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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ501
Library AF
(Link to library)
Clone ID AFJ501
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15531-1
Original site URL
Representative seq. ID AFJ501P
(Link to Original site)
Representative DNA sequence 
>AFJ501 (AFJ501Q) /CSM/AF/AFJ5-A/AFJ501Q.Seq.d/
CATTTTAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGCTAAC
ATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAGAAAA
TTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGTTGTT
GGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCATAAT
AAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGAAAAT
TCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGTTTTC
CTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTACTATG
CTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTATTGAT
TTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGCCAAT
TCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTAGAGGGTTATGTTGTAGCAATC
TCACCATTTAATTTCACAGCAATTGGTTTGAATTTAAGTTCAGCCCCAGCTTTAATGGGT
AATGTTGTCCTTTGGAAGCCTGCTTCAACTGCTGTCTTATCXXXXXXXXXXAATTAAAGA
TAGATTAGGTTACTGGTGTTAAATCAAATGAAAAATGGGTCAATCTGATGATTTCTCTTC
ATTCGTTTCAGCTGTCATCGATAAAAACTCTTTCAACAATATTCAATCATACATTGAACA
CGCTAAAGCTTCACCAGATGCTGAAATCATTGTCGGTGGTAAATGTGATAGCTCTGTTGG
TTGGTTTGTTGAACCAACTATCATCCTTGCCAAAGATCCACATTACAAATCAATGGAAGA
AGAAATTTTCGGTCCAGTCTTAACCATTTATGTTTATGAAGATTCTAAATTTGAAGAAAC
ATTAAAAATCTGTGATGAAACTTCACCATATGCTTTAACTGGTTCAATCTTTTCAACTTG
TAGATATGCTATTGAAACCGCTCACAAATACTTGAAGAATGCTGCTGGTAACTTTTATAT
CAATGATAAATGCACTGGTGCCGTCGTTGGTCAACAACCATTTGGTGGTTCTCGTGCTTC
AGGTACAAACGATAAAGCTGGTTCATCATTAAATCTTTTAAGATGGATCAGCGCCAGAAC
AATCAAAGAAAACTTTGTCCCACTCACAGTTTCACTATCCATACAGATNATCCAGAAAAC
TAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
hfkh*KNTSRVLMSHFARANIPTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIPCVV
GGKEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARSAVF
LKAADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQPPAN
SAGCWNILTYQPLEGYVVAISPFNFTAIGLNLSSAPALMGNVVLWKPASTAVL---

---IKDRLGYWC*ik*kmgqsddfssfvsavidknsfnniqsyiehakaspdaeiivggk
cdssvgwfveptiilakdphyksmeeeifgpvltiyvyedskfeetlkicdetspyaltg
sifstcryaietahkylknaagnfyindkctgavvgqqpfggsrasgtndkagsslnllr
wisartikenfvpltvslsiqxiqkt
Translated Amino Acid sequence (All Frames) 
Frame A:
hfkh*KNTSRVLMSHFARANIPTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIPCVV
GGKEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARSAVF
LKAADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQPPAN
SAGCWNILTYQPLEGYVVAISPFNFTAIGLNLSSAPALMGNVVLWKPASTAVL---

---n*r*irllvlnqmkngsi**flfirfschr*klfqqysiih*tr*sftrc*nhcrw*
m**lcwlvc*tnyhpcqrstlqingrrnfrsslnhlcl*rf*i*rniknl**nfticfnw
fnlfnl*icy*nrsqileeccw*llyq**mhwcrrwsttiwwfscfrykr*swfiiksfk
mdqrqnnqrklcpthsftihtdxpen*

Frame B:
ilnikkthqeyscltlqeltsqhrltnqc*imklvqhieny*kkhvlnletqqsifhvll
vvkkfvlvmfksn*faliiikywppsikpmpnysn*plkipwnlkpngnhyhlkqevqfs
lklqic*ipstvmms*lllclvkvklfgkqklmqqqkvlissvsmlntlkkftnnnhqpi
lqavgtf*pinh*rvml*qshhlisqqlv*i*vqpql*wvmlsfgsllqllsy---

---IKDRLGYWC*ik*kmgqsddfssfvsavidknsfnniqsyiehakaspdaeiivggk
cdssvgwfveptiilakdphyksmeeeifgpvltiyvyedskfeetlkicdetspyaltg
sifstcryaietahkylknaagnfyindkctgavvgqqpfggsrasgtndkagsslnllr
wisartikenfvpltvslsiqxiqkt

