Length of full length seq. -