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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ512
Library AF
(Link to library)
Clone ID AFJ512
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16444-1
Original site URL
Representative seq. ID AFJ512E
(Link to Original site)
Representative DNA sequence 
>AFJ512 (AFJ512Q) /CSM/AF/AFJ5-A/AFJ512Q.Seq.d/
ATTTAATTTTAAACAAAGAGTGGATATTTTCTAAAAAAAATAAAAATAAAAATGGAAATG
GAAGGATTTGATCATGTTACATTTTGGGTTGGTAATGCATTACAAGCAGCAACTTATTAC
ATTGCTAGATTTGGATTTCAAAATTTAGCTTATAGTGGTTTAGAAACTGGTAATCGTCAA
TTCGCCACTCATGTTATCCATCAAAACAACATTATTATGGCTTTTACATCACCATTAACT
GGTGACAACAAAGATTATGCAGACCACATGATGAGACATGGTGATGGTGTCAAAGATATT
GCTTTCAACGTTAAAGATGTACAACACATTTATGATGAAGCAGTTAAAGCAGGAGCTCAA
TCAGTTAAAGAACCACATCAAATTAAAGACGAACATGGTATTGTTACATTAGCAACTATC
ATGAGTCCATATGGTGAAACTACACATACTTTTGTTGATAGATCTCAATATAAAGGTGCA
TTCTTACCAGGTTTCACATACAAGGTCGCTTCAGATCCATTATCAAATATCACCGAACCA
GTTGGCCTCAACTTAATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATGGAACCC
AGTCGTTCAATGGTACGAAAAGGTTTTACAATTCCACCGTTTCTGGTCGGTTGATGACAA
AACCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTGAAAA
AGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAGAATA
CGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACATCAT
TGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAACATA
CTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATTTGGA
CACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTTTTACAAAT
CTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAAACAA
CCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGACAACA
AGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATATAACTAAAT
AACATTACAATATATCAAAATAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
i*f*TKSGYFLKKIKIKMEMEGFDHVTFWVGNALQAATYYIARFGFQNLAYSGLETGNRQ
FATHVIHQNNIIMAFTSPLTGDNKDYADHMMRHGDGVKDIAFNVKDVQHIYDEAVKAGAQ
SVKEPHQIKDEHGIVTLATIMSPYGETTHTFVDRSQYKGAFLPGFTYKVASDPLSNITEP
VGLNLIDHVVSNHADKMMEPSRSMVRKGFTIPPFLVG**qnhsyrifiikisrsc**v*k
s*itn**tsqwy*kesnsrirrflqwcwcstyrlkd**hh*cylkikiswclfphcskni
lyitqreittlfirn*rrfghfreithfnrl**qrlsftnlyk*c*r*tncfl*nypkkq
p*wfrcw*l*ipl*snrkttrnswkligvsqlqmffi*lnnitiyqn
Translated Amino Acid sequence (All Frames) 
Frame A:
i*f*TKSGYFLKKIKIKMEMEGFDHVTFWVGNALQAATYYIARFGFQNLAYSGLETGNRQ
FATHVIHQNNIIMAFTSPLTGDNKDYADHMMRHGDGVKDIAFNVKDVQHIYDEAVKAGAQ
SVKEPHQIKDEHGIVTLATIMSPYGETTHTFVDRSQYKGAFLPGFTYKVASDPLSNITEP
VGLNLIDHVVSNHADKMMEPSRSMVRKGFTIPPFLVG**qnhsyrifiikisrsc**v*k
s*itn**tsqwy*kesnsrirrflqwcwcstyrlkd**hh*cylkikiswclfphcskni
lyitqreittlfirn*rrfghfreithfnrl**qrlsftnlyk*c*r*tncfl*nypkkq
p*wfrcw*l*ipl*snrkttrnswkligvsqlqmffi*lnnitiyqn


Frame B:
fnfkqrvdif*kk*k*kwkwkdlimlhfglvmhykqqlitlldldfki*livv*klvivn
splmlsikttllwllhhh*lvttkimqtt**dmvmvskillstlkmyntfmmkqlkqeln
qlknhiklktnmvllh*qls*vhmvklhilllidlnikvhsyqvshtrslqihyqispnq
last**imsfqimqik*wnpvvqwyekvlqfhrfwsvddktihteysslrsvvvadksek
vklpinepangirksqiqeyvdfyngagvqhialktdniidaisklrsrgvsfltvpkty
ytslreklqhssleikedldtleklhilidyddkgyllqiftnnvedkptvffeiiqrnn
hdgfgagnfkslfeaierqqetrgnl*vclnykcslyn*itlqyiki


Frame C:
lilnkewifskknknkngngri*scyilgw*citssnllhc*iwiskfsl*wfrnw*ssi
rhscypskqhyygfyitinw*qqrlcrphdetw*wcqrycfqr*rctthl**ss*srssi
s*rttsn*rrtwycyisnyhesiw*nytyfc**isi*rciltrfhiqgrfrsiikyhrts
wpqlnrscrfkscr*ndgtqsfngtkrfynstvsgrlmtkpfipnihh*dqs*llislkk
lnyqlmnqpmvlervkfknt*istmvlvfnisp*rlitslmlsqn*dlvvslsslfqkht
ihhsernyntlh*klkkiwtl*rnytf*simmtkvifykslqimlkinqlfslklskett
mmvsvlvtlnpslkqskdnkklvetyrcvsitnvlyitk*hynisk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ512 (AFJ512Q) /CSM/AF/AFJ5-A/AFJ512Q.Seq.d/                       2321   0.0
CFG888 (CFG888Q) /CSM/CF/CFG8-D/CFG888Q.Seq.d/                       2298   0.0