Frame C:
f*tlkkhiksthvslcks*hpntg*ptnvkl*swfnt*kiikrsmc*i*khnnrysmccw
w*rnsyw*cskainll*s**sighlpssqcritqisr*kfhgi*spmgiiti*skkcsfp
*scrfvkyqvpl*clsfyyawsr*nclasrn*cssrry*fppfqc*irsrnlpttttsqf
crllehfnlstirglccsnlti*fhsnwfefkfspsfng*ccpleacfnccli---

---lkid*vtgvksnekwvnlmislhsfqlssiktlstifnhtlntlklhqmlkslsvvn
viallvgllnqlsslpkihitnqwkkkfsvqs*pfmfmkilnlkkh*ksvmklhhml*lv
qsfqlvdmllkpltnt*rmllvtfisminalvpslvnnhlvvlvlqvqtiklvhh*if*d
gsapeqskktlshsqfhypyrxsrkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ501 (AFJ501Q) /CSM/AF/AFJ5-A/AFJ501Q.Seq.d/                       2518   0.0
SLH659 (SLH659Q) /CSM/SL/SLH6-C/SLH659Q.Seq.d/                       1338   0.0
AFD340 (AFD340Q) /CSM/AF/AFD3-B/AFD340Q.Seq.d/                       1233   0.0
VFN208 (VFN208Q) /CSM/VF/VFN2-A/VFN208Q.Seq.d/                       1227   0.0
AFF439 (AFF439Q) /CSM/AF/AFF4-B/AFF439Q.Seq.d/                       1219   0.0
VFE114 (VFE114Q) /CSM/VF/VFE1-A/VFE114Q.Seq.d/                       1207   0.0
AFN524 (AFN524Q) /CSM/AF/AFN5-A/AFN524Q.Seq.d/                       1197   0.0
AFB584 (AFB584Q) /CSM/AF/AFB5-D/AFB584Q.Seq.d/                       1156   0.0
AFK401 (AFK401Q) /CSM/AF/AFK4-A/AFK401Q.Seq.d/                       1138   0.0
VFK367 (VFK367Q) /CSM/VF/VFK3-C/VFK367Q.Seq.d/                       1132   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL031786|AL031786.1 S.pombe chromosome II cosmid c24C6.           88  1e-14  4
D89230|D89230.1 Schizosaccharomyces pombe mRNA, partial cds, clone: SY 1373.    80  8e-11  1
CF192429|CF192429.1 l4g02j2.f1 Cryptococcus neoformans strain B3501 Cryptococcus neoformans var. neoformans cDNA clone l4g02j2 3', mRNA sequence.    70  8e-08  1
AL416312|AL416312.1 T7 end of clone AX0AA013D07 of library AX0AA from strain CBS 7064 of Pichia farinosa.    48  1e-06  3
AL416406|AL416406.1 T3 end of clone AX0AA014C08 of library AX0AA from strain CBS 7064 of Pichia farinosa.    56  1e-05  2
AL393468|AL393468.1 T3 end of clone AR0AA011B08 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    60  8e-05  1
AC090078|AC090078.2 Trypanosoma cruzi clone GAMFS45 strain CL Brener, *** SEQUENCING IN PROGRESS ***, 12 unordered pieces.    60  9e-05  2
AZ932439|AZ932439.1 474.dhz99a06.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz99a06.s1, DNA sequence.    58  3e-04  1
BH391341|BH391341.1 AG-ND-107E4.TF ND-TAM Anopheles gambiae genomic clone AG-ND-107E4, DNA sequence.    56  0.001  1
CB909800|CB909800.1 USDA-FP_101543 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-22_B06 5', mRNA sequence.    36  0.053  3
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RA2) RecName: Full=Delta-1-pyrroline-5-carboxylate dehydroge...   458   e-127
CP001087_1672(CP001087|pid:none) Desulfobacterium autotrophicum ...   252   2e-65
CP000140_2188(CP000140|pid:none) Parabacteroides distasonis ATCC...   249   2e-64
EF157670_5(EF157670|pid:none) Uncultured bacterium CBNPD1 BAC cl...   245   2e-63
CU466930_1098(CU466930|pid:none) Candidatus Cloacamonas acidamin...   245   3e-63
(P78568) RecName: Full=Delta-1-pyrroline-5-carboxylate dehydroge...   243   1e-62
AP009380_1401(AP009380|pid:none) Porphyromonas gingivalis ATCC 3...   232   2e-59
CR522870_2491(CR522870|pid:none) Desulfotalea psychrophila LSv54...   231   3e-59
AF252630_1(AF252630|pid:none) Aspergillus nidulans delta-1-pyrro...   230   9e-59
CU207366_389(CU207366|pid:none) Gramella forsetii KT0803 complet...   228   3e-58
protein update 2009. 4.23
PSORT 

 psg: 0.75  gvh: 0.46  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  4.0 %: plasma membrane
	  4.0 %: nuclear