CFB319 (CFB319Q) /CSM/CF/CFB3-A/CFB319Q.Seq.d/                       2284   0.0
CFA389 (CFA389Q) /CSM/CF/CFA3-D/CFA389Q.Seq.d/                       2276   0.0
VFC717 (VFC717Q) /CSM/VF/VFC7-A/VFC717Q.Seq.d/                       2272   0.0
SFE524 (SFE524Q) /CSM/SF/SFE5-A/SFE524Q.Seq.d/                       2272   0.0
CHD784 (CHD784Q) /CSM/CH/CHD7-D/CHD784Q.Seq.d/                       2272   0.0
CFJ240 (CFJ240Q) /CSM/CF/CFJ2-B/CFJ240Q.Seq.d/                       2272   0.0
CFF757 (CFF757Q) /CSM/CF/CFF7-C/CFF757Q.Seq.d/                       2272   0.0
CFF524 (CFF524Q) /CSM/CF/CFF5-A/CFF524Q.Seq.d/                       2272   0.0
own update 2002.11. 5
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.  1166   0.0    11
BI322343|BI322343.1 kx19h02.y3 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    42   3e-09  4
CO013595|CO013595.1 EST801930 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBZ67 5' end, mRNA sequence.    52   1e-08  3
CO009511|CO009511.1 EST797846 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBC24 5' end, mRNA sequence.    52   1e-08  3
L38493|L38493.1 Coccidioides immitis T-cell reactive protein (trcP) gene exons 1-4, complete cds.    52   6e-08  3
CO006156|CO006156.1 EST794491 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAT15 3' end, mRNA sequence.    52   1e-07  2
CO005676|CO005676.1 EST794011 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAQ39 3' end, mRNA sequence.    52   2e-07  2
CO009510|CO009510.1 EST797845 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBC24 3' end, mRNA sequence.    52   2e-07  2
CO005677|CO005677.1 EST794012 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAQ39 5' end, mRNA sequence.    52   2e-07  3
BM395514|BM395514.1 50072-2-9-E12.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    64   6e-06  1
dna update 2004. 7.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NV5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   377   0.0
AE014296_3563(AE014296|pid:none) Drosophila melanogaster chromos...   270   e-118
BC077167_1(BC077167|pid:none) Danio rerio 4-hydroxyphenylpyruvat...   264   e-116
S32821(S32821;S35890;S35889)4-hydroxyphenylpyruvate dioxygenase ...   264   e-115
BC153801_1(BC153801|pid:none) Xenopus laevis hypothetical protei...   264   e-114
(Q5EA20) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   262   e-112
(Q6TGZ5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   268   e-112
(P32754) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   263   e-111
BT045841_1(BT045841|pid:none) Salmo salar clone ssal-rgf-534-157...   257   e-111
BC046075_1(BC046075|pid:none) Danio rerio zgc:56326, mRNA (cDNA ...   268   e-111
protein update 2009. 6.29
PSORT 

 psg: 0.64  gvh: 0.43  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFJ512 is cyt
5' end seq. ID AFJ512F
5' end seq. 
>AFJ512F.Seq
ATTTAATTTTAAACAAAGAGTGGATATTTTCTAAAAAAAATAAAAATAAAAATGGAAATG
GAAGGATTTGATCATGTTACATTTTGGGTTGGTAATGCATTACAAGCAGCAACTTATTAC
ATTGCTAGATTTGGATTTCAAAATTTAGCTTATAGTGGTTTAGAAACTGGTAATCGTCAA
TTCGCCACTCATGTTATCCATCAAAACAACATTATTATGGCTTTTACATCACCATTAACT
GGTGACAACAAAGATTATGCAGACCACATGATGAGACATGGTGATGGTGTCAAAGATATT
GCTTTCAACGTTAAAGATGTACAACACATTTATGATGAAGCAGTTAAAGCAGGAGCTCAA
TCAGTTAAAGAACCACATCAAATTAAAGACGAACATGGTATTGTTACATTAGCAACTATC
ATGAGTCCATATGGTGAAACTACACATACTTTTGTTGATAGATCTCAATATAAAGGTGCA
TTCTTACCAGGTTTCACATACAAGGTCGCTTCAGATCCATTATCAAATATCACCGAACCA
GTTGGCCTCAACTTAATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATGGAACCC
AGTCGTTCAATGGTACGAAAA----------
Length of 5' end seq. 621
3' end seq. ID AFJ512Z
3' end seq. 
>AFJ512Z.Seq
----------TCGCTTCAGATCCATTATCAAATATCACCGAACCAGTTGGCCTCAACTTA
ATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATGGAACCAGTCGTTCAATGGTAC
GAAAAGGTTTTACAATTCCACCGTTTCTGGTCGGTTGATGACAAAACCATTCATACCGAA
TATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTGAAAAAGTTAAATTACCAATT
AATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAGAATACGTAGATTTCTACAAT
GGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACATCATTGATGCTATCTCAAAA
TTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAACATACTATACATCACTCAGA
GAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATTTGGACACTTTAGAGAAATTA
CACATTTTAATCGATTATGATGACAAAGGTTATCTTTTACAAATCTTTACAAATAATGTT
GAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAAACAACCATGATGGTTTCGGT
GCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGACAACAAGAAACTCGTGGAAAC
TTATAGGTGTGTCTCAATTACAAATGTTCTTTATATAACTAAATAACATTACAATATATC
AAAATAA
Length of 3' end seq. 717
Connected seq. ID AFJ512P
Connected seq. 
>AFJ512P.Seq
ATTTAATTTTAAACAAAGAGTGGATATTTTCTAAAAAAAATAAAAATAAAAATGGAAATG
GAAGGATTTGATCATGTTACATTTTGGGTTGGTAATGCATTACAAGCAGCAACTTATTAC
ATTGCTAGATTTGGATTTCAAAATTTAGCTTATAGTGGTTTAGAAACTGGTAATCGTCAA
TTCGCCACTCATGTTATCCATCAAAACAACATTATTATGGCTTTTACATCACCATTAACT
GGTGACAACAAAGATTATGCAGACCACATGATGAGACATGGTGATGGTGTCAAAGATATT
GCTTTCAACGTTAAAGATGTACAACACATTTATGATGAAGCAGTTAAAGCAGGAGCTCAA
TCAGTTAAAGAACCACATCAAATTAAAGACGAACATGGTATTGTTACATTAGCAACTATC
ATGAGTCCATATGGTGAAACTACACATACTTTTGTTGATAGATCTCAATATAAAGGTGCA
TTCTTACCAGGTTTCACATACAAGGTCGCTTCAGATCCATTATCAAATATCACCGAACCA
GTTGGCCTCAACTTAATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATGGAACCC
AGTCGTTCAATGGTACGAAAA----------TCGCTTCAGATCCATTATCAAATATCACC
GAACCAGTTGGCCTCAACTTAATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATG
GAACCAGTCGTTCAATGGTACGAAAAGGTTTTACAATTCCACCGTTTCTGGTCGGTTGAT
GACAAAACCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCT
GAAAAAGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAA
GAATACGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAAC
ATCATTGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAA
ACATACTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGAT
TTGGACACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTTTTA
CAAATCTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGA
AACAACCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGA
CAACAAGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATATAAC
TAAATAACATTACAATATATCAAAATAA
Length of connected seq. 1338
Full length Seq ID AFJ512E
Full length Seq. 
>AFJ512E.Seq
ATTTAATTTTAAACAAAGAGTGGATATTTTCTAAAAAAAATAAAAATAAAAATGGAAATG
GAAGGATTTGATCATGTTACATTTTGGGTTGGTAATGCATTACAAGCAGCAACTTATTAC
ATTGCTAGATTTGGATTTCAAAATTTAGCTTATAGTGGTTTAGAAACTGGTAATCGTCAA
TTCGCCACTCATGTTATCCATCAAAACAACATTATTATGGCTTTTACATCACCATTAACT
GGTGACAACAAAGATTATGCAGACCACATGATGAGACATGGTGATGGTGTCAAAGATATT
GCTTTCAACGTTAAAGATGTACAACACATTTATGATGAAGCAGTTAAAGCAGGAGCTCAA
TCAGTTAAAGAACCACATCAAATTAAAGACGAACATGGTATTGTTACATTAGCAACTATC
ATGAGTCCATATGGTGAAACTACACATACTTTTGTTGATAGATCTCAATATAAAGGTGCA
TTCTTACCAGGTTTCACATACAAGGTCGCTTCAGATCCATTATCAAATATCACCGAACCA
GTTGGCCTCAACTTAATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATGGAACCC
AGTCGTTCAATGGTACGAAAAGGTTTTACAATTCCACCGTTTCTGGTCGGTTGATGACAA
AACCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTGAAAA
AGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAGAATA
CGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACATCAT
TGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAACATA
CTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATTTGGA
CACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTTTTACAAAT
CTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAAACAA
CCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGACAACA
AGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATATAACTAAAT
AACATTACAATATATCAAAATAA
Length of full length seq. 1223