>> prediction for AFJ501 is mit
5' end seq. ID AFJ501F
5' end seq. 
>AFJ501F.Seq
CATTTTAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGCTAAC
ATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAGAAAA
TTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGTTGTT
GGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCATAAT
AAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGAAAAT
TCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGTTTTC
CTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTACTATG
CTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTATTGAT
TTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGCCAAT
TCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTAGAGGGTTATGTTGTAGCAATC
TCACCATTTAATTTCACAGCAATTGGTTTGAATTTAAGTTCAGCCCCAGCTTTAATGGGT
AATGTTGTCCTTTGGAAGCCTGCTTCAACTGCTGTCTTATC----------
Length of 5' end seq. 701
3' end seq. ID AFJ501Z
3' end seq. 
>AFJ501Z.Seq
----------AATTAAAGATAGATTAGGTTACTGGTGTTAAATCAAATGAAAAATGGGTC
AATCTGATGATTTCTCTTCATTCGTTTCAGCTGTCATCGATAAAAACTCTTTCAACAATA
TTCAATCATACATTGAACACGCTAAAGCTTCACCAGATGCTGAAATCATTGTCGGTGGTA
AATGTGATAGCTCTGTTGGTTGGTTTGTTGAACCAACTATCATCCTTGCCAAAGATCCAC
ATTACAAATCAATGGAAGAAGAAATTTTCGGTCCAGTCTTAACCATTTATGTTTATGAAG
ATTCTAAATTTGAAGAAACATTAAAAATCTGTGATGAAACTTCACCATATGCTTTAACTG
GTTCAATCTTTTCAACTTGTAGATATGCTATTGAAACCGCTCACAAATACTTGAAGAATG
CTGCTGGTAACTTTTATATCAATGATAAATGCACTGGTGCCGTCGTTGGTCAACAACCAT
TTGGTGGTTCTCGTGCTTCAGGTACAAACGATAAAGCTGGTTCATCATTAAATCTTTTAA
GATGGATCAGCGCCAGAACAATCAAAGAAAACTTTGTCCCACTCACAGTTTCACTATCCA
TACAGATNATCCAGAAAACTAA
Length of 3' end seq. 612
Connected seq. ID AFJ501P
Connected seq. 
>AFJ501P.Seq
CATTTTAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGCTAAC
ATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAGAAAA
TTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGTTGTT
GGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCATAAT
AAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGAAAAT
TCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGTTTTC
CTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTACTATG
CTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTATTGAT
TTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGCCAAT
TCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTAGAGGGTTATGTTGTAGCAATC
TCACCATTTAATTTCACAGCAATTGGTTTGAATTTAAGTTCAGCCCCAGCTTTAATGGGT
AATGTTGTCCTTTGGAAGCCTGCTTCAACTGCTGTCTTATC----------AATTAAAGA
TAGATTAGGTTACTGGTGTTAAATCAAATGAAAAATGGGTCAATCTGATGATTTCTCTTC
ATTCGTTTCAGCTGTCATCGATAAAAACTCTTTCAACAATATTCAATCATACATTGAACA
CGCTAAAGCTTCACCAGATGCTGAAATCATTGTCGGTGGTAAATGTGATAGCTCTGTTGG
TTGGTTTGTTGAACCAACTATCATCCTTGCCAAAGATCCACATTACAAATCAATGGAAGA
AGAAATTTTCGGTCCAGTCTTAACCATTTATGTTTATGAAGATTCTAAATTTGAAGAAAC
ATTAAAAATCTGTGATGAAACTTCACCATATGCTTTAACTGGTTCAATCTTTTCAACTTG
TAGATATGCTATTGAAACCGCTCACAAATACTTGAAGAATGCTGCTGGTAACTTTTATAT
CAATGATAAATGCACTGGTGCCGTCGTTGGTCAACAACCATTTGGTGGTTCTCGTGCTTC
AGGTACAAACGATAAAGCTGGTTCATCATTAAATCTTTTAAGATGGATCAGCGCCAGAAC
AATCAAAGAAAACTTTGTCCCACTCACAGTTTCACTATCCATACAGATNATCCAGAAAAC
TAA
Length of connected seq. 1313
Full length Seq ID -
Full length Seq. -
Length of full length seq. -